Gene Symbol: SRB7
Description: Srb7p
Alias: MED21, SSX1, Srb7p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Han S, Lee Y, Gim B, Ryu G, Park S, Lane W, et al. Activator-specific requirement of yeast mediator proteins for RNA polymerase II transcriptional activation. Mol Cell Biol. 1999;19:979-88 pubmed
  2. Myers L, Gustafsson C, Bushnell D, Lui M, Erdjument Bromage H, Tempst P, et al. The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain. Genes Dev. 1998;12:45-54 pubmed
    ..Evidence for human homologs of several mediator proteins, including Med7, points to similar mechanisms in higher cells. ..
  3. Lariviere L, Seizl M, van Wageningen S, Röther S, van de Pasch L, Feldmann H, et al. Structure-system correlation identifies a gene regulatory Mediator submodule. Genes Dev. 2008;22:872-7 pubmed publisher
    ..The presented structure-based system perturbation is superior to gene deletion analysis of gene regulation. ..
  4. Li L, Quinton T, Miles S, Breeden L. Genetic interactions between mediator and the late G1-specific transcription factor Swi6 in Saccharomyces cerevisiae. Genetics. 2005;171:477-88 pubmed
    ..A truncated form of the essential Srb7 mediator subunit also suppresses swi6 mutations and shows a defect in recruitment of the tail module components ..
  5. Koschubs T, Lorenzen K, Baumli S, Sandström S, Heck A, Cramer P. Preparation and topology of the Mediator middle module. Nucleic Acids Res. 2010;38:3186-95 pubmed publisher
    ..The subunits, Med1 and Med10, which bridge to the Mediator tail module, bind to both Med7 and Med4. ..
  6. Gromöller A, Lehming N. Srb7p is essential for the activation of a subset of genes. FEBS Lett. 2000;484:48-54 pubmed
    ..The role of the essential mediator component Srb7p has been difficult to investigate, since no conditional lethal allele has been available to date...
  7. Lee Y, Park J, Min S, Han S, Kim Y. An activator binding module of yeast RNA polymerase II holoenzyme. Mol Cell Biol. 1999;19:2967-76 pubmed
  8. Han S, Lee J, Kang J, Kim Y. Med9/Cse2 and Gal11 modules are required for transcriptional repression of distinct group of genes. J Biol Chem. 2001;276:37020-6 pubmed
  9. Beve J, Hu G, Myers L, Balciunas D, Werngren O, Hultenby K, et al. The structural and functional role of Med5 in the yeast Mediator tail module. J Biol Chem. 2005;280:41366-72 pubmed
    ..We discuss a possible role for Med5 in coordinating nuclear and mitochondrial gene transcription. ..

More Information


  1. Singh H, Erkine A, Kremer S, Duttweiler H, Davis D, Iqbal J, et al. A functional module of yeast mediator that governs the dynamic range of heat-shock gene expression. Genetics. 2006;172:2169-84 pubmed
    ..Three of the six subunits, Med7, Med10/Nut2, and Med21/Srb7, map to Mediator's middle domain; two subunits, Med14/Rgr1 and Med16/Sin4, to its tail domain; and one ..
  2. Cai G, Imasaki T, Takagi Y, Asturias F. Mediator structural conservation and implications for the regulation mechanism. Structure. 2009;17:559-67 pubmed publisher
    ..This suggests that the topology and structural dynamics of Mediator constitute important elements of a conserved regulation mechanism. ..
  3. Gromöller A, Lehming N. Srb7p is a physical and physiological target of Tup1p. EMBO J. 2000;19:6845-52 pubmed
    ..We demonstrate that the essential holoenzyme component Srb7p is a physiologically relevant target of the global repressor Tup1p in Saccharomyces cerevisiae...
  4. Baumli S, Hoeppner S, Cramer P. A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer. J Biol Chem. 2005;280:18171-8 pubmed
    ..We present the 3.0-A crystal structure of a highly conserved part of the Mediator, the MED7.MED21 (Med7.Srb7) heterodimer...
  5. Seizl M, Lariviere L, Pfaffeneder T, Wenzeck L, Cramer P. Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization. Nucleic Acids Res. 2011;39:6291-304 pubmed publisher
    ..The bundle domain thus represents a common building block that has been multiplied and functionally diversified during Mediator evolution in eukaryotes. ..
  6. Jedidi I, Zhang F, Qiu H, Stahl S, Palmer I, Kaufman J, et al. Activator Gcn4 employs multiple segments of Med15/Gal11, including the KIX domain, to recruit mediator to target genes in vivo. J Biol Chem. 2010;285:2438-55 pubmed publisher
    ..Gcn4 is distinctive in relying on comparable contributions from multiple segments of Gal11 for efficient recruitment of Mediator in vivo. ..
  7. Liu Z, Myers L. Med5(Nut1) and Med17(Srb4) are direct targets of mediator histone H4 tail interactions. PLoS ONE. 2012;7:e38416 pubmed publisher
    ..This analysis has identified the Med5 subunit of Mediator as a target for histone tail interactions and suggests that the previously observed effect of med5 mutations on telomeric heterochromatin and silencing is direct. ..
  8. Sinturel F, Navickas A, Wery M, Descrimes M, Morillon A, Torchet C, et al. Cytoplasmic Control of Sense-Antisense mRNA Pairs. Cell Rep. 2015;12:1853-64 pubmed publisher
  9. Eychenne T, Novikova E, Barrault M, Alibert O, Boschiero C, Peixeiro N, et al. Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture. Genes Dev. 2016;30:2119-2132 pubmed
    ..This study thus provides mechanistic insights into the coordinated function of Mediator and TFIIB in PIC assembly in different chromatin contexts. ..
  10. Hallberg M, Hu G, Tronnersjö S, Shaikhibrahim Z, Balciunas D, Bjorklund S, et al. Functional and physical interactions within the middle domain of the yeast mediator. Mol Genet Genomics. 2006;276:197-210 pubmed
    b>Med21 (Srb7) is a small essential subunit of the middle domain of the Mediator, which is conserved in all eukaryotes. It is thought to play an important role in both transcriptional activation and repression...
  11. Chanet R, Heude M. Characterization of mutations that are synthetic lethal with pol3-13, a mutated allele of DNA polymerase delta in Saccharomyces cerevisiae. Curr Genet. 2003;43:337-50 pubmed
    ..involved in repair and genome stability (MET18/ MMS19), in the control of origin-firing and/or transcription (ABF1, SRB7), in the S/G2 checkpoint (RAD53), in the Ras-cAMP signal transduction pathway (MKS1), in nuclear pore metabolism (..
  12. Balciunas D, Hallberg M, Bjorklund S, Ronne H. Functional interactions within yeast mediator and evidence of differential subunit modifications. J Biol Chem. 2003;278:3831-9 pubmed
    ..interactions within Mediator, we have tested such fusions of the lexA DNA binding domain to Med1, Med2, Gal11, Srb7, and Srb10 in wild type, med1, med2, gal11, sin4, srb8, srb10, and srb11 strains...
  13. Ang K, Ee G, Ang E, Koh E, Siew W, Chan Y, et al. Mediator acts upstream of the transcriptional activator Gal4. PLoS Biol. 2012;10:e1001290 pubmed publisher
    ..The ability of Mediator to control the protein degradation of transcriptional inhibitors indicates that Mediator is actually able to direct its own recruitment to gene promoters. ..
  14. Park J, Kim H, Han S, Hwang M, Lee Y, Kim Y. In vivo requirement of activator-specific binding targets of mediator. Mol Cell Biol. 2000;20:8709-19 pubmed
  15. Miller C, Matic I, Maier K, Schwalb B, Roether S, Strasser K, et al. Mediator phosphorylation prevents stress response transcription during non-stress conditions. J Biol Chem. 2012;287:44017-26 pubmed publisher
    ..Thus dynamic and differential Mediator phosphorylation contributes to gene regulation in eukaryotic cells. ..
  16. Eyboulet F, Wydau Dematteis S, Eychenne T, Alibert O, Neil H, Boschiero C, et al. Mediator independently orchestrates multiple steps of preinitiation complex assembly in vivo. Nucleic Acids Res. 2015;43:9214-31 pubmed publisher
    ..This study provides an extensive genome-wide view of Mediator's role in PIC formation, suggesting that Mediator coordinates multiple steps of a PIC assembly pathway. ..
  17. Lee Y, Kim Y. Requirement for a functional interaction between mediator components Med6 and Srb4 in RNA polymerase II transcription. Mol Cell Biol. 1998;18:5364-70 pubmed
    ..Our results suggest not only the existence of a specific interaction between Med6 and Srb4 but also the requirement of this interaction in transcriptional regulation of RNA polymerase II holoenzyme. ..
  18. Swanson M, Qiu H, Sumibcay L, Krueger A, Kim S, Natarajan K, et al. A multiplicity of coactivators is required by Gcn4p at individual promoters in vivo. Mol Cell Biol. 2003;23:2800-20 pubmed
    ..We observed considerable differences in coactivator requirements among several Gcn4p-dependent promoters; thus, only a subset of the array of coactivators that can be recruited by Gcn4p is required at a given target gene in vivo. ..
  19. Natarajan K, Jackson B, Rhee E, Hinnebusch A. yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex. Mol Cell. 1998;2:683-92 pubmed
    ..Our results provide strong evidence that recruitment of SAGA, in addition to holoenzyme, is crucial for activation by Gcn4p in vivo and that yTAFII61 plays a key role in this process. ..