SNF1

Summary

Gene Symbol: SNF1
Description: Snf1p
Alias: CAT1, CCR1, GLC2, HAF3, PAS14
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. ncbi Hxk2 regulates the phosphorylation state of Mig1 and therefore its nucleocytoplasmic distribution
    Deifilia Ahuatzi
    Departamento de Bioquimica y Biologia Molecular, Universidad de Oviedo, Campus del Cristo, Edificio Santiago Gascon, 33006 Oviedo, Spain
    J Biol Chem 282:4485-93. 2007
  2. doi The role of the Snf1 kinase in the adaptive response of Saccharomyces cerevisiae to alkaline pH stress
    Antonio Casamayor
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Spain
    Biochem J 444:39-49. 2012
  3. pmc Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase
    Amparo Ruiz
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:6349-54. 2011
  4. pmc A dual role for PP1 in shaping the Msn2-dependent transcriptional response to glucose starvation
    Veerle De Wever
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Biochemistry, University of Vienna and Ludwig Boltzmann Forschungsstelle für Biochemie, Vienna, Austria
    EMBO J 24:4115-23. 2005
  5. doi The pathway by which the yeast protein kinase Snf1p controls acquisition of sodium tolerance is different from that mediating glucose regulation
    Tian Ye
    Department of Cell and Molecular Biology Microbiology, University of Gothenburg, Box 462, S 40530 Göteborg, Sweden
    Microbiology 154:2814-26. 2008
  6. pmc Snf1p regulates Gcn5p transcriptional activity by antagonizing Spt3p
    Yang Liu
    Program in Genetics, Michigan State University, East Lansing, Michigan 48824, USA
    Genetics 184:91-105. 2010
  7. pmc Roles of the Snf1-activating kinases during nitrogen limitation and pseudohyphal differentiation in Saccharomyces cerevisiae
    Marianna Orlova
    Department of Biological Sciences, University of Wisconsin Milwaukee, Milwaukee, WI 53211, USA
    Eukaryot Cell 9:208-14. 2010
  8. pmc Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2
    Nitnipa Soontorngun
    Department of Medicine, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada H3A 1A1
    Mol Cell Biol 27:7895-905. 2007
  9. ncbi Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family
    Vinod Nayak
    The Wistar Institute, University of Pennsylvania, Philadelphia 19104, USA
    Structure 14:477-85. 2006
  10. doi Regulatory mechanism for expression of GPX1 in response to glucose starvation and Ca in Saccharomyces cerevisiae: involvement of Snf1 and Ras/cAMP pathway in Ca signaling
    Takumi Ohdate
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Genes Cells 15:59-75. 2010

Research Grants

Scientific Experts

  • ELTON YOUNG
  • Min Hao Kuo
  • P W Sherwood
  • S A Johnston
  • R R Naik
  • MARIAN B CARLSON
  • Z Wang
  • H Zhou
  • Francisco Portillo
  • Martin C Schmidt
  • Rhonda R McCartney
  • Yang Liu
  • Sergei Kuchin
  • Margaret K Shirra
  • Eric M Rubenstein
  • Renata Usaite
  • Marianna Orlova
  • Karin Elbing
  • Tian Ye
  • Amparo Ruiz
  • Nandita Nath
  • Michael J Rudolph
  • Xinjing Xu
  • S Kuchin
  • Gabriele A Amodeo
  • Shelley L Berger
  • Karen M Arndt
  • Seung Pyo Hong
  • Wan Sheng Lo
  • Michel Becuwe
  • Antonio Casamayor
  • Marenda A Wilson
  • Faith V Mayer
  • Jin Ying Lu
  • Takumi Ohdate
  • Simmanjeet Mangat
  • Sooraj Ratnakumar
  • P Sanz
  • Anna Leech
  • Ulrike von Plehwe
  • Jacob H Boysen
  • Melissa A MacKinnon
  • Sandra Holbein
  • Jens Nielsen
  • Lisbeth Olsson
  • Liang Tong
  • Kevan M Shokat
  • Il Dong Choi
  • Milica Momcilovic
  • Chao Zhang
  • Satish Pasula
  • Stefan Hohmann
  • Junro Shinoda
  • R Jiang
  • Nitnipa Soontorngun
  • Deifilia Ahuatzi
  • Jennifer A Benanti
  • Vinod Nayak
  • Kristina Hedbacker
  • Chris J C van Oevelen
  • Veerle De Wever
  • Ellen Kanter
  • Caroline Dubacq
  • Troy A A Harkness
  • K Ludin
  • Ming Chin Hou
  • Jose V Gimeno-Alcañiz
  • Catherine M Sutherland
  • Christina M Shields
  • Valmik K Vyas
  • Paula G Bertram
  • Takahiko Chimura
  • M K Shirra
  • Sandra Paiva
  • Raquel Serrano
  • Rosine Haguenauer-Tsapis
  • Olivier Vincent
  • Neide Vieira
  • V K Vyas
  • A Dagkessamanskaia
  • Maria Platara
  • Jéssica Gomes-Rezende
  • W S Lo
  • R R McCartney
  • O Vincent
  • Carina Soares-Cunha
  • Sebastien Leon
  • Margarida Casal
  • David Lara
  • Carlos Casado

Detail Information

Publications107 found, 100 shown here

  1. ncbi Hxk2 regulates the phosphorylation state of Mig1 and therefore its nucleocytoplasmic distribution
    Deifilia Ahuatzi
    Departamento de Bioquimica y Biologia Molecular, Universidad de Oviedo, Campus del Cristo, Edificio Santiago Gascon, 33006 Oviedo, Spain
    J Biol Chem 282:4485-93. 2007
    ..Our findings suggest that Snf1 interacts constitutively with the Hxk2 component of the repressor complex at high and low glucose conditions...
  2. doi The role of the Snf1 kinase in the adaptive response of Saccharomyces cerevisiae to alkaline pH stress
    Antonio Casamayor
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Spain
    Biochem J 444:39-49. 2012
    ..Certain mutants in the glucose-sensing and -response pathways, such as those lacking the Snf1 kinase, are sensitive to alkalinization...
  3. pmc Roles of two protein phosphatases, Reg1-Glc7 and Sit4, and glycogen synthesis in regulation of SNF1 protein kinase
    Amparo Ruiz
    Department of Genetics and Development, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 108:6349-54. 2011
    The SNF1 protein kinase of Saccharomyces cerevisiae is a member of the SNF1/AMP-activated protein kinase family, which is essential for metabolic control, energy homeostasis, and stress responses in eukaryotes...
  4. pmc A dual role for PP1 in shaping the Msn2-dependent transcriptional response to glucose starvation
    Veerle De Wever
    Max F Perutz Laboratories, University Departments at the Vienna Biocenter, Department of Biochemistry, University of Vienna and Ludwig Boltzmann Forschungsstelle für Biochemie, Vienna, Austria
    EMBO J 24:4115-23. 2005
    ..Apart from PKA, the protein kinase Snf1 can also directly modify one of the Msn2 phosphorylation sites (S582) and thereby repress Msn2 function...
  5. doi The pathway by which the yeast protein kinase Snf1p controls acquisition of sodium tolerance is different from that mediating glucose regulation
    Tian Ye
    Department of Cell and Molecular Biology Microbiology, University of Gothenburg, Box 462, S 40530 Göteborg, Sweden
    Microbiology 154:2814-26. 2008
    ..Snf1p appears to be part of a wider functional network than previously anticipated and the full complexity of this network remains to be elucidated...
  6. pmc Snf1p regulates Gcn5p transcriptional activity by antagonizing Spt3p
    Yang Liu
    Program in Genetics, Michigan State University, East Lansing, Michigan 48824, USA
    Genetics 184:91-105. 2010
    ..Mutating four residues in the Gcn5p catalytic domain, T203, S204, T211, and Y212 (TSTY), phenocopies snf1 null cells, including Gcn5p hypophosphorylation, hypoacetylation at the HIS3 promoter, and transcriptional defects ..
  7. pmc Roles of the Snf1-activating kinases during nitrogen limitation and pseudohyphal differentiation in Saccharomyces cerevisiae
    Marianna Orlova
    Department of Biological Sciences, University of Wisconsin Milwaukee, Milwaukee, WI 53211, USA
    Eukaryot Cell 9:208-14. 2010
    In Saccharomyces cerevisiae, Snf1 protein kinase is important for growth on carbon sources that are less preferred than glucose...
  8. pmc Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2
    Nitnipa Soontorngun
    Department of Medicine, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada H3A 1A1
    Mol Cell Biol 27:7895-905. 2007
    ..Finally, we show that upon a shift to ethanol, Rds2 activation is correlated with its hyperphosphorylation by the Snf1 kinase. In summary, we have characterized Rds2 as a novel major regulator of gluconeogenesis.
  9. ncbi Structure and dimerization of the kinase domain from yeast Snf1, a member of the Snf1/AMPK protein family
    Vinod Nayak
    The Wistar Institute, University of Pennsylvania, Philadelphia 19104, USA
    Structure 14:477-85. 2006
    The Snf1/AMPK kinases are intracellular energy sensors, and the AMPK pathway has been implicated in a variety of metabolic human disorders...
  10. doi Regulatory mechanism for expression of GPX1 in response to glucose starvation and Ca in Saccharomyces cerevisiae: involvement of Snf1 and Ras/cAMP pathway in Ca signaling
    Takumi Ohdate
    Laboratory of Molecular Microbiology, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611 0011, Japan
    Genes Cells 15:59-75. 2010
    ..The Ras/cAMP pathway is also involved in the expression of GPX1. We found that Snf1, a Ser/Thr protein kinase, is involved in the glucose starvation- and Ca(2+)-induced expression of GPX1...
  11. pmc Ubp8 and SAGA regulate Snf1 AMP kinase activity
    Marenda A Wilson
    Department of Biochemistry and Molecular Biology, Center forCancer Epigenetics, The University of Texas MD Anderson Cancer Center, 1515 Holcombe Blvd, Box 1000, Houston, Texas 77030, USA
    Mol Cell Biol 31:3126-35. 2011
    ..a proteomics approach, we determined that the SAGA complex also deubiquitinates nonhistone proteins, including Snf1, an AMP-activated kinase...
  12. pmc Access denied: Snf1 activation loop phosphorylation is controlled by availability of the phosphorylated threonine 210 to the PP1 phosphatase
    Eric M Rubenstein
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    J Biol Chem 283:222-30. 2008
    Phosphorylation of the Saccharomyces cerevisiae Snf1 kinase activation loop is determined by the integration of two reaction rates: the rate of phosphorylation by upstream kinases and the rate of dephosphorylation by Glc7...
  13. pmc Synergistic release from glucose repression by mig1 and ssn mutations in Saccharomyces cerevisiae
    L G Vallier
    Department of Genetics, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Genetics 137:49-54. 1994
    ..We report that mig1 partially restores SUC2 expression in mutants lacking the SNF1 protein kinase and show that mig1 is allelic to ssn1, a mutation selected as a suppressor of snf1...
  14. pmc Nitrogen availability and TOR regulate the Snf1 protein kinase in Saccharomyces cerevisiae
    Marianna Orlova
    Department of Biological Sciences, University of Wisconsin Milwaukee, 3209 N Maryland Ave, Milwaukee, WI 53211, USA
    Eukaryot Cell 5:1831-7. 2006
    In the yeast Saccharomyces cerevisiae, the Snf1 protein kinase of the Snf1/AMP-activated protein kinase (AMPK) family regulates a wide range of responses to stress caused by glucose deprivation...
  15. ncbi Glucose repression in yeast
    M Carlson
    Departments of Genetics and Development and Microbiology, Columbia University, HHSC 922, Box 136, 701 W 168th Street, New York, NY 10032, USA
    Curr Opin Microbiol 2:202-7. 1999
    The Snf1 protein kinase is a central component of the signaling pathway for glucose repression in yeast...
  16. ncbi Yeast SNF1 is functionally related to mammalian AMP-activated protein kinase and regulates acetyl-CoA carboxylase in vivo
    A Woods
    Medical Research Council of Molecular Medicine, Royal Postgraduate Medical School, London, United Kingdom
    J Biol Chem 269:19509-15. 1994
    The product of the SNF1 gene is a protein kinase whose activity is essential for transcriptional activation of glucose repressed genes in Saccharomyces cerevisiae...
  17. ncbi Snf1 protein kinase regulates Adr1 binding to chromatin but not transcription activation
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 277:38095-103. 2002
    ..The yeast homolog of the AMP-activated protein kinase, Snf1, promotes Adr1 chromatin binding in the absence of glucose, and the protein phosphatase complex, Glc7...
  18. pmc Convergence of TOR-nitrogen and Snf1-glucose signaling pathways onto Gln3
    Paula G Bertram
    Department of Pathology and Immunology, Washington University School of Medicine, St Louis, MO 63110, USA
    Mol Cell Biol 22:1246-52. 2002
    ..study, we show that glucose also regulates Gln3 phosphorylation and subcellular localization, which is mediated by Snf1, the yeast homolog of AMP-dependent protein kinase and a cytoplasmic glucose sensor...
  19. pmc Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation
    Sergei Kuchin
    Department of Genetics and Development, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 22:3994-4000. 2002
    The Snf1 protein kinase of Saccharomyces cerevisiae is important for many cellular responses to glucose limitation, including haploid invasive growth...
  20. ncbi Crystal structure of the heterotrimer core of Saccharomyces cerevisiae AMPK homologue SNF1
    Gabriele A Amodeo
    Department of Biological Sciences, Columbia University, New York, New York 10027, USA
    Nature 449:492-5. 2007
    ..The AMPK homologue in Saccharomyces cerevisiae, known as SNF1, is essential for responses to glucose starvation as well as for other cellular processes, although SNF1 seems to ..
  21. ncbi Elm1p is one of three upstream kinases for the Saccharomyces cerevisiae SNF1 complex
    Catherine M Sutherland
    Division of Molecular Physiology, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom
    Curr Biol 13:1299-305. 2003
    The yeast SNF1 protein kinase and the mammalian AMP-activated protein kinase are highly conserved heterotrimeric complexes that are "metabolic master switches" involved in the switch from fermentative/anaerobic to oxidative metabolism...
  22. pmc The protein kinase Snf1 is required for tolerance to the ribonucleotide reductase inhibitor hydroxyurea
    Caroline Dubacq
    Service de Biochimie et de Génétique Moléculaire, CEA Saclay, 91191 Gif sur Yvette, France
    Mol Cell Biol 24:2560-72. 2004
    The Snf1/AMP-activated kinases are involved in a wide range of stress responses in eukaryotic cells. We discovered a novel role for the Snf1 kinase in the cellular response to genotoxic stress in yeast...
  23. pmc Snf1 dependence of peroxisomal gene expression is mediated by Adr1
    Sooraj Ratnakumar
    Department of Biochemistry, University of Washington, Seattle, Washington 98195 7350, USA
    J Biol Chem 285:10703-14. 2010
    ..The AMP-activated protein kinase regulates this process in mammalian cells, and its homolog Snf1, together with the transcription factors Adr1, Oaf1, and Pip2, regulates peroxisome proliferation and beta-..
  24. pmc Histone H3 Ser10 phosphorylation-independent function of Snf1 and Reg1 proteins rescues a gcn5- mutant in HIS3 expression
    Yang Liu
    401 BCH Building, Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824
    Mol Cell Biol 25:10566-79. 2005
    ..Reg1 protein is a major regulator of Snf1 kinase that phosphorylates Ser10 of histone H3...
  25. pmc Reconstruction of the yeast Snf1 kinase regulatory network reveals its role as a global energy regulator
    Renata Usaite
    Department of Systems Biology, Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Lyngby, Denmark
    Mol Syst Biol 5:319. 2009
    ..To map the complete network of interactions around AMPK in yeast (Snf1) and to evaluate the role of its regulatory subunit Snf4, we measured global mRNA, protein and metabolite levels in ..
  26. pmc Regulatory domains of Snf1-activating kinases determine pathway specificity
    Eric M Rubenstein
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA
    Eukaryot Cell 5:620-7. 2006
    In Saccharomyces cerevisiae, the Snf1 kinase can be activated by any one of three upstream kinases, Sak1, Tos3, or Elm1...
  27. ncbi Subunits of the Snf1 kinase heterotrimer show interdependence for association and activity
    Karin Elbing
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 281:26170-80. 2006
    The Snf1 kinase and its mammalian orthologue, the AMP-activated protein kinase (AMPK), function as heterotrimers composed of a catalytic alpha-subunit and two non-catalytic subunits, beta and gamma...
  28. pmc Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongation
    Sandra Holbein
    Institute of Molecular Biology, University of Zurich, CH 8057 Zurich, Switzerland
    RNA 15:837-49. 2009
    ..Finally, chemical-genetic profiling revealed genome-wide pathways linked to cordycepin activity and identified novel genes involved in poly(A) homeostasis...
  29. pmc Regulation of the nucleocytoplasmic distribution of Snf1-Gal83 protein kinase
    Kristina Hedbacker
    Department of Genetics and Development, Columbia University, 701 W 168th Street, HSC922, New York, NY 10032, USA
    Eukaryot Cell 5:1950-6. 2006
    b>Snf1 protein kinase containing the beta subunit Gal83 is localized in the cytoplasm during growth of Saccharomyces cerevisiae cells in abundant glucose and accumulates in the nucleus in response to glucose limitation...
  30. pmc A chemical genomics study identifies Snf1 as a repressor of GCN4 translation
    Margaret K Shirra
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania 15260, USA
    J Biol Chem 283:35889-98. 2008
    The Saccharomyces cerevisiae Snf1 kinase plays a critical role in recalibrating cellular metabolism in response to glucose depletion. Hundreds of genes show changes in expression levels when the SNF1 gene is deleted...
  31. ncbi Regulation of snf1 protein kinase in response to environmental stress
    Seung Pyo Hong
    Department of Genetics and Development, Columbia University, New York, New York 10032, USA
    J Biol Chem 282:16838-45. 2007
    The Saccharomyces cerevisiae Snf1 protein kinase, a member of the Snf1/AMPK (AMP-activated protein kinase) family, has important roles in metabolic control, particularly in response to nutrient stress...
  32. pmc The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex
    R Jiang
    Department of Genetics and Development, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 17:2099-106. 1997
    The Snf1 protein kinase plays a central role in the response to glucose starvation in the yeast Saccharomyces cerevisiae...
  33. pmc A suppressor of SNF1 mutations causes constitutive high-level invertase synthesis in yeast
    M Carlson
    Genetics 107:19-32. 1984
    The SNF1 gene product of Saccharomyces cerevisiae is required to derepress expression of many glucose-repressible genes, including the SUC2 structural gene for invertase...
  34. pmc Snf1 protein kinase regulates phosphorylation of the Mig1 repressor in Saccharomyces cerevisiae
    M A Treitel
    Departments of Genetics and Development, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 18:6273-80. 1998
    ..Here we examine the role of Mig1 in regulating repression and the role of the Snf1 protein kinase in regulating Mig1 function...
  35. ncbi Regulation of Snf1 kinase. Activation requires phosphorylation of threonine 210 by an upstream kinase as well as a distinct step mediated by the Snf4 subunit
    R R McCartney
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 276:36460-6. 2001
    The yeast Snf1 kinase and its metazoan orthologues, the AMP-activated protein kinases, are activated in response to nutrient limitation...
  36. pmc Inhibition of acetyl coenzyme A carboxylase activity restores expression of the INO1 gene in a snf1 mutant strain of Saccharomyces cerevisiae
    M K Shirra
    Department of Biological Sciences, University of Pittsburgh, Pittsburgh, PA 15260, USA
    Mol Cell Biol 21:5710-22. 2001
    Mutations in the Saccharomyces cerevisiae SNF1 gene affect a number of cellular processes, including the expression of genes involved in carbon source utilization and phospholipid biosynthesis...
  37. pmc Glucose-regulated interaction of a regulatory subunit of protein phosphatase 1 with the Snf1 protein kinase in Saccharomyces cerevisiae
    K Ludin
    Departments of Genetics and Development and Microbiology, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 95:6245-50. 1998
    The Snf1 protein kinase family has been conserved in eukaryotes. In the yeast Saccharomyces cerevisiae, Snf1 is essential for transcription of glucose-repressed genes in response to glucose starvation...
  38. pmc Genes affecting the regulation of SUC2 gene expression by glucose repression in Saccharomyces cerevisiae
    L Neigeborn
    Genetics 108:845-58. 1984
    ..These mutations included five new snf1 (sucrose nonfermenting) alleles and also defined five new complementation groups, designated snf2, snf3, snf4, snf5,..
  39. ncbi Interactions between cAMP-dependent and SNF1 protein kinases in the control of glycogen accumulation in Saccharomyces cerevisiae
    T A Hardy
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis 46202 5122
    J Biol Chem 269:27907-13. 1994
    ..Mutants defective in the SNF1 gene, which encodes a protein kinase important in glucose repression mechanisms, are known not to accumulate ..
  40. ncbi Physiological characterization of glucose repression in the strains with SNF1 and SNF4 genes deleted
    Renata Usaite
    Center for Microbial Biotechnology, BioCentrum DTU, Technical University of Denmark, Building 223, DK 2800 Kgs Lyngby, Denmark
    J Biotechnol 133:73-81. 2008
    We investigated the effect of Snf1 kinase and its regulatory subunit Snf4 on the regulation of glucose and galactose metabolism in the yeast Saccharomyces cerevisiae by physiologically characterizing Deltasnf1, Deltasnf4 and ..
  41. pmc Two homologous zinc finger genes identified by multicopy suppression in a SNF1 protein kinase mutant of Saccharomyces cerevisiae
    F Estruch
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York 10032
    Mol Cell Biol 13:3872-81. 1993
    The MSN2 gene was selected as a multicopy suppressor in a temperature-sensitive SNF1 protein kinase mutant of Saccharomyces cerevisiae...
  42. pmc Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism
    O Vincent
    Departments of Genetics and Development and Microbiology, Molecular Biology and Biophysical Studies, Columbia University, New York, New York 10032, USA
    Genes Dev 15:1104-14. 2001
    The Snf1/AMP-activated protein kinase family has broad roles in transcriptional, metabolic, and developmental regulation in response to stress. In Saccharomyces cerevisiae, Snf1 is required for the response to glucose limitation...
  43. ncbi A novel genetic system to detect protein-protein interactions
    S Fields
    Department of Microbiology, State University of New York at Stony Brook, Stony Brook 11794
    Nature 340:245-6. 1989
    ..We have tested this system using two yeast proteins that are known to interact--SNF1 and SNF4. High transcriptional activity is obtained only when both hybrids are present in a cell...
  44. pmc NRG1 is required for glucose repression of the SUC2 and GAL genes of Saccharomyces cerevisiae
    H Zhou
    Department of Genetics Harvard Medical School 200 Longwood Avenue Boston, MA 02115, USA
    BMC Genet 2:5. 2001
    ..Past results suggest that other repressors may be involved in glucose repression...
  45. ncbi Rod1, an arrestin-related protein, is phosphorylated by Snf1-kinase in Saccharomyces cerevisiae
    Junro Shinoda
    Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 364:258-63. 2007
    ..We found that Rod1 interacted with Snf4, a subunit of Snf1-kinase complex...
  46. pmc Regulatory interactions between the Reg1-Glc7 protein phosphatase and the Snf1 protein kinase
    P Sanz
    Departments of Genetics and Development and Microbiology, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 20:1321-8. 2000
    ..Previous studies showed that Reg1 regulates the Snf1 protein kinase in response to glucose. Here, we explore the functional relationships between Reg1, Glc7, and Snf1...
  47. ncbi A role for the non-phosphorylated form of yeast Snf1: tolerance to toxic cations and activation of potassium transport
    Francisco Portillo
    Instituto de Investigaciones Biomedicas, Universidad Autónoma de Madrid C S I C, Arturo Duperier 4, 28029 Madrid, Spain
    FEBS Lett 579:512-6. 2005
    The Snf1/AMP-activated protein kinases play a key role in stress responses of eukaryotic cells...
  48. pmc A regulatory shortcut between the Snf1 protein kinase and RNA polymerase II holoenzyme
    S Kuchin
    Department of Genetics and Development and Department of Microbiology, Columbia University, New York, NY 10032, USA
    Proc Natl Acad Sci U S A 97:7916-20. 2000
    ..Here we present evidence for a "shortcut" mechanism in which the Snf1 protein kinase of the glucose signaling pathway directly regulates transcription by the yeast holoenzyme...
  49. pmc Interaction of the repressors Nrg1 and Nrg2 with the Snf1 protein kinase in Saccharomyces cerevisiae
    V K Vyas
    Integrated Program in Cellular Biology, Molecular Biology and Biophysical Studies, Columbia University, New York, NY 10032, USA
    Genetics 158:563-72. 2001
    The Snf1 protein kinase is essential for the transcription of glucose-repressed genes in Saccharomyces cerevisiae. We identified Nrg2 as a protein that interacts with Snf1 in the two-hybrid system...
  50. pmc A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis
    Michel Becuwe
    Institut Jacques Monod, Centre National de la Recherche Scientifique, UMR 7592, Universite Paris Diderot, Sorbonne Paris Cité, F 75205 Paris, France
    J Cell Biol 196:247-59. 2012
    ..Indeed, Rod1 was a direct target of the glucose signaling pathway composed of the AMPK homologue Snf1 and the PP1 phosphatase Glc7/Reg1...
  51. pmc Complex formation between RAS and RAF and other protein kinases
    L Van Aelst
    Cold Spring Harbor Laboratory, NY 11724
    Proc Natl Acad Sci U S A 90:6213-7. 1993
    ..We observed interaction between MEK (a kinase that phosphorylates mitogen-activated protein kinases) and the catalytic domain of RAF. RAS and MEK also interacted but only when RAF was overexpressed...
  52. pmc Gis4, a new component of the ion homeostasis system in the yeast Saccharomyces cerevisiae
    Tian Ye
    Department of Cell and Molecular Biology Microbiology, Goteborg University, Box 462, S 40530 Göteborg, Sweden
    Eukaryot Cell 5:1611-21. 2006
    ..Genetic evidence suggests that Gis4 mediates its function in salt tolerance, at least partly, together with the Snf1 protein kinase and in parallel with the calcineurin protein phosphatase...
  53. pmc Yeast SNF1 protein kinase interacts with SIP4, a C6 zinc cluster transcriptional activator: a new role for SNF1 in the glucose response
    P Lesage
    Department of Genetics and Development, Institute of Cancer Research, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA
    Mol Cell Biol 16:1921-8. 1996
    The SNF1 protein kinase has been widely conserved in plants and mammals. In Saccharomyces cerevisiae, SNF1 is essential for expression of glucose-repressed genes in response to glucose deprivation...
  54. doi Snf1-independent, glucose-resistant transcription of Adr1-dependent genes in a mediator mutant of Saccharomyces cerevisiae
    Elton T Young
    Department of Biochemistry, University of Washington, Seattle, WA, USA
    Mol Microbiol 74:364-83. 2009
    ..Adr1 is a glucose-regulated transcription factor whose promoter binding and activity require Snf1, the yeast homologue of the AMP-activated protein kinase in higher eukaryotes...
  55. pmc Biochemical evidence for glucose-independent induction of HXT expression in Saccharomyces cerevisiae
    Satish Pasula
    Mississippi Functional Genomics Network, Department of Biological Sciences, The University of Southern Mississippi, Hattiesburg, MS 39406, USA
    FEBS Lett 581:3230-4. 2007
    ..Finally, we show that active Snf1 protein kinase in high glucose prevents degradation of Mth1 and Std1.
  56. ncbi A proteomic screen reveals SCFGrr1 targets that regulate the glycolytic-gluconeogenic switch
    Jennifer A Benanti
    Department of Biochemistry and Biophysics, Cancer Research Institute, University of California, San Francisco, 2340 Sutter Street, San Francisco, CA 94115, USA
    Nat Cell Biol 9:1184-91. 2007
    ..Moreover, Pfk27 is phosphorylated by the kinase Snf1, and unphosphorylatable Pfk27 is stable and inhibits growth in the absence of glucose...
  57. pmc ADP regulates SNF1, the Saccharomyces cerevisiae homolog of AMP-activated protein kinase
    Faith V Mayer
    MRC Clinical Sciences Centre, Cellular Stress Group, Hammersmith Hospital Campus, Imperial College, DuCane Road, London, UK
    Cell Metab 14:707-14. 2011
    The SNF1 protein kinase complex plays an essential role in regulating gene expression in response to the level of extracellular glucose in budding yeast...
  58. pmc Acetylation of yeast AMPK controls intrinsic aging independently of caloric restriction
    Jin Ying Lu
    Department of Laboratory Medicine, National Taiwan University Hospital, National Taiwan University, Taipei 100, Taiwan
    Cell 146:969-79. 2011
    ..Here, we report that NuA4 acetylation of Sip2, a regulatory β subunit of the Snf1 complex (yeast AMP-activated protein kinase), decreases as cells age...
  59. pmc Roles of the glycogen-binding domain and Snf4 in glucose inhibition of SNF1 protein kinase
    Milica Momcilovic
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    J Biol Chem 283:19521-9. 2008
    The SNF1/AMP-activated protein kinase (AMPK) family is required for adaptation to metabolic stress and energy homeostasis. The gamma subunit of AMPK binds AMP and ATP, and mutations that affect binding cause human disease...
  60. pmc Differential roles of the glycogen-binding domains of beta subunits in regulation of the Snf1 kinase complex
    Simmanjeet Mangat
    Biochemistry and Molecular Genetics Program, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Eukaryot Cell 9:173-83. 2010
    Members of the AMP-activated protein kinase family, including the Snf1 kinase of Saccharomyces cerevisiae, are activated under conditions of nutrient stress...
  61. pmc An inhibited conformation for the protein kinase domain of the Saccharomyces cerevisiae AMPK homolog Snf1
    Michael J Rudolph
    Department of Biological Sciences, Columbia University, New York, NY 10027, USA
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:999-1002. 2010
    ..Its homolog in yeast, SNF1, is activated in response to glucose depletion and other stresses...
  62. pmc Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II
    C L Denis
    Genetics 108:833-44. 1984
    ..effect on the repression of ADHII when it was combined with the ADR1-5c allele, whereas the phenotypically similar ccr1 allele, which partially suppresses ADR1-5c, did not suppress the cre1 or cre2 phenotype...
  63. pmc Histone H3 phosphorylation can promote TBP recruitment through distinct promoter-specific mechanisms
    Wan Sheng Lo
    Division of Biological Sciences and UCSD Cancer Center, University of California, San Diego, CA, USA
    EMBO J 24:997-1008. 2005
    ..Previously, we showed that histone H3 Ser10 phosphorylation (pSer10) by the yeast Snf1 kinase regulates INO1 gene activation in part via Gcn5/SAGA complex-mediated Lys14 acetylation (acLys14)...
  64. pmc The Hsp70 homolog Ssb is essential for glucose sensing via the SNF1 kinase network
    Ulrike von Plehwe
    Institute of Biochemistry and Molecular Biology, ZBMZ, University of Freiburg, D 79104 Freiburg, Germany
    Genes Dev 23:2102-15. 2009
    ..One of the central components is SNF1, the homolog of mammalian AMP-activated protein kinase, which in yeast is essential for the expression of glucose-..
  65. pmc Relationship of the cAMP-dependent protein kinase pathway to the SNF1 protein kinase and invertase expression in Saccharomyces cerevisiae
    E J Hubbard
    Department of Genetics, Columbia University College of Physicians and Surgeons, New York, New York 10032
    Genetics 130:71-80. 1992
    The SNF1 protein kinase and the associated SNF4 protein are required for release of glucose repression in Saccharomyces cerevisiae...
  66. pmc The Kap60-Kap95 karyopherin complex directly regulates phosphatidylcholine synthesis
    Melissa A MacKinnon
    Department of Pediatrics, Atlantic Research Centre, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
    J Biol Chem 284:7376-84. 2009
    ..Diminution of Kap95 function resulted in almost complete ablation of phosphatidylcholine synthesis under conditions where Pct1 was extranuclear. The beta-importin Kap95 is a direct regulator membrane synthesis...
  67. ncbi Genetic and carbon source regulation of phosphorylation of Sip1p, a Snf1p-associated protein involved in carbon response in Saccharomyces cerevisiae
    R M Long
    Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey 17033, USA
    Yeast 11:233-46. 1995
    ..The Sip1 protein is known to undergo phosphorylation when associated in vitro with the Snf1 protein kinase. We have carried out in vivo studies of the genetic and carbon control of Sip1p phosphorylation...
  68. pmc CAT8, a new zinc cluster-encoding gene necessary for derepression of gluconeogenic enzymes in the yeast Saccharomyces cerevisiae
    D Hedges
    Institut für Mikrobiologie der Johann Wolfgang Goethe Universität Frankfurt, Germany
    Mol Cell Biol 15:1915-22. 1995
    ..This gene activation depended on the previously identified derepression genes CAT1 (SNF1) (encoding a protein kinase) and CAT3 (SNF4) (probably encoding a subunit of Cat1p [Snf1p])...
  69. pmc Characterization of an essential Orc2p-associated factor that plays a role in DNA replication
    C F Hardy
    Department of Cell Biology and Physiology, Washington University School of Medicine, St Louis, Missouri 63110, USA
    Mol Cell Biol 16:1832-41. 1996
    ..These results suggest a role for OAFI in the initiation of DNA replication. Mutant alleles of cdc7 and cdc14 were also isolated in the orc2-1 synthetic lethal screen. Cdc7p, like Oaf1p, also interacts with Orc2p in two-hybrid assays...
  70. ncbi Snf1p-dependent Spt-Ada-Gcn5-acetyltransferase (SAGA) recruitment and chromatin remodeling activities on the HXT2 and HXT4 promoters
    Chris J C van Oevelen
    Department of Physiological Chemistry, Division of Biomedical Genetics, University Medical Center Utrecht, The Netherlands
    J Biol Chem 281:4523-31. 2006
    ..In contrast, histone H3 removal from the HXT2 promoter does not require Gcn5p. In conclusion, although similar protein complexes are involved, induction of HXT2 and HXT4 displays important mechanistic differences...
  71. ncbi Purification and characterization of Snf1 kinase complexes containing a defined Beta subunit composition
    Nandita Nath
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    J Biol Chem 277:50403-8. 2002
    The Snf1 kinase complex of Saccharomyces cerevisiae contains one of three possible beta subunits encoded by either SIP1, SIP2, or GAL83...
  72. pmc Interactions within the yeast Sm core complex: from proteins to amino acids
    A Camasses
    CNRS UPR 9005, Strasbourg, France
    Mol Cell Biol 18:1956-66. 1998
    ..Surprisingly, we observed that other evolutionarily conserved positions are tolerant to mutations, with substitutions affecting binding to SmF and SmG only mildly and conferring a wild-type growth phenotype...
  73. ncbi Mob2p interacts with the protein kinase Orb6p to promote coordination of cell polarity with cell cycle progression
    Ming Chin Hou
    Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01605, USA
    J Cell Sci 116:125-35. 2003
    ..Our results suggest that Mob2p, along with Orb6p, is required for coordinating polarized cell growth during interphase with the onset of mitosis...
  74. pmc Isolation of mutations in the catalytic domain of the snf1 kinase that render its activity independent of the snf4 subunit
    Anna Leech
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Eukaryot Cell 2:265-73. 2003
    Activation of the Snf1 kinase requires at least two events, phosphorylation of the activation loop on threonine 210 and an Snf4-dependent process that is not completely defined...
  75. pmc Yeast Pak1 kinase associates with and activates Snf1
    Nandita Nath
    Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania 15261, USA
    Mol Cell Biol 23:3909-17. 2003
    Members of the Snf1/AMP-activated protein kinase family are activated under conditions of nutrient stress by a distinct upstream kinase. Here we present evidence that the yeast Pak1 kinase functions as a Snf1-activating kinase...
  76. pmc Antagonistic controls of autophagy and glycogen accumulation by Snf1p, the yeast homolog of AMP-activated protein kinase, and the cyclin-dependent kinase Pho85p
    Z Wang
    Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202, USA
    Mol Cell Biol 21:5742-52. 2001
    ..Yeast mutated in SNF1, a gene encoding a protein kinase required for glucose derepression, has diminished glycogen accumulation and ..
  77. ncbi Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis
    Christina M Shields
    School of Biological Sciences, University of Nebraska Lincoln, Lincoln, NE 68588 0666, USA
    Curr Genet 44:184-94. 2003
    ..Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p...
  78. ncbi Genetics of carbon catabolite repression in Saccharomycess cerevisiae: genes involved in the derepression process
    F K Zimmermann
    Mol Gen Genet 151:95-103. 1977
    A recessive mutant cat1-1, wild type CAT1, was isolated in Saccharomyces cerevisiae. It did not grow on glycerol nor ferment maltose even with fully constitutive, glucose resistant maltase synthesis...
  79. ncbi Glucose and type 2A protein phosphatase regulate the interaction between catalytic and regulatory subunits of AMP-activated protein kinase
    Jose V Gimeno-Alcañiz
    Instituto de Biomedicina de Valencia CSIC, Jaime Roig 11, E 46010 Valencia, Spain
    J Mol Biol 333:201-9. 2003
    ..This regulation was not affected by known regulators of the corresponding yeast orthologue, the SNF1 complex, such as Reg1 or Hxk2, but it was affected by deletion of regulatory subunits of yeast type 2A protein ..
  80. ncbi Interaction of Knr4 protein, a protein involved in cell wall synthesis, with tyrosine tRNA synthetase encoded by TYS1 in Saccharomyces cerevisiae
    A Dagkessamanskaia
    Centre de Bioingenierie Gilbert Durand, UMR CNRS 5504, UMR INRA 792, Toulouse, France
    FEMS Microbiol Lett 200:53-8. 2001
    ..Altogether, these data provide novel functions of Knr4p and Tys1p to those that were known before...
  81. pmc Aip1p interacts with cofilin to disassemble actin filaments
    A A Rodal
    Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720, USA
    J Cell Biol 145:1251-64. 1999
    ..We conclude that Aip1p is a cofilin-associated protein that enhances the filament disassembly activity of cofilin and restricts cofilin localization to cortical actin patches...
  82. pmc Architecture of coatomer: molecular characterization of delta-COP and protein interactions within the complex
    D Faulstich
    Institut fur Biochemie I, Heidelberg, Germany
    J Cell Biol 135:53-61. 1996
    ..We propose that these interactions reflect in vivo associations of those subunits and thus play a functional role in the assembly of coatomer and/or serve to maintain the molecular architecture of the complex...
  83. ncbi Yeast genes GIS1-4: multicopy suppressors of the Gal- phenotype of snf1 mig1 srb8/10/11 cells
    D Balciunas
    Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala Genetic Center
    Mol Gen Genet 262:589-99. 1999
    ..in budding yeast causes a reduction in expression of the GAL genes, which is particularly pronounced in a mig1 snf1 background...
  84. pmc Cooperative regulation of DOG2, encoding 2-deoxyglucose-6-phosphate phosphatase, by Snf1 kinase and the high-osmolarity glycerol-mitogen-activated protein kinase cascade in stress responses of Saccharomyces cerevisiae
    Y Tsujimoto
    Research Institute for Food Science, Kyoto University, Uji, Japan
    J Bacteriol 182:5121-6. 2000
    ..These results suggest that Snf1p kinase and the high-osmolarity glycerol-mitogen-activated protein kinase cascade are likely to be involved in the signaling pathway of oxidative stress and osmotic stress in regulation of DOG2...
  85. pmc Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases
    R P Bhalerao
    Max Planck Institut fur Zuchtungsforschung, D 50829 Cologne, Germany
    Proc Natl Acad Sci U S A 96:5322-7. 1999
    ..The yeast SNF1 protein kinase, a key regulator of glucose signaling, and Arabidopsis SNF1 homologs AKIN10 and AKIN11, which can ..
  86. pmc A functional analysis reveals dependence on the anaphase-promoting complex for prolonged life span in yeast
    Troy A A Harkness
    Department of Anatomy and Cell Biology, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E5, Canada
    Genetics 168:759-74. 2004
    ..Therefore, we propose that APC-dependent genomic stability is linked to prolonged longevity by the antagonistic regulation of the PKA and Snf1p pathways...
  87. ncbi A protein kinase substrate identified by the two-hybrid system
    X Yang
    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, NY 10032
    Science 257:680-2. 1992
    A genetic method, the two-hybrid system, was used to identify four genes encoding proteins that interact with the SNF1 protein kinase from yeast...
  88. ncbi The yeast cyclins Pc16p and Pc17p are involved in the control of glycogen storage by the cyclin-dependent protein kinase Pho85p
    Z Wang
    Department of Biochemistry and Molecular Biology and Center for Diabetes Research, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202 5122, USA
    FEBS Lett 506:277-80. 2001
    ..polysaccharide glycogen under certain conditions and deletion of PCL6 and PCL7 restores glycogen accumulation to a snf1 pcl8 pcl10 triple mutant, paradoxically activating both glycogen synthase and phosphorylase...
  89. pmc Identification and characterization of CIA/ASF1 as an interactor of bromodomains associated with TFIID
    Takahiko Chimura
    Laboratory of Developmental Biology, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 0032, Japan
    Proc Natl Acad Sci U S A 99:9334-9. 2002
    ..This study describes the functional linkage between TFIID and a histone chaperone...
  90. pmc Mutational analysis of the Saccharomyces cerevisiae SNF1 protein kinase and evidence for functional interaction with the SNF4 protein
    J L Celenza
    Department of Genetics and Development, College of Physicians and Surgeons of Columbia University, New York, New York 10032
    Mol Cell Biol 9:5034-44. 1989
    The SNF1 gene of Saccharomyces cerevisiae encodes a protein-serine/threonine kinase that is required for derepression of gene expression in response to glucose limitation...
  91. pmc Detection of protein-protein interactions through vesicle targeting
    Jacob H Boysen
    Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA
    Genetics 182:33-9. 2009
    ..cerevisiae and the fungal pathogen Candida albicans. We use computational analysis of microscopic images to provide a quantitative and automated assessment of confidence...
  92. pmc Dual influence of the yeast Cat1p (Snf1p) protein kinase on carbon source-dependent transcriptional activation of gluconeogenic genes by the regulatory gene CAT8
    A Rahner
    Institut fur Mikrobiologie, Biochemie und Genetik, Lehrstuhl Biochemie, Universitat Erlangen Nurnberg, Erlangen, Germany
    Nucleic Acids Res 24:2331-7. 1996
    ..The derepression defect of CAT8 in a cat1 mutant could be suppressed by a mutant Mig1p repressor protein...
  93. pmc Glucose depletion causes haploid invasive growth in yeast
    P J Cullen
    Institute of Molecular Biology, University of Oregon, Eugene, OR 97403 1229, USA
    Proc Natl Acad Sci U S A 97:13619-24. 2000
    ..We tested glucose control proteins for a role in invasion and found that Snf1, a protein required for derepression of glucose-repressed genes, was required for invasive growth...
  94. pmc The Snf1 protein kinase and Sit4 protein phosphatase have opposing functions in regulating TATA-binding protein association with the Saccharomyces cerevisiae INO1 promoter
    Margaret K Shirra
    Department of Biological Sciences, University of Pittsburgh, Pennsylvania 15260, USA
    Genetics 169:1957-72. 2005
    ..cerevisiae INO1 gene. Full activation of INO1 transcription occurs in the absence of inositol and requires the Snf1 protein kinase in addition to other signaling molecules and transcription factors...
  95. pmc Analysis of the SIP3 protein identified in a two-hybrid screen for interaction with the SNF1 protein kinase
    P Lesage
    Department of Genetics and Development, Columbia University, College of Physicians and Surgeons, New York, NY 10032
    Nucleic Acids Res 22:597-603. 1994
    ..cerevisiae SIP3 gene was identified in a two-hybrid screen for proteins that interact in vivo with the SNF1 protein kinase, which is necessary for release of glucose repression...
  96. pmc Stimulation of yeast meiotic gene expression by the glucose-repressible protein kinase Rim15p
    S Vidan
    Department of Microbiology, Columbia University, New York, New York 10032, USA
    Mol Cell Biol 17:2688-97. 1997
    ..Glucose inhibits Ime1p-Ume6p interaction, and we find that Rim15p accumulation is repressed in glucose-grown cells. Thus, glucose repression of Rim15p may be responsible for glucose inhibition of Ime1p-Ume6p interaction...
  97. pmc CAT5, a new gene necessary for derepression of gluconeogenic enzymes in Saccharomyces cerevisiae
    M Proft
    Institut für Mikrobiologie der Johann Wolfgang Goethe Universität Frankfurt, Frankfurt am Main, Germany
    EMBO J 14:6116-26. 1995
    ..CAT5 expression is 5- to 6-fold repressed by glucose, and CAT5 transcriptional activation was dependent on CAT1 (SNF1), CAT8 and CAT5 itself...
  98. pmc Deletion of the gene encoding the cyclin-dependent protein kinase Pho85 alters glycogen metabolism in Saccharomyces cerevisiae
    B K Timblin
    Department of Microbiology and Immunology, Medical College of Pennsylvania, Philadelphia 19102 1192, USA
    Genetics 143:57-66. 1996
    ....
  99. pmc SSN genes that affect transcriptional repression in Saccharomyces cerevisiae encode SIN4, ROX3, and SRB proteins associated with RNA polymerase II
    W Song
    Department of Genetics, Columbia University, College of Physicians and Surgeons, New York, New York 10032, USA
    Mol Cell Biol 16:115-20. 1996
    ..We also show that SSN4 and SSN7 are the same as SIN4 and ROX3, respectively, raising the possibility that these genes also encode mediator proteins...
  100. pmc Sip5 interacts with both the Reg1/Glc7 protein phosphatase and the Snf1 protein kinase of Saccharomyces cerevisiae
    P Sanz
    Departments of Genetics and Development and Microbiology, Columbia University, New York, New York 10032, USA
    Genetics 154:99-107. 2000
    The Snf1 protein kinase is an essential component of the glucose starvation signalling pathway in Saccharomyces cerevisiae...
  101. ncbi The SNF1 kinase complex from Saccharomyces cerevisiae phosphorylates the transcriptional repressor protein Mig1p in vitro at four sites within or near regulatory domain 1
    F C Smith
    MRC Clinical Sciences Centre, Imperial College School of Medicine, Hammersmith Hospital, London, UK
    FEBS Lett 453:219-23. 1999
    ..On removal of medium glucose, gene repression is relieved via a mechanism that requires the SNF1 protein kinase complex...

Research Grants56

  1. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    ELTON YOUNG; Fiscal Year: 2009
    ....
  2. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    Elton T Young; Fiscal Year: 2010
    ..To activate these pathways an intricate intracellular signaling cascade is initiated by the protein kinase Snf1 and its accessory proteins...
  3. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    ELTON YOUNG; Fiscal Year: 2003
    ..Thus, the proposed specific aims relate to these aspects of the structure and function of Adr1p. ..
  4. Transcriptional Integration of Metabolism
    ELTON YOUNG; Fiscal Year: 2007
    ..b>Snf1 coordinates the expression and activity of transcription factors and other regulatory proteins that modulate the ..
  5. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    ELTON YOUNG; Fiscal Year: 1980
    ..The effect of various mutations on the levels of ADH mRNA and proteins will be characterized in an effort to understand the regulation of ADH gene expression. ..
  6. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    ELTON YOUNG; Fiscal Year: 1993
    ..Since several human diseases have been identified that are due to mutations in zinc finger transcription factors, understanding how these factors function is useful to an understanding of the defect in these diseases...
  7. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    ELTON YOUNG; Fiscal Year: 1991
    ..A basic understanding of how proteins control gene action will ultimately be of medical benefit as well...
  8. GENETIC REGULATION OF ALCOHOL METABOLISM IN YEAST
    ELTON YOUNG; Fiscal Year: 1999
    ..Thus, our specific aims relate to these aspects of the structure and function of Adr1p. ..
  9. Yeast Genetic Selection for Human AMPK Kinases
    Marian Carlson; Fiscal Year: 2006
    ..Our studies of the upstream kinases of the yeast (Saccharomyces cerevisiae) ortholog of AMPK, Snf1 protein kinase, led us to identify the LKB1 tumor suppressor kinase as the major AMPKK in liver...
  10. Regulation of Snf1/AMPK Pathway in Yeast
    Marian Carlson; Fiscal Year: 2007
    b>Snf1 protein kinase of Saccharomyces cerevisiae is a member of the highly conserved Snf1/AMP-activated protein kinase (AMPK) family, which plays a central role in responses to metabolic stress...
  11. Regulation of Snf1/AMPK Pathway in Yeast
    Marian Carlson; Fiscal Year: 2009
    b>Snf1 protein kinase of Saccharomyces cerevisiae is a member of the highly conserved Snf1/AMP-activated protein kinase (AMPK) family, which plays a central role in responses to metabolic stress...
  12. HISTONE ACETYLTRANSFERASE AND TRANSCRIPTIONAL REGULATION
    Min Hao Kuo; Fiscal Year: 2005
    ..Moreover, individual roles played by the ADA and SAGA complexes in HIS3 expression are to be investigated both genetically and biochemically. ..
  13. SNF/SWI COMPLEX AND TRANSCRIPTIONAL ACTIVATION
    Marian Carlson; Fiscal Year: 2000
    ....
  14. Role of RNA Polymerase II Holoenzyme in Regulation
    Marian Carlson; Fiscal Year: 2004
    ..The proposed studies will further our understanding of the functional interactions of RNA polymerase II holoenzymes with regulatory proteins and signal transduction pathways. ..