Gene Symbol: Spf1p
Description: ion-transporting P-type ATPase SPF1
Alias: COD1, PER9, PIO1, ion-transporting P-type ATPase SPF1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Suzuki C. Immunochemical and mutational analyses of P-type ATPase Spf1p involved in the yeast secretory pathway. Biosci Biotechnol Biochem. 2001;65:2405-11 pubmed
    ..To investigate the function of Spf1p, Asp487, the putative phosphorylation site of Spf1p, was replaced by Asn...
  2. Tipper D, Harley C. Yeast genes controlling responses to topogenic signals in a model transmembrane protein. Mol Biol Cell. 2002;13:1158-74 pubmed
    ..Pmr1p is the Golgi Ca(2+) ATPase and Spf1p may be the equivalent ER pump. PIO2 is STE24, a metalloprotease anchored in the ER membrane...
  3. Cronin S, Rao R, Hampton R. Cod1p/Spf1p is a P-type ATPase involved in ER function and Ca2+ homeostasis. J Cell Biol. 2002;157:1017-28 pubmed
    ..Cod1p/Spf1p belongs to the widely conserved, uncharacterized type V branch of P-type ATPases, a large family of ion pumps...
  4. Ando A, Suzuki C. Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae. Mol Genet Genomics. 2005;273:497-506 pubmed
    b>Spf1p is a P-type ATPase that is mainly localized to the endoplasmic reticulum (ER) in Saccharomyces cerevisiae. The protein is involved in the maintenance of ion homeostasis in the ER...
  5. Corradi G, de Tezanos Pinto F, Mazzitelli L, Adamo H. Shadows of an absent partner: ATP hydrolysis and phosphoenzyme turnover of the Spf1 (sensitivity to Pichia farinosa killer toxin) P5-ATPase. J Biol Chem. 2012;287:30477-84 pubmed publisher
    ..This behavior may reflect a limiting countertransported substrate needed to promote turnover or a missing regulatory factor...
  6. Vashist S, Frank C, Jakob C, Ng D. Two distinctly localized p-type ATPases collaborate to maintain organelle homeostasis required for glycoprotein processing and quality control. Mol Biol Cell. 2002;13:3955-66 pubmed
    ..Here, we report the identification of COD1/SPF1 (control of HMG-CoA reductase degradation/SPF1) through genetic strategies intended to uncover genes involved ..
  7. Gitler A, Chesi A, Geddie M, Strathearn K, Hamamichi S, Hill K, et al. Alpha-synuclein is part of a diverse and highly conserved interaction network that includes PARK9 and manganese toxicity. Nat Genet. 2009;41:308-15 pubmed publisher
    ..Finally, we show that additional genes from our yeast screen, with diverse functions, are potent modifiers of alpha-syn-induced neuron loss in animals, establishing a diverse, highly conserved interaction network for alpha-syn. ..
  8. Krumpe K, Frumkin I, Herzig Y, Rimon N, Ozbalci C, Brugger B, et al. Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes. Mol Biol Cell. 2012;23:3927-35 pubmed publisher
    ..We therefore suggest that the inherent differences in membrane composition between organelle membranes are sufficient to determine membrane integration specificity in a eukaryotic cell. ..
  9. Cohen Y, Megyeri M, Chen O, Condomitti G, Riezman I, Loizides Mangold U, et al. The yeast p5 type ATPase, spf1, regulates manganese transport into the endoplasmic reticulum. PLoS ONE. 2013;8:e85519 pubmed publisher
    ..Conversely, cytosolic Mn(2+)-dependent processes were increased. Together, these data support a role for Spf1p in Mn(2+) transport in the cell...

More Information


  1. Takahashi H, McCaffery J, Irizarry R, Boeke J. Nucleocytosolic acetyl-coenzyme a synthetase is required for histone acetylation and global transcription. Mol Cell. 2006;23:207-17 pubmed
    ..Thus, acetyl-CoA metabolism is directly linked to chromatin regulation and may affect diverse cellular processes in which acetylation and metabolism intersect, such as disease states and aging. ..
  2. Puigpinós J, Casas C, Herrero E. Altered intracellular calcium homeostasis and endoplasmic reticulum redox state in Saccharomyces cerevisiae cells lacking Grx6 glutaredoxin. Mol Biol Cell. 2015;26:104-16 pubmed publisher
    ..The results presented in this study provide evidence for redox regulation of calcium homeostasis in yeast cells. ..
  3. Heeren G, Rinnerthaler M, Laun P, von Seyerl P, Kössler S, Klinger H, et al. The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1. Aging (Albany NY). 2009;1:622-36 pubmed
    ..AFO1/MRPL25 function establishes a new connection between mitochondria, metabolism and aging. ..
  4. Lauffer S, Mäbert K, Czupalla C, Pursche T, Hoflack B, Rödel G, et al. Saccharomyces cerevisiae porin pore forms complexes with mitochondrial outer membrane proteins Om14p and Om45p. J Biol Chem. 2012;287:17447-58 pubmed publisher
    ..We propose a model for the role of the two OM proteins in addressing the porin pore to bind to specific channels in the mt IM to facilitate transport of metabolites. ..
  5. Sørensen D, Holen H, Holemans T, Vangheluwe P, Palmgren M. Towards defining the substrate of orphan P5A-ATPases. Biochim Biophys Acta. 2015;1850:524-35 pubmed publisher
    ..P5A-ATPases constitute a poorly characterized subfamily of P-type ATPases present in all eukaryotic organisms but for which a transported substrate remains to be identified...
  6. Corradi G, Czysezon N, Mazzitelli L, Sarbia N, Adamo H. Inhibition of the Formation of the Spf1p Phosphoenzyme by Ca2. J Biol Chem. 2016;291:7767-73 pubmed publisher
    ..In the yeastSaccharomyces cerevisiae, deletion of P5-ATPase Spf1p gives rise to endoplasmic reticulum stress. The reaction cycle of P5-ATPases is poorly characterized...