SNF5

Summary

Gene Symbol: SNF5
Description: Snf5p
Alias: HAF4, SWI10, TYE4, Snf5p
Species: Saccharomyces cerevisiae S288c
Products:     SNF5

Top Publications

  1. Laurent B, Treitel M, Carlson M. Functional interdependence of the yeast SNF2, SNF5, and SNF6 proteins in transcriptional activation. Proc Natl Acad Sci U S A. 1991;88:2687-91 pubmed
    The SNF2, SNF5, and SNF6 genes of Saccharomyces cerevisiae are required for expression of a variety of differently regulated genes...
  2. Gong F, Fahy D, Smerdon M. Rad4-Rad23 interaction with SWI/SNF links ATP-dependent chromatin remodeling with nucleotide excision repair. Nat Struct Mol Biol. 2006;13:902-7 pubmed
    Chromatin rearrangement occurs during nucleotide excision repair (NER). Here we show that Snf6 and Snf5, two subunits of the SWI/SNF chromatin-remodeling complex in Saccharomyces cerevisiae, copurify with the NER damage-recognition ..
  3. Chai B, Huang J, Cairns B, Laurent B. Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair. Genes Dev. 2005;19:1656-61 pubmed
    ..We show that Swi/Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required following synapsis for completion of the recombinational repair event. ..
  4. Cairns B, Kim Y, Sayre M, Laurent B, Kornberg R. A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast. Proc Natl Acad Sci U S A. 1994;91:1950-4 pubmed
    A complex containing the products of the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 genes and four additional polypeptides has been purified from extracts of the yeast Saccharomyces cerevisiae...
  5. Prochasson P, Neely K, Hassan A, Li B, Workman J. Targeting activity is required for SWI/SNF function in vivo and is accomplished through two partially redundant activator-interaction domains. Mol Cell. 2003;12:983-90 pubmed
    ..Here we show that the N-terminal domain of Snf5 and the second quarter of Swi1 are sites of activation domain contact...
  6. Cairns B, Henry N, Kornberg R. TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9. Mol Cell Biol. 1996;16:3308-16 pubmed
    The SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products are all required for proper transcriptional control of many genes in the yeast Saccharomyces cerevisiae...
  7. Neely K, Hassan A, Brown C, Howe L, Workman J. Transcription activator interactions with multiple SWI/SNF subunits. Mol Cell Biol. 2002;22:1615-25 pubmed
    ..Using a photo-cross-linking label transfer strategy, we show that the Snf5, Swi1, and Swi2/Snf2 subunits are contacted by the yeast acidic activators, Gcn4 and Hap4, in the context of the ..
  8. Dimova D, Nackerdien Z, Furgeson S, Eguchi S, Osley M. A role for transcriptional repressors in targeting the yeast Swi/Snf complex. Mol Cell. 1999;4:75-83 pubmed
    ..b>Snf5p, Snf2p/Swi2p, and Swi3p, three components of the yeast Swi/Snf complex, coimmunoprecipitate with each Hir protein, ..
  9. Dechassa M, Zhang B, Horowitz Scherer R, Persinger J, Woodcock C, Peterson C, et al. Architecture of the SWI/SNF-nucleosome complex. Mol Cell Biol. 2008;28:6010-21 pubmed publisher
    ..The highly conserved Snf5 subunit associates with the histone octamer and not with nucleosomal DNA...

More Information

Publications49

  1. Yang X, Zaurin R, Beato M, Peterson C. Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacement. Nat Struct Mol Biol. 2007;14:540-7 pubmed
    ..Our data indicate that H2A-H2B dimer loss is not an obligatory consequence of ATP-dependent DNA translocation, and furthermore they suggest that SWI/SNF is composed of at least four interdependent modules. ..
  2. Smith C, Peterson C. A conserved Swi2/Snf2 ATPase motif couples ATP hydrolysis to chromatin remodeling. Mol Cell Biol. 2005;25:5880-92 pubmed
    ..Interestingly, motif V of the human Swi2p/Snf2p homolog, Brg1p, has been shown to be a possible hot spot for mutational alterations associated with cancers. ..
  3. Xu F, Zhang K, Grunstein M. Acetylation in histone H3 globular domain regulates gene expression in yeast. Cell. 2005;121:375-85 pubmed
    ..This allows recruitment of the nucleosome remodeling factor Snf5 and subsequent transcription...
  4. Ferreira M, Prochasson P, Berndt K, Workman J, Wright A. Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo. FEBS J. 2009;276:2557-65 pubmed publisher
    Interaction between acidic activation domains and the activator-binding domains of Swi1 and Snf5 of the yeast SWI/SNF chromatin remodeling complex has previously been characterized in vitro...
  5. Yoon S, Qiu H, Swanson M, Hinnebusch A. Recruitment of SWI/SNF by Gcn4p does not require Snf2p or Gcn5p but depends strongly on SWI/SNF integrity, SRB mediator, and SAGA. Mol Cell Biol. 2003;23:8829-45 pubmed
    ..It was shown previously that Snf2p/Swi2p, Snf5p, and Swi1p interact directly with Gcn4p in vitro...
  6. Smith C, Horowitz Scherer R, Flanagan J, Woodcock C, Peterson C. Structural analysis of the yeast SWI/SNF chromatin remodeling complex. Nat Struct Biol. 2003;10:141-5 pubmed
    ..We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs. ..
  7. Treich I, Cairns B, de los Santos T, Brewster E, Carlson M. SNF11, a new component of the yeast SNF-SWI complex that interacts with a conserved region of SNF2. Mol Cell Biol. 1995;15:4240-8 pubmed
    ..The complex has at least 10 components, including SNF2/SWI2, SNF5, SNF6, SWI1/ADR6, and SWI3, and has been widely conserved in eukaryotes...
  8. Cote J, Quinn J, Workman J, Peterson C. Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. Science. 1994;265:53-60 pubmed
    ..it is shown that the purified SWI/SNF complex is composed of 10 subunits and includes the SWI1, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products...
  9. Sen P, Vivas P, Dechassa M, Mooney A, Poirier M, Bartholomew B. The SnAC domain of SWI/SNF is a histone anchor required for remodeling. Mol Cell Biol. 2013;33:360-70 pubmed publisher
    ..While the SnAC domain targets both the ATPase domain and histones, the SnAC domain as a histone anchor plays a more critical role in remodeling because it is required to convert DNA translocation into nucleosome movement. ..
  10. Natarajan K, Jackson B, Zhou H, Winston F, Hinnebusch A. Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator. Mol Cell. 1999;4:657-64 pubmed
  11. Yoon S, Hinnebusch A. Mcm1p binding sites in ARG1 positively regulate Gcn4p binding and SWI/SNF recruitment. Biochem Biophys Res Commun. 2009;381:123-8 pubmed publisher
    ..We propose that Mcm1p contributes to active transcription at the ARG1 promoter by increasing the binding of the activator Gcn4p and by recruiting the co-activator complex SWI/SNF at ARG1 under Gcn4p-induced conditions. ..
  12. Breeden L, Nasmyth K. Cell cycle control of the yeast HO gene: cis- and trans-acting regulators. Cell. 1987;48:389-97 pubmed
    ..We also show that, in addition to SWI1 through SWI5, at least five other genes (SWI6 through SWI10) are required for HO transcription...
  13. Ferreira M, Flaherty K, Prochasson P. The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes. PLoS ONE. 2011;6:e21113 pubmed publisher
  14. Peterson C, Dingwall A, Scott M. Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. Proc Natl Acad Sci U S A. 1994;91:2905-8 pubmed
    The Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription...
  15. Zhang Y, Smith C, Saha A, Grill S, Mihardja S, Smith S, et al. DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC. Mol Cell. 2006;24:559-68 pubmed
    ..Such loop formation may provide a molecular basis for the biological functions of remodelers. ..
  16. Krajewski W, Reese J. SET domains of histone methyltransferases recognize ISWI-remodeled nucleosomal species. Mol Cell Biol. 2010;30:552-64 pubmed publisher
    ..Our study reveals novel insights into the mechanism of how SET domains recognize different chromatin states and specify histone methylation at active loci. ..
  17. Düring L, Thorsen M, Petersen D, Køster B, Jensen T, Holmberg S. MRN1 implicates chromatin remodeling complexes and architectural factors in mRNA maturation. PLoS ONE. 2012;7:e44373 pubmed publisher
  18. Minard L, Lin L, Schultz M. SWI/SNF and Asf1 independently promote derepression of the DNA damage response genes under conditions of replication stress. PLoS ONE. 2011;6:e21633 pubmed publisher
    ..Collectively, our results show that the DDR genes fall into a class in which Asf1 and SWI/SNF independently control transcriptional induction. ..
  19. Bonazzi V, Medjkane S, Quignon F, Delattre O. Complementation analyses suggest species-specific functions of the SNF5 homology domain. Biochem Biophys Res Commun. 2005;336:634-8 pubmed
    ..In contrast, it has two homologs in the yeast Saccharomyces cerevisiae, SNF5 and SFH1 which encode core components of the ySWI/SNF and RSC complexes, respectively...
  20. Zhang L, Jones K, Smerdon M, Gong F. Assays for chromatin remodeling during nucleotide excision repair in Saccharomyces cerevisiae. Methods. 2009;48:19-22 pubmed publisher
    ..We have used the methods described here to demonstrate that the SWI/SNF chromatin remodeling complex is involved in chromatin rearrangement during NER. ..
  21. Yoon S, Govind C, Qiu H, Kim S, Dong J, Hinnebusch A. Recruitment of the ArgR/Mcm1p repressor is stimulated by the activator Gcn4p: a self-checking activation mechanism. Proc Natl Acad Sci U S A. 2004;101:11713-8 pubmed
    ..By recruiting an arginine-regulated repressor, Gcn4p can precisely modulate its activation function at ARG1 according to the availability of arginine. ..
  22. Laurent B, Treitel M, Carlson M. The SNF5 protein of Saccharomyces cerevisiae is a glutamine- and proline-rich transcriptional activator that affects expression of a broad spectrum of genes. Mol Cell Biol. 1990;10:5616-25 pubmed
    The Saccharomyces cerevisiae SNF5 gene affects expression of both glucose- and phosphate-regulated genes and appears to function in transcription...
  23. Peterson C, Herskowitz I. Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription. Cell. 1992;68:573-83 pubmed
    ..SWI1 is identical to ADR6, which encodes a positive regulator of ADH1 and ADH2. Transcription of HO also requires SNF5 and SNF6...
  24. Schulze J, Kane C, Ruiz Manzano A. The YEATS domain of Taf14 in Saccharomyces cerevisiae has a negative impact on cell growth. Mol Genet Genomics. 2010;283:365-80 pubmed publisher
    ..Thus, this highly conserved domain should be considered part of a negative regulatory loop in cell growth. ..
  25. Dechassa M, Hota S, Sen P, Chatterjee N, Prasad P, Bartholomew B. Disparity in the DNA translocase domains of SWI/SNF and ISW2. Nucleic Acids Res. 2012;40:4412-21 pubmed publisher
    ..These differences are likely mediated through interactions with the histone surface. The placement of SWI/SNF between the octamer and DNA could make it easier to disrupt histone-DNA interactions. ..
  26. Smith C, Peterson C. Coupling tandem affinity purification and quantitative tyrosine iodination to determine subunit stoichiometry of protein complexes. Methods. 2003;31:104-9 pubmed
  27. Sterner D, Grant P, Roberts S, Duggan L, Belotserkovskaya R, Pacella L, et al. Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol. 1999;19:86-98 pubmed
    ..Loss of either of these causes slight impairment in vivo, but loss of both is highly detrimental to growth and transcription. ..
  28. Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher
    ..Thus, cells must have a way of ensuring a proper balance between actin assembly pathways. ..
  29. Barrales R, Jimenez J, Ibeas J. Identification of novel activation mechanisms for FLO11 regulation in Saccharomyces cerevisiae. Genetics. 2008;178:145-56 pubmed publisher
    ..In addition to previously characterized activators, we identified a number of novel FLO11 activators, which reveal the pH response pathway and chromatin-remodeling complexes as central elements involved in FLO11 activation. ..
  30. Zhang L, Fletcher A, Cheung V, Winston F, Stargell L. Spn1 regulates the recruitment of Spt6 and the Swi/Snf complex during transcriptional activation by RNA polymerase II. Mol Cell Biol. 2008;28:1393-403 pubmed
    ..These findings link Spn1 functions to the transition from an inactive to an actively transcribing RNAPII complex at a postrecruitment-regulated promoter. ..
  31. Recht J, Osley M. Mutations in both the structured domain and N-terminus of histone H2B bypass the requirement for Swi-Snf in yeast. EMBO J. 1999;18:229-40 pubmed
    ..The phenotypes of double gcn5Deltasnf5Delta mutants suggest that histone acetylation may play both positive and negative roles in the activity of the Swi-Snf-remodeling factor. ..
  32. Sen P, Ghosh S, Pugh B, Bartholomew B. A new, highly conserved domain in Swi2/Snf2 is required for SWI/SNF remodeling. Nucleic Acids Res. 2011;39:9155-66 pubmed publisher
    ..The SnAC domain positively regulates the catalytic activity of the ATPase domain of SWI/SNF to hydrolyze ATP without significantly affecting its affinity for ATP. ..
  33. Barrales R, Korber P, Jimenez J, Ibeas J. Chromatin modulation at the FLO11 promoter of Saccharomyces cerevisiae by HDAC and Swi/Snf complexes. Genetics. 2012;191:791-803 pubmed publisher
    ..We show that the regulation of chromatin structure represents an important layer of control in the highly complex regulation of the FLO11 promoter. ..
  34. Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, et al. Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex. Genes Dev. 2015;29:591-602 pubmed publisher
    ..Together, these findings identify a novel regulator of Mec1 kinase activity and suggest that ATP-dependent chromatin remodeling complexes can regulate nonchromatin substrates such as a checkpoint kinase. ..
  35. Du Z, Zhang Y, Li L. The Yeast Prion [SWI(+)] Abolishes Multicellular Growth by Triggering Conformational Changes of Multiple Regulators Required for Flocculin Gene Expression. Cell Rep. 2015;13:2865-78 pubmed publisher
  36. Duina A, Winston F. Analysis of a mutant histone H3 that perturbs the association of Swi/Snf with chromatin. Mol Cell Biol. 2004;24:561-72 pubmed
    ..Taken together, our results strongly suggest that the integrity of the globular domain of histone H3 is an important determinant in the ability of Swi/Snf to associate with chromatin. ..
  37. Roberts S, Winston F. Essential functional interactions of SAGA, a Saccharomyces cerevisiae complex of Spt, Ada, and Gcn5 proteins, with the Snf/Swi and Srb/mediator complexes. Genetics. 1997;147:451-65 pubmed
    ..These findings suggest that SAGA has multiple activities and plays critical roles in transcription by RNA polymerase II. ..
  38. Neigeborn L, Carlson M. Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae. Genetics. 1984;108:845-58 pubmed
    ..snf1 (sucrose nonfermenting) alleles and also defined five new complementation groups, designated snf2, snf3, snf4, snf5, and snf6...
  39. Neigeborn L, Rubin K, Carlson M. Suppressors of SNF2 mutations restore invertase derepression and cause temperature-sensitive lethality in yeast. Genetics. 1986;112:741-53 pubmed
    ..The ssn20 mutations also suppressed the defects in invertase derepression caused by snf5 and snf6 mutations, and selection for invertase-producing revertants of snf5 mutants yielded only additional ssn20 ..
  40. Hirschhorn J, Brown S, Clark C, Winston F. Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure. Genes Dev. 1992;6:2288-98 pubmed
    ..Recent evidence suggests that a functionally related set of yeast transcriptional activators (SNF2/SWI2, SNF5, SNF6, SWI1, and SWI3), required for transcription of a diverse set of genes, may affect chromatin structure...