Gene Symbol: SNU66
Description: U4/U6-U5 snRNP complex subunit SNU66
Alias: U4/U6-U5 snRNP complex subunit SNU66
Species: Saccharomyces cerevisiae S288c
Bouveret E, Rigaut G, Shevchenko A, Wilm M, Seraphin B. A Sm-like protein complex that participates in mRNA degradation. EMBO J. 2000;19:1661-71 pubmed
..These results indicate the involvement of a new conserved Sm-like protein complex and a new factor, Pat1p, in mRNA degradation and suggest a physical connection between decapping and exonuclease trimming. ..
Häcker I, Sander B, Golas M, Wolf E, Karagöz E, Kastner B, et al
. Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol. 2008;15:1206-12 pubmed publisher
..The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation. ..
Wilkinson C, Dittmar G, Ohi M, Uetz P, Jones N, Finley D. Ubiquitin-like protein Hub1 is required for pre-mRNA splicing and localization of an essential splicing factor in fission yeast. Curr Biol. 2004;14:2283-8 pubmed
..A screen for Hub1 interactors identified Snu66, a component of the U4/U6.U5 tri-snRNP splicing complex...
Brenner T, Guthrie C. Genetic analysis reveals a role for the C terminus of the Saccharomyces cerevisiae GTPase Snu114 during spliceosome activation. Genetics. 2005;170:1063-80 pubmed
..We propose that GTP hydrolysis results in a rearrangement between Prp8 and the C terminus of Snu114 that leads to release of U1 and U4, thus activating the spliceosome for catalysis. ..
Yeh C, Chang S, Chen J, Wang H, Chou Y, Wang C, et al
. The conserved AU dinucleotide at the 5' end of nascent U1 snRNA is optimized for the interaction with nuclear cap-binding-complex. Nucleic Acids Res. 2017;45:9679-9693 pubmed publisher
..Our data also provide a structural interpretation as to why the AU dinucleotide is conserved during evolution. ..
Taoka M, Yamauchi Y, Nobe Y, Masaki S, Nakayama H, Ishikawa H, et al
. An analytical platform for mass spectrometry-based identification and chemical analysis of RNA in ribonucleoprotein complexes. Nucleic Acids Res. 2009;37:e140 pubmed publisher
Huang Y, Chung C, Kao D, Kao T, Cheng S. Sad1 counteracts Brr2-mediated dissociation of U4/U6.U5 in tri-snRNP homeostasis. Mol Cell Biol. 2014;34:210-20 pubmed publisher
..Our results demonstrate a role of Sad1 in maintaining the integrity of the tri-snRNP by counteracting Brr2-mediated dissociation of tri-snRNP and provide insights into homeostasis of the tri-snRNP. ..
SORENSON M, Jha D, Ucles S, Flood D, Strahl B, Stevens S, et al
. Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae. RNA Biol. 2016;13:412-26 pubmed publisher
..Additionally, we found that deletion of SET2 reduces the association of the U2 and U5 snRNPs with chromatin. Thus, our study provides the first evidence that H3K36 methylation plays a role in co-transcriptional RNA splicing in yeast. ..
Luhtala N, Parker R. LSM1 over-expression in Saccharomyces cerevisiae depletes U6 snRNA levels. Nucleic Acids Res. 2009;37:5529-36 pubmed publisher
..These results suggest that inhibition of splicing and/or deadenylation may be effective therapies for Lsm1-over-expressing tumors. ..