SEC9

Summary

Gene Symbol: SEC9
Description: Sec9p
Alias: HSS7, Sec9p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hattendorf D, Andreeva A, Gangar A, Brennwald P, Weis W. Structure of the yeast polarity protein Sro7 reveals a SNARE regulatory mechanism. Nature. 2007;446:567-71 pubmed
    ..Deletion of the Sro7 tail enables binding to the Qbc SNARE region of Sec9 and this interaction inhibits SNARE complex assembly...
  2. Munson M, Hughson F. Conformational regulation of SNARE assembly and disassembly in vivo. J Biol Chem. 2002;277:9375-81 pubmed
    ..membrane SNARE Sso1p sequesters the C-terminal SNARE motif and prevents it from binding to its assembly partners Sec9p and Sncp. Here, we introduce mutations into Sso1p that cause it to remain constitutively open...
  3. Wiederkehr A, De Craene J, Ferro Novick S, Novick P. Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol. 2004;167:875-87 pubmed
    ..Furthermore, a fraction of Sec1p can be coprecipitated with the exoycst. Our results suggest that Sec1p couples exocyst-mediated vesicle tethering with SNARE-mediated docking and fusion. ..
  4. Zhang X, Wang P, Gangar A, Zhang J, Brennwald P, TerBush D, et al. Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis. J Cell Biol. 2005;170:273-83 pubmed
    ..We propose that, although Lgl is broadly distributed in the cells, its localized interaction with the exocyst and kinetic activation are important for the establishment and reenforcement of cell polarity. ..
  5. Knop M, Miller K, Mazza M, Feng D, Weber M, Keränen S, et al. Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast. Mol Biol Cell. 2005;16:4543-56 pubmed
    ..Mso1p coimmunoprecipitates with Sec1p, Sso1/2p, Snc1/2p, Sec9p, and the exocyst complex subunit Sec15p...
  6. Weber Boyvat M, Aro N, Chernov K, Nyman T, Jantti J. Sec1p and Mso1p C-terminal tails cooperate with the SNAREs and Sec4p in polarized exocytosis. Mol Biol Cell. 2011;22:230-44 pubmed publisher
    ..The results show that the Sec1p tail binds preferentially ternary Sso1p-Sec9p-Snc2p complexes and it enhances ternary SNARE complex formation in vitro...
  7. Castillo Flores A, Weinberger A, Robinson M, Gerst J. Mso1 is a novel component of the yeast exocytic SNARE complex. J Biol Chem. 2005;280:34033-41 pubmed
    ..exocytic SNARE complex consists of one molecule each of the Sso1/2 target SNAREs, Snc1/2 vesicular SNAREs, and the Sec9 target SNARE, which form a fusion complex that is conserved in evolution...
  8. Lustgarten V, Gerst J. Yeast VSM1 encodes a v-SNARE binding protein that may act as a negative regulator of constitutive exocytosis. Mol Cell Biol. 1999;19:4480-94 pubmed
    ..In contrast, VSM1 overexpression in cells which bear a temperature-sensitive mutation in the Sec9 t-SNARE (sec9-4 cells) results in the accumulation of non-invertase-containing low-density secretory vesicles, ..
  9. Fiebig K, Rice L, Pollock E, Brunger A. Folding intermediates of SNARE complex assembly. Nat Struct Biol. 1999;6:117-23 pubmed
    ..states of a yeast SNARE complex: first, the 'closed' conformation of Sso1; second, the binary complex of Sso1 and Sec9; and third, the ternary complex of Sso1, Sec9 and Snc1. Sec9 and Snc1 are unstructured in isolation...

More Information

Publications61

  1. Songer J, Munson M. Sec6p anchors the assembled exocyst complex at sites of secretion. Mol Biol Cell. 2009;20:973-82 pubmed publisher
    ..Our results indicate that assembly and polarization of the exocyst are functionally separable events, and that Sec6p is required to anchor exocyst complexes at sites of secretion. ..
  2. Scott B, Van Komen J, Irshad H, Liu S, Wilson K, McNew J. Sec1p directly stimulates SNARE-mediated membrane fusion in vitro. J Cell Biol. 2004;167:75-85 pubmed
    ..These data strongly argue that Sec1p directly stimulates SNARE-mediated membrane fusion. ..
  3. Sivaram M, Saporita J, Furgason M, Boettcher A, Munson M. Dimerization of the exocyst protein Sec6p and its interaction with the t-SNARE Sec9p. Biochemistry. 2005;44:6302-11 pubmed
    ..Moreover, we found that the dimer of Sec6p binds to the plasma membrane t-SNARE Sec9p and inhibits the interaction between Sec9p and its partner t-SNARE Sso1p...
  4. McNew J, Parlati F, Fukuda R, Johnston R, Paz K, Paumet F, et al. Compartmental specificity of cellular membrane fusion encoded in SNARE proteins. Nature. 2000;407:153-9 pubmed
    ..Here we find that, to a marked degree, the pattern of membrane flow in the cell is encoded and recapitulated by its isolated SNARE proteins, as predicted by the SNARE hypothesis. ..
  5. Aalto M, Ronne H, Keranen S. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J. 1993;12:4095-104 pubmed
    ..number can suppress sec1 mutations and also mutations in several other late acting SEC genes, such as SEC3, SEC5, SEC9 and SEC15. SSO1 and SSO2 encode small proteins with N-terminal hydrophilic domains and C-terminal hydrophobic tails...
  6. Lehman K, Rossi G, Adamo J, Brennwald P. Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. J Cell Biol. 1999;146:125-40 pubmed
    ..yeast proteins, Sro7p and Sro77p, based on their ability to bind to the plasma membrane SNARE (SNARE) protein, Sec9p. These proteins show significant similarity to the Drosophila tumor suppressor, lethal giant larvae and to the ..
  7. Morgera F, Sallah M, Dubuke M, Gandhi P, Brewer D, Carr C, et al. Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1. Mol Biol Cell. 2012;23:337-46 pubmed publisher
    ..complexes and membrane fusion through interactions between the Sec6 subunit and the plasma membrane SNARE protein Sec9. Here we show another facet of Sec6 function-it directly binds Sec1, another SNARE regulator, but of the Sec1/..
  8. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  9. Brennwald P, Kearns B, Champion K, Keranen S, Bankaitis V, Novick P. Sec9 is a SNAP-25-like component of a yeast SNARE complex that may be the effector of Sec4 function in exocytosis. Cell. 1994;79:245-58 pubmed
    ..The most potent of these was found to be SEC9, a gene required for post-Golgi transport...
  10. Katz L, Hanson P, Heuser J, Brennwald P. Genetic and morphological analyses reveal a critical interaction between the C-termini of two SNARE proteins and a parallel four helical arrangement for the exocytic SNARE complex. EMBO J. 1998;17:6200-9 pubmed
    In a screen for suppressors of a temperature-sensitive mutation in the yeast SNAP-25 homolog, Sec9, we have identified a gain-of-function mutation in the yeast synaptobrevin homolog, Snc2...
  11. Togneri J, Cheng Y, Munson M, Hughson F, Carr C. Specific SNARE complex binding mode of the Sec1/Munc-18 protein, Sec1p. Proc Natl Acad Sci U S A. 2006;103:17730-5 pubmed
    ..We propose that vesicle fusion requires a specific interaction between the SM protein and the ternary SNARE complex. ..
  12. Carr C, Grote E, Munson M, Hughson F, Novick P. Sec1p binds to SNARE complexes and concentrates at sites of secretion. J Cell Biol. 1999;146:333-44 pubmed
    ..that yeast Sec1p coprecipitates not only the syntaxin homologue Ssop, but also the other two exocytic SNAREs (Sec9p and Sncp) in amounts and in proportions characteristic of SNARE complexes in yeast lysates...
  13. Aalto M, Jantti J, Ostling J, Keranen S, Ronne H. Mso1p: a yeast protein that functions in secretion and interacts physically and genetically with Sec1p. Proc Natl Acad Sci U S A. 1997;94:7331-6 pubmed
    ..These findings suggest that Mso1p is a component of the secretory vesicle docking complex whose function is closely associated with that of Sec1p. ..
  14. Elbert M, Rossi G, Brennwald P. The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery. Mol Biol Cell. 2005;16:532-49 pubmed
    ..We show that Kin1 and Kin2 physically interact with the t-SNARE Sec9 and the Lgl homologue Sro7, proteins acting at the final stage of exocytosis...
  15. Grosshans B, Andreeva A, Gangar A, Niessen S, Yates J, Brennwald P, et al. The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. J Cell Biol. 2006;172:55-66 pubmed
    ..Furthermore, we demonstrate the formation of a ternary complex of Sec4-GTP, Sro7p, and the t-SNARE Sec9p. Genetic data support our conclusion that Sro7p functions downstream of Sec4p and further imply that Sro7p and the ..
  16. Govindan B, Bowser R, Novick P. The role of Myo2, a yeast class V myosin, in vesicular transport. J Cell Biol. 1995;128:1055-68 pubmed
    ..Our observations are consistent with a role for Myo2 in transporting a class of secretory vesicles from the mother cell along actin cables into the bud. ..
  17. Adamo J, Moskow J, Gladfelter A, Viterbo D, Lew D, Brennwald P. Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud. J Cell Biol. 2001;155:581-92 pubmed
    ..Rather, we suggest that Cdc42 acts as an allosteric regulator of the vesicle docking and fusion apparatus to provide maximal function at sites of polarized growth. ..
  18. Rossi G, Salminen A, Rice L, Brunger A, Brennwald P. Analysis of a yeast SNARE complex reveals remarkable similarity to the neuronal SNARE complex and a novel function for the C terminus of the SNAP-25 homolog, Sec9. J Biol Chem. 1997;272:16610-7 pubmed
    ..of a nonneuronal SNARE complex using recombinant forms of the yeast exocytic SNARE proteins Snc1, Sso1, and Sec9 and the yeast alpha-SNAP homolog, Sec17...
  19. Hutagalung A, Coleman J, Pypaert M, Novick P. An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components. Mol Biol Cell. 2009;20:153-63 pubmed publisher
    ..The results suggest that either Exo70p or Sec3p must associate with the plasma membrane for the exocyst to function as a vesicle tether. ..
  20. Weber M, Chernov K, Turakainen H, Wohlfahrt G, Pajunen M, Savilahti H, et al. Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1. Mol Biol Cell. 2010;21:1362-74 pubmed publisher
    ..We show that the putative N-peptide binding area in Sec1p domain 1 is important for Mso1p binding, and that Mso1p can interact with Sso1p and Sso2p. Our results suggest that Mso1p mimics N-peptide binding to facilitate membrane fusion. ..
  21. Hashizume K, Cheng Y, Hutton J, Chiu C, Carr C. Yeast Sec1p functions before and after vesicle docking. Mol Biol Cell. 2009;20:4673-85 pubmed publisher
  22. Jantti J, Aalto M, Oyen M, Sundqvist L, Keränen S, Ronne H. Characterization of temperature-sensitive mutations in the yeast syntaxin 1 homologues Sso1p and Sso2p, and evidence of a distinct function for Sso1p in sporulation. J Cell Sci. 2002;115:409-20 pubmed
    ..suppressors of sso2-1 yielded three genes that are involved in the terminal step of secretion: SNC1, SNC2 and SEC9. The sso1-1 mutation interacts synthetically with a disruption of the MSO1 gene, which encodes a Sec1p interacting ..
  23. Nicholson K, Munson M, Miller R, Filip T, Fairman R, Hughson F. Regulation of SNARE complex assembly by an N-terminal domain of the t-SNARE Sso1p. Nat Struct Biol. 1998;5:793-802 pubmed
    ..Binary complexes form between the target membrane SNAREs Sso1p and Sec9p; these binary complexes can subsequently bind to the vesicle SNARE Snc2p to form ternary complexes...
  24. Gangar A, Rossi G, Andreeva A, Hales R, Brennwald P. Structurally conserved interaction of Lgl family with SNAREs is critical to their cellular function. Curr Biol. 2005;15:1136-42 pubmed
    ..on the yeast Lgl homologs, Sro7/Sro77, suggest a function in exocytosis through interaction with the t-SNARE Sec9. Using yeast/mammalian Lgl chimeras, we demonstrate that the overall architecture of Lgl proteins is highly ..
  25. Yakir Tamang L, Gerst J. A phosphatidylinositol-transfer protein and phosphatidylinositol-4-phosphate 5-kinase control Cdc42 to regulate the actin cytoskeleton and secretory pathway in yeast. Mol Biol Cell. 2009;20:3583-97 pubmed publisher
    ..Based upon these findings, we propose that the exocytic signal involves PI delivery to the PI kinases (i.e., Mss4) by Sfh5, generation of PI(4,5)P(2), and PI(4,5)P(2)-dependent regulation of Cdc42 and the actin cytoskeleton. ..
  26. Gabriely G, Kama R, Gelin Licht R, Gerst J. Different domains of the UBL-UBA ubiquitin receptor, Ddi1/Vsm1, are involved in its multiple cellular roles. Mol Biol Cell. 2008;19:3625-37 pubmed publisher
    ..g., Ddi1(T346A), Ddi1(T348A)) are unable to facilitate growth of the sec9-4 t-SNARE mutant. In contrast, the overproduction of phosphorylatable forms of Ddi1 (e.g...
  27. Roth D, Guo W, Novick P. Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell. 1998;9:1725-39 pubmed
  28. Grote E, Baba M, Ohsumi Y, Novick P. Geranylgeranylated SNAREs are dominant inhibitors of membrane fusion. J Cell Biol. 2000;151:453-66 pubmed
    ..factor attachment protein receptor (v-SNARE) Sncp and the plasma membrane t-SNAREs Ssop and Sec9p into a SNARE complex...
  29. Walch Solimena C, Novick P. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat Cell Biol. 1999;1:523-5 pubmed
  30. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, et al. The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol. 2013;202:509-26 pubmed publisher
    ..We propose that the exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins. This could help to confer both specificity and directionality to vesicular traffic. ..
  31. Van Komen J, Bai X, Rodkey T, Schaub J, McNew J. The polybasic juxtamembrane region of Sso1p is required for SNARE function in vivo. Eukaryot Cell. 2005;4:2017-28 pubmed
    ..in Saccharomyces cerevisiae requires the specific interaction between the plasma membrane t-SNARE complex (Sso1/2p;Sec9p)and a vesicular v-SNARE (Snc1/2p)...
  32. Marash M, Gerst J. t-SNARE dephosphorylation promotes SNARE assembly and exocytosis in yeast. EMBO J. 2001;20:411-21 pubmed
    ..Sso t-SNARE dephosphorylation correlated with its assembly into complexes with the Sec9 t-SNARE, both in vitro and in vivo, and with an increase in protein trafficking and secretion in cells...
  33. Gerst J. Conserved alpha-helical segments on yeast homologs of the synaptobrevin/VAMP family of v-SNAREs mediate exocytic function. J Biol Chem. 1997;272:16591-8 pubmed
    ..secretion competence to snc cells and to interact genetically with components of the proposed fusion complex: the Sec9 and Sso2 t-SNAREs and the Sec17 alpha-SNAP homolog...
  34. Liu S, Wilson K, Rice Stitt T, Neiman A, McNew J. In vitro fusion catalyzed by the sporulation-specific t-SNARE light-chain Spo20p is stimulated by phosphatidic acid. Traffic. 2007;8:1630-43 pubmed
    b>Sec9p and Spo20p are two SNAP25 family SNARE proteins specialized for different developmental stages in yeast...
  35. Gao X, Albert S, Tcheperegine S, Burd C, Gallwitz D, Bi E. The GAP activity of Msb3p and Msb4p for the Rab GTPase Sec4p is required for efficient exocytosis and actin organization. J Cell Biol. 2003;162:635-46 pubmed
    ..Using a strain defective in polarized secretion and actin-patch organization, we showed that a change in actin-patch organization could be a consequence of the fusion of mistargeted vesicles with the plasma membrane. ..
  36. Rossi G, Watson K, Demonch M, Temple B, Brennwald P. In vitro reconstitution of Rab GTPase-dependent vesicle clustering by the yeast lethal giant larvae/tomosyn homolog, Sro7. J Biol Chem. 2015;290:612-24 pubmed publisher
    ..Consistent with this, we show that occupancy of the N terminus of Sro7 by the t-SNARE Sec9, which results in the open conformation of Sro7, also acts to inhibit vesicle cluster formation by Sro7...
  37. Cleves A, Novick P, Bankaitis V. Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function. J Cell Biol. 1989;109:2939-50 pubmed
    ..Moreover, these sac1 suppressor properties also extended to sec6 and sec9 secretory vesicle defects...
  38. Marash M, Gerst J. Phosphorylation of the autoinhibitory domain of the Sso t-SNAREs promotes binding of the Vsm1 SNARE regulator in yeast. Mol Biol Cell. 2003;14:3114-25 pubmed
    ..Importantly, Vsm1 binding to Sso seems to preclude Sso binding to its partner t-SNARE, Sec9, and vice versa. This is consistent with the idea that Vsm1 is an inhibitor of SNARE assembly in yeast...
  39. Van Zyl J, Den Haan R, van Zyl W. Over-expression of native Saccharomyces cerevisiae exocytic SNARE genes increased heterologous cellulase secretion. Appl Microbiol Biotechnol. 2014;98:5567-78 pubmed publisher
    ..This work illustrates the potential of engineering components of the anterograde secretory pathway, particularly its SNARE components, for the improvement of heterologous cellulase secretion. ..
  40. Coluccio A, Malzone M, Neiman A. Genetic evidence of a role for membrane lipid composition in the regulation of soluble NEM-sensitive factor receptor function in Saccharomyces cerevisiae. Genetics. 2004;166:89-97 pubmed
    SEC9 and SPO20 encode SNARE proteins related to the mammalian SNAP-25 family. Sec9p associates with the SNAREs Sso1/2p and Snc1/2p to promote the fusion of vesicles with the plasma membrane...
  41. Antebi A, Fink G. The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol Biol Cell. 1992;3:633-54 pubmed
    ..Some of these interactions are modulated by changes in external Ca2+ concentrations. These results imply a global role for Ca2+ in the proper function of components governing transit and processing through the secretory pathway. ..
  42. Katz L, Brennwald P. Testing the 3Q:1R "rule": mutational analysis of the ionic "zero" layer in the yeast exocytic SNARE complex reveals no requirement for arginine. Mol Biol Cell. 2000;11:3849-58 pubmed
  43. Lillie S, Brown S. Smy1p, a kinesin-related protein that does not require microtubules. J Cell Biol. 1998;140:873-83 pubmed
    ..We conclude that Smy1p does not act as a microtubule-based motor to localize properly or to compensate for defective Myo2p, but that it must instead act in some novel way. ..
  44. Williams D, Novick P. Analysis of SEC9 suppression reveals a relationship of SNARE function to cell physiology. PLoS ONE. 2009;4:e5449 pubmed publisher
    ..Second, Sro7p acts to promote SNARE complex formation. Finally, Sec9p function and SNARE complex formation are tightly coupled to the physiological state of the cell.
  45. Ossig R, Schmitt H, de Groot B, Riedel D, Keranen S, Ronne H, et al. Exocytosis requires asymmetry in the central layer of the SNARE complex. EMBO J. 2000;19:6000-10 pubmed
    ..We conclude that the asymmetric arrangement of the polar amino acids in the central layer is essential for normal function of SNAREs in membrane fusion. ..
  46. Neiman A, Katz L, Brennwald P. Identification of domains required for developmentally regulated SNARE function in Saccharomyces cerevisiae. Genetics. 2000;155:1643-55 pubmed
    ..both gene products participate in post-Golgi vesicle fusion events, they cannot substitute for one another; Sec9p is active primarily in vegetative cells while Spo20p functions only during sporulation...
  47. Pelham H. SNAREs and the secretory pathway-lessons from yeast. Exp Cell Res. 1999;247:1-8 pubmed
    ..This review summarizes current knowledge of the yeast SNAREs and the picture of the secretory pathway that emerges from such studies. ..
  48. Rossi G, Brennwald P. Yeast homologues of lethal giant larvae and type V myosin cooperate in the regulation of Rab-dependent vesicle clustering and polarized exocytosis. Mol Biol Cell. 2011;22:842-57 pubmed publisher
    ..Thus Sro7 appears to coordinate the spatial and temporal nature of both Rab-dependent tethering and SNARE-dependent membrane fusion of exocytic vesicles with the plasma membrane. ..
  49. Nair U, Jotwani A, Geng J, Gammoh N, Richerson D, Yen W, et al. SNARE proteins are required for macroautophagy. Cell. 2011;146:290-302 pubmed publisher
    ..Additionally, we found that the endosomal Q/t-SNARE Tlg2 and the R/v-SNAREs Sec22 and Ykt6 interact with Sso1-Sec9, and are required for normal Atg9 transport...
  50. Liu D, Novick P. Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p. J Cell Biol. 2014;207:59-72 pubmed publisher
    ..Similar to Sec3p, the actin-independent localization of Exo70p requires a synergistic interaction with the phosphoinositide PI(4,5)P2. ..
  51. Couve A, Gerst J. Yeast Snc proteins complex with Sec9. Functional interactions between putative SNARE proteins. J Biol Chem. 1994;269:23391-4 pubmed
    ..Here we show that Snc proteins form a tight genetic and physical interaction with a plasma membrane protein, Sec9. Sec9 is the yeast equivalent of SNAP-25, a second receptor protein from neurons that has been shown to interact ..
  52. Dubuke M, Maniatis S, Shaffer S, Munson M. The Exocyst Subunit Sec6 Interacts with Assembled Exocytic SNARE Complexes. J Biol Chem. 2015;290:28245-56 pubmed publisher
    ..previously that the Saccharomyces cerevisiae exocyst subunit Sec6 directly bound the plasma membrane SNARE protein Sec9 in vitro and that Sec6 inhibited the assembly of the binary Sso1-Sec9 SNARE complex...