Gene Symbol: SIW14
Description: putative tyrosine protein phosphatase SIW14
Alias: OCA3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hirasaki M, Kaneko Y, Harashima S. Protein phosphatase Siw14 controls intracellular localization of Gln3 in cooperation with Npr1 kinase in Saccharomyces cerevisiae. Gene. 2008;409:34-43 pubmed publisher
    Saccharomyces cerevisiae Deltasiw14 disruptant exhibits caffeine sensitivity. To understand the function of Siw14, double disruptants for SIW14 and each of 102 viable protein kinases (PKase) genes were constructed and examined for ..
  2. Wang H, Kakaradov B, Collins S, Karotki L, Fiedler D, Shales M, et al. A complex-based reconstruction of the Saccharomyces cerevisiae interactome. Mol Cell Proteomics. 2009;8:1361-81 pubmed publisher
  3. Hirasaki M, Horiguchi M, Numamoto M, Sugiyama M, Kaneko Y, Nogi Y, et al. Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Hal5 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state. J Biosci Bioeng. 2011;111:249-54 pubmed publisher
    ..From these observations, we conclude that Ppz1 controls Gln3 localization by regulating its phosphorylation state in combination with Sat4 and Hal5. ..
  4. Armakola M, Higgins M, Figley M, Barmada S, Scarborough E, Diaz Z, et al. Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models. Nat Genet. 2012;44:1302-9 pubmed publisher
    ..Our findings provide insight into TDP-43-mediated cytotoxicity and suggest that decreasing Dbr1 activity could be a potential therapeutic approach for ALS. ..
  5. Steidle E, Chong L, Wu M, Crooke E, Fiedler D, Resnick A, et al. A Novel Inositol Pyrophosphate Phosphatase in Saccharomyces cerevisiae: Siw14 PROTEIN SELECTIVELY CLEAVES THE β-PHOSPHATE FROM 5-DIPHOSPHOINOSITOL PENTAKISPHOSPHATE (5PP-IP5). J Biol Chem. 2016;291:6772-83 pubmed publisher
    ..Purified recombinant Siw14 hydrolyzes the β-phosphate from 5-diphosphoinositol pentakisphosphate (5PP-IP5or IP7)in vitro...
  6. Binley K, Radcliffe P, Trevethick J, Duffy K, Sudbery P. The yeast PRS3 gene is required for cell integrity, cell cycle arrest upon nutrient deprivation, ion homeostasis and the proper organization of the actin cytoskeleton. Yeast. 1999;15:1459-69 pubmed
    ..In the same colony-sectoring assay, an mpk1 allele was also recovered. Multicopy PRS3 rescued the caffeine sensitivity of this mpk1 allele. ..
  7. Sakumoto N, Matsuoka I, Mukai Y, Ogawa N, Kaneko Y, Harashima S. A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis. Yeast. 2002;19:587-99 pubmed
    ..3 M CaCl(2). The synthetic phenotypes in the two latter cases where each of the PPases is categorized in a different phosphatase family led us to discuss the novel mechanism involved in the functional redundancy of the PPases. ..
  8. Romá Mateo C, Sacristán Reviriego A, Beresford N, Caparrós Martín J, Culiáñez Macià F, Martín H, et al. Phylogenetic and genetic linkage between novel atypical dual-specificity phosphatases from non-metazoan organisms. Mol Genet Genomics. 2011;285:341-54 pubmed publisher
    ..The closest yeast homolog for these proteins was the PFA-DSP from S. cerevisiae ScPFA-DSP1/Siw14/Oca3...
  9. Numamoto M, Sasano Y, Hirasaki M, Sugiyama M, Maekawa H, Harashima S. The protein phosphatase Siw14 controls caffeine-induced nuclear localization and phosphorylation of Gln3 via the type 2A protein phosphatases Pph21 and Pph22 in Saccharomyces cerevisiae. J Biochem. 2015;157:53-64 pubmed publisher
    The Saccharomyces cerevisiae Siw14, a tyrosine phosphatase involved in the response to caffeine, participates in regulation of the phosphorylation and intracellular localization of Gln3, a GATA transcriptional activator of nitrogen ..

More Information


  1. Care A, Vousden K, Binley K, Radcliffe P, Trevethick J, Mannazzu I, et al. A synthetic lethal screen identifies a role for the cortical actin patch/endocytosis complex in the response to nutrient deprivation in Saccharomyces cerevisiae. Genetics. 2004;166:707-19 pubmed
    ..Among these were mutations affecting SIW14, FEN2, SLT2, and THR4...