SMD1

Summary

Gene Symbol: SMD1
Description: mRNA splicing protein SMD1
Alias: SPP92, mRNA splicing protein SMD1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Neubauer G, Gottschalk A, Fabrizio P, Seraphin B, Luhrmann R, Mann M. Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry. Proc Natl Acad Sci U S A. 1997;94:385-90 pubmed
    ..The technique should be applicable to any protein complex that can be biochemically purified from an organism whose genome is known. ..
  2. Gottschalk A, Tang J, Puig O, Salgado J, Neubauer G, Colot H, et al. A comprehensive biochemical and genetic analysis of the yeast U1 snRNP reveals five novel proteins. RNA. 1998;4:374-93 pubmed
    ..Finally, we show that Nam8p/Mud15p contributes to the stability of U1 snRNP. ..
  3. Gottschalk A, Kastner B, Luhrmann R, Fabrizio P. The yeast U5 snRNP coisolated with the U1 snRNP has an unexpected protein composition and includes the splicing factor Aar2p. RNA. 2001;7:1554-65 pubmed
    ..Thus, the basic structural scaffold of the Aar2-U5 snRNP seems to be essentially determined by Prp8p, Snu114p, and the Sm proteins. ..
  4. Schwer B, Roth A, Shuman S. Will the circle be unbroken: specific mutations in the yeast Sm protein ring expose a requirement for assembly factor Brr1, a homolog of Gemin2. RNA. 2017;23:420-430 pubmed publisher
    ..mutagenesis in Saccharomyces cerevisiae, we gained new insights into structure-function relationships of the SmD1 and SmD2 subunits...
  5. Rymond B. Convergent transcripts of the yeast PRP38-SMD1 locus encode two essential splicing factors, including the D1 core polypeptide of small nuclear ribonucleoprotein particles. Proc Natl Acad Sci U S A. 1993;90:848-52 pubmed
    ..This report describes the identification of a gene of related function, SMD1, located immediately 3' to PRP38...
  6. Zhang D, Rosbash M. Identification of eight proteins that cross-link to pre-mRNA in the yeast commitment complex. Genes Dev. 1999;13:581-92 pubmed
  7. Fortes P, Bilbao Cortes D, Fornerod M, Rigaut G, Raymond W, Seraphin B, et al. Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site recognition. Genes Dev. 1999;13:2425-38 pubmed
    ..These data suggest that the loss of Luc7p disrupts U1 snRNP-CBC interaction, and that this interaction contributes to normal 5' splice site recognition. ..
  8. Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Seraphin B. A generic protein purification method for protein complex characterization and proteome exploration. Nat Biotechnol. 1999;17:1030-2 pubmed
    ..Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The TAP method has been tested in yeast but should be applicable to other cells or organisms. ..
  9. Boon K, Norman C, Grainger R, Newman A, Beggs J. Prp8p dissection reveals domain structure and protein interaction sites. RNA. 2006;12:198-205 pubmed
    ..Thus, the U5 snRNP protein Snu114p associates with Prp8p in the region 437-770, whereas fragmenting Prp8p at residue 2173 destabilizes its association with Aar2p. ..

More Information

Publications13

  1. Chang K, Chen H, Cheng S. Ntc90 is required for recruiting first step factor Yju2 but not for spliceosome activation. RNA. 2009;15:1729-39 pubmed publisher
    ..Our results demonstrate a novel role of the NTC in recruiting splicing factors to the spliceosome after its activation. ..
  2. Yeh C, Chang S, Chen J, Wang H, Chou Y, Wang C, et al. The conserved AU dinucleotide at the 5' end of nascent U1 snRNA is optimized for the interaction with nuclear cap-binding-complex. Nucleic Acids Res. 2017;45:9679-9693 pubmed publisher
    ..Our data also provide a structural interpretation as to why the AU dinucleotide is conserved during evolution. ..
  3. Xue D, Rubinson D, Pannone B, Yoo C, Wolin S. U snRNP assembly in yeast involves the La protein. EMBO J. 2000;19:1650-60 pubmed
    ..At the permissive temperature, smd1-1 cells contain higher levels of stable U1 and U5 snRNPs when Lhp1p is present...
  4. Häcker I, Sander B, Golas M, Wolf E, Karagöz E, Kastner B, et al. Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy. Nat Struct Mol Biol. 2008;15:1206-12 pubmed publisher
    ..The head and arm adopt variable relative positions. This molecular organization and dynamics suggest possible scenarios for structural events during catalytic activation. ..