SIS2

Summary

Gene Symbol: SIS2
Description: phosphopantothenoylcysteine decarboxylase complex subunit SIS2
Alias: HAL3
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. pmc pH-Responsive, posttranslational regulation of the Trk1 potassium transporter by the type 1-related Ppz1 phosphatase
    Lynne Yenush
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia CSIC, Camino de Vera s n, 46022 Valencia, Spain
    Mol Cell Biol 25:8683-92. 2005
  2. doi The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities
    Cristina Molero
    Institut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Cerdanyola del Valles, Spain
    Mol Microbiol 90:367-82. 2013
  3. doi Molecular characterization of the heteromeric coenzyme A-synthesizing protein complex (CoA-SPC) in the yeast Saccharomyces cerevisiae
    Judith Olzhausen
    Institut für Genetik und Funktionelle Genomforschung, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    FEMS Yeast Res 13:565-73. 2013
  4. doi Molecular analysis of a conditional hal3 vhs3 yeast mutant links potassium homeostasis with flocculation and invasiveness
    Asier González
    Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i Biomedicina, Universitat Autonoma de Barcelona, Cerdanyola del Valles, 08193 Barcelona, Spain
    Fungal Genet Biol 53:1-9. 2013
  5. pmc Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method
    Koji Makanae
    Research Core for Interdisciplinary Sciences, Okayama University, Okayama 700 8530, Japan
    Genome Res 23:300-11. 2013
  6. doi Functional mapping of the disparate activities of the yeast moonlighting protein Hal3
    J Albert Abrie
    Department of Biochemistry, Stellenbosch University, Stellenbosch, South Africa
    Biochem J 442:357-68. 2012
  7. ncbi Functional characterization of the yeast Ppz1 phosphatase inhibitory subunit Hal3: a mutagenesis study
    Ivan Munoz
    Department de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Cerdanyola 08193, Barcelona, Spain
    J Biol Chem 279:42619-27. 2004
  8. doi Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae
    Stephanie Merchan
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia Consejo Superior de Investigaciones Cientificas, Ciudad Politécnica de Innovación, 46022 Valencia, Spain
    Genes Cells 16:152-65. 2011
  9. doi Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis
    Amparo Ruiz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Spain
    Nat Chem Biol 5:920-8. 2009
  10. pmc Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
    Peter C Stirling
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
    Mol Biol Cell 18:2336-45. 2007

Scientific Experts

  • Lynne Yenush
  • Ramon Serrano
  • Joaquin Arino
  • Asier González
  • Amparo Ruiz
  • Ivan Munoz
  • J Albert Abrie
  • Erick Strauss
  • Ernesto Simon
  • Judith Olzhausen
  • Cristina Molero
  • Koji Makanae
  • Stephanie Merchan
  • Jose Ramos
  • J Clotet
  • Anna Aksenova
  • Peter C Stirling
  • J Arino
  • Anna Barceló
  • Elena Hidalgo
  • Samuel Gelis
  • Reiko Kintaka
  • Mercè Carmona
  • Hisao Moriya
  • Hans Joachim Schüller
  • Carlos Casado
  • Tim Neetz
  • Katalin Petrényi
  • Takashi Makino
  • Hiroaki Kitano
  • Viktor Dombradi
  • Silvia Petrezselyova
  • Tom Moritz
  • E Simon
  • Guillem Hueso
  • Leda Pedelini
  • Arturo Calzada
  • R L Tatusov
  • C S Hemenway
  • Karam S Takhar
  • Andrei Pozniakovsky
  • Anthony A Hyman
  • Ludmila Mironova
  • Martin Srayko
  • Michel R Leroux
  • Kirill Volkov
  • C J Di Como
  • E de Nadal
  • J Y Masson
  • Raúl García-Salcedo
  • Maribel Marquina
  • G Rios
  • Armando Albert
  • Nuria Casals
  • Josep Clotet
  • M M Luke
  • J Ramos
  • K T Arndt
  • F Calero
  • M Y Galperin
  • D A Natale
  • E V Koonin
  • E Gari
  • J Heitman
  • M Aldea
  • N Gomez
  • D Ramotar
  • F Posas
  • A Ferrando
  • H Sugimoto
  • R Kobayashi
  • F Della Seta
  • R Bose

Detail Information

Publications26

  1. pmc pH-Responsive, posttranslational regulation of the Trk1 potassium transporter by the type 1-related Ppz1 phosphatase
    Lynne Yenush
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia CSIC, Camino de Vera s n, 46022 Valencia, Spain
    Mol Cell Biol 25:8683-92. 2005
    ..These results are consistent with a model in which the Ppz1-Hal3 interaction is a sensor of intracellular pH that modulates H+ and K+ homeostasis through the regulation of Trk1p ..
  2. doi The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities
    Cristina Molero
    Institut de Biotecnologia i Biomedicina and Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Cerdanyola del Valles, Spain
    Mol Microbiol 90:367-82. 2013
    Saccharomyces cerevisiae Hal3 and Vhs3 are moonlighting proteins, forming an atypical heterotrimeric decarboxylase (PPCDC) required for CoA biosynthesis, and regulating cation homeostasis by inhibition of the Ppz1 phosphatase...
  3. doi Molecular characterization of the heteromeric coenzyme A-synthesizing protein complex (CoA-SPC) in the yeast Saccharomyces cerevisiae
    Judith Olzhausen
    Institut für Genetik und Funktionelle Genomforschung, Ernst Moritz Arndt Universitat Greifswald, Greifswald, Germany
    FEMS Yeast Res 13:565-73. 2013
    ..In addition, nonessential genes SIS2 and VHS3 similar to CAB3 are also involved...
  4. doi Molecular analysis of a conditional hal3 vhs3 yeast mutant links potassium homeostasis with flocculation and invasiveness
    Asier González
    Departament de Bioquímica i Biologia Molecular and Institut de Biotecnologia i Biomedicina, Universitat Autonoma de Barcelona, Cerdanyola del Valles, 08193 Barcelona, Spain
    Fungal Genet Biol 53:1-9. 2013
    ..b>Hal3 and Vhs3 are moonlighting proteins acting in Saccharomyces cerevisiae both as inhibitors of the Ppz protein ..
  5. pmc Identification of dosage-sensitive genes in Saccharomyces cerevisiae using the genetic tug-of-war method
    Koji Makanae
    Research Core for Interdisciplinary Sciences, Okayama University, Okayama 700 8530, Japan
    Genome Res 23:300-11. 2013
    ..The results obtained in this study will provide basic knowledge about the physiology of chromosomal abnormalities and the evolution of chromosomal composition...
  6. doi Functional mapping of the disparate activities of the yeast moonlighting protein Hal3
    J Albert Abrie
    Department of Biochemistry, Stellenbosch University, Stellenbosch, South Africa
    Biochem J 442:357-68. 2012
    The Saccharomyces cerevisiae Hal3 protein is a moonlighting protein, able to function both as an inhibitory subunit of the Ppz1 protein phosphatase and as a constituent protomer of an unprecedented heterotrimeric PPCDC (..
  7. ncbi Functional characterization of the yeast Ppz1 phosphatase inhibitory subunit Hal3: a mutagenesis study
    Ivan Munoz
    Department de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Cerdanyola 08193, Barcelona, Spain
    J Biol Chem 279:42619-27. 2004
    Saccharomyces cerevisiae Hal3 is a conserved protein that binds the carboxyl-terminal catalytic domain of the PP1c (protein phosphatase 1)-related phosphatase Ppz1 and potently inhibits its activity, thus modulating all of the ..
  8. doi Genetic alterations leading to increases in internal potassium concentrations are detrimental for DNA integrity in Saccharomyces cerevisiae
    Stephanie Merchan
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia Consejo Superior de Investigaciones Cientificas, Ciudad Politécnica de Innovación, 46022 Valencia, Spain
    Genes Cells 16:152-65. 2011
    ....
  9. doi Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis
    Amparo Ruiz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Spain
    Nat Chem Biol 5:920-8. 2009
    ..Sequence homology analyses suggest three candidates-Ykl088w, Hal3 and Vhs3-as putative PPCDC enzymes in Saccharomyces cerevisiae...
  10. pmc Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeletal protein function and cell cycle progression
    Peter C Stirling
    Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada V5A 1S6
    Mol Biol Cell 18:2336-45. 2007
    ..Our data support a model in which Plp2p modulates the biogenesis of several CCT substrates relating to cell cycle and cytoskeletal function, which together contribute to the essential function of PLP2...
  11. ncbi The HAL3-PPZ1 dependent regulation of nonsense suppression efficiency in yeast and its influence on manifestation of the yeast prion-like determinant [ISP(+)]
    Anna Aksenova
    Department of Genetics, St Petersburg State University, 199034, St Petersburg, Russia
    Genes Cells 12:435-45. 2007
    ..In this study, we demonstrate the participation of the Hal3-Ppz1 protein complex in regulation of read-through efficiency and manifestation of non-Mendelian anti-suppressor ..
  12. ncbi Role of protein phosphatases 2C on tolerance to lithium toxicity in the yeast Saccharomyces cerevisiae
    Amparo Ruiz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Mol Microbiol 62:263-77. 2006
    ..cerevisiae, overexpression of the Ptc3p isoform resulted in increased lithium tolerance in the hypersensitive hal3 background...
  13. pmc Overexpression of SIS2, which contains an extremely acidic region, increases the expression of SWI4, CLN1 and CLN2 in sit4 mutants
    C J Di Como
    Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724 2212
    Genetics 139:95-107. 1995
    The Saccharomyces cerevisiae SIS2 gene was identified by its ability, when present on a high copy number plasmid, to increase dramatically the growth rate of sit4 mutants...
  14. ncbi Functional characterization of the Saccharomyces cerevisiae VHS3 gene: a regulatory subunit of the Ppz1 protein phosphatase with novel, phosphatase-unrelated functions
    Amparo Ruiz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    J Biol Chem 279:34421-30. 2004
    ..has been recently identified as a multicopy suppressor of the G(1)/S cell cycle blockade of a conditional sit4 and hal3 mutant...
  15. ncbi Mutagenesis analysis of the yeast Nha1 Na+/H+ antiporter carboxy-terminal tail reveals residues required for function in cell cycle
    Ernesto Simon
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    FEBS Lett 545:239-45. 2003
    ..the NHA1 gene is able to rescue the blockage at the G(1)/S transition of cells lacking Sit4 protein phosphatase and Hal3 activities...
  16. ncbi Identification of multicopy suppressors of cell cycle arrest at the G1-S transition in Saccharomyces cerevisiae
    Ivan Munoz
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona
    Yeast 20:157-69. 2003
    Inactivation of HAL3 in the absence of SIT4 function leads to cell cycle arrest at the G(1)-S transition...
  17. pmc The Ppz protein phosphatases are key regulators of K+ and pH homeostasis: implications for salt tolerance, cell wall integrity and cell cycle progression
    Lynne Yenush
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia CSIC, Camino de Vera s n, E 46022 Valencia, Spain
    EMBO J 21:920-9. 2002
    ..In addition to providing a coherent explanation for all Ppz-dependent phenotypes, our results provide evidence for a causal relationship between intracellular cation homeostasis and a potential cell cycle checkpoint...
  18. ncbi A screening for high copy suppressors of the sit4 hal3 synthetically lethal phenotype reveals a role for the yeast Nha1 antiporter in cell cycle regulation
    E Simon
    Departament de Bioquimica i Biologia Molecular, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    J Biol Chem 276:29740-7. 2001
    A screening for multicopy suppressors of the G(1)/S blockage of a conditional sit4 hal3 mutant yielded the NHA1 gene, encoding a Na(+),K(+)/H(+) antiporter, composed of a transmembrane domain and a large carboxyl-terminal tail, which has ..
  19. pmc The COG database: a tool for genome-scale analysis of protein functions and evolution
    R L Tatusov
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 28:33-6. 2000
    ..The COG database is accompanied by the COGNITOR program that is used to fit new proteins into the COGs and can be applied to functional and phylogenetic annotation of newly sequenced genomes...
  20. ncbi Lic4, a nuclear phosphoprotein that cooperates with calcineurin to regulate cation homeostasis in Saccharomyces cerevisiae
    C S Hemenway
    Department of Genetics, Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710, USA
    Mol Gen Genet 261:388-401. 1999
    ..lic4 mutations also exacerbate the Li+-sensitive phenotype of hal3 mutant strains, and overexpression of either Lic4 or Hal3 suppresses the salt sensitivity of mutant strains lacking ..
  21. pmc The yeast ser/thr phosphatases sit4 and ppz1 play opposite roles in regulation of the cell cycle
    J Clotet
    Departament de Bioquimica i Biologia Molecular, Facultat de Veterinaria, Universitat Autonoma de Barcelona, 08193 Barcelona, Spain
    Mol Cell Biol 19:2408-15. 1999
    ..The growth defect is rescued by overexpression of the HAL3/SIS2 gene, encoding a negative regulator of Ppz1...
  22. pmc The yeast halotolerance determinant Hal3p is an inhibitory subunit of the Ppz1p Ser/Thr protein phosphatase
    E de Nadal
    Departament de Bioquimica i Biologia Molecular, Fac Veterinaria, Universitat Autonoma de Barcelona, Bellaterra 08193, Barcelona, Spain
    Proc Natl Acad Sci U S A 95:7357-62. 1998
    ..Disruption or overexpression of HAL3 (also known as SIS2) has no effect on salt tolerance in the absence of PPZ1, suggesting that Hal3p might function ..
  23. pmc The transcriptional activator Imp2p maintains ion homeostasis in Saccharomyces cerevisiae
    J Y Masson
    Maisonneuve Rosemont Hospital Research Center, Universite de Montreal, Montreal, Quebec H1T 2M4, Canada
    Genetics 149:893-901. 1998
    ..Two genes that independently restored normal salt-resistance to the imp2Delta mutant, ENA1 and HAL3, were isolated...
  24. ncbi Mechanisms of salt tolerance conferred by overexpression of the HAL1 gene in Saccharomyces cerevisiae
    G Rios
    Instituto de Biologia Molecular y Celular de Plantas, Universidad Politecnica de Valencia CSIC, Spain
    Yeast 13:515-28. 1997
    ..As a consequence, osmotic adjustment through glycerolsynthesis, a process not affected by HAL1, is the limiting factor for growth on galactose under NaCl stressed...
  25. pmc The SAP, a new family of proteins, associate and function positively with the SIT4 phosphatase
    M M Luke
    Cold Spring Harbor Laboratory, New York 11724 2212, USA
    Mol Cell Biol 16:2744-55. 1996
    ..Overexpression of a SAP from one group does not suppress the defects due to the loss of the other group. These findings and others indicate that the SAPs have distinct functions...