Gene Symbol: SKY1
Description: serine/threonine protein kinase SKY1
Alias: serine/threonine protein kinase SKY1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Siebel C, Feng L, Guthrie C, Fu X. Conservation in budding yeast of a kinase specific for SR splicing factors. Proc Natl Acad Sci U S A. 1999;96:5440-5 pubmed
    ..The unexpected discovery of an SR protein kinase in budding yeast provides a foundation for genetic dissection of the biological functions of SR proteins and their kinases. ..
  2. Yun C, Fu X. Conserved SR protein kinase functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae. J Cell Biol. 2000;150:707-18 pubmed
  3. Erez O, Kahana C. Screening for modulators of spermine tolerance identifies Sky1, the SR protein kinase of Saccharomyces cerevisiae, as a regulator of polyamine transport and ion homeostasis. Mol Cell Biol. 2001;21:175-84 pubmed
    ..Yeast cells deleted for SKY1 developed tolerance to toxic levels of spermine, while overexpression of Sky1p in wild-type cells increased their ..
  4. Forment J, Mulet J, Vicente O, Serrano R. The yeast SR protein kinase Sky1p modulates salt tolerance, membrane potential and the Trk1,2 potassium transporter. Biochim Biophys Acta. 2002;1565:36-40 pubmed
    ..Genetic analysis and Rb(+) uptake measurements indicate that Sky1p modulates Trk1,2, the high-affinity K(+) uptake system of yeast and a major determinant of membrane potential...
  5. Dagher S, Fu X. Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4. RNA. 2001;7:1284-97 pubmed
    ..However, the function of these kinases in pre-mRNA splicing remains unclear. Here we report that SKY1, a SRPK family member in Saccharomyces cerevisiae, genetically interacts with PRP8 and PRP17/SLU4, both of which ..
  6. Schenk P, Boersma A, Brandsma J, den Dulk H, Burger H, Stoter G, et al. SKY1 is involved in cisplatin-induced cell kill in Saccharomyces cerevisiae, and inactivation of its human homologue, SRPK1, induces cisplatin resistance in a human ovarian carcinoma cell line. Cancer Res. 2001;61:6982-6 pubmed
    ..cerevisiae as a model organism to identify novel drug resistance genes, we found that disruption of the yeast gene SKY1 (serine/arginine-rich protein-specific kinase from budding yeast) by either transposon insertion or one-step gene ..
  7. Erez O, Kahana C. Deletions of SKY1 or PTK2 in the Saccharomyces cerevisiae trk1Deltatrk2Delta mutant cells exert dual effect on ion homeostasis. Biochem Biophys Res Commun. 2002;295:1142-9 pubmed
    ..We show here that deletion of SKY1 or PTK2 in trk1,2Delta cells increase spermine tolerance, implying Trk1,2p independent activity...
  8. Gilbert W, Siebel C, Guthrie C. Phosphorylation by Sky1p promotes Npl3p shuttling and mRNA dissociation. RNA. 2001;7:302-13 pubmed
    ..previously to be a substrate for the mammalian SR protein kinase, SRPK1, as well as the related yeast kinase, Sky1. Here we demonstrate that Sky1p phosphorylates only one of Npl3p's eight SR/RS dipeptides...
  9. Aubol B, Ungs L, Lukasiewicz R, Ghosh G, Adams J. Chemical clamping allows for efficient phosphorylation of the RNA carrier protein Npl3. J Biol Chem. 2004;279:30182-8 pubmed
    ..The chemical clamping mechanism offers an efficient means whereby a protein kinase can simultaneously achieve both high turnover and good substrate binding properties. ..

More Information


  1. Porat Z, Erez O, Kahana C. Cellular localization and phosphorylation of Hrb1p is independent of Sky1p. Biochim Biophys Acta. 2006;1763:207-13 pubmed
    ..Furthermore, we demonstrate that Hrb1p and additional putative Sky1p substrates, identified by computational approach, are not involved in mediating the spermine tolerant phenotype of sky1Delta cells. ..
  2. Rodríguez Lombardero S, Vizoso Vázquez A, Rodríguez Belmonte E, González Siso M, Cerdán M. SKY1 and IXR1 interactions, their effects on cisplatin and spermine resistance in Saccharomyces cerevisiae. Can J Microbiol. 2012;58:184-8 pubmed publisher
    ..Deletion of the IXR1 gene increases resistance to cisplatin, and deletion of the SKY1 gene increases resistance to cisplatin and spermine. Three S...
  3. Lukasiewicz R, Nolen B, Adams J, Ghosh G. The RGG domain of Npl3p recruits Sky1p through docking interactions. J Mol Biol. 2007;367:249-61 pubmed
    ..We suggest that docking interaction between Sky1p and Npl3p is essential for substrate recruitment and binding specificity. ..
  4. Lukasiewicz R, Velazquez Dones A, Huynh N, Hagopian J, Fu X, Adams J, et al. Structurally unique yeast and mammalian serine-arginine protein kinases catalyze evolutionarily conserved phosphorylation reactions. J Biol Chem. 2007;282:23036-43 pubmed
    ..Thus, there are physical attributes of SR and SR-like substrates that dictate the mechanism of phosphorylation, whereas the ability to processively phosphorylate substrates is inherent to SR protein kinases. ..
  5. Torabi N, Kruglyak L. Genetic basis of hidden phenotypic variation revealed by increased translational readthrough in yeast. PLoS Genet. 2012;8:e1002546 pubmed publisher
    ..We further showed that variation in SKY1, an SR protein kinase, underlies a readthrough-dependent locus observed for growth on diamide and hydrogen peroxide...