Gene Symbol: SPO11
Description: Spo11p
Alias: Spo11p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. McKee A, Kleckner N. A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics. 1997;146:797-816 pubmed
    ..a gene required for initiation of meiotic recombination and development of meiosis-specific chromosome structure (SPO11)...
  2. Zanders S, Sonntag Brown M, Chen C, Alani E. Pch2 modulates chromatid partner choice during meiotic double-strand break repair in Saccharomyces cerevisiae. Genetics. 2011;188:511-21 pubmed publisher
    ..We propose a model in which Pch2 is required to promote full Mek1 activity and thereby promotes interhomolog repair...
  3. Malone R, Bullard S, Hermiston M, Rieger R, Cool M, Galbraith A. Isolation of mutants defective in early steps of meiotic recombination in the yeast Saccharomyces cerevisiae. Genetics. 1991;128:79-88 pubmed
    ..Analysis of 56 of these has generated alleles of the known Rec genes SPO11, ME14 and MER1, as well as defining five new genes: REC102, REC104, REC107, REC113 and REC114...
  4. Argunhan B, Farmer S, Leung W, Terentyev Y, Humphryes N, Tsubouchi T, et al. Direct and indirect control of the initiation of meiotic recombination by DNA damage checkpoint mechanisms in budding yeast. PLoS ONE. 2013;8:e65875 pubmed publisher
    ..We also identified prophase I exit, which is under the control of the DNA damage checkpoint machinery, to be a critical event associated with down-regulating meiotic DSB formation. ..
  5. Gray S, Allison R, Garcia V, Goldman A, Neale M. Positive regulation of meiotic DNA double-strand break formation by activation of the DNA damage checkpoint kinase Mec1(ATR). Open Biol. 2013;3:130019 pubmed publisher
    ..Meiotic DSB formation is a complex process, requiring numerous proteins, of which Spo11 is the evolutionarily conserved catalytic subunit...
  6. Maleki S, Neale M, Arora C, Henderson K, Keeney S. Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae. Chromosoma. 2007;116:471-86 pubmed
    ..reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein...
  7. Buonomo S, Clyne R, Fuchs J, Loidl J, Uhlmann F, Nasmyth K. Disjunction of homologous chromosomes in meiosis I depends on proteolytic cleavage of the meiotic cohesin Rec8 by separin. Cell. 2000;103:387-98 pubmed
    ..We show here that cleavage of Rec8 by separin at one of two different sites is necessary for the resolution of chiasmata and the disjunction of homologous chromosomes during meiosis. ..
  8. Brar G, Yassour M, Friedman N, Regev A, Ingolia N, Weissman J. High-resolution view of the yeast meiotic program revealed by ribosome profiling. Science. 2012;335:552-7 pubmed publisher
    ..This work reveals pervasive translational control in meiosis and helps to illuminate the molecular basis of the broad restructuring of meiotic cells. ..
  9. Oelschlaegel T, Schwickart M, Matos J, Bogdanova A, Camasses A, Havlis J, et al. The yeast APC/C subunit Mnd2 prevents premature sister chromatid separation triggered by the meiosis-specific APC/C-Ama1. Cell. 2005;120:773-88 pubmed
    ..In vitro, Mnd2 inhibits ubiquitination of Pds1 by APC/C-Ama1 but not by other APC/C holo-enzymes. We conclude that chromosome segregation in meiosis depends on the selective inhibition of a meiosis-specific form of the APC/C. ..

More Information


  1. Cha R, Weiner B, Keeney S, Dekker J, Kleckner N. Progression of meiotic DNA replication is modulated by interchromosomal interaction proteins, negatively by Spo11p and positively by Rec8p. Genes Dev. 2000;14:493-503 pubmed
    b>Spo11p is a key mediator of interhomolog interactions during meiosis. Deletion of the SPO11 gene decreases the length of S phase by approximately 25%. Rec8p is a key coordinator of meiotic interhomolog and intersister interactions...
  2. Farmer S, Hong E, Leung W, Argunhan B, Terentyev Y, Humphryes N, et al. Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS ONE. 2012;7:e39724 pubmed publisher
    ..The DSB reduction is further pronounced when pch2 is combined with a hypomorphic allele of SPO11. Interestingly, the level of DSB reduction is highly variable between chromosomes, with minimal impact on small ..
  3. MacQueen A, Roeder G. Fpr3 and Zip3 ensure that initiation of meiotic recombination precedes chromosome synapsis in budding yeast. Curr Biol. 2009;19:1519-26 pubmed publisher
    ..These data suggest a role for centromeres in coordinating major meiotic chromosomal events and draw an interesting parallel between yeast centromeres and C. elegans pairing centers. ..
  4. Sawarynski K, Najor N, Kepsel A, Brush G. Sic1-induced DNA rereplication during meiosis. Proc Natl Acad Sci U S A. 2009;106:232-7 pubmed publisher
    ..Our results provide strong evidence that CDK activity is required to prevent inappropriate initiation of DNA synthesis before the meiotic divisions. ..
  5. Rockmill B, Voelkel Meiman K, Roeder G. Centromere-proximal crossovers are associated with precocious separation of sister chromatids during meiosis in Saccharomyces cerevisiae. Genetics. 2006;174:1745-54 pubmed
    ..When crossing over in sgs1 is reduced by the introduction of a nonnull allele of SPO11, spore viability is improved, suggesting that the increased PSSC is due to increased crossing over...
  6. Keeney S, Giroux C, Kleckner N. Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell. 1997;88:375-84 pubmed
    ..We have purified these protein-DNA complexes and identified the protein as Spo11, one of several proteins required for DSB formation...
  7. Kosaka H, Shinohara M, Shinohara A. Csm4-dependent telomere movement on nuclear envelope promotes meiotic recombination. PLoS Genet. 2008;4:e1000196 pubmed publisher
    ..We propose that chromosome movement and associated telomere dynamics at the nuclear envelope promotes the completion of key biochemical steps during meiotic recombination. ..
  8. Cao L, Alani E, Kleckner N. A pathway for generation and processing of double-strand breaks during meiotic recombination in S. cerevisiae. Cell. 1990;61:1089-101 pubmed
    ..with the hot spot and that occurrence of these breaks depends upon meiotic recombination functions RAD50 and SPO11. Furthermore, these breaks occur in a processed form in wild-type cells and in a discrete, unprocessed form in ..
  9. Jiao K, Salem L, Malone R. Support for a meiotic recombination initiation complex: interactions among Rec102p, Rec104p, and Spo11p. Mol Cell Biol. 2003;23:5928-38 pubmed
    ..It has recently been shown that Spo11p coimmunoprecipitates with Rec102p in meiosis as well...
  10. Sommermeyer V, Béneut C, Chaplais E, Serrentino M, Borde V. Spp1, a member of the Set1 Complex, promotes meiotic DSB formation in promoters by tethering histone H3K4 methylation sites to chromosome axes. Mol Cell. 2013;49:43-54 pubmed publisher
    ..Programmed DNA double-strand breaks (DSBs) that initiate meiotic recombination, catalyzed by Spo11 and accessory DSB proteins, form in loop sequences in promoters, whereas the DSB proteins are located on ..
  11. Rabitsch K, Toth A, Galova M, Schleiffer A, Schaffner G, Aigner E, et al. A screen for genes required for meiosis and spore formation based on whole-genome expression. Curr Biol. 2001;11:1001-9 pubmed
    ..Reverse genetics using gene expression data is an effective method for identifying new genes involved in specific cellular processes. ..
  12. Refolio E, Cavero S, Marcon E, Freire R, San Segundo P. The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates. J Cell Sci. 2011;124:2488-500 pubmed publisher
    ..Formation of meiotic Ddc2 foci requires the generation of Spo11-dependent DNA double-strand breaks (DSBs), and is impaired in an RPA mutant...
  13. Mieczkowski P, Dominska M, Buck M, Lieb J, Petes T. Loss of a histone deacetylase dramatically alters the genomic distribution of Spo11p-catalyzed DNA breaks in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2007;104:3955-60 pubmed
    ..Some of the genes with altered frequencies of DSBs (including the ribosomal RNA gene cluster) are known targets of Sir2p deacetylation in the wild-type strain. ..
  14. Sasanuma H, Murakami H, Fukuda T, Shibata T, Nicolas A, Ohta K. Meiotic association between Spo11 regulated by Rec102, Rec104 and Rec114. Nucleic Acids Res. 2007;35:1119-33 pubmed
    Meiotic recombination is initiated by DNA double-stranded break (DSB) formation catalyzed by Spo11, a type-II topoisomerase-like transesterificase, presumably via a dimerization-mediated mechanism...
  15. Nag D, Pata J, Sironi M, Flood D, Hart A. Both conserved and non-conserved regions of Spo11 are essential for meiotic recombination initiation in yeast. Mol Genet Genomics. 2006;276:313-21 pubmed
    ..However, the molecular roles of these proteins are not clearly understood. The meiosis-specific Spo11 protein, which shows sequence similarity with a subunit of an archaeal topoisomerase, is believed to catalyze the ..
  16. Rabitsch K, Petronczki M, Javerzat J, Genier S, Chwalla B, Schleiffer A, et al. Kinetochore recruitment of two nucleolar proteins is required for homolog segregation in meiosis I. Dev Cell. 2003;4:535-48 pubmed
    ..Csm1 and Pcs1 might clamp together microtubule binding sites on the same (Pcs1) or sister (Csm1) kinetochores...
  17. Kee K, Keeney S. Functional interactions between SPO11 and REC102 during initiation of meiotic recombination in Saccharomyces cerevisiae. Genetics. 2002;160:111-22 pubmed
    ..b>Spo11p is thought to be the catalytic subunit of the DNA cleaving activity, but the roles of the other proteins, and the ..
  18. Lin W, Wang M, Jin H, Yu H. Cohesin plays a dual role in gene regulation and sister-chromatid cohesion during meiosis in Saccharomyces cerevisiae. Genetics. 2011;187:1041-51 pubmed publisher
    ..Our results suggest that the cohesin complex plays a dual role in gene regulation and sister-chromatid cohesion during meiotic differentiation in yeast. ..
  19. Kaliraman V, Mullen J, Fricke W, Bastin Shanower S, Brill S. Functional overlap between Sgs1-Top3 and the Mms4-Mus81 endonuclease. Genes Dev. 2001;15:2730-40 pubmed
    ..Repair of this double-strand break (DSB) by homologous recombination may be responsible for the elevated levels of sister chromatid exchange (SCE) found in BLM(-/-) cells. ..
  20. Panizza S, Mendoza M, Berlinger M, Huang L, Nicolas A, Shirahige K, et al. Spo11-accessory proteins link double-strand break sites to the chromosome axis in early meiotic recombination. Cell. 2011;146:372-83 pubmed publisher
    ..homologous chromosomes initiates via programmed DNA double-strand breaks (DSBs), generated by complexes comprising Spo11 transesterase plus accessory proteins...
  21. Diaz R, Alcid A, Berger J, Keeney S. Identification of residues in yeast Spo11p critical for meiotic DNA double-strand break formation. Mol Cell Biol. 2002;22:1106-15 pubmed
    Saccharomyces cerevisiae Spo11 protein (Spo11p) is thought to generate the DNA double-strand breaks (DSBs) that initiate homologous recombination during meiosis...
  22. Neale M, Pan J, Keeney S. Endonucleolytic processing of covalent protein-linked DNA double-strand breaks. Nature. 2005;436:1053-7 pubmed
    DNA double-strand breaks (DSBs) with protein covalently attached to 5' strand termini are formed by Spo11 to initiate meiotic recombination...
  23. Penkner A, Prinz S, Ferscha S, Klein F. Mnd2, an essential antagonist of the anaphase-promoting complex during meiotic prophase. Cell. 2005;120:789-801 pubmed
    ..Human oocytes may spend many years in meiotic prophase before maturation. Inhibitors of meiotic APC variants could prevent loss of chiasmata also in these cells, thereby guarding against aberrant chromosome segregation. ..
  24. Falk J, Chan A, Hoffmann E, Hochwagen A. A Mec1- and PP4-dependent checkpoint couples centromere pairing to meiotic recombination. Dev Cell. 2010;19:599-611 pubmed publisher
  25. Zanders S, Alani E. The pch2Delta mutation in baker's yeast alters meiotic crossover levels and confers a defect in crossover interference. PLoS Genet. 2009;5:e1000571 pubmed publisher
    ..Lastly, the pch2Delta mutation decreases spore viability and disrupts crossover interference in spo11 hypomorph strains that have reduced levels of meiosis-induced double-strand breaks...
  26. Arora C, Kee K, Maleki S, Keeney S. Antiviral protein Ski8 is a direct partner of Spo11 in meiotic DNA break formation, independent of its cytoplasmic role in RNA metabolism. Mol Cell. 2004;13:549-59 pubmed
    Meiotic recombination initiates with double-strand breaks (DSBs) catalyzed by Spo11 in conjunction with accessory proteins whose roles are not understood...
  27. Neale M, Keeney S. End-labeling and analysis of Spo11-oligonucleotide complexes in Saccharomyces cerevisiae. Methods Mol Biol. 2009;557:183-95 pubmed publisher
    During meiosis Spo11 catalyzes the formation of DNA double-strand breaks, becoming covalently attached to the 5' ends on both sides of the break during this process...
  28. Peoples T, Dean E, Gonzalez O, Lambourne L, Burgess S. Close, stable homolog juxtaposition during meiosis in budding yeast is dependent on meiotic recombination, occurs independently of synapsis, and is distinct from DSB-independent pairing contacts. Genes Dev. 2002;16:1682-95 pubmed
    ..These data suggest that stable, close homolog juxtaposition in yeast is distinct from pre-DSB pairing interactions, requires both DSB and SEI formation, but does not depend on crossovers or SC. ..
  29. Carballo J, Panizza S, Serrentino M, Johnson A, Geymonat M, Borde V, et al. Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery. PLoS Genet. 2013;9:e1003545 pubmed publisher
    An essential feature of meiosis is Spo11 catalysis of programmed DNA double strand breaks (DSBs)...
  30. Kateneva A, Konovchenko A, Guacci V, Dresser M. Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae. J Cell Biol. 2005;171:241-53 pubmed
    ..We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes. ..
  31. Komakhin R, Komakhina V. [Compartmentalization of Spo11p in vegetative cells of yeast Saccharomyces cerevisiae]. Mol Biol (Mosk). 2008;42:494-500 pubmed
    ..Nineteen N-terminal amino acids of Spo11p fused to EGFP made some reporter to be localized in the nucleus...
  32. McKee A, Kleckner N. Mutations in Saccharomyces cerevisiae that block meiotic prophase chromosome metabolism and confer cell cycle arrest at pachytene identify two new meiosis-specific genes SAE1 and SAE3. Genetics. 1997;146:817-34 pubmed
    ..e., spo11. We show that sae1-1 and sae3-1 mutations each confer a distinct defect in meiotic recombination...
  33. Arbel Eden A, Joseph Strauss D, Masika H, Printzental O, Rachi E, Simchen G. Trans-Lesion DNA Polymerases May Be Involved in Yeast Meiosis. G3 (Bethesda). 2013;3:633-644 pubmed publisher
    ..significant yeast two-hybrid (Y2H) associations in meiosis-arrested cells between the TLSPs and DSB proteins Rev1-Spo11, Rev1-Mei4, and Rev7-Rec114, as well as between Rev1 and Rad30 We suggest that the involvement of TLSPs in ..
  34. Blitzblau H, Hochwagen A. ATR/Mec1 prevents lethal meiotic recombination initiation on partially replicated chromosomes in budding yeast. elife. 2013;2:e00844 pubmed publisher
    ..by Dbf4-dependent Cdc7 kinase, preclusion of chromosomal loading of Rec114 and Mre11, and lowered abundance of the Spo11 nuclease. Without this checkpoint, cells formed DSBs on partially replicated chromosomes...
  35. Dayani Y, Simchen G, Lichten M. Meiotic recombination intermediates are resolved with minimal crossover formation during return-to-growth, an analogue of the mitotic cell cycle. PLoS Genet. 2011;7:e1002083 pubmed publisher
    ..Thus, in contrast to meiosis, where JM resolution is heavily biased towards COs, JM resolution during RTG minimizes CO formation, thus maintaining genome integrity and minimizing loss of heterozygosity. ..
  36. Humphryes N, Leung W, Argunhan B, Terentyev Y, Dvorácková M, Tsubouchi H. The Ecm11-Gmc2 complex promotes synaptonemal complex formation through assembly of transverse filaments in budding yeast. PLoS Genet. 2013;9:e1003194 pubmed publisher
    ..We propose that the Ecm11-Gmc2 complex facilitates the assembly of Zip1 and that SUMOylation of Ecm11 is critical for ensuring chromosomal assembly of Zip1, thus suppressing polycomplex formation...
  37. Kamieniecki R, Shanks R, Dawson D. Slk19p is necessary to prevent separation of sister chromatids in meiosis I. Curr Biol. 2000;10:1182-90 pubmed
    ..Our results suggest that Slk19p is essential at the centromere of meiotic chromosomes to prevent the premature separation of sister chromatids at meiosis I. ..
  38. Mohibullah N, Keeney S. Numerical and spatial patterning of yeast meiotic DNA breaks by Tel1. Genome Res. 2017;27:278-288 pubmed publisher
    The Spo11-generated double-strand breaks (DSBs) that initiate meiotic recombination are dangerous lesions that can disrupt genome integrity, so meiotic cells regulate their number, timing, and distribution...
  39. Nolt J, Rice L, Gallo Ebert C, Bisher M, Nickels J. PP2A (Cdc)?? is required for multiple events during meiosis I. Cell Cycle. 2011;10:1420-34 pubmed
    ..We conclude PP2A (Cdc)?? is required for the proper temporal initiation of multiple meiotic events and/or monitors these events to ensure their fidelity. ..
  40. Rice L, Plakas C, Nickels J. Loss of meiotic rereplication block in Saccharomyces cerevisiae cells defective in Cdc28p regulation. Eukaryot Cell. 2005;4:55-62 pubmed
    ..Finally, we present evidence of a possible role for a Cdc55p-dependent protein phosphatase 2A in initiating meiotic replication. ..
  41. Lao J, Cloud V, Huang C, Grubb J, Thacker D, Lee C, et al. Meiotic crossover control by concerted action of Rad51-Dmc1 in homolog template bias and robust homeostatic regulation. PLoS Genet. 2013;9:e1003978 pubmed publisher
    ..Thus, robust crossover homeostasis is conferred by integrated regulation at initiation, strand-exchange and maturation steps of meiotic recombination...
  42. Li P, Shao Y, Jin H, Yu H. Ndj1, a telomere-associated protein, regulates centrosome separation in budding yeast meiosis. J Cell Biol. 2015;209:247-59 pubmed publisher
    ..These findings reveal the underlying mechanism that coordinates yeast centrosome dynamics with meiotic telomere movement and cell cycle progression. ..
  43. Chu D, Gromova T, Newman T, Burgess S. The Nucleoporin Nup2 Contains a Meiotic-Autonomous Region that Promotes the Dynamic Chromosome Events of Meiosis. Genetics. 2017;206:1319-1337 pubmed publisher
    ..These data suggest Nup2 and Ndj1 support partially overlapping functions that promote two different levels of meiotic chromosome organization necessary to withstand a dynamic stage of the eukaryotic life cycle. ..
  44. Nerusheva O, Galander S, Fernius J, Kelly D, Marston A. Tension-dependent removal of pericentromeric shugoshin is an indicator of sister chromosome biorientation. Genes Dev. 2014;28:1291-309 pubmed publisher
    ..Disassembly of this platform in response to intersister kinetochore tension signals the bioriented state. Therefore, tension sensing by shugoshin is a central mechanism by which the bioriented state is read. ..
  45. Cooper K, Mallory M, Guacci V, Lowe K, Strich R. Pds1p is required for meiotic recombination and prophase I progression in Saccharomyces cerevisiae. Genetics. 2009;181:65-79 pubmed publisher
    ..This role is meiosis specific as Mcd1p destruction is not altered in vegetative pds1Delta cultures. These results define a previously undescribed role for Pds1p in cohesin maintenance, recombination, and meiotic progression. ..
  46. Usui T, Kanehara Y. Elevated Rad53 kinase activity influences formation and interhomolog repair of meiotic DNA double-strand breaks in budding yeast. Biochem Biophys Res Commun. 2013;441:593-9 pubmed publisher
    ..The overexpression led to partial activation of Rad53, uncovering that the negative impacts of Rad53 kinase activation on meiotic progression, and formation and interhomolog repair of meiotic programmed DSBs. ..
  47. Obeso D, Dawson D. Temporal characterization of homology-independent centromere coupling in meiotic prophase. PLoS ONE. 2010;5:e10336 pubmed publisher
    ..Finally, the timely release of centromere coupling depends on the recombination-initiating enzyme, Spo11, suggesting a connection between events in homologous pairing/recombination and the regulation of centromere ..
  48. Lin W, Jin H, Liu X, Hampton K, Yu H. Scc2 regulates gene expression by recruiting cohesin to the chromosome as a transcriptional activator during yeast meiosis. Mol Biol Cell. 2011;22:1985-96 pubmed publisher
    ..Finally, we provide evidence that chromosomal binding of cohesin is sufficient for target-gene activation during meiosis. Our data support a noncanonical role for cohesin as a transcriptional activator during cell differentiation. ..
  49. Katis V, Lipp J, Imre R, Bogdanova A, Okaz E, Habermann B, et al. Rec8 phosphorylation by casein kinase 1 and Cdc7-Dbf4 kinase regulates cohesin cleavage by separase during meiosis. Dev Cell. 2010;18:397-409 pubmed publisher
    ..Our data suggest that PP2A protects centromeric cohesion by opposing CK1delta/epsilon- and DDK-dependent phosphorylation of Rec8. ..
  50. Lee C, Conrad M, Dresser M. Meiotic chromosome pairing is promoted by telomere-led chromosome movements independent of bouquet formation. PLoS Genet. 2012;8:e1002730 pubmed publisher
  51. Wagstaff J, Klapholz S, Esposito R. Meiosis in haploid yeast. Proc Natl Acad Sci U S A. 1982;79:2986-90 pubmed
    ..Recombination in spo13-1 haploids is blocked by the spo11-1 mutation, which also eliminates recombination between homologous chromosomes during conventional diploid meiosis...
  52. Rockmill B, Lefrançois P, Voelkel Meiman K, Oke A, Roeder G, Fung J. High throughput sequencing reveals alterations in the recombination signatures with diminishing Spo11 activity. PLoS Genet. 2013;9:e1003932 pubmed publisher
    b>Spo11 is the topoisomerase-like enzyme responsible for the induction of the meiosis-specific double strand breaks (DSBs), which initiates the recombination events responsible for proper chromosome segregation...
  53. Fukuda T, Kugou K, Sasanuma H, Shibata T, Ohta K. Targeted induction of meiotic double-strand breaks reveals chromosomal domain-dependent regulation of Spo11 and interactions among potential sites of meiotic recombination. Nucleic Acids Res. 2008;36:984-97 pubmed
    Meiotic recombination is initiated by programmed DNA double-strand break (DSB) formation mediated by Spo11. DSBs occur with frequency in chromosomal regions called hot domains but are seldom seen in cold domains...
  54. Vincenten N, Kuhl L, Lam I, Oke A, Kerr A, Hochwagen A, et al. The kinetochore prevents centromere-proximal crossover recombination during meiosis. elife. 2015;4: pubmed publisher
  55. Kugou K, Fukuda T, Yamada S, Ito M, Sasanuma H, Mori S, et al. Rec8 guides canonical Spo11 distribution along yeast meiotic chromosomes. Mol Biol Cell. 2009;20:3064-76 pubmed publisher
    b>Spo11-mediated DNA double-strand breaks (DSBs) that initiate meiotic recombination are temporally and spatially controlled...
  56. Sarno R, Vicq Y, Uematsu N, Luka M, Lapierre C, Carroll D, et al. Programming sites of meiotic crossovers using Spo11 fusion proteins. Nucleic Acids Res. 2017;45:e164 pubmed publisher
    ..we report the local stimulation of meiotic recombination at a number of chromosomal sites by tethering the natural Spo11 protein to various DNA-binding modules: full-length DNA binding proteins, zinc fingers (ZFs), transcription ..
  57. Kiburz B, Amon A, Marston A. Shugoshin promotes sister kinetochore biorientation in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:1199-209 pubmed
    ..In contrast, Sgo1 is important to bias sister kinetochores toward biorientation. We suggest that Sgo1 acts at sister kinetochores to promote their biorientation. ..
  58. Nishant K, Chen C, Shinohara M, Shinohara A, Alani E. Genetic analysis of baker's yeast Msh4-Msh5 reveals a threshold crossover level for meiotic viability. PLoS Genet. 2010;6: pubmed publisher
    ..A triple mutant containing a msh4/5-t allele and mutations that decreased meiotic double-strand break levels (spo11-HA) and crossover interference (pch2?) showed synergistic defects in spore viability...
  59. Zhu X, Keeney S. High-Resolution Global Analysis of the Influences of Bas1 and Ino4 Transcription Factors on Meiotic DNA Break Distributions in Saccharomyces cerevisiae. Genetics. 2015;201:525-42 pubmed publisher
    Meiotic recombination initiates with DNA double-strand breaks (DSBs) made by Spo11. In Saccharomyces cerevisiae, many DSBs occur in "hotspots" coinciding with nucleosome-depleted gene promoters...
  60. Lefrançois P, Rockmill B, Xie P, Roeder G, Snyder M. Multiple Pairwise Analysis of Non-homologous Centromere Coupling Reveals Preferential Chromosome Size-Dependent Interactions and a Role for Bouquet Formation in Establishing the Interaction Pattern. PLoS Genet. 2016;12:e1006347 pubmed publisher
    ..initiation of recombination and the presence of homologs are dispensable for centromere coupling (occurring in spo11 mutants and haploids induced to undergo meiosis) but the presence of the synaptonemal complex (SC) protein Zip1 is ..
  61. Cheng Z, Liu Y, Wang C, Parker R, Song H. Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination. Protein Sci. 2004;13:2673-84 pubmed
    ..In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution...