SGS1

Summary

Gene Symbol: SGS1
Description: ATP-dependent DNA helicase SGS1
Alias: ATP-dependent DNA helicase SGS1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Prakash R, Satory D, Dray E, Papusha A, Scheller J, Kramer W, et al. Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination. Genes Dev. 2009;23:67-79 pubmed publisher
    ..Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over...
  2. Gangloff S, McDonald J, Bendixen C, Arthur L, Rothstein R. The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol Cell Biol. 1994;14:8391-8 pubmed
    ..We identified a mutation, sgs1 (slow growth suppressor), that suppresses both the growth defect and the increased genomic instability of top3 ..
  3. Oh S, Lao J, Hwang P, Taylor A, Smith G, Hunter N. BLM ortholog, Sgs1, prevents aberrant crossing-over by suppressing formation of multichromatid joint molecules. Cell. 2007;130:259-72 pubmed
    ..We show that the Saccharomyces cerevisiae BLM ortholog, Sgs1, prevents aberrant crossing-over during meiosis by suppressing formation of joint molecules (JMs) comprising three ..
  4. Vijeh Motlagh N, Seki M, Branzei D, Enomoto T. Mgs1 and Rad18/Rad5/Mms2 are required for survival of Saccharomyces cerevisiae mutants with novel temperature/cold sensitive alleles of the DNA polymerase delta subunit, Pol31. DNA Repair (Amst). 2006;5:1459-74 pubmed
    ..Deletion of SGS1, RAD52, SRS2, MRC1 or RAD24 had a deleterious effect only in combination with those pol31 alleles that had a ..
  5. Kats E, Albuquerque C, Zhou H, Kolodner R. Checkpoint functions are required for normal S-phase progression in Saccharomyces cerevisiae RCAF- and CAF-I-defective mutants. Proc Natl Acad Sci U S A. 2006;103:3710-5 pubmed
    ..Our data suggest that RCAF defects lead to unstable replication forks that are then stabilized by replication checkpoint proteins, whereas CAF-I defects likely cause different types of DNA damage. ..
  6. Karras G, Fumasoni M, Sienski G, Vanoli F, Branzei D, Jentsch S. Noncanonical role of the 9-1-1 clamp in the error-free DNA damage tolerance pathway. Mol Cell. 2013;49:536-46 pubmed publisher
    ..Our findings thus reveal unexpected cooperation in the error-free pathway between the two related clamps and indicate that 9-1-1 plays a broader role in the DNA damage response than previously assumed. ..
  7. Zakharyevich K, Tang S, Ma Y, Hunter N. Delineation of joint molecule resolution pathways in meiosis identifies a crossover-specific resolvase. Cell. 2012;149:334-47 pubmed publisher
    ..Paradoxically, crossing over in this background is strongly dependent on the Blooms helicase ortholog Sgs1, a component of a well-characterized anticrossover activity...
  8. Bennett R, Keck J, Wang J. Binding specificity determines polarity of DNA unwinding by the Sgs1 protein of S. cerevisiae. J Mol Biol. 1999;289:235-48 pubmed
    Saccharomyces cerevisiae Sgs1 protein is a member of the RecQ DNA helicase family which also includes the products of the human Bloom's syndrome and Werner's syndrome genes...
  9. Karras G, Jentsch S. The RAD6 DNA damage tolerance pathway operates uncoupled from the replication fork and is functional beyond S phase. Cell. 2010;141:255-67 pubmed publisher
    ..We therefore propose that the RAD6 pathway acts on single-stranded gaps left behind newly restarted replication forks. ..

More Information

Publications94

  1. Cejka P, Kowalczykowski S. The full-length Saccharomyces cerevisiae Sgs1 protein is a vigorous DNA helicase that preferentially unwinds holliday junctions. J Biol Chem. 2010;285:8290-301 pubmed publisher
    The highly conserved RecQ family of DNA helicases has multiple roles in the maintenance of genome stability. Sgs1, the single RecQ homologue in Saccharomyces cerevisiae, acts both early and late during homologous recombination...
  2. Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, Delgado Cruzata L, et al. RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J. 2005;24:2024-33 pubmed
    b>SGS1 encodes a DNA helicase whose homologues in human cells include the BLM, WRN, and RECQ4 genes, mutations in which lead to cancer-predisposition syndromes...
  3. Ii M, Ii T, Brill S. Mus81 functions in the quality control of replication forks at the rDNA and is involved in the maintenance of rDNA repeat number in Saccharomyces cerevisiae. Mutat Res. 2007;625:1-19 pubmed
    Previous studies in yeast have suggested that the SGS1 DNA helicase or the Mus81-Mms4 structure-specific endonuclease is required to suppress the accumulation of lethal recombination intermediates during DNA replication...
  4. Budd M, Campbell J. The pattern of sensitivity of yeast dna2 mutants to DNA damaging agents suggests a role in DSB and postreplication repair pathways. Mutat Res. 2000;459:173-86 pubmed
    ..b>SGS1 encodes a helicase with similar properties to Dna2 protein...
  5. Onodera R, Seki M, Ui A, Satoh Y, Miyajima A, Onoda F, et al. Functional and physical interaction between Sgs1 and Top3 and Sgs1-independent function of Top3 in DNA recombination repair. Genes Genet Syst. 2002;77:11-21 pubmed
    A mutant allele of SGS1 of Saccharomyces cerevisiae was identified as a suppressor of the slow-growth phenotype of top3 mutants...
  6. Sacher M, Pfander B, Hoege C, Jentsch S. Control of Rad52 recombination activity by double-strand break-induced SUMO modification. Nat Cell Biol. 2006;8:1284-90 pubmed
    ..Furthermore, our data indicate that sumoylation becomes particularly relevant for those Rad52 molecules that are engaged in recombination. ..
  7. Wang T, Kung W. Supercomplex formation between Mlh1-Mlh3 and Sgs1-Top3 heterocomplexes in meiotic yeast cells. Biochem Biophys Res Commun. 2002;296:949-53 pubmed
    ..In this report, we show that yeast Mlh3 co-immunoprecipitates with Sgs1 helicase in sporulating cells at late stage of meiotic prophase I...
  8. Kaliraman V, Brill S. Role of SGS1 and SLX4 in maintaining rDNA structure in Saccharomyces cerevisiae. Curr Genet. 2002;41:389-400 pubmed
    To address the role of SGS1 in controlling genome stability we previously identified several slx mutants that require SGS1 for viability...
  9. Lillard Wetherell K, Combs K, Groden J. BLM helicase complements disrupted type II telomere lengthening in telomerase-negative sgs1 yeast. Cancer Res. 2005;65:5520-2 pubmed
    ..The RecQ-like helicases, BLM and Sgs1, are implicated in recombination-mediated telomere lengthening in human cells and budding yeast, respectively...
  10. Nag D, Cavallo S. Effects of mutations in SGS1 and in genes functionally related to SGS1 on inverted repeat-stimulated spontaneous unequal sister-chromatid exchange in yeast. BMC Mol Biol. 2007;8:120 pubmed publisher
    ..The RecQ helicase Sgs1, in Saccharomyces cerevisiae, is believed to act on stalled replication forks...
  11. Gravel S, Chapman J, Magill C, Jackson S. DNA helicases Sgs1 and BLM promote DNA double-strand break resection. Genes Dev. 2008;22:2767-72 pubmed publisher
    ..absence of exonuclease Exo1 activity, deletion or mutation of the Saccharomyces cerevisiae RecQ-family helicase, Sgs1, causes pronounced hypersensitivity to DSB-inducing agents...
  12. Mankouri H, Ngo H, Hickson I. Esc2 and Sgs1 act in functionally distinct branches of the homologous recombination repair pathway in Saccharomyces cerevisiae. Mol Biol Cell. 2009;20:1683-94 pubmed publisher
    ..These roles are qualitatively similar to those of Sgs1, the yeast ortholog of the human Bloom's syndrome protein, BLM...
  13. Marrero V, Symington L. Extensive DNA end processing by exo1 and sgs1 inhibits break-induced replication. PLoS Genet. 2010;6:e1001007 pubmed publisher
    ..The rare chromosome rearrangements due to BIR template switching at repeated sequences were increased in the exo1Delta sgs1Delta mutant, suggesting that reduced resection can decrease the fidelity of homologous recombination. ..
  14. Gallo Fernández M, Saugar I, Ortiz Bazan M, Vázquez M, Tercero J. Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4. Nucleic Acids Res. 2012;40:8325-35 pubmed
    ..mms4 mutant shows highly reduced nuclease activity and increases the sensitivity of cells lacking the RecQ-helicase Sgs1 to various agents that cause DNA damage or replicative stress...
  15. Bennett R, Noirot Gros M, Wang J. Interaction between yeast sgs1 helicase and DNA topoisomerase III. J Biol Chem. 2000;275:26898-905 pubmed
    The Saccharomyces cerevisiae Sgs1 protein is a member of the RecQ family of DNA helicases that includes the human Bloom's syndrome and Werner's syndrome proteins...
  16. Mimitou E, Symington L. Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. EMBO J. 2010;29:3358-69 pubmed publisher
    ..In contrast, both the Exo1 and Sgs1 resection pathways contribute to DSB processing in the absence of Ku and Sae2 or when the MRX complex is intact, ..
  17. Chavez A, Agrawal V, Johnson F. Homologous recombination-dependent rescue of deficiency in the structural maintenance of chromosomes (Smc) 5/6 complex. J Biol Chem. 2011;286:5119-25 pubmed publisher
    ..Recent work has highlighted a role for Smc5/6 and the Sgs1 DNA helicase in preventing the accumulation of unresolved HR intermediates...
  18. Weitao T, Budd M, Campbell J. Evidence that yeast SGS1, DNA2, SRS2, and FOB1 interact to maintain rDNA stability. Mutat Res. 2003;532:157-72 pubmed
    ..Our current work shows that mutation inactivating Sgs1, the yeast RecQ helicase ortholog, also causes accumulation of stalled replication forks and DSBs at the rDNA RFB...
  19. Ii M, Brill S. Roles of SGS1, MUS81, and RAD51 in the repair of lagging-strand replication defects in Saccharomyces cerevisiae. Curr Genet. 2005;48:213-25 pubmed
    Yeast cells lacking the SGS1 DNA helicase and the MUS81 structure-specific endonuclease display a synthetic lethality that is suppressed by loss of the RAD51 recombinase...
  20. Cohen H, Sinclair D. Recombination-mediated lengthening of terminal telomeric repeats requires the Sgs1 DNA helicase. Proc Natl Acad Sci U S A. 2001;98:3174-9 pubmed
    The Saccharomyces cerevisiae SGS1 gene encodes a RecQ-like DNA helicase, human homologues of which are implicated in the genetic instability disorders, Bloom syndrome (BS), Rothmund-Thomson syndrome (RTS), and Werner syndrome (WS)...
  21. Sollier J, Driscoll R, Castellucci F, Foiani M, Jackson S, Branzei D. The Saccharomyces cerevisiae Esc2 and Smc5-6 proteins promote sister chromatid junction-mediated intra-S repair. Mol Biol Cell. 2009;20:1671-82 pubmed publisher
    ..RecQ helicases, including yeast Sgs1 and human BLM, safeguard genome integrity through their functions in DNA recombination...
  22. Myung K, Kolodner R. Suppression of genome instability by redundant S-phase checkpoint pathways in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2002;99:4500-7 pubmed
    ..Combinations of mutations causing individual defects in the RAD24 or SGS1 branches of the intra-S checkpoint or the replication checkpoint showed synergistic interactions with regard to the ..
  23. Bonetti D, Clerici M, Manfrini N, Lucchini G, Longhese M. The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends. PLoS ONE. 2010;5:e14142 pubmed publisher
    ..of DNA ends, which is specifically inhibited during the G1 cell cycle phase, requires the MRX complex, Sae2, Sgs1 and Exo1...
  24. Mankouri H, Ashton T, Hickson I. Holliday junction-containing DNA structures persist in cells lacking Sgs1 or Top3 following exposure to DNA damage. Proc Natl Acad Sci U S A. 2011;108:4944-9 pubmed publisher
    The Sgs1-Rmi1-Top3 "dissolvasome" is required for the maintenance of genome stability and has been implicated in the processing of various types of DNA structures arising during DNA replication...
  25. Szakal B, Branzei D. Premature Cdk1/Cdc5/Mus81 pathway activation induces aberrant replication and deleterious crossover. EMBO J. 2013;32:1155-67 pubmed publisher
    ..The Sgs1-Top3- and Mus81-Mms4-dependent resolution pathways occupy different temporal windows in relation to replication, ..
  26. Bonetti D, Martina M, Clerici M, Lucchini G, Longhese M. Multiple pathways regulate 3' overhang generation at S. cerevisiae telomeres. Mol Cell. 2009;35:70-81 pubmed publisher
    ..We show by an inducible short telomere assay that Sae2 and the Sgs1 RecQ helicase control two distinct but partially complementary pathways for nucleolytic processing of S...
  27. Bjergbaek L, Cobb J, Tsai Pflugfelder M, Gasser S. Mechanistically distinct roles for Sgs1p in checkpoint activation and replication fork maintenance. EMBO J. 2005;24:405-17 pubmed
    ..In this function, the Sgs1p/Top3p complex acts in parallel to the Claspin-related adaptor, Mrc1p, although the sgs1 and mrc1 mutations are epistatic for Rad53p activation...
  28. Klein H. Mutations in recombinational repair and in checkpoint control genes suppress the lethal combination of srs2Delta with other DNA repair genes in Saccharomyces cerevisiae. Genetics. 2001;157:557-65 pubmed
    ..However, cells do not achieve wild-type growth rates, suggesting that unrepaired damage is still present and may lead to chromosome loss. ..
  29. Myung K, Datta A, Chen C, Kolodner R. SGS1, the Saccharomyces cerevisiae homologue of BLM and WRN, suppresses genome instability and homeologous recombination. Nat Genet. 2001;27:113-6 pubmed
    The Escherichia coli gene recQ was identified as a RecF recombination pathway gene. The gene SGS1, encoding the only RecQ-like DNA helicase in Saccharomyces cerevisiae, was identified by mutations that suppress the top3 slow-growth ..
  30. Zhu Z, Chung W, Shim E, Lee S, Ira G. Sgs1 helicase and two nucleases Dna2 and Exo1 resect DNA double-strand break ends. Cell. 2008;134:981-94 pubmed publisher
    ..We show that the Mre11-Rad50-Xrs2 complex (MRX) initiates 5' degradation, whereas Sgs1 and Dna2 degrade 5' strands exposing long 3' strands...
  31. Chin J, Bashkirov V, Heyer W, Romesberg F. Esc4/Rtt107 and the control of recombination during replication. DNA Repair (Amst). 2006;5:618-28 pubmed
    ..Thus, we propose that Esc4 associates with ssDNA of stalled forks and acts as a scaffolding protein to recruit and/or modulate the function of other proteins required to reinitiate DNA synthesis. ..
  32. Azam M, Lee J, Abraham V, Chanoux R, Schoenly K, Johnson F. Evidence that the S.cerevisiae Sgs1 protein facilitates recombinational repair of telomeres during senescence. Nucleic Acids Res. 2006;34:506-16 pubmed
    ..b>sgs1 and rad52 mutations are epistatic during senescence, indicating that Sgs1p participates in a RAD52-dependent ..
  33. Matos J, Blanco M, West S. Cell-cycle kinases coordinate the resolution of recombination intermediates with chromosome segregation. Cell Rep. 2013;4:76-86 pubmed publisher
    ..Therefore, temporal restriction of JM resolution, imposed by Cdk/Cdc5, minimizes the potential for loss of heterozygosity while preventing chromosome missegregation and aneuploidy. ..
  34. Ui A, Satoh Y, Onoda F, Miyajima A, Seki M, Enomoto T. The N-terminal region of Sgs1, which interacts with Top3, is required for complementation of MMS sensitivity and suppression of hyper-recombination in sgs1 disruptants. Mol Genet Genomics. 2001;265:837-50 pubmed
    The SGS1 gene of Saccharomyces (cerevisiae is a homologue of the genes affected in Bloom's syndrome, Werner's syndrome, and Rothmund-Thomson's syndrome...
  35. Lu C, Tsai C, Brill S, Teng S. Sumoylation of the BLM ortholog, Sgs1, promotes telomere-telomere recombination in budding yeast. Nucleic Acids Res. 2010;38:488-98 pubmed publisher
    ..As the only RecQ DNA helicase in the yeast Saccharomyces cerevisiae, Sgs1 is known to safeguard genome integrity through its role in DNA recombination...
  36. Tay Y, Sidebotham J, Wu L. Mph1 requires mismatch repair-independent and -dependent functions of MutSalpha to regulate crossover formation during homologous recombination repair. Nucleic Acids Res. 2010;38:1889-901 pubmed publisher
    ..reveal an MMR-independent function of MutSalpha in generating COs that is specifically antagonized by Mph1, but not Sgs1. In contrast, both Mph1 and MutSalpha are required to efficiently suppress COs in the presence of a homeologous ..
  37. Ngo H, Lydall D. Survival and growth of yeast without telomere capping by Cdc13 in the absence of Sgs1, Exo1, and Rad9. PLoS Genet. 2010;6:e1001072 pubmed publisher
    ..We found that simultaneous inactivation of Sgs1, Exo1, and Rad9, three DNA damage response (DDR) proteins, is sufficient to allow cell division in the absence of ..
  38. Dayani Y, Simchen G, Lichten M. Meiotic recombination intermediates are resolved with minimal crossover formation during return-to-growth, an analogue of the mitotic cell cycle. PLoS Genet. 2011;7:e1002083 pubmed publisher
    ..RTG in sgs1-?C795 mutants, which lack the helicase and Holliday junction-binding domains of this BLM homolog, led to a ..
  39. Schmidt K, Kolodner R. Suppression of spontaneous genome rearrangements in yeast DNA helicase mutants. Proc Natl Acad Sci U S A. 2006;103:18196-201 pubmed
    Saccharomyces cerevisiae mutants lacking two of the three DNA helicases Sgs1, Srs2, and Rrm3 exhibit slow growth that is suppressed by disrupting homologous recombination...
  40. Chen Y, Choi K, Szakal B, Arenz J, Duan X, Ye H, et al. Interplay between the Smc5/6 complex and the Mph1 helicase in recombinational repair. Proc Natl Acad Sci U S A. 2009;106:21252-7 pubmed publisher
    ..does not alleviate sensitivity to DNA damage or the accumulation of recombination intermediates in cells lacking Sgs1, which is thought to function together with the Smc5/6 complex...
  41. Bennett R, Sharp J, Wang J. Purification and characterization of the Sgs1 DNA helicase activity of Saccharomyces cerevisiae. J Biol Chem. 1998;273:9644-50 pubmed
    The yeast Saccharomyces cerevisiae Sgs1 protein is a member of a family of DNA helicases that include the Escherichia coli RecQ protein and the products of human Bloom's syndrome and Werner's syndrome genes...
  42. Miyajima A, Seki M, Onoda F, Shiratori M, Odagiri N, Ohta K, et al. Sgs1 helicase activity is required for mitotic but apparently not for meiotic functions. Mol Cell Biol. 2000;20:6399-409 pubmed
    The SGS1 gene of Saccharomyces cerevisiae is a homologue for the Bloom's syndrome and Werner's syndrome genes...
  43. Dherin C, Gueneau E, Francin M, Nunez M, Miron S, Liberti S, et al. Characterization of a highly conserved binding site of Mlh1 required for exonuclease I-dependent mismatch repair. Mol Cell Biol. 2009;29:907-18 pubmed publisher
    ..In this study, we identified the site of yeast Mlh1 critical for the interaction with Exo1, Ntg2, and Sgs1 proteins, designated as site S2 by reference to the Mlh1/Pms1 heterodimerization site S1...
  44. Oh S, Lao J, Taylor A, Smith G, Hunter N. RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, resolve aberrant joint molecules during meiotic recombination. Mol Cell. 2008;31:324-36 pubmed publisher
    Saccharomyces cerevisiae RecQ helicase, Sgs1, and XPF family endonuclease, Mus81-Mms4, are implicated in processing joint molecule (JM) recombination intermediates...
  45. Schmidt K, Wu J, Kolodner R. Control of translocations between highly diverged genes by Sgs1, the Saccharomyces cerevisiae homolog of the Bloom's syndrome protein. Mol Cell Biol. 2006;26:5406-20 pubmed
    b>Sgs1 is a RecQ family DNA helicase required for genome stability in Saccharomyces cerevisiae whose human homologs BLM, WRN, and RECQL4 are mutated in Bloom's, Werner, and Rothmund Thomson syndromes, respectively...
  46. Jessop L, Lichten M. Mus81/Mms4 endonuclease and Sgs1 helicase collaborate to ensure proper recombination intermediate metabolism during meiosis. Mol Cell. 2008;31:313-23 pubmed publisher
    Budding yeast lacking the Sgs1 helicase and the Mus81/Mms4 endonuclease are inviable, and indirect studies implicate homologous recombination gone awry as the cause of death...
  47. Saffi J, Feldmann H, Winnacker E, Henriques J. Interaction of the yeast Pso5/Rad16 and Sgs1 proteins: influences on DNA repair and aging. Mutat Res. 2001;486:195-206 pubmed
    ..One of the interactors found was Sgs1, a DNA helicase influencing the life span of Saccharomyces cerevisiae, with homology to the human BLM, WRN and ..
  48. Schürer K, Rudolph C, Ulrich H, Kramer W. Yeast MPH1 gene functions in an error-free DNA damage bypass pathway that requires genes from Homologous recombination, but not from postreplicative repair. Genetics. 2004;166:1673-86 pubmed
    ..On the contrary, in an sgs1 background we found a pronounced hyperrecombination phenotype...
  49. Huang P, Pryde F, Lester D, Maddison R, Borts R, Hickson I, et al. SGS1 is required for telomere elongation in the absence of telomerase. Curr Biol. 2001;11:125-9 pubmed
    ..We show here that the telomere elongation pathway in yeast (type II) is dependent on SGS1, the yeast homolog of the gene products of Werner's (WRN) and Bloom's (BLM) syndromes...
  50. Bastin Shanower S, Fricke W, Mullen J, Brill S. The mechanism of Mus81-Mms4 cleavage site selection distinguishes it from the homologous endonuclease Rad1-Rad10. Mol Cell Biol. 2003;23:3487-96 pubmed
    ..Genetically, we show that both mus81 and sgs1 mutants are sensitive to camptothecin-induced DNA damage...
  51. Ooi S, Shoemaker D, Boeke J. DNA helicase gene interaction network defined using synthetic lethality analyzed by microarray. Nat Genet. 2003;35:277-86 pubmed
    ..We used SGS1 and SRS2, two 3'-->5' DNA helicase genes, as 'queries' to identify their redundant and unique biological ..
  52. Lee S, Johnson R, Yu S, Prakash L, Prakash S. Requirement of yeast SGS1 and SRS2 genes for replication and transcription. Science. 1999;286:2339-42 pubmed
    The SGS1 gene of the yeast Saccharomyces cerevisiae encodes a DNA helicase with homology to the human Bloom's syndrome gene BLM and the Werner's syndrome gene WRN. The SRS2 gene of yeast also encodes a DNA helicase...
  53. Sun H, Bennett R, Maizels N. The Saccharomyces cerevisiae Sgs1 helicase efficiently unwinds G-G paired DNAs. Nucleic Acids Res. 1999;27:1978-84 pubmed
    ..Diminished ability to unwind G-G paired regions may also explain the deleterious effect of mutation of Sgs1 on rDNA stability, and the accelerated aging characteristic of yeast strains that lack Sgs1 as well as humans ..
  54. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..RPA directly participates in DNA double-strand break repair by stimulating 5'-3' end resection by the Sgs1/BLM helicase and Dna2 endonuclease in vitro...
  55. Gangloff S, Soustelle C, Fabre F. Homologous recombination is responsible for cell death in the absence of the Sgs1 and Srs2 helicases. Nat Genet. 2000;25:192-4 pubmed
    ..In the yeast Saccharomyces cerevisiae, the absence of the Sgs1 helicase results in genomic instability and accelerated ageing...
  56. Ashton T, Mankouri H, Heidenblut A, McHugh P, Hickson I. Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. Mol Cell Biol. 2011;31:1921-33 pubmed publisher
    The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex...
  57. Chang M, Bellaoui M, Boone C, Brown G. A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proc Natl Acad Sci U S A. 2002;99:16934-9 pubmed
    ..These genes may promote replication fork stability or processivity during encounters between replication forks and DNA damage. ..
  58. Foster S, Balestrini A, Petrini J. Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage. Mol Cell Biol. 2011;31:4379-89 pubmed publisher
    ..Collectively, the data define a nonhomologous end joining (NHEJ)-independent, S-phase-specific function of the Ku heterodimer. ..
  59. Sinclair D, Mills K, Guarente L. Accelerated aging and nucleolar fragmentation in yeast sgs1 mutants. Science. 1997;277:1313-6 pubmed
    The SGS1 gene of yeast encodes a DNA helicase with homology to the human WRN gene. Mutations in WRN result in Werner's syndrome, a disease with symptoms resembling premature aging...
  60. Dunø M, Thomsen B, Westergaard O, Krejci L, Bendixen C. Genetic analysis of the Saccharomyces cerevisiae Sgs1 helicase defines an essential function for the Sgs1-Top3 complex in the absence of SRS2 or TOP1. Mol Gen Genet. 2000;264:89-97 pubmed
    The Saccharomyces cerevisiae gene SGS1 encodes a DNA helicase that shows homology to the Escherichia coli protein RecQ and the products of the BLM and WRN genes in humans, which are defective in Bloom's and Werner's syndrome, respectively...
  61. Fabre F, Chan A, Heyer W, Gangloff S. Alternate pathways involving Sgs1/Top3, Mus81/ Mms4, and Srs2 prevent formation of toxic recombination intermediates from single-stranded gaps created by DNA replication. Proc Natl Acad Sci U S A. 2002;99:16887-92 pubmed
    ..For example, mutations affecting both the Srs2 and Sgs1 helicases result in extremely poor growth, a phenotype suppressed by mutations in genes that govern early stages of ..
  62. Mitchel K, Lehner K, Jinks Robertson S. Heteroduplex DNA position defines the roles of the Sgs1, Srs2, and Mph1 helicases in promoting distinct recombination outcomes. PLoS Genet. 2013;9:e1003340 pubmed publisher
    The contributions of the Sgs1, Mph1, and Srs2 DNA helicases during mitotic double-strand break (DSB) repair in yeast were investigated using a gap-repair assay...
  63. Putnam C, Hayes T, Kolodner R. Post-replication repair suppresses duplication-mediated genome instability. PLoS Genet. 2010;6:e1000933 pubmed publisher
    ..The SRS2, SGS1, and HCS1 encoded helicases appeared to interact with Rad5, and epistasis analysis suggested that Srs2 and Hcs1 act ..
  64. Chen C, Brill S. An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs. EMBO J. 2010;29:1713-25 pubmed publisher
    ..BLM is highly conserved and all BLM orthologs, including budding yeast Sgs1, have a large N-terminus that binds Top3-Rmi1 but has no known catalytic activity...
  65. Rockmill B, Voelkel Meiman K, Roeder G. Centromere-proximal crossovers are associated with precocious separation of sister chromatids during meiosis in Saccharomyces cerevisiae. Genetics. 2006;174:1745-54 pubmed
    ..Strains mutant for the RecQ homolog, SGS1, display reduced spore viability and increased crossing over...
  66. Bernstein K, Shor E, Sunjevaric I, Fumasoni M, Burgess R, Foiani M, et al. Sgs1 function in the repair of DNA replication intermediates is separable from its role in homologous recombinational repair. EMBO J. 2009;28:915-25 pubmed publisher
    ..The budding yeast Saccharomyces cerevisiae has one RecQ helicase, Sgs1, which functions with Top3 and Rmi1 in DNA repair...
  67. Lydeard J, Lipkin Moore Z, Jain S, Eapen V, Haber J. Sgs1 and exo1 redundantly inhibit break-induced replication and de novo telomere addition at broken chromosome ends. PLoS Genet. 2010;6:e1000973 pubmed publisher
    ..Resection proceeds by two pathways dependent on Exo1 or the BLM homolog, Sgs1. We report that Exo1 and Sgs1 each inhibit BIR but have little effect on GC, while overexpression of either protein ..
  68. Mimitou E, Symington L. Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing. Nature. 2008;455:770-4 pubmed publisher
    ..Here we demonstrate that yeast Exo1 nuclease and Sgs1 helicase function in alternative pathways for DSB processing...
  69. Chen X, Cui D, Papusha A, Zhang X, Chu C, Tang J, et al. The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends. Nature. 2012;489:576-80 pubmed publisher
    ..resection have been described both in cells and in vitro, one relying on Exo1 exonuclease and the other on Sgs1 helicase and Dna2 nuclease...
  70. Kerrest A, Anand R, Sundararajan R, Bermejo R, Liberi G, Dujon B, et al. SRS2 and SGS1 prevent chromosomal breaks and stabilize triplet repeats by restraining recombination. Nat Struct Mol Biol. 2009;16:159-67 pubmed publisher
    ..These molecules migrate similarly to reversed replication forks, and their presence is dependent on SRS2 and SGS1 but not RAD51...
  71. Schmidt K, Kolodner R. Requirement of Rrm3 helicase for repair of spontaneous DNA lesions in cells lacking Srs2 or Sgs1 helicase. Mol Cell Biol. 2004;24:3213-26 pubmed
    ..Here, we show that rrm3 srs2 and rrm3 sgs1 mutants, in which two different DNA helicases have been inactivated, exhibit a severe growth defect and undergo ..
  72. Mullen J, Nallaseth F, Lan Y, Slagle C, Brill S. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol. 2005;25:4476-87 pubmed
    ..requires a set of RecQ-Top3 DNA helicase-topoisomerase complexes whose sole budding yeast homolog is encoded by SGS1-TOP3...
  73. Mischo H, Gómez González B, Grzechnik P, Rondon A, Wei W, Steinmetz L, et al. Yeast Sen1 helicase protects the genome from transcription-associated instability. Mol Cell. 2011;41:21-32 pubmed publisher
    ..Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability. ..
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    b>Sgs1, the budding yeast homolog of the mammalian BLM helicase, has been implicated in preventing excess recombination during both vegetative growth and meiosis...
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    ..Similarly, mutation in SGS1, the RECQ homologue in budding yeast, results in genomic instability and accelerated ageing...
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    ..In light of these results, we propose a model in which R-loop-mediated recombination is explained by template switching. ..
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    ..DNA end-resection machinery comprising the Saccharomyces cerevisiae Mre11-Rad50-Xrs2 (MRX) complex, the Sgs1-Top3-Rmi1 complex, Dna2 protein and the heterotrimeric ssDNA-binding protein RPA...
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    ..In the budding yeast Saccharomyces cerevisiae, mutations in the RecQ homolog, SGS1, phenocopy many of the defects observed in the human syndromes...
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    ..We propose that the helix-destabilizing activity of RPA channels ssDNA intermediates from mutagenic MMEJ to error-free homologous recombination, thus preserving genome integrity. ..
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    ..Thus, the DSB resection and procrossover functions of Exo1 during meiosis involve temporally and biochemically distinct activities. ..
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    ..We suggest that Cdk1 kinase counteracts unscheduled sumoylation of Srs2 and targets Srs2 to dismantle specific DNA structures, such as the D-loops, in a helicase-dependent manner during homologous recombinational repair. ..
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    Mutation in SGS1, which encodes the yeast homolog of the human Bloom helicase, or in mismatch repair (MMR) genes confers defects in the suppression of mitotic recombination between similar but nonidentical (homeologous) sequences...
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    ..Here we show that the Saccharomyces cerevisiae Sgs1 and Top3 proteins physically interact in cell extracts and bind directly in vitro...
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    The Saccharomyces cerevisiae SGS1 gene is homologous to Escherichia coli RecQ and the human BLM and WRN proteins that are defective in the cancer-prone disorder Bloom's syndrome and the premature aging disorder Werner's syndrome, ..