SMC5

Summary

Gene Symbol: SMC5
Description: DNA repair ATPase SMC5
Alias: DNA repair ATPase SMC5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. De Piccoli G, Cortes Ledesma F, Ira G, Torres Rosell J, Uhle S, Farmer S, et al. Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination. Nat Cell Biol. 2006;8:1032-4 pubmed
    ..Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister ..
  2. Chen Y, Choi K, Szakal B, Arenz J, Duan X, Ye H, et al. Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair. Proc Natl Acad Sci U S A. 2009;106:21252-7 pubmed publisher
    The evolutionarily conserved Smc5/6 complex is implicated in recombinational repair, but its function in this process has been elusive. Here we report that the budding yeast Smc5/6 complex directly binds to the DNA helicase Mph1...
  3. Duan X, Yang Y, Chen Y, Arenz J, Rangi G, Zhao X, et al. Architecture of the Smc5/6 Complex of Saccharomyces cerevisiae Reveals a Unique Interaction between the Nse5-6 Subcomplex and the Hinge Regions of Smc5 and Smc6. J Biol Chem. 2009;284:8507-15 pubmed publisher
    ..SMC) proteins forms the core structures of three multisubunit complexes as follows: cohesin, condensin, and the Smc5/6 complex...
  4. Takahashi Y, Dulev S, Liu X, Hiller N, Zhao X, Strunnikov A. Cooperation of sumoylated chromosomal proteins in rDNA maintenance. PLoS Genet. 2008;4:e1000215 pubmed publisher
    ..In addition, binding of cohesin and condensin to rDNA is altered in the mms21-CH E3-deficient mutant. ..
  5. Lindroos H, Ström L, Itoh T, Katou Y, Shirahige K, Sjögren C. Chromosomal association of the Smc5/6 complex reveals that it functions in differently regulated pathways. Mol Cell. 2006;22:755-67 pubmed
    ..The chromosomal localization of the budding yeast Smc5/6 complex determined here reveals that the complex works specifically on the duplicated genome in differently ..
  6. Zhao X, Blobel G. A SUMO ligase is part of a nuclear multiprotein complex that affects DNA repair and chromosomal organization. Proc Natl Acad Sci U S A. 2005;102:4777-82 pubmed
    ..of small ubiquitin-like modifier (SUMO) ligases, two structural-maintenance-of-chromosome (Smc) proteins, Smc5 and Smc6, and a protein that contains an ubiquitin ligase signature domain...
  7. Leung G, Lee L, Schmidt T, Shirahige K, Kobor M. Rtt107 is required for recruitment of the SMC5/6 complex to DNA double strand breaks. J Biol Chem. 2011;286:26250-7 pubmed publisher
    ..These include the evolutionarily conserved SMC5/6 complex, which is involved in numerous chromosome maintenance activities, such as DNA repair, chromosome ..
  8. Cost G, Cozzarelli N. Smc5p promotes faithful chromosome transmission and DNA repair in Saccharomyces cerevisiae. Genetics. 2006;172:2185-200 pubmed
    ..To better understand them, we created two S. cerevisiae strains bearing temperature-sensitive alleles of SMC5. When shifted to the restrictive temperature, both mutants lose viability gradually, concomitant with the ..
  9. Torres Rosell J, Machin F, Farmer S, Jarmuz A, Eydmann T, Dalgaard J, et al. SMC5 and SMC6 genes are required for the segregation of repetitive chromosome regions. Nat Cell Biol. 2005;7:412-9 pubmed
    Structure chromosome (SMC) proteins organize the core of cohesin, condensin and Smc5-Smc6 complexes. The Smc5-Smc6 complex is required for DNA repair, as well as having another essential but enigmatic function...

More Information

Publications45

  1. Roy M, Siddiqui N, D Amours D. Dynamic and selective DNA-binding activity of Smc5, a core component of the Smc5-Smc6 complex. Cell Cycle. 2011;10:690-700 pubmed
    ..In particular, the conserved Smc5-6 complex is required for efficient DNA repair, checkpoint signaling, and DNA replication in all eukaryotes...
  2. Copsey A, Tang S, Jordan P, Blitzblau H, Newcombe S, Chan A, et al. Smc5/6 coordinates formation and resolution of joint molecules with chromosome morphology to ensure meiotic divisions. PLoS Genet. 2013;9:e1004071 pubmed publisher
    ..meiotic functions of the cohesin and condensin complexes have been delineated, the role of the third SMC complex, Smc5/6, remains enigmatic...
  3. Bustard D, Menolfi D, Jeppsson K, Ball L, Dewey S, Shirahige K, et al. During replication stress, non-SMC element 5 (NSE5) is required for Smc5/6 protein complex functionality at stalled forks. J Biol Chem. 2012;287:11374-83 pubmed publisher
    The Smc5/6 complex belongs to the SMC (structural maintenance of chromosomes) family, which also includes cohesin and condensin. In Saccharomyces cerevisiae, the Smc5/6 complex contains six essential non-Smc elements, Nse1-6...
  4. Sollier J, Driscoll R, Castellucci F, Foiani M, Jackson S, Branzei D. The Saccharomyces cerevisiae Esc2 and Smc5-6 proteins promote sister chromatid junction-mediated intra-S repair. Mol Biol Cell. 2009;20:1671-82 pubmed publisher
    ..We show that mutations in Smc5-6 and Esc2 also lead to an accumulation of recombinogenic structures at damaged replication forks...
  5. Duan X, Sarangi P, Liu X, Rangi G, Zhao X, Ye H. Structural and functional insights into the roles of the Mms21 subunit of the Smc5/6 complex. Mol Cell. 2009;35:657-68 pubmed publisher
    The Smc5/6 complex is an evolutionarily conserved chromosomal ATPase required for cell growth and DNA repair. Its Mms21 subunit supports both functions by docking to the arm region of Smc5 and providing SUMO ligase activity...
  6. Wohlschlegel J, Johnson E, Reed S, Yates J. Global analysis of protein sumoylation in Saccharomyces cerevisiae. J Biol Chem. 2004;279:45662-8 pubmed
    ..Additionally, our global analysis has revealed a number of interesting biological patterns in the list of SUMO targets including a clustering of sumoylation targets within macromolecular complexes. ..
  7. Xaver M, Huang L, Chen D, Klein F. Smc5/6-Mms21 prevents and eliminates inappropriate recombination intermediates in meiosis. PLoS Genet. 2013;9:e1004067 pubmed publisher
    ..Here, we report the requirement of Smc5/6-Mms21 for antagonizing rogue JMs via two mechanisms; destabilizing early intermediates and resolving JMs...
  8. Lilienthal I, Kanno T, Sjögren C. Inhibition of the Smc5/6 complex during meiosis perturbs joint molecule formation and resolution without significantly changing crossover or non-crossover levels. PLoS Genet. 2013;9:e1003898 pubmed publisher
    ..Meiosis-specific smc5/6 mutants experience a block in DNA segregation without hindering meiotic progression...
  9. Chavez A, Agrawal V, Johnson F. Homologous recombination-dependent rescue of deficiency in the structural maintenance of chromosomes (Smc) 5/6 complex. J Biol Chem. 2011;286:5119-25 pubmed publisher
    The essential and evolutionarily conserved Smc5-Smc6 complex (Smc5/6) is critical for the maintenance of genome stability...
  10. Chavez A, George V, Agrawal V, Johnson F. Sumoylation and the structural maintenance of chromosomes (Smc) 5/6 complex slow senescence through recombination intermediate resolution. J Biol Chem. 2010;285:11922-30 pubmed publisher
    ..One target of Mms21-dependent sumoylation is the cohesin- and condensin-related Smc5-Smc6 complex (Smc5/6)...
  11. Cremona C, Sarangi P, Yang Y, Hang L, Rahman S, Zhao X. Extensive DNA damage-induced sumoylation contributes to replication and repair and acts in addition to the mec1 checkpoint. Mol Cell. 2012;45:422-32 pubmed publisher
    ..We also show that DNA damage-induced sumoylation does not require Mec1 checkpoint signaling, and the presence of both enables optimal DNA damage resistance. ..
  12. Choi K, Szakal B, Chen Y, Branzei D, Zhao X. The Smc5/6 complex and Esc2 influence multiple replication-associated recombination processes in Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2306-14 pubmed publisher
    ..However, their functions become detrimental when cells lack the Smc5/6 complex or Esc2...
  13. Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D. Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance. Mol Cell. 2015;60:835-46 pubmed publisher
    The essential functions of the conserved Smc5/6 complex remain elusive...
  14. Dummer A, Su Z, Cherney R, Choi K, DENU J, Zhao X, et al. Binding of the Fkh1 Forkhead Associated Domain to a Phosphopeptide within the Mph1 DNA Helicase Regulates Mating-Type Switching in Budding Yeast. PLoS Genet. 2016;12:e1006094 pubmed publisher
  15. Kim D, Harris B, Wang F, Seidel C, McCroskey S, Gerton J. Mms21 SUMO Ligase Activity Promotes Nucleolar Function in Saccharomyces cerevisiae. Genetics. 2016;204:645-658 pubmed
    The budding yeast E3 SUMO ligase Mms21, also known as Nse2, is a component of the Smc5/6 complex, which regulates sister chromatid cohesion, DNA replication, and repair...
  16. Duan X, Holmes W, Ye H. Interaction mapping between Saccharomyces cerevisiae Smc5 and SUMO E3 ligase Mms21. Biochemistry. 2011;50:10182-8 pubmed publisher
    The multisubunit Smc5-Smc6 holocomplex (Smc5/6) plays a critical role in chromosome stability maintenance, DNA replication, homologous recombination, and double-stranded DNA damage repair...
  17. Tapia Alveal C, Lin S, O Connell M. Functional interplay between cohesin and Smc5/6 complexes. Chromosoma. 2014;123:437-45 pubmed publisher
    ..complexes collectively known as the structural maintenance of chromosome (SMC) complexes: cohesin, condensin, and Smc5/6...
  18. Xue X, Choi K, Bonner J, Chiba T, Kwon Y, Xu Y, et al. Restriction of replication fork regression activities by a conserved SMC complex. Mol Cell. 2014;56:436-45 pubmed publisher
    ..We show that the structural maintenance of chromosome complex Smc5-Smc6 restrains the replication fork regression activity of Mph1 helicase, but not its D loop disruptive activity...
  19. Bermúdez López M, Pociño Merino I, Sánchez H, Bueno A, Guasch C, Almedawar S, et al. ATPase-dependent control of the Mms21 SUMO ligase during DNA repair. PLoS Biol. 2015;13:e1002089 pubmed publisher
    ..The Mms21 SUMO ligase docks to the arm region of the Smc5 protein in the Smc5/6 complex; together, they cooperate during recombinational DNA repair...
  20. Moradi Fard S, Sarthi J, Tittel Elmer M, Lalonde M, Cusanelli E, Chartrand P, et al. Smc5/6 Is a Telomere-Associated Complex that Regulates Sir4 Binding and TPE. PLoS Genet. 2016;12:e1006268 pubmed publisher
    SMC proteins constitute the core members of the Smc5/6, cohesin and condensin complexes...
  21. Noël J, Wellinger R. Abrupt telomere losses and reduced end-resection can explain accelerated senescence of Smc5/6 mutants lacking telomerase. DNA Repair (Amst). 2011;10:271-82 pubmed publisher
    ..Recently, the Smc5/6 complex has been reported to be important for telomere maintenance in yeast and also in cancerous human ALT cells,..
  22. Ohouo P, Smolka M. A touching moment for Smc5/6: from ssDNA binding to repair. Cell Cycle. 2011;10:1190-1 pubmed
  23. Ohouo P, Bastos de Oliveira F, Almeida B, Smolka M. DNA damage signaling recruits the Rtt107-Slx4 scaffolds via Dpb11 to mediate replication stress response. Mol Cell. 2010;39:300-6 pubmed publisher
    ..Our results unveil how Mec1 regulates the Slx4 and Rtt107 scaffolds and establish a mechanistic link between DNA damage signaling and fork repair. ..
  24. Fujioka Y, Kimata Y, Nomaguchi K, Watanabe K, Kohno K. Identification of a novel non-structural maintenance of chromosomes (SMC) component of the SMC5-SMC6 complex involved in DNA repair. J Biol Chem. 2002;277:21585-91 pubmed
    ..and SMC1-SMC3 complexes are also known as condensin and cohesin, respectively, but the recently identified SMC5 and SMC6 complex is less well characterized...
  25. Laflamme G, Tremblay Boudreault T, Roy M, Andersen P, Bonneil E, Atchia K, et al. Structural maintenance of chromosome (SMC) proteins link microtubule stability to genome integrity. J Biol Chem. 2014;289:27418-31 pubmed publisher
    ..We mapped the microtubule-binding region of Smc5 and generated a mutant with impaired microtubule binding activity...
  26. Duan X, Ye H. Purification, crystallization and preliminary X-ray crystallographic studies of the complex between Smc5 and the SUMO E3 ligase Mms21. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:849-52 pubmed publisher
    b>Smc5/6, a protein complex that belongs to the structural maintenance of chromosome (SMC) family, plays a key role in DNA replication, sister chromatid recombination and DNA damage repair...
  27. Santa Maria S, Gangavarapu V, Johnson R, Prakash L, Prakash S. Requirement of Nse1, a subunit of the Smc5-Smc6 complex, for Rad52-dependent postreplication repair of UV-damaged DNA in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:8409-18 pubmed
    ..In this study, we present evidence for the requirement of Nse1, a subunit of the Smc5-Smc6 complex, in Rad52-dependent PRR, and our genetic analyses suggest a role for the Nse1 and Mms21 E3 ligase ..
  28. Xue X, Choi K, Bonner J, Szakal B, Chen Y, Papusha A, et al. Selective modulation of the functions of a conserved DNA motor by a histone fold complex. Genes Dev. 2015;29:1000-5 pubmed publisher
    ..Mechanistically, scMHF relieves the inhibition imposed by the structural maintenance of chromosome protein Smc5 on Mph1 activities relevant to replication-associated repair through binding to Mph1 but not DNA...
  29. Kanno T, Berta D, Sjögren C. The Smc5/6 Complex Is an ATP-Dependent Intermolecular DNA Linker. Cell Rep. 2015;12:1471-82 pubmed publisher
    The structural maintenance of chromosome (SMC) protein complexes cohesin and condensin and the Smc5/6 complex (Smc5/6) are crucial for chromosome dynamics and stability...
  30. Lafuente Barquero J, Luke Glaser S, Graf M, Silva S, Gómez González B, Lockhart A, et al. The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. PLoS Genet. 2017;13:e1007136 pubmed publisher
    ..Mph1, however is a double-edged sword, whose action at hybrids must be regulated by the Smc5/6 complex...
  31. Jeppsson K, Carlborg K, Nakato R, Berta D, Lilienthal I, Kanno T, et al. The chromosomal association of the Smc5/6 complex depends on cohesion and predicts the level of sister chromatid entanglement. PLoS Genet. 2014;10:e1004680 pubmed publisher
    ..The cohesin-related Smc5/6 complex (Smc5/6) instead accumulates on chromosomes after Top2 inactivation, known to lead to a buildup of ..
  32. Torres Rosell J, Sunjevaric I, De Piccoli G, Sacher M, Eckert Boulet N, Reid R, et al. The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus. Nat Cell Biol. 2007;9:923-31 pubmed
    ..The nucleolar exclusion of Rad52 recombination foci entails Mre11 and Smc5-Smc6 complexes and depends on Rad52 SUMO (small ubiquitin-related modifier) modification...
  33. Torres Rosell J, Machin F, Aragon L. Smc5-Smc6 complex preserves nucleolar integrity in S. cerevisiae. Cell Cycle. 2005;4:868-72 pubmed
    ..A third complex containing Smc proteins at its core, the Smc5-Smc6 complex, previously known to be involved in DNA repair has recently been shown to contribute to chromosome ..
  34. Watanabe Y. The importance of being Smc5/6. Nat Cell Biol. 2005;7:329-31 pubmed
  35. Hu B, Liao C, Millson S, Mollapour M, Prodromou C, Pearl L, et al. Qri2/Nse4, a component of the essential Smc5/6 DNA repair complex. Mol Microbiol. 2005;55:1735-50 pubmed
    We demonstrate a role for Qri2 in the essential DNA repair function of the Smc5/6 complex in Saccharomyces cerevisiae. We generated temperature-sensitive (ts) mutants in QRI2 and characterized their properties...
  36. Rai R, Laloraya S. Genetic evidence for functional interaction of Smc5/6 complex and Top1 with spatial frequency of replication origins required for maintenance of chromosome stability. Curr Genet. 2017;63:765-776 pubmed publisher
    ..The Smc5/6 complex is thought to play an important role in replication by its involvement in the restart of collapsed ..