Gene Symbol: SSH1
Description: Ssh1p
Alias: Ssh1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Wittke S, Dünnwald M, Albertsen M, Johnsson N. Recognition of a subset of signal sequences by Ssh1p, a Sec61p-related protein in the membrane of endoplasmic reticulum of yeast Saccharomyces cerevisiae. Mol Biol Cell. 2002;13:2223-32 pubmed
    b>Ssh1p of Saccharomyces cerevisiae is related in sequence to Sec61p, a general receptor for signal sequences and the major subunit of the channel that guides proteins across the membrane of the endoplasmic reticulum...
  2. Junne T, Schwede T, Goder V, Spiess M. Mutations in the Sec61p channel affecting signal sequence recognition and membrane protein topology. J Biol Chem. 2007;282:33201-9 pubmed
    ..Our results suggest a model in which the regulated opening of the translocon is required for the faithful orientation of membrane proteins. ..
  3. Jiang Y, Cheng Z, Mandon E, Gilmore R. An interaction between the SRP receptor and the translocon is critical during cotranslational protein translocation. J Cell Biol. 2008;180:1149-61 pubmed publisher
    ..Disruption of the SBH2 gene, which encodes the beta subunit of the Ssh1p complex, likewise causes a growth defect when combined with SRbeta-DeltaTM...
  4. Becker T, Bhushan S, Jarasch A, Armache J, Funes S, Jossinet F, et al. Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. Science. 2009;326:1369-73 pubmed publisher
    ..This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation. ..
  5. Finke K, Plath K, Panzner S, Prehn S, Rapoport T, Hartmann E, et al. A second trimeric complex containing homologs of the Sec61p complex functions in protein transport across the ER membrane of S. cerevisiae. EMBO J. 1996;15:1482-94 pubmed discovered in the yeast endoplasmic reticulum membrane a second, structurally related trimeric complex, named Ssh1p complex...
  6. Wilkinson B, Tyson J, Stirling C. Ssh1p determines the translocation and dislocation capacities of the yeast endoplasmic reticulum. Dev Cell. 2001;1:401-9 pubmed
    ..However, the cellular role of the Sec61p homolog Ssh1p has not been clearly defined...
  7. Metzger M, Michaelis S. Analysis of quality control substrates in distinct cellular compartments reveals a unique role for Rpn4p in tolerating misfolded membrane proteins. Mol Biol Cell. 2009;20:1006-19 pubmed publisher
    ..Among these is RPN4, highlighting the importance of the Rpn4p-dependent response in tolerating UPR-M/C stress. Further analysis suggests the requirement for Rpn4p reflects severe impairment of the proteasome by UPR-M/C stress. ..
  8. Kingsbury J, McCusker J. Homoserine toxicity in Saccharomyces cerevisiae and Candida albicans homoserine kinase (thr1Delta) mutants. Eukaryot Cell. 2010;9:717-28 pubmed publisher
    ..Since the doa4Delta and proteasome mutants identified have reduced ubiquitin- and/or proteasome-mediated proteolysis, the degradation of a particular protein or subset of proteins likely contributes to homoserine toxicity...
  9. Trueman S, Mandon E, Gilmore R. Translocation channel gating kinetics balances protein translocation efficiency with signal sequence recognition fidelity. Mol Biol Cell. 2011;22:2983-93 pubmed publisher
    ..Our results indicate that the equilibrium between the open and closed conformations of the protein translocation channel maintains a balance between translocation activity and signal sequence recognition fidelity. ..

More Information


  1. Habeck G, Ebner F, Shimada Kreft H, Kreft S. The yeast ERAD-C ubiquitin ligase Doa10 recognizes an intramembrane degron. J Cell Biol. 2015;209:261-73 pubmed publisher
    ..Sbh2 is part of the trimeric Ssh1 complex involved in protein translocation. Unassembled Sbh2 is rapidly degraded in a Doa10-dependent manner...
  2. Schilke B, Forster J, Davis J, James P, Walter W, Laloraya S, et al. The cold sensitivity of a mutant of Saccharomyces cerevisiae lacking a mitochondrial heat shock protein 70 is suppressed by loss of mitochondrial DNA. J Cell Biol. 1996;134:603-13 pubmed
    b>SSH1, a newly identified member of the heat shock protein (hsp70) multigene family of the budding yeast Saccharomyces cerevisiae, encodes a protein localized to the mitochondrial matrix...
  3. Spiller M, Stirling C. Preferential targeting of a signal recognition particle-dependent precursor to the Ssh1p translocon in yeast. J Biol Chem. 2011;286:21953-60 pubmed publisher
    ..In yeast, two channels exist: the canonical Sec61p channel and a homolog called Ssh1p. Here, we used trapped translocation intermediates to demonstrate that a specific signal recognition particle-..
  4. Harty C, Römisch K. Analysis of Sec61p and Ssh1p interactions in the ER membrane using the split-ubiquitin system. BMC Cell Biol. 2013;14:14 pubmed publisher
    ..protein translocation channel in the endoplasmic reticulum (ER) membrane, and its non-essential homologue, Ssh1p, when fused C-terminally to CPLV are inactive...