Gene Symbol: SEN1
Description: putative DNA/RNA helicase SEN1
Alias: CIK3, NRD2
Species: Saccharomyces cerevisiae S288c
Products:     SEN1

Top Publications

  1. Nedea E, Nalbant D, Xia D, Theoharis N, Suter B, Richardson C, et al. The Glc7 phosphatase subunit of the cleavage and polyadenylation factor is essential for transcription termination on snoRNA genes. Mol Cell. 2008;29:577-87 pubmed publisher
    ..Overexpressing a C-terminal fragment of Sen1, a superfamily I helicase required for snoRNA termination, suppresses the growth and termination defects associated ..
  2. Rondon A, Mischo H, Kawauchi J, Proudfoot N. Fail-safe transcriptional termination for protein-coding genes in S. cerevisiae. Mol Cell. 2009;36:88-98 pubmed publisher
    ..We propose that these alternative Pol II termination pathways serve the dual function of avoiding transcription interference and promoting rapid removal of aberrant transcripts. ..
  3. Kawauchi J, Mischo H, Braglia P, Rondon A, Proudfoot N. Budding yeast RNA polymerases I and II employ parallel mechanisms of transcriptional termination. Genes Dev. 2008;22:1082-92 pubmed publisher
  4. Porrúa O, Libri D. A bacterial-like mechanism for transcription termination by the Sen1p helicase in budding yeast. Nat Struct Mol Biol. 2013;20:884-91 pubmed publisher
    ..We also show that termination is inhibited by RNA-DNA hybrids. Our results elucidate the role of Sen1p in controlling pervasive transcription. ..
  5. Kim H, Choe J, Seo Y. The sen1(+) gene of Schizosaccharomyces pombe, a homologue of budding yeast SEN1, encodes an RNA and DNA helicase. Biochemistry. 1999;38:14697-710 pubmed
    ..Determination of the partial amino acid sequence of the 95-kDa enzyme revealed that it is encoded by the sen1(+)() gene, an S...
  6. Vasiljeva L, Kim M, Mutschler H, Buratowski S, Meinhart A. The Nrd1-Nab3-Sen1 termination complex interacts with the Ser5-phosphorylated RNA polymerase II C-terminal domain. Nat Struct Mol Biol. 2008;15:795-804 pubmed publisher
    ..To study how a mechanism is chosen, we analyzed recruitment of Nrd1, which cooperates with Nab3 and Sen1 to terminate small nucleolar RNAs and other short RNAs...
  7. Mischo H, Gómez González B, Grzechnik P, Rondon A, Wei W, Steinmetz L, et al. Yeast Sen1 helicase protects the genome from transcription-associated instability. Mol Cell. 2011;41:21-32 pubmed publisher
    b>Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts...
  8. Ursic D, Himmel K, Gurley K, Webb F, Culbertson M. The yeast SEN1 gene is required for the processing of diverse RNA classes. Nucleic Acids Res. 1997;25:4778-85 pubmed
    A single base change in the helicase superfamily 1 domain of the yeast Saccharomyces cerevisiae SEN1 gene results in a heat-sensitive mutation that alters the cellular abundance of many RNA species...
  9. Ursic D, Chinchilla K, Finkel J, Culbertson M. Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing. Nucleic Acids Res. 2004;32:2441-52 pubmed
    ..The protein-protein and protein-RNA interactions reported here suggest that the DNA/RNA helicase activity of Sen1p is utilized for several different purposes in multiple gene expression pathways. ..

More Information


  1. Vasiljeva L, Buratowski S. Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts. Mol Cell. 2006;21:239-48 pubmed
    ..We find that the RNA binding protein Nrd1, complexed with its partners Nab3, Sen1, and cap binding complex, physically interacts with the nuclear form of exosome...
  2. Steinmetz E, Warren C, Kuehner J, Panbehi B, Ansari A, Brow D. Genome-wide distribution of yeast RNA polymerase II and its control by Sen1 helicase. Mol Cell. 2006;24:735-746 pubmed publisher
    ..We compared a wild-type strain with a strain bearing a substitution in the Sen1 helicase, which is a Pol II termination factor for noncoding RNA genes...
  3. Ursic D, Demarini D, Culbertson M. Inactivation of the yeast Sen1 protein affects the localization of nucleolar proteins. Mol Gen Genet. 1995;249:571-84 pubmed
    A mutation in the Saccharomyces cerevisiae SEN1 gene causes accumulation of end-matured, intron-containing pre-tRNAs. Cells containing the thermosensitive sen1-1 mutation exhibit reduced tRNA splicing endonuclease activity...
  4. Porrúa O, Hobor F, Boulay J, Kubicek K, d Aubenton Carafa Y, Gudipati R, et al. In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1-dependent transcription termination. EMBO J. 2012;31:3935-48 pubmed publisher
    The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs...
  5. Finkel J, Chinchilla K, Ursic D, Culbertson M. Sen1p performs two genetically separable functions in transcription and processing of U5 small nuclear RNA in Saccharomyces cerevisiae. Genetics. 2010;184:107-18 pubmed publisher
    The Saccharomyces cerevisiae SEN1 gene codes for a nuclear-localized superfamily I helicase...
  6. Jamonnak N, Creamer T, Darby M, Schaughency P, Wheelan S, Corden J. Yeast Nrd1, Nab3, and Sen1 transcriptome-wide binding maps suggest multiple roles in post-transcriptional RNA processing. RNA. 2011;17:2011-25 pubmed publisher
    ..cerevisiae are terminated through a pathway directed by the RNA-binding proteins Nrd1, Nab3, and the RNA helicase Sen1. We have used an in vivo cross-linking approach to map the binding sites of components of the yeast non-poly(A) ..
  7. Steinmetz E, Conrad N, Brow D, Corden J. RNA-binding protein Nrd1 directs poly(A)-independent 3'-end formation of RNA polymerase II transcripts. Nature. 2001;413:327-31 pubmed
    ..The RNA-binding protein Nab3, the putative RNA helicase Sen1, and the intact C-terminal domain of pol II are also required for efficient response to the element...
  8. Singh V, Azad G, Sariki S, Tomar R. Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p. FEBS Lett. 2015;589:3165-74 pubmed publisher
    The Nrd1-Nab3-Sen1 (NNS) complex terminates transcription of non-coding RNA genes and mediates degradation of the produced transcript by the nuclear exosome...
  9. Chinchilla K, Rodríguez Molina J, Ursic D, Finkel J, Ansari A, Culbertson M. Interactions of Sen1, Nrd1, and Nab3 with multiple phosphorylated forms of the Rpb1 C-terminal domain in Saccharomyces cerevisiae. Eukaryot Cell. 2012;11:417-29 pubmed publisher
    The Saccharomyces cerevisiae SEN1 gene codes for a nuclear, ATP-dependent helicase which is embedded in a complex network of protein-protein interactions...
  10. Chen X, Poorey K, Carver M, Müller U, Bekiranov S, Auble D, et al. Transcriptomes of six mutants in the Sen1 pathway reveal combinatorial control of transcription termination across the Saccharomyces cerevisiae genome. PLoS Genet. 2017;13:e1006863 pubmed publisher be similar to that of mRNAs, but a distinct pathway is utilized for termination of most non-coding RNAs: the Sen1-dependent or "NNS" pathway. Here, we examine the effect on the S...
  11. Legros P, Malapert A, Niinuma S, Bernard P, Vanoosthuyse V. RNA processing factors Swd2.2 and Sen1 antagonize RNA Pol III-dependent transcription and the localization of condensin at Pol III genes. PLoS Genet. 2014;10:e1004794 pubmed publisher
    ..2 and Sen1, two factors involved in the transcription process, are simultaneously deleted...
  12. Beggs S, James T, Bond U. The PolyA tail length of yeast histone mRNAs varies during the cell cycle and is influenced by Sen1p and Rrp6p. Nucleic Acids Res. 2012;40:2700-11 pubmed publisher
    ..Thus, histone mRNAs are distinct from the general pool of yeast mRNAs and 3'-end processing and polyadenylation contribute to the cell cycle regulation of these transcripts. ..
  13. Leonaitė B, Han Z, Basquin J, Bonneau F, Libri D, Porrúa O, et al. Sen1 has unique structural features grafted on the architecture of the Upf1-like helicase family. EMBO J. 2017;36:1590-1604 pubmed publisher
    The superfamily 1B (SF1B) helicase Sen1 is an essential protein that plays a key role in the termination of non-coding transcription in yeast...
  14. Golla U, Singh V, Azad G, Singh P, Verma N, Mandal P, et al. Sen1p contributes to genomic integrity by regulating expression of ribonucleotide reductase 1 (RNR1) in Saccharomyces cerevisiae. PLoS ONE. 2013;8:e64798 pubmed publisher
    ..Moreover, SEN1 mutants [Sen1-1 (G1747D), Sen1-2 (?1-975)] exhibited defects in DNA damage checkpoint activation...
  15. Han Z, Libri D, Porrúa O. Biochemical characterization of the helicase Sen1 provides new insights into the mechanisms of non-coding transcription termination. Nucleic Acids Res. 2017;45:1355-1370 pubmed publisher
    ..In Saccharomyces cerevisiae, the highly conserved helicase Sen1 plays a key role in restricting pervasive transcription by eliciting early termination of non-coding transcription...
  16. Kim K, Heo D, Kim I, Suh J, Kim M. Exosome Cofactors Connect Transcription Termination to RNA Processing by Guiding Terminated Transcripts to the Appropriate Exonuclease within the Nuclear Exosome. J Biol Chem. 2016;291:13229-42 pubmed publisher
    ..This suggests that Mpp6 and Trf4 may play a role in choosing a particular RNA processing route for Nrd1-terminated transcripts within the exosome by guiding the transcripts to the appropriate exonuclease. ..
  17. Heo D, Yoo I, Kong J, Lidschreiber M, Mayer A, Choi B, et al. The RNA polymerase II C-terminal domain-interacting domain of yeast Nrd1 contributes to the choice of termination pathway and couples to RNA processing by the nuclear exosome. J Biol Chem. 2013;288:36676-90 pubmed publisher
    ..Thus, the Nrd1 CID couples Ser(P)-5 CTD not only to termination, but also to RNA processing by the nuclear exosome. ..
  18. Grzechnik P, Gdula M, Proudfoot N. Pcf11 orchestrates transcription termination pathways in yeast. Genes Dev. 2015;29:849-61 pubmed publisher
    ..RNA (ncRNA) generated by RNA polymerase II (Pol II) are terminated by the NRD complex consisting of Nrd1, Nab3, and Sen1. We now show that Pcf11, a component of the cleavage and polyadenylation complex (CPAC), is also generally required ..
  19. Terzi N, Churchman L, Vasiljeva L, Weissman J, Buratowski S. H3K4 trimethylation by Set1 promotes efficient termination by the Nrd1-Nab3-Sen1 pathway. Mol Cell Biol. 2011;31:3569-83 pubmed publisher
    In Saccharomyces cerevisiae, the Nrd1-Nab3-Sen1 pathway mediates the termination of snoRNAs and cryptic unstable transcripts (CUTs)...
  20. Brandina I, Smirnov A, Kolesnikova O, Entelis N, Krasheninnikov I, Martin R, et al. tRNA import into yeast mitochondria is regulated by the ubiquitin-proteasome system. FEBS Lett. 2007;581:4248-54 pubmed
    ..This result suggests a functional link between UPS and tRNA mitochondrial import in yeast and indicates on the existence of negative and positive import regulators. ..
  21. Kim K, Levin D. Mpk1 MAPK association with the Paf1 complex blocks Sen1-mediated premature transcription termination. Cell. 2011;144:745-56 pubmed publisher
    ..this interaction causes a specific defect in transcription elongation of an Mpk1-induced gene, which results from Sen1-dependent premature termination through a Nab3-binding site within the promoter-proximal region of the gene...
  22. Hazelbaker D, Marquardt S, Wlotzka W, Buratowski S. Kinetic competition between RNA Polymerase II and Sen1-dependent transcription termination. Mol Cell. 2013;49:55-66 pubmed publisher
    The essential helicase-like protein Sen1 mediates termination of RNA Polymerase II (Pol II) transcription at snoRNAs and other noncoding RNAs in yeast...
  23. Martin Tumasz S, Brow D. Saccharomyces cerevisiae Sen1 Helicase Domain Exhibits 5'- to 3'-Helicase Activity with a Preference for Translocation on DNA Rather than RNA. J Biol Chem. 2015;290:22880-9 pubmed publisher
    In the yeast Saccharomyces cerevisiae, the essential nuclear helicase Sen1 is required for efficient termination of transcription of short noncoding RNA genes by RNA polymerase II...
  24. Goossens A, de La Fuente N, Forment J, Serrano R, Portillo F. Regulation of yeast H(+)-ATPase by protein kinases belonging to a family dedicated to activation of plasma membrane transporters. Mol Cell Biol. 2000;20:7654-61 pubmed
  25. Chen X, Müller U, Sundling K, Brow D. Saccharomyces cerevisiae Sen1 as a model for the study of mutations in human Senataxin that elicit cerebellar ataxia. Genetics. 2014;198:577-90 pubmed publisher
    The nuclear RNA and DNA helicase Sen1 is essential in the yeast Saccharomyces cerevisiae and is required for efficient termination of RNA polymerase II transcription of many short noncoding RNA genes...
  26. Li W, Selvam K, Rahman S, Li S. Sen1, the yeast homolog of human senataxin, plays a more direct role than Rad26 in transcription coupled DNA repair. Nucleic Acids Res. 2016;44:6794-802 pubmed publisher
    ..known to play an important role in transcription coupled DNA repair (TCR) in the yeast Saccharomyces cerevisiae Sen1, a DNA/RNA helicase that is essential for yeast cell viability and homologous to human senataxin, has been known to ..
  27. Sariki S, Sahu P, Golla U, Singh V, Azad G, Tomar R. Sen1, the homolog of human Senataxin, is critical for cell survival through regulation of redox homeostasis, mitochondrial function, and the TOR pathway in Saccharomyces cerevisiae. FEBS J. 2016;283:4056-4083 pubmed publisher
    ..we first show alterations in key cellular pathways such as: redox, unfolded protein response, and TOR in the yeast sen1 ?N mutant (N-terminal truncation)...
  28. Vasiljeva L, Kim M, Terzi N, Soares L, Buratowski S. Transcription termination and RNA degradation contribute to silencing of RNA polymerase II transcription within heterochromatin. Mol Cell. 2008;29:313-23 pubmed publisher
    ..These transcripts are terminated by the Nrd1/Sen1 complex and degraded by the exosome...
  29. Alzu A, Bermejo R, Begnis M, Lucca C, Piccini D, Carotenuto W, et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell. 2012;151:835-846 pubmed publisher
    ..We show that the Sen1/Senataxin DNA/RNA helicase associates with forks, promoting their progression across RNA polymerase II (RNAPII)-..