SNC1

Summary

Gene Symbol: SNC1
Description: SNAP receptor SNC1
Alias: SNAP receptor SNC1
Species: Saccharomyces cerevisiae S288c
Products:     SNC1

Top Publications

  1. Strop P, Kaiser S, Vrljic M, Brunger A. The structure of the yeast plasma membrane SNARE complex reveals destabilizing water-filled cavities. J Biol Chem. 2008;283:1113-9 pubmed
    ..Mutagenesis experiments suggest that the water-filled cavities contribute to the lower stability of the S. cerevisiae complex. ..
  2. Hettema E, Lewis M, Black M, Pelham H. Retromer and the sorting nexins Snx4/41/42 mediate distinct retrieval pathways from yeast endosomes. EMBO J. 2003;22:548-57 pubmed
    ..Our findings suggest a general role for yeast sorting nexins in protein retrieval, rather than degradation, and indicate that different sorting nexins operate in different classes of endosomes. ..
  3. Lehman K, Rossi G, Adamo J, Brennwald P. Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. J Cell Biol. 1999;146:125-40 pubmed
    ..Taken together, our studies suggest that members of the lethal giant larvae/tomosyn/Sro7 family play an important role in polarized exocytosis by regulating SNARE function on the plasma membrane. ..
  4. Kama R, Robinson M, Gerst J. Btn2, a Hook1 ortholog and potential Batten disease-related protein, mediates late endosome-Golgi protein sorting in yeast. Mol Cell Biol. 2007;27:605-21 pubmed
    ..We isolated Btn2 as a Snc1 v-SNARE binding protein using the two-hybrid assay and examined its role in intracellular protein trafficking...
  5. Katz L, Hanson P, Heuser J, Brennwald P. Genetic and morphological analyses reveal a critical interaction between the C-termini of two SNARE proteins and a parallel four helical arrangement for the exocytic SNARE complex. EMBO J. 1998;17:6200-9 pubmed
    ..This provides strong evidence that the exocytic SNARE complex is a highly conserved structure composed of four parallel helical domains whose C-termini must converge in order to bring about membrane fusion. ..
  6. Lewis M, Nichols B, Prescianotto Baschong C, Riezman H, Pelham H. Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes. Mol Biol Cell. 2000;11:23-38 pubmed
    ..We propose that these represent early endosomes in which sorting of Snc1p and late Golgi proteins occurs, and that transport can occur directly from them to the Golgi apparatus. ..
  7. Protopopov V, Govindan B, Novick P, Gerst J. Homologs of the synaptobrevin/VAMP family of synaptic vesicle proteins function on the late secretory pathway in S. cerevisiae. Cell. 1993;74:855-61 pubmed
    The yeast S. cerevisiae possesses two genes, SNC1 and SNC2, that encode homologs of vertebrate synaptic vesicle-associated membrane proteins, also known as synaptobrevins...
  8. Castillo Flores A, Weinberger A, Robinson M, Gerst J. Mso1 is a novel component of the yeast exocytic SNARE complex. J Biol Chem. 2005;280:34033-41 pubmed
    The yeast exocytic SNARE complex consists of one molecule each of the Sso1/2 target SNAREs, Snc1/2 vesicular SNAREs, and the Sec9 target SNARE, which form a fusion complex that is conserved in evolution...
  9. Couve A, Gerst J. Yeast Snc proteins complex with Sec9. Functional interactions between putative SNARE proteins. J Biol Chem. 1994;269:23391-4 pubmed
    ..We have previously described the yeast homologs, Snc1 and Snc2, and demonstrated that they localize to secretory vesicles and are required for normal secretion...

More Information

Publications48

  1. Grote E, Vlacich G, Pypaert M, Novick P. A snc1 endocytosis mutant: phenotypic analysis and suppression by overproduction of dihydrosphingosine phosphate lyase. Mol Biol Cell. 2000;11:4051-65 pubmed
    ..b>snc1-M43A mutant yeast have reduced growth and secretion rates and accumulate post-Golgi secretory vesicles and ..
  2. Lustgarten V, Gerst J. Yeast VSM1 encodes a v-SNARE binding protein that may act as a negative regulator of constitutive exocytosis. Mol Cell Biol. 1999;19:4480-94 pubmed
    ..designated Vsm1, binds tightly to the Snc2 v-SNARE in the two-hybrid system and can be coimmunoprecipitated with Snc1 or Snc2 from solubilized yeast cell extracts...
  3. Knop M, Miller K, Mazza M, Feng D, Weber M, Keränen S, et al. Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast. Mol Biol Cell. 2005;16:4543-56 pubmed
    ..Mso1p coimmunoprecipitates with Sec1p, Sso1/2p, Snc1/2p, Sec9p, and the exocyst complex subunit Sec15p...
  4. Marash M, Gerst J. Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast. Mol Biol Cell. 2003;14:3114-25 pubmed
    ..Thus, one way by which phosphorylation inhibits SNARE assembly could be by regulating the association of inhibitory factors that control the ability of t-SNAREs to form complexes in vivo. ..
  5. Gerst J. Conserved alpha-helical segments on yeast homologs of the synaptobrevin/VAMP family of v-SNAREs mediate exocytic function. J Biol Chem. 1997;272:16591-8 pubmed
    ..The yeast homologs, Snc1 and Snc2, localize to secretory vesicles and are required for normal bulk secretion in Saccharomyces cerevisiae...
  6. Rossi G, Salminen A, Rice L, Brunger A, Brennwald P. Analysis of a yeast SNARE complex reveals remarkable similarity to the neuronal SNARE complex and a novel function for the C terminus of the SNAP-25 homolog, Sec9. J Biol Chem. 1997;272:16610-7 pubmed
    ..characterization of a nonneuronal SNARE complex using recombinant forms of the yeast exocytic SNARE proteins Snc1, Sso1, and Sec9 and the yeast alpha-SNAP homolog, Sec17...
  7. David D, Sundarababu S, Gerst J. Involvement of long chain fatty acid elongation in the trafficking of secretory vesicles in yeast. J Cell Biol. 1998;143:1167-82 pubmed
  8. McNew J, Parlati F, Fukuda R, Johnston R, Paz K, Paumet F, et al. Compartmental specificity of cellular membrane fusion encoded in SNARE proteins. Nature. 2000;407:153-9 pubmed
    ..Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis. ..
  9. Grote E, Carr C, Novick P. Ordering the final events in yeast exocytosis. J Cell Biol. 2000;151:439-52 pubmed
    ..In the sec18-1 mutant, cis-SNARE complexes containing surface-accessible Sncp accumulate in the plasma membrane. Thus, one function of Sec18p is to disassemble SNARE complexes on the postfusion membrane. ..
  10. Brennwald P, Kearns B, Champion K, Keranen S, Bankaitis V, Novick P. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 1994;79:245-58 pubmed
    ..Our results identify Sec9 as the yeast cognate of SNAP-25 and suggest that SNARE complexes acting at specific stages of vesicular transport serve as the ultimate targets of regulation by members of the Sec4/Ypt1/Rab family of GTPases. ..
  11. Jantti J, Aalto M, Oyen M, Sundqvist L, Keränen S, Ronne H. Characterization of temperature-sensitive mutations in the yeast syntaxin 1 homologues Sso1p and Sso2p, and evidence of a distinct function for Sso1p in sporulation. J Cell Sci. 2002;115:409-20 pubmed
    ..for high copy number suppressors of sso2-1 yielded three genes that are involved in the terminal step of secretion: SNC1, SNC2 and SEC9...
  12. Gurunathan S, Chapman Shimshoni D, Trajkovic S, Gerst J. Yeast exocytic v-SNAREs confer endocytosis. Mol Biol Cell. 2000;11:3629-43 pubmed
    In yeast, homologues of the synaptobrevin/VAMP family of v-SNAREs (Snc1 and Snc2) confer the docking and fusion of secretory vesicles at the cell surface...
  13. Peng R, Gallwitz D. Sly1 protein bound to Golgi syntaxin Sed5p allows assembly and contributes to specificity of SNARE fusion complexes. J Cell Biol. 2002;157:645-55 pubmed
    ..This indicates for the first time that a Sec1 family member contributes to the specificity of SNARE complex assembly. ..
  14. Gerst J, Rodgers L, Riggs M, Wigler M. SNC1, a yeast homolog of the synaptic vesicle-associated membrane protein/synaptobrevin gene family: genetic interactions with the RAS and CAP genes. Proc Natl Acad Sci U S A. 1992;89:4338-42 pubmed
    b>SNC1, a gene from the yeast Saccharomyces cerevisiae, encodes a homolog of vertebrate synaptic vesicle-associated membrane proteins (VAMPs) or synaptobrevins. SNC1 was isolated by its ability to suppress the loss of CAP function in S...
  15. Gurunathan S, David D, Gerst J. Dynamin and clathrin are required for the biogenesis of a distinct class of secretory vesicles in yeast. EMBO J. 2002;21:602-14 pubmed
    ..In late-acting secretory mutants (e.g. snc1(ala43) and sec6-4), both low- (LDSV) and high-density (HDSV) classes of vesicles accumulate at restrictive ..
  16. Zendeh Boodi Z, Yamamoto T, Sakane H, Tanaka K. Identification of a second amphipathic lipid-packing sensor-like motif that contributes to Gcs1p function in the early endosome-to-TGN pathway. J Biochem. 2013;153:573-87 pubmed publisher
    ..These results suggest that ALPS and the predicted AH region are involved in the regulation and function of Gcs1p by interacting with membrane phospholipids and vesicle proteins. ..
  17. Schindler C, Spang A. Interaction of SNAREs with ArfGAPs precedes recruitment of Sec18p/NSF. Mol Biol Cell. 2007;18:2852-63 pubmed
  18. Lefebvre F, Prouzet Mauleon V, Hugues M, Crouzet M, Vieillemard A, McCusker D, et al. Secretory pathway-dependent localization of the Saccharomyces cerevisiae Rho GTPase-activating protein Rgd1p at growth sites. Eukaryot Cell. 2012;11:590-600 pubmed publisher
    ..Our data indicate that secretory vesicles are involved in the delivery of RhoGAP Rgd1p to the bud tip and bud neck. ..
  19. Nozawa M, Takahashi T, Hara S, Mizoguchi H. A role of Saccharomyces cerevisiae fatty acid activation protein 4 in palmitoyl-CoA pool for growth in the presence of ethanol. J Biosci Bioeng. 2002;93:288-95 pubmed
    ..The overexpression of SNC1 was used to achieve the depletion of the palmitoyl-CoA pool, because the loss of Snc palmitoylation does not affect ..
  20. Fiebig K, Rice L, Pollock E, Brunger A. Folding intermediates of SNARE complex assembly. Nat Struct Biol. 1999;6:117-23 pubmed
    ..conformation of Sso1; second, the binary complex of Sso1 and Sec9; and third, the ternary complex of Sso1, Sec9 and Snc1. Sec9 and Snc1 are unstructured in isolation...
  21. Forsmark A, Rossi G, Wadskog I, Brennwald P, Warringer J, Adler L. Quantitative proteomics of yeast post-Golgi vesicles reveals a discriminating role for Sro7p in protein secretion. Traffic. 2011;12:740-53 pubmed publisher
  22. Nair U, Jotwani A, Geng J, Gammoh N, Richerson D, Yen W, et al. SNARE proteins are required for macroautophagy. Cell. 2011;146:290-302 pubmed publisher
    ..Thus, multiple SNARE-mediated fusion events are likely to be involved in autophagosome biogenesis. ..
  23. Chen S, Chen S, Tokarev A, Liu F, Jedd G, Segev N. Ypt31/32 GTPases and their novel F-box effector protein Rcy1 regulate protein recycling. Mol Biol Cell. 2005;16:178-92 pubmed
    ..We show that like Rcy1, Ypt31/32 regulate the coupled phosphorylation and recycling of the plasma membrane v-SNARE Snc1. Moreover, Ypt31/32 and Rcy1 regulate the recycling of the furin-homolog Kex2 to the Golgi...
  24. Weber Boyvat M, Aro N, Chernov K, Nyman T, Jantti J. Sec1p and Mso1p C-terminal tails cooperate with the SNAREs and Sec4p in polarized exocytosis. Mol Biol Cell. 2011;22:230-44 pubmed publisher
    ..This association depends on the Sec4p guanine nucleotide exchange factor Sec2p. Our results reveal a novel binding mode between the Sec1p C-terminal tail and the SNARE complex, and suggest a role for Mso1p as an effector of Sec4p. ..
  25. Paumet F, Rahimian V, Rothman J. The specificity of SNARE-dependent fusion is encoded in the SNARE motif. Proc Natl Acad Sci U S A. 2004;101:3376-80 pubmed
    ..Studying this complex and the previously identified early endosomal SNARE complex, we find that the specificity of fusion resides in the SNARE motifs. ..
  26. Lewis M, Pelham H. A new yeast endosomal SNARE related to mammalian syntaxin 8. Traffic. 2002;3:922-9 pubmed
    ..Syn8p thus appears to be a functional homolog of mammalian syntaxin 8, but Tlg1p can, amongst other roles, provide an equivalent function. ..
  27. Boyd A, Ciufo L, Barclay J, Graham M, Haynes L, Doherty M, et al. A random mutagenesis approach to isolate dominant-negative yeast sec1 mutants reveals a functional role for domain 3a in yeast and mammalian Sec1/Munc18 proteins. Genetics. 2008;180:165-78 pubmed publisher
    ..This effect of dominant-negative sec1 was suppressed by overexpression of the vesicle (v)-SNAREs, Snc1 and Snc2, but not the target (t)-SNAREs, Sec9 and Sso2...
  28. Chen S, Shah A, Segev N. Ypt31/32 GTPases and their F-Box effector Rcy1 regulate ubiquitination of recycling proteins. Cell Logist. 2011;1:21-31 pubmed
    ..Specifically, both Ypt31/32 and Rcy1 are required for recycling the vSNARE Snc1. Rcy1 contains an F-box domain shared by proteins that act in substrate recognition of ubiquitin ligases...
  29. Gurunathan S, Marash M, Weinberger A, Gerst J. t-SNARE phosphorylation regulates endocytosis in yeast. Mol Biol Cell. 2002;13:1594-607 pubmed
    ..phosphatase (CAPP) conferred normal growth and secretion to yeast lacking their complement of exocytic v-SNAREs (Snc1,2) or bearing a temperature-sensitive mutation in an exocytic t-SNARE (Sso2)...
  30. Kanneganti V, Kama R, Gerst J. Btn3 is a negative regulator of Btn2-mediated endosomal protein trafficking and prion curing in yeast. Mol Biol Cell. 2011;22:1648-63 pubmed publisher
    ..Third, Btn3 and the Snc1 v-SNARE compete for the same binding domain on Btn2, and this competition controls Btn2 localization and function...
  31. Morishita M, Mendonsa R, Wright J, Engebrecht J. Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways. Traffic. 2007;8:1231-45 pubmed
    ..Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation. ..
  32. Sclafani A, Chen S, Rivera Molina F, Reinisch K, Novick P, Ferro Novick S. Establishing a role for the GTPase Ypt1p at the late Golgi. Traffic. 2010;11:520-32 pubmed publisher
    ..We propose that Ypt1p regulates the flow of traffic into the cis and trans side of the Golgi via multiple effectors. ..
  33. Grote E, Baba M, Ohsumi Y, Novick P. Geranylgeranylated SNAREs are dominant inhibitors of membrane fusion. J Cell Biol. 2000;151:453-66 pubmed
    ..High-level expression of mutant Snc1 or Sso2 proteins that have a COOH-terminal geranylgeranylation signal instead of a transmembrane domain inhibits ..
  34. Van Zyl J, Den Haan R, van Zyl W. Over-expression of native Saccharomyces cerevisiae exocytic SNARE genes increased heterologous cellulase secretion. Appl Microbiol Biotechnol. 2014;98:5567-78 pubmed publisher
    ..Over-expression of SNC1 yielded the biggest improvement in Te-Cel7A secretion (71 %), whilst SSO1 over-expression lead to the highest ..
  35. Holthuis J, Nichols B, Dhruvakumar S, Pelham H. Two syntaxin homologues in the TGN/endosomal system of yeast. EMBO J. 1998;17:113-26 pubmed
    ..However, neither is required for intra-Golgi traffic. Since no further syntaxins have been identified in yeast, this implies that the Golgi apparatus can function with a single syntaxin, Sed5p. ..
  36. Zou S, Liu Y, Zhang X, Chen Y, Ye M, Zhu X, et al. Modular TRAPP complexes regulate intracellular protein trafficking through multiple Ypt/Rab GTPases in Saccharomyces cerevisiae. Genetics. 2012;191:451-60 pubmed publisher
    ..Here, we use GFP-Snc1 as a tool to study transport in Ypt and TRAPP mutant cells...
  37. Siniossoglou S, Pelham H. An effector of Ypt6p binds the SNARE Tlg1p and mediates selective fusion of vesicles with late Golgi membranes. EMBO J. 2001;20:5991-8 pubmed
    ..We suggest that the VFT complex links vesicles containing Tlg1p to their target, which is defined by the local activation of Ypt6p. ..
  38. Williams D, Novick P. Analysis of SEC9 suppression reveals a relationship of SNARE function to cell physiology. PLoS ONE. 2009;4:e5449 pubmed publisher
    ..Second, Sro7p acts to promote SNARE complex formation. Finally, Sec9p function and SNARE complex formation are tightly coupled to the physiological state of the cell. ..
  39. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, et al. The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol. 2013;202:509-26 pubmed publisher
    ..We propose that the exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins. This could help to confer both specificity and directionality to vesicular traffic. ..