Gene Symbol: SIP1
Description: Sip1p
Alias: Sip1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Orlova M, Kanter E, Krakovich D, Kuchin S. Nitrogen availability and TOR regulate the Snf1 protein kinase in Saccharomyces cerevisiae. Eukaryot Cell. 2006;5:1831-7 pubmed
    ..TOR and Snf1/AMPK are highly conserved in evolution, and their novel functional interaction in yeast suggests similar mechanisms in other eukaryotes. ..
  2. Nath N, McCartney R, Schmidt M. Purification and characterization of Snf1 kinase complexes containing a defined Beta subunit composition. J Biol Chem. 2002;277:50403-8 pubmed
    The Snf1 kinase complex of Saccharomyces cerevisiae contains one of three possible beta subunits encoded by either SIP1, SIP2, or GAL83...
  3. McCartney R, Garnar Wortzel L, Chandrashekarappa D, Schmidt M. Activation and inhibition of Snf1 kinase activity by phosphorylation within the activation loop. Biochim Biophys Acta. 2016;1864:1518-28 pubmed publisher
    ..The first is through direct dephosphorylation of the conserved activation loop threonine. The second is through phosphorylation of serine 214. ..
  4. Mizuno T, Masuda Y, Irie K. The Saccharomyces cerevisiae AMPK, Snf1, Negatively Regulates the Hog1 MAPK Pathway in ER Stress Response. PLoS Genet. 2015;11:e1005491 pubmed publisher
    ..Thus, Snf1 plays pleiotropic roles in ER stress response by negatively regulating the Hog1 MAPK pathway and the UPR pathway. ..
  5. Nazarenus T, Cedarberg R, Bell R, Cheatle J, Forch A, Haifley A, et al. Upf1p, a highly conserved protein required for nonsense-mediated mRNA decay, interacts with the nuclear pore proteins Nup100p and Nup116p. Gene. 2005;345:199-212 pubmed
  6. Shields C, Taylor R, Nazarenus T, Cheatle J, Hou A, Tapprich A, et al. Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis. Curr Genet. 2003;44:184-94 pubmed
    ..Our results suggest Ats1p might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p. ..
  7. Long R, Hopper J. Genetic and carbon source regulation of phosphorylation of Sip1p, a Snf1p-associated protein involved in carbon response in Saccharomyces cerevisiae. Yeast. 1995;11:233-46 pubmed
    ..We have carried out in vivo studies of the genetic and carbon control of Sip1p phosphorylation...
  8. Chandrashekarappa D, McCartney R, O Donnell A, Schmidt M. The ? subunit of yeast AMP-activated protein kinase directs substrate specificity in response to alkaline stress. Cell Signal. 2016;28:1881-1893 pubmed publisher
    Saccharomyces cerevisiae express three isoforms of Snf1 kinase that differ by which ? subunit is present, Gal83, Sip1 or Sip2...
  9. Zhang J, Olsson L, Nielsen J. The beta-subunits of the Snf1 kinase in Saccharomyces cerevisiae, Gal83 and Sip2, but not Sip1, are redundant in glucose derepression and regulation of sterol biosynthesis. Mol Microbiol. 2010;77:371-83 pubmed publisher
    ..of a catalytic alpha-subunit Snf1, a regulatory gamma-subunit Snf4 and one of three possible beta-subunits, Sip1, Sip2 or Gal83...

More Information


  1. Dubacq C, Chevalier A, Mann C. The protein kinase Snf1 is required for tolerance to the ribonucleotide reductase inhibitor hydroxyurea. Mol Cell Biol. 2004;24:2560-72 pubmed
    ..Quantitative regulation of Snf1 kinase activity may contribute to the specificity of the effector responses that it controls. ..
  2. Vyas V, Kuchin S, Berkey C, Carlson M. Snf1 kinases with different beta-subunit isoforms play distinct roles in regulating haploid invasive growth. Mol Cell Biol. 2003;23:1341-8 pubmed
    ..Cells contain three forms of the Snf1 kinase, each with a different beta-subunit isoform, either Gal83, Sip1, or Sip2...
  3. Launay J, Birraux G, Bondoux D, Callebert J, Choi D, Loric S, et al. Ras involvement in signal transduction by the serotonin 5-HT2B receptor. J Biol Chem. 1996;271:3141-7 pubmed
  4. Yang X, Jiang R, Carlson M. A family of proteins containing a conserved domain that mediates interaction with the yeast SNF1 protein kinase complex. EMBO J. 1994;13:5878-86 pubmed
    ..Previously, we identified SIP1 and SIP2 as proteins that interact with SNF1 in vivo by the two-hybrid system...
  5. Rutter J, Probst B, McKnight S. Coordinate regulation of sugar flux and translation by PAS kinase. Cell. 2002;111:17-28 pubmed
    ..These studies provide evidence of a cell-autonomous signaling system that both controls and connects the balance of fuel consumption/storage to protein synthesis. ..
  6. Vincent O, Townley R, Kuchin S, Carlson M. Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism. Genes Dev. 2001;15:1104-14 pubmed
    ..Snf1 kinase complexes contain the alpha (catalytic) subunit Snf1, one of the three related beta subunits Gal83, Sip1, or Sip2, and the gamma subunit Snf4...
  7. Mylin L, Bushman V, Long R, Yu X, Lebo C, Blank T, et al. SIP1 is a catabolite repression-specific negative regulator of GAL gene expression. Genetics. 1994;137:689-700 pubmed
    ..Snf1p is known to associate with several proteins. One is Sip1p, a protein that becomes phosphorylated in the presence of Snf1p and thus is a candidate Snf1p kinase substrate...
  8. Li L, Kaplan J, Ward D. The glucose sensor Snf1 and the transcription factors Msn2 and Msn4 regulate transcription of the vacuolar iron importer gene CCC1 and iron resistance in yeast. J Biol Chem. 2017;292:15577-15586 pubmed publisher
    ..In conclusion, we show that yeast have developed multiple transcriptional mechanisms to regulate Ccc1 expression and to protect against high cytosolic iron toxicity. ..
  9. Schmidt M, McCartney R. beta-subunits of Snf1 kinase are required for kinase function and substrate definition. EMBO J. 2000;19:4936-43 pubmed
    ..Saccharomyces cerevisiae encodes three beta-subunit genes, SIP1, SIP2 and GAL83. Earlier studies suggested that these subunits may not be required for Snf1 kinase function...
  10. Jiang R, Carlson M. The Snf1 protein kinase and its activating subunit, Snf4, interact with distinct domains of the Sip1/Sip2/Gal83 component in the kinase complex. Mol Cell Biol. 1997;17:2099-106 pubmed
    ..Here, we examine the possibility that a family of proteins, comprising Sip1, Sip2, and Gal83, serves this purpose...
  11. Backhaus K, Rippert D, Heilmann C, Sorgo A, de Koster C, Klis F, et al. Mutations in SNF1 complex genes affect yeast cell wall strength. Eur J Cell Biol. 2013;92:383-95 pubmed publisher
    ..Epistasis analyses with mutants in cell wall integrity (CWI) signaling confirm that the SNF1 complex and the CWI pathway independently affect yeast cell integrity. ..
  12. Yang X, Hubbard E, Carlson M. A protein kinase substrate identified by the two-hybrid system. Science. 1992;257:680-2 pubmed
    ..One of the genes, SIP1, was independently isolated as a multicopy suppressor of defects caused by reduced SNF1 kinase activity, and ..
  13. Kimura Y, Irie K, Mizuno T. Expression control of the AMPK regulatory subunit and its functional significance in yeast ER stress response. Sci Rep. 2017;7:46713 pubmed publisher
    ..However, it remains unclear how the alternate three ? subunits, Sip1, Sip2, and Gal83, of the Snf1 complex participate in ER stress response...
  14. Hedbacker K, Hong S, Carlson M. Pak1 protein kinase regulates activation and nuclear localization of Snf1-Gal83 protein kinase. Mol Cell Biol. 2004;24:8255-63 pubmed
    ..of the activating kinases for the three forms of Snf1 protein kinase containing the beta-subunit isoforms Gal83, Sip1, and Sip2...
  15. Elbing K, McCartney R, Schmidt M. Purification and characterization of the three Snf1-activating kinases of Saccharomyces cerevisiae. Biochem J. 2006;393:797-805 pubmed
    ..Finally, we showed that the Snf1 kinase domain isolated from bacteria as a GST fusion protein can be activated in vitro and shows substrate specificity in the absence of its beta and gamma subunits. ..
  16. Erickson J, Johnston M. Genetic and molecular characterization of GAL83: its interaction and similarities with other genes involved in glucose repression in Saccharomyces cerevisiae. Genetics. 1993;135:655-64 pubmed
    ..The sequence of the predicted Gal83 protein is homologous to two other yeast proteins, Sip1p and Sip2p, which are known to interact with the SNF1 gene product, a protein kinase required for expression of the ..
  17. McCartney R, Chandrashekarappa D, Zhang B, Schmidt M. Genetic analysis of resistance and sensitivity to 2-deoxyglucose in Saccharomyces cerevisiae. Genetics. 2014;198:635-46 pubmed publisher
    ..Snf1 kinase activated by 2-deoxyglucose does not phosphorylate the Mig1 protein, a known Snf1 substrate during glucose limitation. Thus, different stimuli elicit distinct responses from the Snf1 kinase. ..