Gene Symbol: SLA2
Description: Sla2p
Alias: END4, MOP2, Sla2p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Na S, Hincapie M, McCusker J, Haber J. MOP2 (SLA2) affects the abundance of the plasma membrane H(+)-ATPase of Saccharomyces cerevisiae. J Biol Chem. 1995;270:6815-23 pubmed
    ..gene encodes a 108-kDa protein that has previously been identified both as a gene affecting the yeast cytoskeleton (SLA2) (Holtzman, D.A., Yang, S., and Drubin, D. G. (1993) J. Cell Biol...
  2. Yoshiuchi S, Yamamoto T, Sakane H, Kadota J, Mochida J, Asaka M, et al. Identification of novel mutations in ACT1 and SLA2 that suppress the actin-cable-overproducing phenotype caused by overexpression of a dominant active form of Bni1p in Saccharomyces cerevisiae. Genetics. 2006;173:527-39 pubmed
    ..Two recessive mutations, act1-301 in the actin gene and sla2-82 in a gene involved in cortical actin patch assembly, were identified...
  3. Holtzman D, Yang S, Drubin D. Synthetic-lethal interactions identify two novel genes, SLA1 and SLA2, that control membrane cytoskeleton assembly in Saccharomyces cerevisiae. J Cell Biol. 1993;122:635-44 pubmed
    ..The COOH terminus of Sla2p contains a 200 amino acid region with homology to the COOH terminus of talin, a membrane cytoskeletal protein ..
  4. Raths S, Rohrer J, Crausaz F, Riezman H. end3 and end4: two mutants defective in receptor-mediated and fluid-phase endocytosis in Saccharomyces cerevisiae. J Cell Biol. 1993;120:55-65 pubmed
    ..The endocytic defect in end4 cells appears immediately upon shift to restrictive temperature and is reversible at permissive temperature if new ..
  5. Wesp A, Hicke L, Palecek J, Lombardi R, Aust T, Munn A, et al. End4p/Sla2p interacts with actin-associated proteins for endocytosis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:2291-306 pubmed
    b>end4-1 was isolated as a temperature-sensitive endocytosis mutant. We cloned and sequenced END4 and found that it is identical to SLA2/MOP2...
  6. Pruyne D, Bretscher A. Polarization of cell growth in yeast. J Cell Sci. 2000;113 ( Pt 4):571-85 pubmed
    ..The dynamic arrangement of targeting and recycling provides flexibility for the precise control of morphogenesis. ..
  7. Mochida J, Yamamoto T, Fujimura Kamada K, Tanaka K. The novel adaptor protein, Mti1p, and Vrp1p, a homolog of Wiskott-Aldrich syndrome protein-interacting protein (WIP), may antagonistically regulate type I myosins in Saccharomyces cerevisiae. Genetics. 2002;160:923-34 pubmed
    ..A null mutation of MTI1 exhibited synthetic lethal phenotypes with mutations in SAC6 and SLA2, which encode actin-bundling and cortical actin-binding proteins, respectively...
  8. Valdivia R, Baggott D, Chuang J, Schekman R. The yeast clathrin adaptor protein complex 1 is required for the efficient retention of a subset of late Golgi membrane proteins. Dev Cell. 2002;2:283-94 pubmed
    ..Similarly, in AP-1 deficient cells, the resident TGN/early endosome syntaxin, Tlg1p, is missorted. We propose that clathrin and AP-1 act to recycle Chs3p and Tlg1p from the early endosome to the TGN. ..
  9. Toshima J, Horikomi C, Okada A, Hatori M, Nagano M, Masuda A, et al. Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis. J Cell Sci. 2016;129:367-79 pubmed publisher
    ..These results demonstrate the in vivo roles of Srv2p in the regulation of the actin cytoskeleton during clathrin-mediated endocytosis. ..

More Information


  1. Baggett J, D Aquino K, Wendland B. The Sla2p talin domain plays a role in endocytosis in Saccharomyces cerevisiae. Genetics. 2003;165:1661-74 pubmed
    ..temperature, that SLA2 exhibits genetic interactions with both ENT1 and ENT2, and that the clathrin adaptors and Sla2p together regulate the actin cytoskeleton and revealed conditions under which Yap1801p and Yap1802p contribute to ..
  2. Bidlingmaier S, Snyder M. Large-scale identification of genes important for apical growth in Saccharomyces cerevisiae by directed allele replacement technology (DART) screening. Funct Integr Genomics. 2002;1:345-56 pubmed
    ..We identified 29 insertion alleles, containing mutations in 17 different genes ( SMY1, SPA2, PAN1, SLA1, SLA2, CBK1, SEC22, FAB1, VPS36, VID22, RAS2, ECM33, OPI3, API1/YDR372c, API2/YDR525w, API3/YKR020w, and API4/YNL051w), ..
  3. Palecek J, Hasek J, Ruis H. Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochem Biophys Res Commun. 2001;282:1244-50 pubmed
    ..They overlapped in the region of amino acids 318-550. Subsequent deletion analysis of Sla2p showed that amino acids 318-373 were essential for the two-hybrid protein-protein interaction...
  4. Conibear E, Stevens T. Vps52p, Vps53p, and Vps54p form a novel multisubunit complex required for protein sorting at the yeast late Golgi. Mol Biol Cell. 2000;11:305-23 pubmed
    ..The Vps52/53/54 complex joins a growing list of distinct multisubunit complexes that regulate membrane-trafficking events. ..
  5. Gale C, Leonard M, Finley K, Christensen L, McClellan M, Abbey D, et al. SLA2 mutations cause SWE1-mediated cell cycle phenotypes in Candida albicans and Saccharomyces cerevisiae. Microbiology. 2009;155:3847-59 pubmed publisher
    The early endocytic patch protein Sla2 is important for morphogenesis and growth rates in Saccharomyces cerevisiae and Candida albicans, but the mechanism that connects these processes is not clear...
  6. McCourt P, Morgan J, Nickels J. Stress-induced ceramide-activated protein phosphatase can compensate for loss of amphiphysin-like activity in Saccharomyces cerevisiae and functions to reinitiate endocytosis. J Biol Chem. 2009;284:11930-41 pubmed publisher
    ..Finally, genetic analyses reveal a synthetic lethal interaction between loss of CDC55 and gene deletions lethal with rvs161, all of which function in endocytosis. ..
  7. Haynes J, Garcia B, Stollar E, Rath A, Andrews B, Davidson A. The biologically relevant targets and binding affinity requirements for the function of the yeast actin-binding protein 1 Src-homology 3 domain vary with genetic context. Genetics. 2007;176:193-208 pubmed
    ..genetic backgrounds (prk1 Delta and sla1 Delta) tolerated large affinity reductions while others (sac6 Delta and sla2 Delta) were much more sensitive to these reductions...
  8. Yang S, Cope M, Drubin D. Sla2p is associated with the yeast cortical actin cytoskeleton via redundant localization signals. Mol Biol Cell. 1999;10:2265-83 pubmed
    b>Sla2p, also known as End4p and Mop2p, is the founding member of a widely conserved family of actin-binding proteins, a distinguishing feature of which is a C-terminal region homologous to the C terminus of talin...
  9. Nothwehr S, Conibear E, Stevens T. Golgi and vacuolar membrane proteins reach the vacuole in vps1 mutant yeast cells via the plasma membrane. J Cell Biol. 1995;129:35-46 pubmed
    ..of these proteins in vps1 delta cells that also contained temperature sensitive mutations in either the SEC4 or END4 genes, which are required for a late step in secretion and the internalization step of endocytosis, respectively...
  10. Blader I, Cope M, Jackson T, Profit A, Greenwood A, Drubin D, et al. GCS1, an Arf guanosine triphosphatase-activating protein in Saccharomyces cerevisiae, is required for normal actin cytoskeletal organization in vivo and stimulates actin polymerization in vitro. Mol Biol Cell. 1999;10:581-96 pubmed
    ..Synthetic lethality was observed between null alleles of GCS1 and SLA2, the gene encoding a protein involved in stabilization of the actin cytoskeleton...
  11. Yamamoto T, Mochida J, Kadota J, Takeda M, Bi E, Tanaka K. Initial polarized bud growth by endocytic recycling in the absence of actin cable-dependent vesicle transport in yeast. Mol Biol Cell. 2010;21:1237-52 pubmed publisher
  12. Fiorani P, Reid R, Schepis A, Jacquiau H, Guo H, Thimmaiah P, et al. The deubiquitinating enzyme Doa4p protects cells from DNA topoisomerase I poisons. J Biol Chem. 2004;279:21271-81 pubmed
    ..Mutant alleles of three genes, DOA4, SLA1 and SLA2, were recovered...
  13. Fernández Golbano I, Idrissi F, Giblin J, Grosshans B, Robles V, Grötsch H, et al. Crosstalk between PI(4,5)P₂and CK2 modulates actin polymerization during endocytic uptake. Dev Cell. 2014;30:746-58 pubmed publisher
    ..Our data demonstrate a holoenzyme-independent function of CK2 in endocytic budding and establish a robust genetic, functional, and molecular link between PI(4,5)Pâ‚‚and CK2, two masters of intracellular signaling. ..
  14. Zhang F, Gaur N, Hasek J, Kim S, Qiu H, Swanson M, et al. Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4. Mol Cell Biol. 2008;28:6796-818 pubmed publisher
  15. Newpher T, Lemmon S. Clathrin is important for normal actin dynamics and progression of Sla2p-containing patches during endocytosis in yeast. Traffic. 2006;7:574-88 pubmed
    ..Here, we investigate the dynamics of clathrin relative to the early endocytic patch protein Sla2p. We find that clathrin is recruited to the cortex prior to Sla2p...
  16. Marx U, Polakowski T, Pomorski T, Lang C, Nelson H, Nelson N, et al. Rapid transbilayer movement of fluorescent phospholipid analogues in the plasma membrane of endocytosis-deficient yeast cells does not require the Drs2 protein. Eur J Biochem. 1999;263:254-63 pubmed
    Evidence is presented that endocytosis-deficient Saccharomyces cerevisiae end4 yeast cells rapidly internalize the fluorescent phospholipid analogues 1-palmitoyl-2-{6-[7-nitro-2,1, 3-benzoxadiazol-4-yl(NBD)amino] caproyl}..
  17. Li L, Vulpe C, Kaplan J. Functional studies of hephaestin in yeast: evidence for multicopper oxidase activity in the endocytic pathway. Biochem J. 2003;375:793-8 pubmed
  18. Munn A, Riezman H. Endocytosis is required for the growth of vacuolar H(+)-ATPase-defective yeast: identification of six new END genes. J Cell Biol. 1994;127:373-86 pubmed
    ..The mutations that result in a defect in alpha-factor uptake are not allelic to any of the genes previously shown to encode endocytic functions. ..
  19. McCann R, Craig S. The I/LWEQ module: a conserved sequence that signifies F-actin binding in functionally diverse proteins from yeast to mammals. Proc Natl Acad Sci U S A. 1997;94:5679-84 pubmed
    ..The C-terminal 197 amino acids of vertebrate talin are 45% similar to the C-terminal residues of Sla2, a yeast protein implicated in polarized assembly of the yeast actin cytoskeleton...
  20. Toshima J, Toshima J, Duncan M, Cope M, Sun Y, Martin A, et al. Negative regulation of yeast Eps15-like Arp2/3 complex activator, Pan1p, by the Hip1R-related protein, Sla2p, during endocytosis. Mol Biol Cell. 2007;18:658-68 pubmed
    ..affinity purification-tagged Pan1p, we identified End3p as a stoichiometric component of the Pan1p complex, and Sla2p, a yeast Hip1R-related protein, as a novel binding partner of Pan1p...
  21. Wendland B, McCaffery J, Xiao Q, Emr S. A novel fluorescence-activated cell sorter-based screen for yeast endocytosis mutants identifies a yeast homologue of mammalian eps15. J Cell Biol. 1996;135:1485-500 pubmed
    ..In addition, double mutants with either dim1-delta or dim2-1 and the endocytosis mutants end4-1 or act1-1 displayed synthetic growth defects, indicating that the DIM gene products function in a common or ..
  22. Yang S, Ayscough K, Drubin D. A role for the actin cytoskeleton of Saccharomyces cerevisiae in bipolar bud-site selection. J Cell Biol. 1997;136:111-23 pubmed
    ..A defective bipolar budding pattern did not preclude an sla2-6 mutant from undergoing pseudohyphal growth, highlighting the central role of daughter cell bud-site selection ..
  23. Smaczynska de Rooij I, Costa R, Ayscough K. Yeast Arf3p modulates plasma membrane PtdIns(4,5)P2 levels to facilitate endocytosis. Traffic. 2008;9:559-73 pubmed publisher
    ..Our data provide evidence for a mechanism in yeast to positively regulate plasma membrane production of PtdIns(4,5)P2 levels and that these changes impact on endocytosis. ..
  24. Gourlay C, Dewar H, Warren D, Costa R, Satish N, Ayscough K. An interaction between Sla1p and Sla2p plays a role in regulating actin dynamics and endocytosis in budding yeast. J Cell Sci. 2003;116:2551-64 pubmed
    ..Here we show the importance of two yeast proteins in this process. We demonstrate that Sla1p and Sla2p interact in vitro and in vivo and that this interaction is mediated by the central domain of Sla2p, which includes ..
  25. Moreau V, Galan J, Devilliers G, Haguenauer Tsapis R, Winsor B. The yeast actin-related protein Arp2p is required for the internalization step of endocytosis. Mol Biol Cell. 1997;8:1361-75 pubmed
    ..Finally, no evidence for a major defect in secretion was apparent; invertase secretion and delivery of uracil permease to the plasma membrane were unaffected in arp2-1 cells. ..
  26. Newpher T, Idrissi F, Geli M, Lemmon S. Novel function of clathrin light chain in promoting endocytic vesicle formation. Mol Biol Cell. 2006;17:4343-52 pubmed
    ..HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding yeast homologue, Sla2p. Here, using in vivo analysis in yeast, we provide novel insight into the role of this interaction...
  27. McCann R, Craig S. Functional genomic analysis reveals the utility of the I/LWEQ module as a predictor of protein:actin interaction. Biochem Biophys Res Commun. 1999;266:135-40 pubmed
    ..we have identified as an actin-binding motif in the metazoan focal adhesion protein talin and the yeast protein Sla2p. Both of these proteins are associated with the actin cytoskeleton in cells...
  28. Chin C, Bennett A, Ma W, Hall M, Yeong F. Dependence of Chs2 ER export on dephosphorylation by cytoplasmic Cdc14 ensures that septum formation follows mitosis. Mol Biol Cell. 2012;23:45-58 pubmed publisher
    ..Thus, by requiring declining Cdk1 activity and Cdc14 nuclear release for Chs2 ER export, cells ensure that septum formation is contingent upon chromosome separation and exit from mitosis. ..
  29. Li L, Chen O, McVey Ward D, Kaplan J. CCC1 is a transporter that mediates vacuolar iron storage in yeast. J Biol Chem. 2001;276:29515-9 pubmed
    ..We demonstrated that endocytosis-defective yeast (Delta end4) can store iron in the vacuole, indicating a transfer of iron from the cytosol to the vacuole...
  30. Neuber A, Franke J, Wittstruck A, Schlenstedt G, Sommer T, Stade K. Nuclear export receptor Xpo1/Crm1 is physically and functionally linked to the spindle pole body in budding yeast. Mol Cell Biol. 2008;28:5348-58 pubmed publisher
    ..In addition, we find a subpopulation of Xpo1 localized at the SPB. Based on these data, we propose a functional link between Xpo1 and the SPB and discuss a role for this exportin in spindle biogenesis in budding yeast. ..
  31. Henry K, D hondt K, Chang J, Newpher T, Huang K, Hudson R, et al. Scd5p and clathrin function are important for cortical actin organization, endocytosis, and localization of sla2p in yeast. Mol Biol Cell. 2002;13:2607-25 pubmed
    ..Both Scd5p and yeast clathrin physically associate with Sla2p, a homologue of the mammalian huntingtin interacting protein HIP1 and the related HIP1R...
  32. Ganusova E, Ozolins L, Bhagat S, Newnam G, Wegrzyn R, Sherman M, et al. Modulation of prion formation, aggregation, and toxicity by the actin cytoskeleton in yeast. Mol Cell Biol. 2006;26:617-29 pubmed
    ..Deletion of the gene coding for the actin assembly protein Sla2 is lethal in cells containing the prion isoforms of both Sup35 and Rnq1 proteins simultaneously...
  33. Reyes A, Sanz M, Duran A, Roncero C. Chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane. J Cell Sci. 2007;120:1998-2009 pubmed
    ..Chs4p thus promotes Chs3p translocation into the plasma membrane in a stable and active form. Proper CSIII turnover is maintained through the endocytic internalization of Chs3p. ..
  34. Newpher T, Smith R, Lemmon V, Lemmon S. In vivo dynamics of clathrin and its adaptor-dependent recruitment to the actin-based endocytic machinery in yeast. Dev Cell. 2005;9:87-98 pubmed
    ..In contrast, Sla2p, End3p, Pan1p, and a dynamic actin cytoskeleton are not required for clathrin assembly or exchange but are ..
  35. Kaminska J, Gajewska B, Hopper A, Zoładek T. Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:6946-8 pubmed
    ..The defects in endocytosis, actin cytoskeleton, and morphology of arp2 are also suppressed. (ii) Rsp5p and Sla2p colocalize in abnormal F-actin-containing clumps in arp2 and pan1 mutants...
  36. Bagriantsev S, Gracheva E, Richmond J, Liebman S. Variant-specific [PSI+] infection is transmitted by Sup35 polymers within [PSI+] aggregates with heterogeneous protein composition. Mol Biol Cell. 2008;19:2433-43 pubmed publisher
    ..Using a candidate approach, we detected Hsp104, Ssb1/2, Sis1, Sse1, Ydj1, and Sla2 among minor components of the aggregates...
  37. Skruzny M, Brach T, Ciuffa R, Rybina S, Wachsmuth M, Kaksonen M. Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis. Proc Natl Acad Sci U S A. 2012;109:E2533-42 pubmed
    ..of plasma membrane-actin cytoskeleton coupling mediated by cooperative action of epsin Ent1 and the HIP1R homolog Sla2 in yeast Saccharomyces cerevisiae...
  38. Cope M, Yang S, Shang C, Drubin D. Novel protein kinases Ark1p and Prk1p associate with and regulate the cortical actin cytoskeleton in budding yeast. J Cell Biol. 1999;144:1203-18 pubmed
    Ark1p (actin regulating kinase 1) was identified as a yeast protein that binds to Sla2p, an evolutionarily conserved cortical actin cytoskeleton protein...
  39. Holtzman D, Wertman K, Drubin D. Mapping actin surfaces required for functional interactions in vivo. J Cell Biol. 1994;126:423-32 pubmed, like a null allele of the yeast fimbrin gene (SAC6), lethal in combination with null mutations in the ABP1 and SLA2 genes, and viable in combination with a null mutation in the SLA1 gene...