SOD1

Summary

Gene Symbol: SOD1
Description: superoxide dismutase SOD1
Alias: CRS4, superoxide dismutase SOD1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Harris N, Bachler M, Costa V, Mollapour M, Moradas Ferreira P, Piper P. Overexpressed Sod1p acts either to reduce or to increase the lifespans and stress resistance of yeast, depending on whether it is Cu(2+)-deficient or an active Cu,Zn-superoxide dismutase. Aging Cell. 2005;4:41-52 pubmed
    Yeast overexpressing SOD1, the gene for Cu,Zn-superoxide dismutase (Cu,Zn-Sod), was used to determine how Sod1p overexpression influences the chronological lifespan [the survival of non-dividing stationary (G0) phase cells over time], the ..
  2. Sturtz L, Diekert K, Jensen L, Lill R, Culotta V. A fraction of yeast Cu,Zn-superoxide dismutase and its metallochaperone, CCS, localize to the intermembrane space of mitochondria. A physiological role for SOD1 in guarding against mitochondrial oxidative damage. J Biol Chem. 2001;276:38084-9 pubmed
    Cu,Zn-superoxide dismutase (SOD1) is an abundant, largely cytosolic enzyme that scavenges superoxide anions...
  3. Liu X, Elashvili I, Gralla E, Valentine J, Lapinskas P, Culotta V. Yeast lacking superoxide dismutase. Isolation of genetic suppressors. J Biol Chem. 1992;267:18298-302 pubmed
    ..In addition to being hypersensitive to oxygen toxicity, strains containing deletions in both the SOD1 (encoding Cu/Zn-SOD) and SOD2 (encoding Mn-SOD) genes are defective in sporulation, are associated with a high ..
  4. Bermingham McDonogh O, Gralla E, Valentine J. The copper, zinc-superoxide dismutase gene of Saccharomyces cerevisiae: cloning, sequencing, and biological activity. Proc Natl Acad Sci U S A. 1988;85:4789-93 pubmed
    ..The role of Cu,Zn-SOD in defense against dioxygen toxicity is discussed in the light of these findings. ..
  5. Klöppel C, Michels C, Zimmer J, Herrmann J, Riemer J. In yeast redistribution of Sod1 to the mitochondrial intermembrane space provides protection against respiration derived oxidative stress. Biochem Biophys Res Commun. 2010;403:114-9 pubmed publisher
    The antioxidative enzyme copper-zinc superoxide dismutase (Sod1) is an important cellular defence system against reactive oxygen species (ROS)...
  6. Neklesa T, Davis R. Superoxide anions regulate TORC1 and its ability to bind Fpr1:rapamycin complex. Proc Natl Acad Sci U S A. 2008;105:15166-71 pubmed publisher
    ..Among these were Cu/Zn-superoxide dismutase Sod1, copper transporter Ctr1, and copper chaperone Lys7, suggesting a role for oxidative stress in rapamycin ..
  7. Longo V, Gralla E, Valentine J. Superoxide dismutase activity is essential for stationary phase survival in Saccharomyces cerevisiae. Mitochondrial production of toxic oxygen species in vivo. J Biol Chem. 1996;271:12275-80 pubmed
    ..Our results suggest strongly that mitochondrial respiration is a major source of reactive oxygen species in vivo, as has been shown in vitro, and that these species are produced even under low aeration. ..
  8. Wei J, Srinivasan C, Han H, Valentine J, Gralla E. Evidence for a novel role of copper-zinc superoxide dismutase in zinc metabolism. J Biol Chem. 2001;276:44798-803 pubmed
    ..yCCS) that delivers copper to the active site of copper-zinc superoxide dismutase (CuZn-SOD, a product of the SOD1 gene). In yeast lacking Lys7 (lys7Delta), the SOD1 polypeptide is present but inactive...
  9. Tan S, Teo M, Lam Y, Dawes I, Perrone G. Cu, Zn superoxide dismutase and NADP(H) homeostasis are required for tolerance of endoplasmic reticulum stress in Saccharomyces cerevisiae. Mol Biol Cell. 2009;20:1493-508 pubmed publisher
    ..by dithiothreitol and tunicamycin (TM) identified mutants deleted for Cu, Zn superoxide dismutase (SOD) function (SOD1, CCS1) or affected in NADPH generation via the pentose phosphate pathway (TKL1, RPE1)...

More Information

Publications69

  1. Park J, Grant C, Davies M, Dawes I. The cytoplasmic Cu,Zn superoxide dismutase of saccharomyces cerevisiae is required for resistance to freeze-thaw stress. Generation of free radicals during freezing and thawing. J Biol Chem. 1998;273:22921-8 pubmed
    ..using mutants defective in a range of antioxidant functions, including Cu,Zn superoxide dismutase (encoded by SOD1), Mn superoxide dismutase (SOD2), catalase A, catalase T, glutathione reductase, gamma-glutamylcysteine synthetase ..
  2. Carter C, Kitchen L, Au W, Babic C, Basrai M. Loss of SOD1 and LYS7 sensitizes Saccharomyces cerevisiae to hydroxyurea and DNA damage agents and downregulates MEC1 pathway effectors. Mol Cell Biol. 2005;25:10273-85 pubmed
    ..We show that loss of the Cu,Zn-dependent superoxide dismutase, SOD1, or its copper chaperone, LYS7, confers oxygen-dependent sensitivity to replication arrest and DNA damage in ..
  3. Liu X, Zhang X, Zhang Z. Cu,Zn-superoxide dismutase is required for cell wall structure and for tolerance to cell wall-perturbing agents in Saccharomyces cerevisiae. FEBS Lett. 2010;584:1245-50 pubmed publisher
    Here we report that deletion of SOD1, the Cu,Zn-superoxide dismutase in Saccharomyces cerevisiae is sensitive to cell wall-perturbing agents, such as Calcofluor white and Congo red...
  4. Reddi A, Culotta V. Regulation of manganese antioxidants by nutrient sensing pathways in Saccharomyces cerevisiae. Genetics. 2011;189:1261-70 pubmed publisher
    ..Our observed roles for nutrient and stress response kinases and transcription factors in regulating the Mn antioxidant underscore its physiological importance in aerobic fitness. ..
  5. Reddi A, Jensen L, Naranuntarat A, Rosenfeld L, Leung E, Shah R, et al. The overlapping roles of manganese and Cu/Zn SOD in oxidative stress protection. Free Radic Biol Med. 2009;46:154-62 pubmed publisher
    ..cerevisiae to analyze factors that promote manganese as an antioxidant in cells lacking Cu/Zn superoxide dismutase (sod1 Delta)...
  6. Favre C, Aguilar P, Carrillo M. Oxidative stress and chronological aging in glycogen-phosphorylase-deleted yeast. Free Radic Biol Med. 2008;45:1446-56 pubmed publisher
    ..Extra copies of SOD1/2 counteracted the stress sensitivity and the accelerated aging of gph1delta...
  7. Sea K, Sheng Y, Lelie H, Kane Barnese L, Durazo A, Valentine J, et al. Yeast copper-zinc superoxide dismutase can be activated in the absence of its copper chaperone. J Biol Inorg Chem. 2013;18:985-92 pubmed publisher
    Copper-zinc superoxide dismutase (Sod1) is an abundant intracellular enzyme that catalyzes the disproportionation of superoxide to give hydrogen peroxide and dioxygen...
  8. Tsang C, Liu Y, Thomas J, Zhang Y, Zheng X. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance. Nat Commun. 2014;5:3446 pubmed publisher
    Superoxide dismutase 1 (Sod1) has been known for nearly half a century for catalysis of superoxide to hydrogen peroxide...
  9. Madia F, Wei M, Yuan V, Hu J, Gattazzo C, Pham P, et al. Oncogene homologue Sch9 promotes age-dependent mutations by a superoxide and Rev1/Polzeta-dependent mechanism. J Cell Biol. 2009;186:509-23 pubmed publisher
    ..Deletion of REV1, the lack of components of the error-prone Polzeta, or the overexpression of SOD1 or SOD2 is sufficient to reduce age-dependent point mutations in SCH9 overexpressors, but REV1 deficiency causes a ..
  10. Leitch J, Jensen L, Bouldin S, Outten C, Hart P, Culotta V. Activation of Cu,Zn-superoxide dismutase in the absence of oxygen and the copper chaperone CCS. J Biol Chem. 2009;284:21863-71 pubmed publisher
    ..To investigate SOD1 activation in the absence of CCS, we compared and contrasted the CCS-independent activation of C...
  11. Baron J, Chen J, Culotta V. Cu/Zn superoxide dismutase and the proton ATPase Pma1p of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2015;462:251-6 pubmed publisher
    In eukaryotes, the Cu/Zn containing superoxide dismutase (SOD1) plays a critical role in oxidative stress protection as well as in signaling...
  12. Hlavatá L, Nachin L, Jezek P, Nystrom T. Elevated Ras/protein kinase A activity in Saccharomyces cerevisiae reduces proliferation rate and lifespan by two different reactive oxygen species-dependent routes. Aging Cell. 2008;7:148-57 pubmed
    ..While the reduction in generation time is linked to the inactivation, specifically, of the mitochondrial nucleotide carrier, longevity is affected by other, and hitherto unknown, target(s) of ROS attack. ..
  13. Jensen L, Sanchez R, Srinivasan C, Valentine J, Culotta V. Mutations in Saccharomyces cerevisiae iron-sulfur cluster assembly genes and oxidative stress relevant to Cu,Zn superoxide dismutase. J Biol Chem. 2004;279:29938-43 pubmed
    Saccharomyces cerevisiae lacking Cu,Zn superoxide dismutase (SOD1) show several metabolic defects including aerobic blockages in methionine and lysine biosynthesis...
  14. Karachitos A, Galganska H, Wojtkowska M, Budzinska M, Stobienia O, Bartosz G, et al. Cu,Zn-superoxide dismutase is necessary for proper function of VDAC in Saccharomyces cerevisiae cells. FEBS Lett. 2009;583:449-55 pubmed publisher
    ..The observed alterations may result in mitochondriopathy and subsequently in the shortening of the replicative life span observed for S. cerevisiaeDeltasod1 cells. ..
  15. Sun C, Zhou B. The antimalarial drug artemisinin induces an additional, Sod1-supressible anti-mitochondrial action in yeast. Biochim Biophys Acta Mol Cell Res. 2017;1864:1285-1294 pubmed publisher
    ..that the lack of toxicity of ART even at high levels (200-400?M) on fermentable medium is due to the presence of Sod1. While we expected this normally Sod1-supressed action to be heme-mediated like DHA, surprisingly, this toxicity of ..
  16. Culotta V, Joh H, Lin S, Slekar K, Strain J. A physiological role for Saccharomyces cerevisiae copper/zinc superoxide dismutase in copper buffering. J Biol Chem. 1995;270:29991-7 pubmed
    ..We now demonstrate that CRS4 is equivalent to SOD1, encoding copper/zinc superoxide dismutase (SOD)...
  17. Lamb A, Torres A, O Halloran T, Rosenzweig A. Heterodimeric structure of superoxide dismutase in complex with its metallochaperone. Nat Struct Biol. 2001;8:751-5 pubmed
    ..for superoxide dismutase (CCS) activates the eukaryotic antioxidant enzyme copper, zinc superoxide dismutase (SOD1). The 2...
  18. Schmidt P, Kunst C, Culotta V. Copper activation of superoxide dismutase 1 (SOD1) in vivo. Role for protein-protein interactions with the copper chaperone for SOD1. J Biol Chem. 2000;275:33771-6 pubmed
    Insertion of copper into superoxide dismutase 1 (SOD1) in vivo requires the copper chaperone for SOD1 (CCS)...
  19. Srinivasan C, Liba A, Imlay J, Valentine J, Gralla E. Yeast lacking superoxide dismutase(s) show elevated levels of "free iron" as measured by whole cell electron paramagnetic resonance. J Biol Chem. 2000;275:29187-92 pubmed
    ..strain, whereas significantly elevated levels were found in strains lacking CuZn-superoxide dismutase (CuZn-SOD) (sod1 delta), Mn-SOD (sod2 delta), or both SODs, throughout their growth but particularly in stationary phase...
  20. Pope C, De Feo C, Unger V. Cellular distribution of copper to superoxide dismutase involves scaffolding by membranes. Proc Natl Acad Sci U S A. 2013;110:20491-6 pubmed publisher
    ..Here we demonstrate that the human and yeast copper chaperones (CCS) for superoxide dismutase 1 (SOD1), long thought to exclusively reside in the cytosol and mitochondrial intermembrane space, can engage negatively ..
  21. Liu X, Supek F, Nelson N, Culotta V. Negative control of heavy metal uptake by the Saccharomyces cerevisiae BSD2 gene. J Biol Chem. 1997;272:11763-9 pubmed
    ..Instead, this cobalt accumulation was found to be specifically mediated through the SMF1 homologue, SMF2. Hence, BSD2 prevents metal hyperaccumulation by exerting negative control over the SMF1 and SMF2 metal transport systems. ..
  22. Lapinskas P, Cunningham K, Liu X, Fink G, Culotta V. Mutations in PMR1 suppress oxidative damage in yeast cells lacking superoxide dismutase. Mol Cell Biol. 1995;15:1382-8 pubmed
    Mutants of Saccharomyces cerevisiae lacking a functional SOD1 gene encoding Cu/Zn superoxide dismutase (SOD) are sensitive to atmospheric levels of oxygen and are auxotrophic for lysine and methionine when grown in air...
  23. Lushchak O, Nykorak N, Ohdate T, Inoue Y, Lushchak V. Inactivation of genes encoding superoxide dismutase modifies yeast response to S-nitrosoglutathione-induced stress. Biochemistry (Mosc). 2009;74:445-51 pubmed
    ..A GSNO-induced increase in oxidized glutathione level in the studied yeast strains might account for cell killing by GSNO due to the development of oxidative/nitrosative stress. ..
  24. Fetherolf M, Boyd S, Taylor A, Kim H, Wohlschlegel J, Blackburn N, et al. Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site. J Biol Chem. 2017;292:12025-12040 pubmed publisher
    ..The copper chaperone for superoxide dismutase (Ccs1) activates immature copper-zinc superoxide dismutase (Sod1) by delivering copper and facilitating the oxidation of the Sod1 intramolecular disulfide bond...
  25. Djinovic K, Gatti G, Coda A, Antolini L, Pelosi G, Desideri A, et al. Structure solution and molecular dynamics refinement of the yeast Cu,Zn enzyme superoxide dismutase. Acta Crystallogr B. 1991;47 ( Pt 6):918-27 pubmed
    ..The beta-barrel structure in the yeast enzyme is closed at the upper part by an efficient hydrogen-bonding scheme. ..
  26. Balasundaram D, Tabor C, Tabor H. Oxygen toxicity in a polyamine-depleted spe2 delta mutant of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1993;90:4693-7 pubmed
    ..However, since the mutant cells still required spermidine or spermine for growth when incubated under strictly anaerobic conditions, polyamines must also have other essential functions. ..
  27. De Freitas J, Liba A, Meneghini R, Valentine J, Gralla E. Yeast lacking Cu-Zn superoxide dismutase show altered iron homeostasis. Role of oxidative stress in iron metabolism. J Biol Chem. 2000;275:11645-9 pubmed
    Saccharomyces cerevisiae lacking copper-zinc superoxide dismutase (sod1) shows a series of defects, including reduced rates of aerobic growth in synthetic glucose medium and reduced ability to grow by respiration in glycerol-rich medium...
  28. Milgrom E, Diab H, Middleton F, Kane P. Loss of vacuolar proton-translocating ATPase activity in yeast results in chronic oxidative stress. J Biol Chem. 2007;282:7125-36 pubmed
    ..The results indicate that V-ATPase activity helps to protect cells from endogenous oxidative stress. ..
  29. Manfredini V, Duarte Martins V, Ruaro Peralba M, Silveira Benfato M. Adaptative response to enhanced basal oxidative damage in sod mutants from Saccharomyces cerevisiae. Mol Cell Biochem. 2005;276:175-81 pubmed
    ..Thus, both direct and indirect GSH pathways are involved in the protection of lipid membranes and proteins in these mutants and may constitute an adaptative response to enhanced basal oxidative damage produced by superoxide. ..
  30. O Brien K, Dirmeier R, Engle M, Poyton R. Mitochondrial protein oxidation in yeast mutants lacking manganese-(MnSOD) or copper- and zinc-containing superoxide dismutase (CuZnSOD): evidence that MnSOD and CuZnSOD have both unique and overlapping functions in protecting mitochondrial proteins . J Biol Chem. 2004;279:51817-27 pubmed
    ..dismutase (SOD): MnSOD, encoded by SOD2, which is located within the mitochondrial matrix, and CuZnSOD, encoded by SOD1, which is located in both the cytosol and the mitochondrial intermembrane space...
  31. Reddi A, Culotta V. SOD1 integrates signals from oxygen and glucose to repress respiration. Cell. 2013;152:224-35 pubmed publisher
    Cu/Zn superoxide dismutase (SOD1) is an abundant enzyme that has been best studied as a regulator of antioxidant defense...
  32. Wu C, Steffen J, Eide D. Cytosolic superoxide dismutase (SOD1) is critical for tolerating the oxidative stress of zinc deficiency in yeast. PLoS ONE. 2009;4:e7061 pubmed publisher
    ..Our results indicated that the cytosolic superoxide dismutase Sod1 is also critical for growth under zinc-limiting conditions...
  33. Strain J, Lorenz C, Bode J, Garland S, Smolen G, Ta D, et al. Suppressors of superoxide dismutase (SOD1) deficiency in Saccharomyces cerevisiae. Identification of proteins predicted to mediate iron-sulfur cluster assembly. J Biol Chem. 1998;273:31138-44 pubmed
    Yeast deficient in the cytosolic copper/zinc superoxide dismutase (SOD1) exhibit metabolic defects indicative of oxidative damage even under non-stress conditions...
  34. Roehrs R, Freitas D, Masuda A, Henriques J, Guecheva T, Ramos A, et al. Effect of vitamin A treatment on superoxide dismutase-deficient yeast strains. Arch Microbiol. 2010;192:221-8 pubmed publisher
    ..However, the induction of GPx activity is not sufficient to prevent the Vit A-induced cell death in these mutants in exponential phase growth. ..
  35. Mannarino S, Vilela L, Brasil A, Aranha J, Moradas Ferreira P, Pereira M, et al. Requirement of glutathione for Sod1 activation during lifespan extension. Yeast. 2011;28:19-25 pubmed publisher
    It has been shown that the activation of cytosolic superoxide dismutase (Sod1) in Saccharomyces cerevisiae is only dependent on Ccs1, which is responsible for insertion of copper into the enzyme catalytic center, and that glutathione (GSH)..
  36. Batova M, Klobucnikova V, Oblasova Z, Gregan J, Zahradnik P, Hapala I, et al. Chemogenomic and transcriptome analysis identifies mode of action of the chemosensitizing agent CTBT (7-chlorotetrazolo[5,1-c]benzo[1,2,4]triazine). BMC Genomics. 2010;11:153 pubmed publisher
    ..We conclude that CTBT causes intracellular superoxide production and oxidative stress in fungal cells and is thus enhancing antimycotic drug effects by a secondary stress. ..
  37. Rattanawong K, Kerdsomboon K, Auesukaree C. Cu/Zn-superoxide dismutase and glutathione are involved in response to oxidative stress induced by protein denaturing effect of alachlor in Saccharomyces cerevisiae. Free Radic Biol Med. 2015;89:963-71 pubmed publisher
    ..Although only SOD1 encoding Cu/Zn-superoxide dismutase (SOD), but not SOD2 encoding Mn-SOD, is essential for alachlor tolerance, both ..
  38. Nedd S, Redler R, Proctor E, Dokholyan N, Alexandrova A. Cu,Zn-superoxide dismutase without Zn is folded but catalytically inactive. J Mol Biol. 2014;426:4112-4124 pubmed publisher
    Amyotrophic lateral sclerosis has been linked to the gain of aberrant function of superoxide dismutase, Cu,Zn-SOD1 upon protein misfolding...
  39. Tang H, Siu K, Wong C, Jin D. Loss of yeast peroxiredoxin Tsa1p induces genome instability through activation of the DNA damage checkpoint and elevation of dNTP levels. PLoS Genet. 2009;5:e1000697 pubmed publisher
    ..Taken together, our findings uncover a new role of TSA1 in preventing the overproduction of dNTPs, which is a root cause of genome instability. ..
  40. Sheng Y, Butler Gralla E, Schumacher M, Cascio D, Cabelli D, Valentine J. Six-coordinate manganese(3+) in catalysis by yeast manganese superoxide dismutase. Proc Natl Acad Sci U S A. 2012;109:14314-9 pubmed publisher
    ..Because WT and the mutant yeast MnSOD both rest in the 2+ state and become six-coordinate when oxidized up from Mn(2+), six-coordinate Mn(3+) species could also actively function in the mechanism of WT yeast MnSODs. ..
  41. Devasahayam G, Burke D, Sturgill T. Golgi manganese transport is required for rapamycin signaling in Saccharomyces cerevisiae. Genetics. 2007;177:231-8 pubmed
    ..Overexpression of Ccc1, a Fe2+ and Mn2+ transporter that has been localized to Golgi and the vacuole, does restore rapamycin sensitivity to pmr1Delta. We conclude that Mn2+ in the Golgi inhibits TORC1 signaling. ..
  42. Sadowska Bartosz I, Pączka A, Mołoń M, Bartosz G. Dimethyl sulfoxide induces oxidative stress in the yeast Saccharomyces cerevisiae. FEMS Yeast Res. 2013;13:820-30 pubmed publisher
    ..Saccharomyces cerevisiae wt strain EG-103 and its mutants ?sod1, ?sod2, and ?sod1 ?sod2 were used. Yeast were subjected to the action of 1-14% DMSO for 1 h at 28 °C...
  43. Rona G, Herdeiro R, Mathias C, Torres F, Pereira M, Eleutherio E. CTT1 overexpression increases life span of calorie-restricted Saccharomyces cerevisiae deficient in Sod1. Biogerontology. 2015;16:343-51 pubmed publisher
    ..Old cells of sod1 strain also presented a premature induction of apoptosis...
  44. Böttinger L, Gornicka A, Czerwik T, Bragoszewski P, Loniewska Lwowska A, Schulze Specking A, et al. In vivo evidence for cooperation of Mia40 and Erv1 in the oxidation of mitochondrial proteins. Mol Biol Cell. 2012;23:3957-69 pubmed publisher
    ..Thus Mia40 in cooperation with Erv1 promotes the formation of two disulfide bonds in the substrate protein, ensuring the efficiency of oxidative folding in the intermembrane space of mitochondria. ..
  45. Muid K, Karakaya H, Koc A. Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process. Biochem Biophys Res Commun. 2014;444:260-3 pubmed publisher
    ..In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet ..
  46. Lin S, Culotta V. Suppression of oxidative damage by Saccharomyces cerevisiae ATX2, which encodes a manganese-trafficking protein that localizes to Golgi-like vesicles. Mol Cell Biol. 1996;16:6303-12 pubmed
    Oxygen toxicity in Saccharomyces cerevisiae lacking the copper/zinc superoxide dismutase (SOD1) can be suppressed by overexpression of the S. cerevisiae ATX2 gene...
  47. Reddehase S, Grumbt B, Neupert W, Hell K. The disulfide relay system of mitochondria is required for the biogenesis of mitochondrial Ccs1 and Sod1. J Mol Biol. 2009;385:331-8 pubmed publisher
    ..An important enzyme in this process is superoxide dismutase, Sod1, which converts superoxide radicals into water and hydrogen peroxide...
  48. Outten C, Falk R, Culotta V. Cellular factors required for protection from hyperoxia toxicity in Saccharomyces cerevisiae. Biochem J. 2005;388:93-101 pubmed
    ..Our findings are consistent with a model in which hyperoxia toxicity is manifested by superoxide-related damage and changes in the mitochondrial redox state. ..
  49. Budzinska M, Galganska H, Wojtkowska M, Stobienia O, Kmita H. Effects of VDAC isoforms on CuZn-superoxide dismutase activity in the intermembrane space of Saccharomyces cerevisiae mitochondria. Biochem Biophys Res Commun. 2007;357:1065-70 pubmed
    ..Thus, we conclude that in the case of S. cerevisiae both VDAC isoforms influence the IMS CuZnSOD activity and subsequently the expression levels of some mitochondrial proteins. ..
  50. Farooq M, Pracheil T, Dong Z, Xiao F, Liu Z. Mitochondrial DNA instability in cells lacking aconitase correlates with iron citrate toxicity. Oxid Med Cell Longev. 2013;2013:493536 pubmed publisher
    ..are the main source of superoxide radicals, which are converted to H2O2 through two superoxide dismutases, Sod1 and Sod2. H2O2 in turn reacts with Fe(2+) to generate very active hydroxyl radicals...
  51. Huang M, Xu Q, Mitsui K, Xu Z. PSK1 regulates expression of SOD1 involved in oxidative stress tolerance in yeast. FEMS Microbiol Lett. 2014;350:154-60 pubmed publisher
    ..Superoxide dismutase-1 (SOD1) mRNA and protein levels were lower in PSK1-deletion mutant than the wild type...
  52. Manfredini V, Roehrs R, Peralba M, Henriques J, Saffi J, Ramos A, et al. Glutathione peroxidase induction protects Saccharomyces cerevisiae sod1deltasod2delta double mutants against oxidative damage. Braz J Med Biol Res. 2004;37:159-65 pubmed
  53. Sea K, Sohn S, Durazo A, Sheng Y, Shaw B, Cao X, et al. Insights into the role of the unusual disulfide bond in copper-zinc superoxide dismutase. J Biol Chem. 2015;290:2405-18 pubmed publisher
    The functional and structural significance of the intrasubunit disulfide bond in copper-zinc superoxide dismutase (SOD1) was studied by characterizing mutant forms of human SOD1 (hSOD) and yeast SOD1 lacking the disulfide bond...
  54. Lamb A, Torres A, O Halloran T, Rosenzweig A. Heterodimer formation between superoxide dismutase and its copper chaperone. Biochemistry. 2000;39:14720-7 pubmed
    Copper, zinc superoxide dismutase (SOD1) is activated in vivo by the copper chaperone for superoxide dismutase (CCS). The molecular mechanisms by which CCS recognizes and docks with SOD1 for metal ion insertion are not well understood...
  55. Varabyova A, Topf U, Kwiatkowska P, Wrobel L, Kaus Drobek M, Chacinska A. Mia40 and MINOS act in parallel with Ccs1 in the biogenesis of mitochondrial Sod1. FEBS J. 2013;280:4943-59 pubmed publisher
    Superoxide dismutase 1 (Sod1) is a major superoxide-scavenging enzyme in the eukaryotic cell, and is localized in the cytosol and intermembrane space of mitochondria...
  56. Wei W, Smith N, Wu X, Kim H, Seravalli J, Khalimonchuk O, et al. YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes. Antioxid Redox Signal. 2014;21:1475-89 pubmed publisher
  57. Paumi C, Pickin K, Jarrar R, Herren C, Cowley S. Ycf1p attenuates basal level oxidative stress response in Saccharomyces cerevisiae. FEBS Lett. 2012;586:847-53 pubmed publisher
    ..Our results suggest that during acute salt stress increased Sod1p, Sod2p and Ctt1p activity is the main compensatory for the loss in Ycf1p function that results from reduced Ycf1p-dependent recycling of cellular GSH levels...
  58. McNaughton R, Reddi A, Clement M, Sharma A, Barnese K, Rosenfeld L, et al. Probing in vivo Mn2+ speciation and oxidative stress resistance in yeast cells with electron-nuclear double resonance spectroscopy. Proc Natl Acad Sci U S A. 2010;107:15335-9 pubmed publisher
  59. Slekar K, Kosman D, Culotta V. The yeast copper/zinc superoxide dismutase and the pentose phosphate pathway play overlapping roles in oxidative stress protection. J Biol Chem. 1996;271:28831-6 pubmed
    In Saccharomyces cerevisiae, loss of cytosolic superoxide dismutase (Sod1) results in several air-dependent mutant phenotypes, including methionine auxotrophy and oxygen sensitivity...
  60. Wood L, Thiele D. Transcriptional activation in yeast in response to copper deficiency involves copper-zinc superoxide dismutase. J Biol Chem. 2009;284:404-13 pubmed publisher
    ..Here we show that the copper-dependent enzyme Sod1 (Cu,Zn-superoxide dismutase) and its intracellular copper chaperone Ccs1 function in the activation of Mac1 in ..