Gene Symbol: SSE1
Description: adenyl-nucleotide exchange factor SSE1
Alias: LPG3, MSI3, adenyl-nucleotide exchange factor SSE1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Fan Q, Park K, Du Z, Morano K, Li L. The role of Sse1 in the de novo formation and variant determination of the [PSI+] prion. Genetics. 2007;177:1583-93 pubmed
    ..We report here that Sse1, the yeast ortholog of the mammalian heat-shock protein 110 (Hsp110) and a nucleotide exchange factor for Hsp70 ..
  2. Andréasson C, Fiaux J, Rampelt H, Druffel Augustin S, Bukau B. Insights into the structural dynamics of the Hsp110-Hsp70 interaction reveal the mechanism for nucleotide exchange activity. Proc Natl Acad Sci U S A. 2008;105:16519-24 pubmed publisher
    ..Here, we report the architecture of the complex between the yeast Hsp110, Sse1, and its cognate Hsp70 partner, Ssa1, as revealed by hydrogen-deuterium exchange analysis and site-specific cross-..
  3. Goeckeler J, Petruso A, Aguirre J, Clement C, Chiosis G, Brodsky J. The yeast Hsp110, Sse1p, exhibits high-affinity peptide binding. FEBS Lett. 2008;582:2393-6 pubmed publisher
    ..Moreover, an Hsp70-binding peptide is unable to associate with Sse1p, suggesting that Hsp70s and Hsp110s possess partially distinct peptide recognition motifs. ..
  4. Bagriantsev S, Gracheva E, Richmond J, Liebman S. Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition. Mol Biol Cell. 2008;19:2433-43 pubmed publisher
    ..Using a candidate approach, we detected Hsp104, Ssb1/2, Sis1, Sse1, Ydj1, and Sla2 among minor components of the aggregates...
  5. Albanèse V, Yam A, Baughman J, Parnot C, Frydman J. Systems analyses reveal two chaperone networks with distinct functions in eukaryotic cells. Cell. 2006;124:75-88 pubmed
    ..The emergence of a translation-linked chaperone network likely underlies the elaborate cotranslational folding process necessary for the evolution of larger multidomain proteins characteristic of eukaryotic cells. ..
  6. Kryndushkin D, Wickner R. Nucleotide exchange factors for Hsp70s are required for [URE3] prion propagation in Saccharomyces cerevisiae. Mol Biol Cell. 2007;18:2149-54 pubmed
    ..Sse1p and Fes1p are nucleotide exchange factors for Ssa1p. Interestingly, deletion of either SSE1 or FES1 completely blocked [URE3] propagation...
  7. Heck J, Cheung S, Hampton R. Cytoplasmic protein quality control degradation mediated by parallel actions of the E3 ubiquitin ligases Ubr1 and San1. Proc Natl Acad Sci U S A. 2010;107:1106-11 pubmed publisher
    ..The broad conservation of Ubr ligases and the relevant chaperones indicates that these mechanisms will be important in understanding both basic and biomedical aspects of cellular proteostasis. ..
  8. Sadlish H, Rampelt H, Shorter J, Wegrzyn R, Andréasson C, Lindquist S, et al. Hsp110 chaperones regulate prion formation and propagation in S. cerevisiae by two discrete activities. PLoS ONE. 2008;3:e1763 pubmed publisher
    ..Recently, the Hsp110 protein, Sse1, has been identified as a nucleotide exchange factor (NEF) for both cytosolic Hsp70 chaperone family members, Ssa1 ..
  9. Andréasson C, Fiaux J, Rampelt H, Mayer M, Bukau B. Hsp110 is a nucleotide-activated exchange factor for Hsp70. J Biol Chem. 2008;283:8877-84 pubmed publisher
    ..Here, we show that the NEF activity of the yeast Hsp110 homologue Sse1 itself is controlled by nucleotide...

More Information


  1. Polier S, Dragovic Z, Hartl F, Bracher A. Structural basis for the cooperation of Hsp70 and Hsp110 chaperones in protein folding. Cell. 2008;133:1068-79 pubmed publisher
  2. Dragovic Z, Broadley S, Shomura Y, Bracher A, Hartl F. Molecular chaperones of the Hsp110 family act as nucleotide exchange factors of Hsp70s. EMBO J. 2006;25:2519-28 pubmed
    ..Similarly, deletion of SSE1 causes a firefly luciferase folding defect in yeast cells under heat stress in vivo...
  3. Liu Q, Hendrickson W. Insights into Hsp70 chaperone activity from a crystal structure of the yeast Hsp110 Sse1. Cell. 2007;131:106-20 pubmed
    ..The 2.4 A resolution structure of Sse1 with ATP shows that Hsp110s are indeed Hsp70 relatives, and it provides insight into allosteric coupling between ..
  4. Goeckeler J, Stephens A, Lee P, Caplan A, Brodsky J. Overexpression of yeast Hsp110 homolog Sse1p suppresses ydj1-151 thermosensitivity and restores Hsp90-dependent activity. Mol Biol Cell. 2002;13:2760-70 pubmed
    ..we screened for multicopy suppressors of the temperature-sensitive ydj1-151 mutant and identified a yeast Hsp110, SSE1. Overexpression of Sse1p also suppressed the folding defect of v-Src kinase in the ydj1-151 mutant and partially ..
  5. Lee P, Shabbir A, Cardozo C, Caplan A. Sti1 and Cdc37 can stabilize Hsp90 in chaperone complexes with a protein kinase. Mol Biol Cell. 2004;15:1785-92 pubmed
    ..These data suggest that Cdc37 and Sti1 have functional overlap in stabilizing Hsp90:client complexes. Finally, we show that Cns1 functions in MAP kinase signaling in association with Cpr7. ..
  6. Liu X, Morano K, Thiele D. The yeast Hsp110 family member, Sse1, is an Hsp90 cochaperone. J Biol Chem. 1999;274:26654-60 pubmed
    ..Heat shock induction of SSE1, encoding a member of the Hsp110 family of heat shock proteins, was also dependent on the HSF CTA...
  7. Shaner L, Trott A, Goeckeler J, Brodsky J, Morano K. The function of the yeast molecular chaperone Sse1 is mechanistically distinct from the closely related hsp70 family. J Biol Chem. 2004;279:21992-2001 pubmed
    The Sse1/Hsp110 molecular chaperones are a poorly understood subgroup of the Hsp70 chaperone family...
  8. Shaner L, Wegele H, Buchner J, Morano K. The yeast Hsp110 Sse1 functionally interacts with the Hsp70 chaperones Ssa and Ssb. J Biol Chem. 2005;280:41262-9 pubmed
    ..However, relatively little is known regarding the interactions and cellular functions of Sse1, the yeast Hsp110 homolog...
  9. Trott A, Shaner L, Morano K. The molecular chaperone Sse1 and the growth control protein kinase Sch9 collaborate to regulate protein kinase A activity in Saccharomyces cerevisiae. Genetics. 2005;170:1009-21 pubmed
    ..Hsp90 functions in concert with a number of cochaperones, including the Hsp110 homolog Sse1. In this report, we demonstrate a novel synthetic genetic interaction between SSE1 and SCH9...
  10. Shaner L, Sousa R, Morano K. Characterization of Hsp70 binding and nucleotide exchange by the yeast Hsp110 chaperone Sse1. Biochemistry. 2006;45:15075-84 pubmed
    b>SSE1 and SSE2 encode the essential yeast members of the Hsp70-related Hsp110 molecular chaperone family. Both mammalian Hsp110 and the Sse proteins functionally interact with cognate cytosolic Hsp70s as nucleotide exchange factors...
  11. Hrizo S, Gusarova V, Habiel D, Goeckeler J, Fisher E, Brodsky J. The Hsp110 molecular chaperone stabilizes apolipoprotein B from endoplasmic reticulum-associated degradation (ERAD). J Biol Chem. 2007;282:32665-75 pubmed
    ..This study indicates that chaperones within distinct complexes can play unique roles during ER-associated degradation (ERAD), establishes a role for Sse1/Hsp110 in ERAD, and identifies Hsp110 as a target to lower cholesterol.
  12. Mandal A, Gibney P, Nillegoda N, Theodoraki M, Caplan A, Morano K. Hsp110 chaperones control client fate determination in the hsp70-Hsp90 chaperone system. Mol Biol Cell. 2010;21:1439-48 pubmed publisher
    ..The Hsp110 chaperone Sse1 promotes Hsp90 activity in yeast, and functions as a nucleotide exchange factor (NEF) for cytosolic Hsp70, but the ..
  13. Schuermann J, Jiang J, Cuellar J, Llorca O, Wang L, Gimenez L, et al. Structure of the Hsp110:Hsc70 nucleotide exchange machine. Mol Cell. 2008;31:232-43 pubmed publisher
    ..The symmetrical interactions in the complex may model other Hsp70 family heterodimers in which two Hsp70s reciprocally act as NEFs. ..
  14. Shaner L, Gibney P, Morano K. The Hsp110 protein chaperone Sse1 is required for yeast cell wall integrity and morphogenesis. Curr Genet. 2008;54:1-11 pubmed publisher
    ..The eukaryotic chaperone Hsp110 is represented by the SSE1/2 genes in Saccharomyces cerevisiae, which act as nucleotide exchange factors (NEFs) for cognate cytosolic Hsp70 ..
  15. Yam A, Albanèse V, Lin H, Frydman J. Hsp110 cooperates with different cytosolic HSP70 systems in a pathway for de novo folding. J Biol Chem. 2005;280:41252-61 pubmed
    ..In yeast, these include the Hsp70s SSB and SSA as well as the Hsp110-like Sse1/2p. The cellular functions and interplay between these different Hsp70 systems remain ill-defined...
  16. Raviol H, Sadlish H, Rodriguez F, Mayer M, Bukau B. Chaperone network in the yeast cytosol: Hsp110 is revealed as an Hsp70 nucleotide exchange factor. EMBO J. 2006;25:2510-8 pubmed
    ..Overexpression of the only other described cytosolic NEF, Fes1p, can partially compensate for a lethal sse1,2Delta phenotype, however, the cells are sensitive to stress conditions...
  17. Shirayama M, Kawakami K, Matsui Y, Tanaka K, Toh e A. MSI3, a multicopy suppressor of mutants hyperactivated in the RAS-cAMP pathway, encodes a novel HSP70 protein of Saccharomyces cerevisiae. Mol Gen Genet. 1993;240:323-32 pubmed
    The MSI3 gene was isolated as a multicopy suppressor of the heat shock-sensitive phenotype of the ira1 mutation, which causes hyperactivation of the RAS-cAMP pathway...
  18. Zhou W, Ryan J, Zhou H. Global analyses of sumoylated proteins in Saccharomyces cerevisiae. Induction of protein sumoylation by cellular stresses. J Biol Chem. 2004;279:32262-8 pubmed
    ..Taken together, these results show that protein sumoylation is broadly involved in many cellular functions and this mass spectrometry-based proteomic approach is useful in studying the regulation of protein sumoylation in the cells. ..
  19. O Driscoll J, Clare D, Saibil H. Prion aggregate structure in yeast cells is determined by the Hsp104-Hsp110 disaggregase machinery. J Cell Biol. 2015;211:145-58 pubmed publisher
    ..Both Hsp104 (an Hsp100 disaggregase) and Sse1 (the major yeast form of Hsp110) were localized to this surface shell of [PSI+] deposits in the deletion mutants...
  20. Abrams J, Verghese J, Gibney P, Morano K. Hierarchical functional specificity of cytosolic heat shock protein 70 (Hsp70) nucleotide exchange factors in yeast. J Biol Chem. 2014;289:13155-67 pubmed publisher
    ..Hsp70 interacts with up to three types of nucleotide exchange factors (NEFs) homologous to human counterparts: Sse1/Sse2 (Heat shock protein 110 (Hsp110)), Fes1 (HspBP1), and Snl1 (Bag-1)...
  21. Tenge V, Knowles J, Johnson J. The ribosomal biogenesis protein Utp21 interacts with Hsp90 and has differing requirements for Hsp90-associated proteins. PLoS ONE. 2014;9:e92569 pubmed publisher
  22. Moran C, Kinsella G, Zhang Z, Perrett S, Jones G. Mutational analysis of Sse1 (Hsp110) suggests an integral role for this chaperone in yeast prion propagation in vivo. G3 (Bethesda). 2013;3:1409-18 pubmed publisher
    The yeast Hsp110 chaperone Sse1 is a conserved protein that is a noncanonical member of the Hsp70 protein superfamily...
  23. Koplin A, Preissler S, Ilina Y, Koch M, Scior A, Erhardt M, et al. A dual function for chaperones SSB-RAC and the NAC nascent polypeptide-associated complex on ribosomes. J Cell Biol. 2010;189:57-68 pubmed publisher
    ..Furthermore, NAC mutations revealed genetic interaction with a deletion of Sse1, a nucleotide exchange factor regulating the cytosolic Hsp70 network...
  24. Martineau C, Beckerich J, Kabani M. Flo11p-independent control of "mat" formation by hsp70 molecular chaperones and nucleotide exchange factors in yeast. Genetics. 2007;177:1679-89 pubmed
    ..Importantly, these mutations did not compromise invasive growth or Flo11p expression, suggesting that Flo11p-independent pathways are necessary to form mats. ..
  25. Gowda N, Kandasamy G, Froehlich M, Dohmen R, Andréasson C. Hsp70 nucleotide exchange factor Fes1 is essential for ubiquitin-dependent degradation of misfolded cytosolic proteins. Proc Natl Acad Sci U S A. 2013;110:5975-80 pubmed publisher
    ..Our findings reveal that Hsp70 direct proteins toward either folding or degradation by using distinct nucleotide exchange factors. ..
  26. Keefer K, True H. A toxic imbalance of Hsp70s in Saccharomyces cerevisiae is caused by competition for cofactors. Mol Microbiol. 2017;105:860-868 pubmed publisher
    ..We discovered that overexpression of the nucleotide exchange factor Sse1 can partially alleviate this toxicity...
  27. Staresincic L, Walker J, Dirac Svejstrup A, Mitter R, Svejstrup J. GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein. J Biol Chem. 2011;286:35553-61 pubmed publisher
    ..Together, our data suggest that Npa3 defines an unconventional pathway for nuclear import of RNAPII, which involves GTP-dependent binding of Npa3 to the polymerase. ..
  28. Moriel Carretero M, Tous C, Aguilera A. Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1. Proc Natl Acad Sci U S A. 2011;108:15300-5 pubmed publisher
    ..Our results provide evidence for a role of chaperones in NER and transcription, with implications in cancer and TFIIH-associated syndromes. ..
  29. Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher
    ..Thus, cells must have a way of ensuring a proper balance between actin assembly pathways. ..
  30. Sugiyama T, Nobuta R, Ando K, Matsuki Y, Inada T. Crucial role of ATP-bound Sse1 in Upf1-dependent degradation of the truncated product. Biochem Biophys Res Commun. 2017;488:122-128 pubmed publisher
    ..Here we report that Sse1, a member of the Hsp110 family, and Hsp70 play a crucial role in Upf-dependent degradation of the truncated FLAG-..
  31. Andréasson C, Rampelt H, Fiaux J, Druffel Augustin S, Bukau B. The endoplasmic reticulum Grp170 acts as a nucleotide exchange factor of Hsp70 via a mechanism similar to that of the cytosolic Hsp110. J Biol Chem. 2010;285:12445-53 pubmed publisher
    ..In this study, we compare the yeast Grp170 Lhs1 with the yeast Hsp110 Sse1. We find that residues important for Sse1 NEF activity are conserved in Lhs1 and that mutations in these residues ..
  32. Sahi C, Kominek J, Ziegelhoffer T, Yu H, Baranowski M, Marszalek J, et al. Sequential duplications of an ancient member of the DnaJ-family expanded the functional chaperone network in the eukaryotic cytosol. Mol Biol Evol. 2013;30:985-98 pubmed publisher
  33. Sasaki T, Toh e A, Kikuchi Y. Extragenic suppressors that rescue defects in the heat stress response of the budding yeast mutant tom1. Mol Gen Genet. 2000;262:940-8 pubmed
    ..groups and six of the genes were identified: tmr1/cyr1, tmnr2/sch9, tmr3/zuo1, tmr4, tmr5/mot1, tmr6/sse1, tmr7 and tmr8/kre6...
  34. Phan V, Ding V, Li F, Chalkley R, Burlingame A, McCormick F. The RasGAP proteins Ira2 and neurofibromin are negatively regulated by Gpb1 in yeast and ETEA in humans. Mol Cell Biol. 2010;30:2264-79 pubmed publisher
    ..These findings provide evidence for conserved ubiquitination pathways regulating the RasGAP proteins Ira2 (in yeast) and neurofibromin (in humans). ..
  35. Mészáros N, Cibulka J, Mendiburo M, Romanauska A, Schneider M, Köhler A. Nuclear pore basket proteins are tethered to the nuclear envelope and can regulate membrane curvature. Dev Cell. 2015;33:285-98 pubmed publisher
    ..Basket amphipathic helices are functionally linked to distinct transmembrane nucleoporins of the NPC core, suggesting a key contribution to the membrane remodeling events that underlie NPC assembly. ..
  36. Chartron J, Gonzalez G, Clemons W. A structural model of the Sgt2 protein and its interactions with chaperones and the Get4/Get5 complex. J Biol Chem. 2011;286:34325-34 pubmed publisher
    ..These results allow us to present a structural model of the Sgt2-Get4/Get5-HSC complex. ..
  37. Makhnevych T, Houry W. The control of spindle length by Hsp70 and Hsp110 molecular chaperones. FEBS Lett. 2013;587:1067-72 pubmed publisher
    ..The modulation of spindle length by molecular chaperones might be a mechanism by which cell division can be controlled especially under proteostatic stress. ..
  38. Makhnevych T, Wong P, Pogoutse O, Vizeacoumar F, Greenblatt J, Emili A, et al. Hsp110 is required for spindle length control. J Cell Biol. 2012;198:623-36 pubmed publisher
    ..The role of Hsp110 Sse1 as a nucleotide exchange factor for the Hsp70 chaperones Ssa1/Ssa2 was found to be required for maintaining the ..
  39. Garcia V, Nillegoda N, Bukau B, Morano K. Substrate binding by the yeast Hsp110 nucleotide exchange factor and molecular chaperone Sse1 is not obligate for its biological activities. Mol Biol Cell. 2017;28:2066-2075 pubmed publisher
    ..The related protein Hsp110 (Sse1/Sse2 in Saccharomyces cerevisiae) functions as a nucleotide exchange factor (NEF) to regulate the protein ..
  40. Hamdan N, Kritsiligkou P, Grant C. ER stress causes widespread protein aggregation and prion formation. J Cell Biol. 2017;216:2295-2304 pubmed publisher
    ..The onset of ER stress is known to correlate with various disease processes, and our data indicate that widespread amorphous and amyloid protein aggregation is an unanticipated outcome of such stress. ..