Gene Symbol: SEC7
Description: Arf family guanine nucleotide exchange factor SEC7
Alias: Arf family guanine nucleotide exchange factor SEC7
Species: Saccharomyces cerevisiae S288c
Products:     SEC7

Top Publications

  1. Franzusoff A, Redding K, Crosby J, Fuller R, Schekman R. Localization of components involved in protein transport and processing through the yeast Golgi apparatus. J Cell Biol. 1991;112:27-37 pubmed
    Saccharomyces cerevisiae sec7 mutants exhibit pleiotropic deficiencies in the transit of proteins through the Golgi apparatus, and elaborate an array of Golgi apparatus-like cisternae at a restrictive growth temperature (37 degrees C)...
  2. Franzusoff A, Lauzé E, Howell K. Immuno-isolation of Sec7p-coated transport vesicles from the yeast secretory pathway. Nature. 1992;355:173-5 pubmed
    ..Mutations in the yeast SEC7 gene disrupt protein transport in the secretory pathway at the nonpermissive temperature...
  3. Jones S, Jedd G, Kahn R, Franzusoff A, Bartolini F, Segev N. Genetic interactions in yeast between Ypt GTPases and Arf guanine nucleotide exchangers. Genetics. 1999;152:1543-56 pubmed
    ..Here we report the identification of a new member of the Sec7-domain family, SYT1, as a high-copy suppressor of a ypt31/32 mutation...
  4. Devasahayam G, Ritz D, Helliwell S, Burke D, Sturgill T. Pmr1, a Golgi Ca2+/Mn2+-ATPase, is a regulator of the target of rapamycin (TOR) signaling pathway in yeast. Proc Natl Acad Sci U S A. 2006;103:17840-5 pubmed
    ..These two phenotypes suggest up-regulation of Npr1 function in the absence of Pmr1. Together, our results establish that Pmr1-dependent Ca2+ and/or Mn2+ ion homeostasis is necessary for TOR signaling. ..
  5. Dechamps C, Portetelle D, Vandenbol M. Multicopy suppression screen in the msb3 msb4 Saccharomyces cerevisiae double mutant, affected in Ypt/RabGAP activity. Biotechnol Lett. 2005;27:1439-49 pubmed
  6. Belgareh Touzé N, Avaro S, Rouille Y, Hoflack B, Haguenauer Tsapis R. Yeast Vps55p, a functional homolog of human obesity receptor gene-related protein, is involved in late endosome to vacuole trafficking. Mol Biol Cell. 2002;13:1694-708 pubmed
    ..Therefore, the function of Vps55p has been conserved throughout evolution. This study highlights the importance of the multispanning Vps55p and OB-RGRP in membrane trafficking to the vacuole/lysosome of eukaryotic cells. ..
  7. Barlowe C. Coupled ER to Golgi transport reconstituted with purified cytosolic proteins. J Cell Biol. 1997;139:1097-108 pubmed
    ..Ordering experiments using the dilution resistant intermediate and reversible Sec23p complex inhibition indicate Sec18p action is required before LMA1 function. ..
  8. van der Vaart A, Griffith J, Reggiori F. Exit from the Golgi is required for the expansion of the autophagosomal phagophore in yeast Saccharomyces cerevisiae. Mol Biol Cell. 2010;21:2270-84 pubmed publisher autophagy and have determined that in yeast, activation of ADP-ribosylation factor (Arf)1 and Arf2 GTPases by Sec7, Gea1, and Gea2 is essential for this catabolic process...
  9. Richardson B, Halaby S, Gustafson M, Fromme J. The Sec7 N-terminal regulatory domains facilitate membrane-proximal activation of the Arf1 GTPase. elife. 2016;5: pubmed publisher
    ..We report a crystal structure revealing that the N-terminal DCB and HUS regulatory domains of the Arf-GEF Sec7 form a single structural unit...

More Information


  1. Stearns T, Willingham M, Botstein D, Kahn R. ADP-ribosylation factor is functionally and physically associated with the Golgi complex. Proc Natl Acad Sci U S A. 1990;87:1238-42 pubmed
    ..Together, these results indicate that ARF functions in intracellular protein transport to or within the Golgi apparatus, a role not predicted by the previous in vitro biochemical studies. ..
  2. Gloor Y, Schöne M, Habermann B, Ercan E, Beck M, Weselek G, et al. Interaction between Sec7p and Pik1p: the first clue for the regulation of a coincidence detection signal. Eur J Cell Biol. 2010;89:575-83 pubmed publisher
  3. Sata M, Donaldson J, Moss J, Vaughan M. Brefeldin A-inhibited guanine nucleotide-exchange activity of Sec7 domain from yeast Sec7 with yeast and mammalian ADP ribosylation factors. Proc Natl Acad Sci U S A. 1998;95:4204-8 pubmed
    The Saccharomyces cerevisiae Sec7 protein (ySec7p), which is an important component of the yeast secretory pathway, contains a sequence of approximately 200 amino acids referred to as a Sec7 domain...
  4. Walch Solimena C, Novick P. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat Cell Biol. 1999;1:523-5 pubmed
  5. Richardson B, McDonold C, Fromme J. The Sec7 Arf-GEF is recruited to the trans-Golgi network by positive feedback. Dev Cell. 2012;22:799-810 pubmed publisher
    ..a detailed kinetic characterization of a functional Golgi Arf-GEF, the trans-Golgi network (TGN)-localized Sec7 protein from yeast. We demonstrate that Sec7 is regulated by both autoinhibition and positive feedback...
  6. Bacon R, Salminen A, Ruohola H, Novick P, Ferro Novick S. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J Cell Biol. 1989;109:1015-22 pubmed
    ..We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus. ..
  7. Dehring D, Adler A, Hosseini A, Hicke L. A C-terminal sequence in the guanine nucleotide exchange factor Sec7 mediates Golgi association and interaction with the Rsp5 ubiquitin ligase. J Biol Chem. 2008;283:34188-96 pubmed publisher
    ..Outside of their conserved catalytic domains, known as Sec7 domains, little is known about Arf GEFs...
  8. Qiu B, Zhang K, Wang S, Sun F. C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation. Biochem Biophys Res Commun. 2014;446:380-6 pubmed publisher
    ..There are six families of Arf-GEFs with a common guanine exchange catalytic domain (Sec7 domain) and various mechanisms of guanine exchange activity regulation...
  9. Deitz S, Rambourg A, Kepes F, Franzusoff A. Sec7p directs the transitions required for yeast Golgi biogenesis. Traffic. 2000;1:172-83 pubmed
    ..The sec7-4 temperature-sensitive allele is a mutation in the highly conserved Sec7 domain (Sec7d) found in all ARF-guanine ..
  10. Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5021-37 pubmed
    ..Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)-Golgi shuttle might be involved in concentrating mRNA at the ER. ..
  11. Tomala K, Korona R. Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones. Mol Genet Genomics. 2008;280:409-17 pubmed publisher
    ..We suggest that under non-stressful conditions, molecular chaperones are tuned to support folding of native proteins, but not that of mutated ones. ..
  12. Wolf J, Nicks M, Deitz S, van Tuinen E, Franzusoff A. An N-end rule destabilization mutant reveals pre-Golgi requirements for Sec7p in yeast membrane traffic. Biochem Biophys Res Commun. 1998;243:191-8 pubmed
    b>Sec7 protein (Sec7p) is required for membrane traffic in the yeast secretory pathway. Because Sec7p regulates more than one stage in the pathway, it has been difficult to assign the most proximal requirement for Sec7p action...
  13. Achstetter T, Franzusoff A, Field C, Schekman R. SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus. J Biol Chem. 1988;263:11711-7 pubmed
    Saccharomyces cerevisiae with mutations at the sec7 locus are pleiotropically deficient in protein transport within the Golgi apparatus and proliferate a large array of Golgi cisternae at a restrictive growth temperature (37 degrees C)...
  14. Basco R, Cueva R, Andaluz E, Larriba G. In vivo processing of the precursor of the major exoglucanase by KEX2 endoprotease in the Saccharomyces cerevisiae secretory pathway. Biochim Biophys Acta. 1996;1310:110-8 pubmed the endoplasmic reticulum but was processed to the mature form (form B) before the block imposed by the sec7 mutation...
  15. McDonold C, Fromme J. Four GTPases differentially regulate the Sec7 Arf-GEF to direct traffic at the trans-golgi network. Dev Cell. 2014;30:759-67 pubmed publisher
    ..The Golgi Arf-GEF Sec7 and the homologous BIG1/2 proteins are effectors of the Arf1 and Arl1 GTPases...