Genomes and Genes
Gene Symbol: SNF2
Description: SWI/SNF catalytic subunit SNF2
Alias: GAM1, HAF1, SWI2, TYE3, SWI/SNF catalytic subunit SNF2
Species: Saccharomyces cerevisiae S288c
- Yang X, Zaurin R, Beato M, Peterson C. Swi3p controls SWI/SNF assembly and ATP-dependent H2A-H2B displacement. Nat Struct Mol Biol. 2007;14:540-7 pubmed..Our data indicate that H2A-H2B dimer loss is not an obligatory consequence of ATP-dependent DNA translocation, and furthermore they suggest that SWI/SNF is composed of at least four interdependent modules. ..
- Szerlong H, Saha A, Cairns B. The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling. EMBO J. 2003;22:3175-87 pubmed..We propose that Arp7/9 dimers function with DNA bending proteins to facilitate proper chromatin architecture and complex- complex interactions. ..
- Cairns B, Kim Y, Sayre M, Laurent B, Kornberg R. A multisubunit complex containing the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products isolated from yeast. Proc Natl Acad Sci U S A. 1994;91:1950-4 pubmedA complex containing the products of the SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 genes and four additional polypeptides has been purified from extracts of the yeast Saccharomyces cerevisiae...
- Smith C, Horowitz Scherer R, Flanagan J, Woodcock C, Peterson C. Structural analysis of the yeast SWI/SNF chromatin remodeling complex. Nat Struct Biol. 2003;10:141-5 pubmed..We also report a three-dimensional reconstruction of yeast SWI/SNF derived from electron micrographs. ..
- Sudarsanam P, Cao Y, Wu L, Laurent B, Winston F. The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5. EMBO J. 1999;18:3101-6 pubmed
- Cote J, Quinn J, Workman J, Peterson C. Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex. Science. 1994;265:53-60 pubmed..Here it is shown that the purified SWI/SNF complex is composed of 10 subunits and includes the SWI1, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products...
- Shivaswamy S, Iyer V. Stress-dependent dynamics of global chromatin remodeling in yeast: dual role for SWI/SNF in the heat shock stress response. Mol Cell Biol. 2008;28:2221-34 pubmed publisher..Third, Snf2 was recruited to ribosomal protein genes and Hsf1 target genes, and its occupancy of this large set of genes was ..
- Dechassa M, Zhang B, Horowitz Scherer R, Persinger J, Woodcock C, Peterson C, et al. Architecture of the SWI/SNF-nucleosome complex. Mol Cell Biol. 2008;28:6010-21 pubmed publisher..The DNA site-directed cross-linking revealed that the catalytic subunit Swi2/Snf2 is associated with nucleosomes two helical turns from the dyad axis and that the Snf6 subunit is proximal to the ..
- Erkina T, Tschetter P, Erkine A. Different requirements of the SWI/SNF complex for robust nucleosome displacement at promoters of heat shock factor and Msn2- and Msn4-regulated heat shock genes. Mol Cell Biol. 2008;28:1207-17 pubmed..We demonstrate that the deletion of SNF2, an ATPase activity-containing subunit of the chromatin remodeling SWI/SNF complex, eliminates histone displacement,..
- Barbaric S, Luckenbach T, Schmid A, Blaschke D, Hörz W, Korber P. Redundancy of chromatin remodeling pathways for the induction of the yeast PHO5 promoter in vivo. J Biol Chem. 2007;282:27610-21 pubmed..Although induction of PHO8 is strictly dependent on Snf2 and Gcn5, there is no chromatin remodeler identified so far that would be essential for the opening of PHO5 ..
- Szerlong H, Hinata K, Viswanathan R, Erdjument Bromage H, Tempst P, Cairns B. The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases. Nat Struct Mol Biol. 2008;15:469-76 pubmed publisher..Taken together, we define the role of the HSA domain and provide evidence for a regulatory relationship involving the ARP-HSA module and two new functional domains conserved in remodeler ATPases that contain ARPs. ..
- Flanagan J, Peterson C. A role for the yeast SWI/SNF complex in DNA replication. Nucleic Acids Res. 1999;27:2022-8 pubmed
- Gkikopoulos T, Havas K, Dewar H, Owen Hughes T. SWI/SNF and Asf1p cooperate to displace histones during induction of the saccharomyces cerevisiae HO promoter. Mol Cell Biol. 2009;29:4057-66 pubmed publisher..The ATPase Snf2 is required for these alterations, but the histone acetyltransferase Gcn5 is not...
- Sterner D, Grant P, Roberts S, Duggan L, Belotserkovskaya R, Pacella L, et al. Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Mol Cell Biol. 1999;19:86-98 pubmed..Loss of either of these causes slight impairment in vivo, but loss of both is highly detrimental to growth and transcription. ..
- Smith C, Peterson C. A conserved Swi2/Snf2 ATPase motif couples ATP hydrolysis to chromatin remodeling. Mol Cell Biol. 2005;25:5880-92 pubmed..Swi2/Snf2 is the catalytic subunit of SWI/SNF, and it is the founding member of a novel subfamily of the SF2 superfamily of ..
- Chai B, Huang J, Cairns B, Laurent B. Distinct roles for the RSC and Swi/Snf ATP-dependent chromatin remodelers in DNA double-strand break repair. Genes Dev. 2005;19:1656-61 pubmed..We show that Swi/Snf is required earlier, at or preceding the strand invasion step of HR, while RSC is required following synapsis for completion of the recombinational repair event. ..
- Peterson C, Dingwall A, Scott M. Five SWI/SNF gene products are components of a large multisubunit complex required for transcriptional enhancement. Proc Natl Acad Sci U S A. 1994;91:2905-8 pubmedThe Saccharomyces cerevisiae SWI1, SWI2 (SNF2), SWI3, SNF5, and SNF6 gene products play a crucial role in the regulation of transcription...
- Peterson C, Herskowitz I. Characterization of the yeast SWI1, SWI2, and SWI3 genes, which encode a global activator of transcription. Cell. 1992;68:573-83 pubmedThe yeast SWI1, SWI2 (SNF2), and SWI3 genes are required for transcription of HO and INO1 genes...
- Stern M, Jensen R, Herskowitz I. Five SWI genes are required for expression of the HO gene in yeast. J Mol Biol. 1984;178:853-68 pubmed..These results indicate that the SWI genes function in some way as positive regulators of HO expression and have additional cellular roles. ..
- Ryan D, Owen Hughes T. Snf2-family proteins: chromatin remodellers for any occasion. Curr Opin Chem Biol. 2011;15:649-56 pubmed publisher..b>Snf2 proteins are a family of helicase-like proteins that direct energy derived from ATP hydrolysis into the mechanical ..
- Boukaba A, Georgieva E, Myers F, Thorne A, López Rodas G, Crane Robinson C, et al. A short-range gradient of histone H3 acetylation and Tup1p redistribution at the promoter of the Saccharomyces cerevisiae SUC2 gene. J Biol Chem. 2004;279:7678-84 pubmed..Overall levels of both H3 and H4 acetylation rise on derepression. Mutation of GCN5 or SNF2 lead to substantially reduced SUC2 expression; in gnc5 there is no reduction in basal H3 acetylation, but large ..
- Cairns B, Schlichter A, Erdjument Bromage H, Tempst P, Kornberg R, Winston F. Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains. Mol Cell. 1999;4:715-23 pubmed..Therefore, these domains are required for RSC function. Additional genetic analysis provides further evidence that RSC function is related to transcriptional control. ..
- Hassan A, Awad S, Al Natour Z, Othman S, Mustafa F, Rizvi T. Selective recognition of acetylated histones by bromodomains in transcriptional co-activators. Biochem J. 2007;402:125-33 pubmed..Our results reveal that the Swi2/Snf2 bromodomain interacts with various acetylated H3 and H4 peptides, whereas the Gcn5 bromodomain interacts only with ..
- Yoon S, Qiu H, Swanson M, Hinnebusch A. Recruitment of SWI/SNF by Gcn4p does not require Snf2p or Gcn5p but depends strongly on SWI/SNF integrity, SRB mediator, and SAGA. Mol Cell Biol. 2003;23:8829-45 pubmed..We suggest that SWI/SNF recruitment is enhanced by cooperative interactions with subunits of SRB mediator and SAGA recruited by Gcn4p to the same promoter but is insensitive to histone H3 acetylation by Gcn5p. ..
- Neely K, Hassan A, Brown C, Howe L, Workman J. Transcription activator interactions with multiple SWI/SNF subunits. Mol Cell Biol. 2002;22:1615-25 pubmed..Using a photo-cross-linking label transfer strategy, we show that the Snf5, Swi1, and Swi2/Snf2 subunits are contacted by the yeast acidic activators, Gcn4 and Hap4, in the context of the intact native SWI/..
- Davie J, Kane C. Genetic interactions between TFIIS and the Swi-Snf chromatin-remodeling complex. Mol Cell Biol. 2000;20:5960-73 pubmed..These include UBP3, KEX2, STT4, and SWI2/SNF2...
- Natarajan K, Jackson B, Zhou H, Winston F, Hinnebusch A. Transcriptional activation by Gcn4p involves independent interactions with the SWI/SNF complex and the SRB/mediator. Mol Cell. 1999;4:657-64 pubmed
- Hassan A, Prochasson P, Neely K, Galasinski S, Chandy M, Carrozza M, et al. Function and selectivity of bromodomains in anchoring chromatin-modifying complexes to promoter nucleosomes. Cell. 2002;111:369-79 pubmed..promoter occupancy by SWI/SNF and SAGA in the absence of transcription activators requires the bromodomains of the Swi2/Snf2 and Gcn5 subunits, respectively, and nucleosome acetylation...
- Cosma M, Tanaka T, Nasmyth K. Ordered recruitment of transcription and chromatin remodeling factors to a cell cycle- and developmentally regulated promoter. Cell. 1999;97:299-311 pubmed..Swi5p remains at HO for only 5 min. Swi/Snf's and SAGA's subsequent persistence at HO is self sustaining and constitutes an "epigenetic memory" of HO's transient interaction with Swi5p. ..
- Laurent B, Treich I, Carlson M. The yeast SNF2/SWI2 protein has DNA-stimulated ATPase activity required for transcriptional activation. Genes Dev. 1993;7:583-91 pubmedThe yeast SNF2 (SWI2) protein functions with SNF5, SNF6, SWI1, and SWI3 in the transcriptional activation of many differently regulated genes. These proteins appear to facilitate activation by gene-specific regulatory proteins...
- Hirschhorn J, Brown S, Clark C, Winston F. Evidence that SNF2/SWI2 and SNF5 activate transcription in yeast by altering chromatin structure. Genes Dev. 1992;6:2288-98 pubmed..Recent evidence suggests that a functionally related set of yeast transcriptional activators (SNF2/SWI2, SNF5, SNF6, SWI1, and SWI3), required for transcription of a diverse set of genes, may affect chromatin structure...
- Kim Y, McLaughlin N, Lindstrom K, Tsukiyama T, Clark D. Activation of Saccharomyces cerevisiae HIS3 results in Gcn4p-dependent, SWI/SNF-dependent mobilization of nucleosomes over the entire gene. Mol Cell Biol. 2006;26:8607-22 pubmed..We propose that Gcn4p stimulates nucleosome mobilization over the entire HIS3 gene by the SWI/SNF complex. We suggest that the net effect of interplay among remodeling machines at HIS3 is to create a highly dynamic chromatin structure. ..
- Korber P, Barbaric S, Luckenbach T, Schmid A, Schermer U, Blaschke D, et al. The histone chaperone Asf1 increases the rate of histone eviction at the yeast PHO5 and PHO8 promoters. J Biol Chem. 2006;281:5539-45 pubmed..e. they both contribute toward the same outcome without being mutually strictly dependent. ..
- Wong K, Struhl K. The Cyc8-Tup1 complex inhibits transcription primarily by masking the activation domain of the recruiting protein. Genes Dev. 2011;25:2525-39 pubmed publisher..We suggest that the corepressor function of Cyc8-Tup1 makes only a modest contribution to expression of target genes, specifically to keep expression levels below the nonactivated state. ..
- Neigeborn L, Rubin K, Carlson M. Suppressors of SNF2 mutations restore invertase derepression and cause temperature-sensitive lethality in yeast. Genetics. 1986;112:741-53 pubmedMutations in the SNF2 gene of Saccharomyces cerevisiae prevent derepression of the SUC2 (invertase) gene, and other glucose-repressible genes, in response to glucose deprivation...
- Kamisaka Y, Tomita N, Kimura K, Kainou K, Uemura H. DGA1 (diacylglycerol acyltransferase gene) overexpression and leucine biosynthesis significantly increase lipid accumulation in the Deltasnf2 disruptant of Saccharomyces cerevisiae. Biochem J. 2007;408:61-8 pubmedWe previously found that SNF2, a gene encoding a transcription factor forming part of the SWI/SNF (switching/sucrose non-fermenting) chromatin-remodelling complex, is involved in lipid accumulation, because the Deltasnf2 disruptant of ..
- Biswas D, Imbalzano A, Eriksson P, Yu Y, Stillman D. Role for Nhp6, Gcn5, and the Swi/Snf complex in stimulating formation of the TATA-binding protein-TFIIA-DNA complex. Mol Cell Biol. 2004;24:8312-21 pubmed..lethal in strains with disruptions of either GCN5, encoding the histone acetyltransferase in the SAGA complex, or SWI2, encoding the catalytic subunit of the Swi/Snf chromatin remodeling complex...
- Tran H, Steger D, Iyer V, Johnson A. The chromo domain protein chd1p from budding yeast is an ATP-dependent chromatin-modifying factor. EMBO J. 2000;19:2323-31 pubmed..Taken together, these results suggest that Chd1p functions as a nucleosome remodeling factor, and that Chd1p may share overlapping roles with the SWI-SNF complex to regulate transcription. ..
- Sinha M, Watanabe S, Johnson A, Moazed D, Peterson C. Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling. Cell. 2009;138:1109-21 pubmed publisher..These results suggest that recombinational repair in the context of heterochromatin presents additional constraints that can be overcome by ATP-dependent chromatin-remodeling enzymes. ..
- Zhang Y, Anderson S, French S, Sikes M, Viktorovskaya O, Huband J, et al. The SWI/SNF chromatin remodeling complex influences transcription by RNA polymerase I in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e56793 pubmed publisher..Our findings demonstrate that SWI/SNF influences the most robust transcription machinery in proliferating cells. ..
- Neigeborn L, Carlson M. Mutations causing constitutive invertase synthesis in yeast: genetic interactions with snf mutations. Genetics. 1987;115:247-53 pubmed..The constitutive mutation tup1 was found to resemble ssn6 in its genetic interactions with snf mutations. These findings suggest that CID1, REG1 and HXK2 are functionally distinct from SSN6 and TUP1. ..
- Liu X, Li M, Xia X, Li X, Chen Z. Mechanism of chromatin remodelling revealed by the Snf2-nucleosome structure. Nature. 2017;544:440-445 pubmed publisher..Here we report the cryo-electron microscopy structure of chromatin remodeller Switch/sucrose non-fermentable (SWI2/SNF2) from Saccharomyces cerevisiae bound to the nucleosome...
- Wimalarathna R, Pan P, Shen C. Co-dependent recruitment of Ino80p and Snf2p is required for yeast CUP1 activation. Biochem Cell Biol. 2014;92:69-75 pubmed publisher..This inviability is due to the lack of CUP1 expression in ino80? and snf2? cells...
- Zhang Q, Chakravarty S, Ghersi D, Zeng L, Plotnikov A, Sanchez R, et al. Biochemical profiling of histone binding selectivity of the yeast bromodomain family. PLoS ONE. 2010;5:e8903 pubmed publisher..Finally, electrostatic interactions appear to be a primary determining factor that guides productive association between a bromodomain and a lysine-acetylated histone. ..
- Kapoor P, Bao Y, Xiao J, Luo J, Shen J, Persinger J, et al. Regulation of Mec1 kinase activity by the SWI/SNF chromatin remodeling complex. Genes Dev. 2015;29:591-602 pubmed publisher..In vivo, Mec1 activity is reduced by the deletion of Snf2, the core ATPase subunit of the SWI/SNF complex...
- Malik S, Chaurasia P, Lahudkar S, Uprety B, Bhaumik S. Rad26p regulates the occupancy of histone H2A-H2B dimer at the active genes in vivo. Nucleic Acids Res. 2012;40:3348-63 pubmed publisher..Similar results are also found at other inducible non-GAL genes. Collectively, our results define a new role of Rad26p in orchestrating chromatin structure and hence transcription in vivo. ..
- Ford J, Odeyale O, Eskandar A, Kouba N, Shen C. A SWI/SNF- and INO80-dependent nucleosome movement at the INO1 promoter. Biochem Biophys Res Commun. 2007;361:974-9 pubmed..In light of these findings, we suggest that nucleosomes subject to local mobilization are also targets for local histone modifications. ..
- Krishnamurthy M, Dugan A, Nwokoye A, Fung Y, Lancia J, Majmudar C, et al. Caught in the act: covalent cross-linking captures activator-coactivator interactions in vivo. ACS Chem Biol. 2011;6:1321-6 pubmed publisher..through in vivo photo-cross-linking that VP16 contacts the Swi/Snf chromatin-remodeling complex through the ATPase Snf2(BRG1/BRM) and the subunit Snf5 with two distinct regions of the activation domain...
- Awad A, Venkataramanan S, Nag A, Galivanche A, Bradley M, Neves L, et al. Chromatin-remodeling SWI/SNF complex regulates coenzyme Q6 synthesis and a metabolic shift to respiration in yeast. J Biol Chem. 2017;292:14851-14866 pubmed publisher..We find that a nutrient-dependent decrease in Snf2 leads to an increase in splicing of the PTC7 transcript...
- Musladin S, Krietenstein N, Korber P, Barbaric S. The RSC chromatin remodeling complex has a crucial role in the complete remodeler set for yeast PHO5 promoter opening. Nucleic Acids Res. 2014;42:4270-82 pubmed publisher..Strikingly, combined absence of RSC and Isw1/Chd1 or Snf2 abolished for the first time promoter opening on otherwise sufficient induction in vivo...
- Sanz A, Garcia R, Rodriguez Peña J, Diez Muñiz S, Nombela C, Peterson C, et al. Chromatin remodeling by the SWI/SNF complex is essential for transcription mediated by the yeast cell wall integrity MAPK pathway. Mol Biol Cell. 2012;23:2805-17 pubmed publisher..Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress. ..
- Zhang L, Fletcher A, Cheung V, Winston F, Stargell L. Spn1 regulates the recruitment of Spt6 and the Swi/Snf complex during transcriptional activation by RNA polymerase II. Mol Cell Biol. 2008;28:1393-403 pubmed..These findings link Spn1 functions to the transition from an inactive to an actively transcribing RNAPII complex at a postrecruitment-regulated promoter. ..
- Sen P, Ghosh S, Pugh B, Bartholomew B. A new, highly conserved domain in Swi2/Snf2 is required for SWI/SNF remodeling. Nucleic Acids Res. 2011;39:9155-66 pubmed publisher..One of these domains, termed SnAC (Snf2 ATP Coupling), is conserved in all eukaryotic SWI/SNF complexes and is located between the ATPase and A-T hook ..
- Gkikopoulos T, Singh V, Tsui K, Awad S, Renshaw M, Scholfield P, et al. The SWI/SNF complex acts to constrain distribution of the centromeric histone variant Cse4. EMBO J. 2011;30:1919-27 pubmed publisher..Deletion of the gene encoding the Snf2 subunit of the complex was found to cause partial redistribution of the centromeric histone variant Cse4 to sites ..
- Dechassa M, Hota S, Sen P, Chatterjee N, Prasad P, Bartholomew B. Disparity in the DNA translocase domains of SWI/SNF and ISW2. Nucleic Acids Res. 2012;40:4412-21 pubmed publisher..These differences are likely mediated through interactions with the histone surface. The placement of SWI/SNF between the octamer and DNA could make it easier to disrupt histone-DNA interactions. ..
- Breeden L, Nasmyth K. Cell cycle control of the yeast HO gene: cis- and trans-acting regulators. Cell. 1987;48:389-97 pubmed..SWI4 and SWI6 are specifically required for CACGA4-mediated activation of transcription. SWI1, SWI2, and SWI5 are required for transcription from sequences physically separate from and independent of the CACGA4 ..
- Weider M, Schroder A, Klebl F, Sauer N. A novel mechanism for target gene-specific SWI/SNF recruitment via the Snf2p N-terminus. Nucleic Acids Res. 2011;39:4088-98 pubmed publisher..We present a novel mechanism of target gene-specific SWI/SNF recruitment via Vhr1p and a conserved N-terminal Snf2p domain. ..
- Kamisaka Y, Kimura K, Uemura H, Yamaoka M. Overexpression of the active diacylglycerol acyltransferase variant transforms Saccharomyces cerevisiae into an oleaginous yeast. Appl Microbiol Biotechnol. 2013;97:7345-55 pubmed publisher..Dga1p lacking the N-terminal 29 amino acids (Dga1?Np), which was previously found to be an active form in the ?snf2 mutant...
- Hayashi N, Kobayashi M, Shimizu H, Yamamoto K, Murakami S, Nishimoto T. Mutations in Ran system affected telomere silencing in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2007;363:788-94 pubmed..Furthermore, hyperphosphorylated Sir3 protein accumulated in the rna1-1 mutant. These results suggest that RanGAP is required for the heterochromatin structure at the telomere in budding yeast. ..
- Cooper B. Collateral gene expression changes induced by distinct plant viruses during the hypersensitive resistance reaction in Chenopodium amaranticolor. Plant J. 2001;26:339-49 pubmed..The potential role of DESCA genes in a C. amaranticolor multivirus defense response with regard to their levels and time of gene expression is discussed. ..
- Wohlschlegel J, Johnson E, Reed S, Yates J. Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem. 2004;279:45662-8 pubmed..Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes. ..
- Biddick R, Law G, Chin K, Young E. The transcriptional coactivators SAGA, SWI/SNF, and mediator make distinct contributions to activation of glucose-repressed genes. J Biol Chem. 2008;283:33101-9 pubmed publisher..Finally, we found that activator overexpression can compensate for the loss of SWI/SNF but not for the loss of SAGA. ..
- Cairns B, Henry N, Kornberg R. TFG/TAF30/ANC1, a component of the yeast SWI/SNF complex that is similar to the leukemogenic proteins ENL and AF-9. Mol Cell Biol. 1996;16:3308-16 pubmedThe SWI1/ADR6, SWI2/SNF2, SWI3, SNF5, and SNF6 gene products are all required for proper transcriptional control of many genes in the yeast Saccharomyces cerevisiae...
- Santisteban M, Arents G, Moudrianakis E, Smith M. Histone octamer function in vivo: mutations in the dimer-tetramer interfaces disrupt both gene activation and repression. EMBO J. 1997;16:2493-506 pubmed..These results demonstrate the critical role of histone dimer-tetramer interactions in vivo, and define their essential role in the expression of genes regulating G1 cell cycle progression. ..
- Wiest N, Houghtaling S, Sanchez J, Tomkinson A, Osley M. The SWI/SNF ATP-dependent nucleosome remodeler promotes resection initiation at a DNA double-strand break in yeast. Nucleic Acids Res. 2017;45:5887-5900 pubmed publisher..These phenotypes are correlated with a delay in the eviction of nucleosomes surrounding the DSB. We propose that SWI/SNF orchestrates the recruitment of a pool of MRX that is specifically dedicated to HR. ..