SEC5

Summary

Gene Symbol: SEC5
Description: exocyst subunit SEC5
Alias: exocyst subunit SEC5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Zhang X, Bi E, Novick P, Du L, Kozminski K, Lipschutz J, et al. Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem. 2001;276:46745-50 pubmed
    ..These results have revealed a role for Cdc42 in exocytosis. We propose that Cdc42 coordinates the vesicle docking machinery and the actin cytoskeleton for polarized secretion. ..
  2. Finger F, Novick P. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:647-62 pubmed
    ..The SEC3 gene in high copy suppresses pfy1-111 and sec5-24 and causes synthetic growth defects with ypt1, sec8-9, sec10-2, and sec15-1...
  3. Adamo J, Moskow J, Gladfelter A, Viterbo D, Lew D, Brennwald P. Yeast Cdc42 functions at a late step in exocytosis, specifically during polarized growth of the emerging bud. J Cell Biol. 2001;155:581-92 pubmed
    ..Rather, we suggest that Cdc42 acts as an allosteric regulator of the vesicle docking and fusion apparatus to provide maximal function at sites of polarized growth. ..
  4. Roth D, Guo W, Novick P. Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell. 1998;9:1725-39 pubmed
  5. Wiederkehr A, De Craene J, Ferro Novick S, Novick P. Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol. 2004;167:875-87 pubmed
    ..Unlike the other five exocyst genes, SEC3, SEC5, and EXO70 are not essential for growth or secretion when either the upstream activator rab, Sec4p, or the ..
  6. Hutagalung A, Coleman J, Pypaert M, Novick P. An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components. Mol Biol Cell. 2009;20:153-63 pubmed publisher
    ..The results suggest that either Exo70p or Sec3p must associate with the plasma membrane for the exocyst to function as a vesicle tether. ..
  7. He B, Xi F, Zhang X, Zhang J, Guo W. Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane. EMBO J. 2007;26:4053-65 pubmed
    ..We propose that Exo70 mediates the assembly of the exocyst complex at the plasma membrane, which is a crucial step in the tethering of post-Golgi secretory vesicles for exocytosis. ..
  8. Terbush D, Maurice T, Roth D, Novick P. The Exocyst is a multiprotein complex required for exocytosis in Saccharomyces cerevisiae. EMBO J. 1996;15:6483-94 pubmed
    ..cerevisiae. Mutations in three more of these genes, SEC3, SEC5 and SEC10, were found to disrupt the subunit integrity of the Sec6-Sec8-Sec15 complex, indicating that these genes ..
  9. Shen D, Yuan H, Hutagalung A, Verma A, Kümmel D, Wu X, et al. The synaptobrevin homologue Snc2p recruits the exocyst to secretory vesicles by binding to Sec6p. J Cell Biol. 2013;202:509-26 pubmed publisher
    ..We propose that the exocyst is recruited to secretory vesicles by the combinatorial signals of Sec4-GTP and the Snc proteins. This could help to confer both specificity and directionality to vesicular traffic. ..

More Information

Publications40

  1. Zhang X, Zajac A, Zhang J, Wang P, Li M, Murray J, et al. The critical role of Exo84p in the organization and polarized localization of the exocyst complex. J Biol Chem. 2005;280:20356-64 pubmed
    ..Exo84p plays a critical role in both the assembly of the exocyst and its targeting to sites of secretion. ..
  2. Kozminski K, Alfaro G, Dighe S, Beh C. Homologues of oxysterol-binding proteins affect Cdc42p- and Rho1p-mediated cell polarization in Saccharomyces cerevisiae. Traffic. 2006;7:1224-42 pubmed
    ..Disruption of all OSH gene function caused specific defects in polarized exocytosis, indicating that the Osh proteins are collectively required for a secretory pathway implicated in the maintenance of polarized growth. ..
  3. Guo W, Roth D, Walch Solimena C, Novick P. The exocyst is an effector for Sec4p, targeting secretory vesicles to sites of exocytosis. EMBO J. 1999;18:1071-80 pubmed
    ..Sec4p may control the assembly of the exocyst. The exocyst may therefore function as a rab effector system for targeted secretion. ..
  4. Guo W, Grant A, Novick P. Exo84p is an exocyst protein essential for secretion. J Biol Chem. 1999;274:23558-64 pubmed
    ..Overexpression of Exo84p selectively suppresses the temperature sensitivity of a sec5 mutant...
  5. Finger F, Novick P. Spatial regulation of exocytosis: lessons from yeast. J Cell Biol. 1998;142:609-12 pubmed
  6. Luo G, Zhang J, Luca F, Guo W. Mitotic phosphorylation of Exo84 disrupts exocyst assembly and arrests cell growth. J Cell Biol. 2013;202:97-111 pubmed publisher
    ..Our study demonstrates the coordination between membrane trafficking and cell cycle progression and provides a molecular mechanism by which cell growth is controlled during the cell division cycle. ..
  7. Guo W, Tamanoi F, Novick P. Spatial regulation of the exocyst complex by Rho1 GTPase. Nat Cell Biol. 2001;3:353-60 pubmed
    ..These results reveal the action of parallel pathways for the polarized localization of the exocytic machinery, both of which are under the control of Rho1, a master regulator of cell polarity. ..
  8. Toikkanen J, Miller K, Söderlund H, Jantti J, Keränen S. The beta subunit of the Sec61p endoplasmic reticulum translocon interacts with the exocyst complex in Saccharomyces cerevisiae. J Biol Chem. 2003;278:20946-53 pubmed
    ..These findings propose a novel functional and physical link between the endoplasmic reticulum translocation complex and the exocyst. ..
  9. Novick P, Field C, Schekman R. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell. 1980;21:205-15 pubmed
    ..We suggest that these structures represent intermediates in a pathway in which secretion and plasma membrane assembly are colinear. ..
  10. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  11. Wu H, Turner C, Gardner J, Temple B, Brennwald P. The Exo70 subunit of the exocyst is an effector for both Cdc42 and Rho3 function in polarized exocytosis. Mol Biol Cell. 2010;21:430-42 pubmed publisher
    ..These data suggest that interaction with the Exo70 component of the exocyst is a key event in spatial regulation of exocytosis by Rho GTPases. ..
  12. Govindan B, Bowser R, Novick P. The role of Myo2, a yeast class V myosin, in vesicular transport. J Cell Biol. 1995;128:1055-68 pubmed
    ..Our observations are consistent with a role for Myo2 in transporting a class of secretory vesicles from the mother cell along actin cables into the bud. ..
  13. Zhang X, Wang P, Gangar A, Zhang J, Brennwald P, TerBush D, et al. Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis. J Cell Biol. 2005;170:273-83 pubmed
    ..We propose that, although Lgl is broadly distributed in the cells, its localized interaction with the exocyst and kinetic activation are important for the establishment and reenforcement of cell polarity. ..
  14. Nair J, Muller H, Peterson M, Novick P. Sec2 protein contains a coiled-coil domain essential for vesicular transport and a dispensable carboxy terminal domain. J Cell Biol. 1990;110:1897-909 pubmed
    ..The Sec2 protein may function in conjunction with the Sec4 and Sec15 proteins to control vesicular traffic. ..
  15. Walch Solimena C, Novick P. The yeast phosphatidylinositol-4-OH kinase pik1 regulates secretion at the Golgi. Nat Cell Biol. 1999;1:523-5 pubmed
  16. Aalto M, Jantti J, Ostling J, Keranen S, Ronne H. Mso1p: a yeast protein that functions in secretion and interacts physically and genetically with Sec1p. Proc Natl Acad Sci U S A. 1997;94:7331-6 pubmed
    ..These findings suggest that Mso1p is a component of the secretory vesicle docking complex whose function is closely associated with that of Sec1p. ..
  17. Jin Y, Sultana A, Gandhi P, Franklin E, Hamamoto S, Khan A, et al. Myosin V transports secretory vesicles via a Rab GTPase cascade and interaction with the exocyst complex. Dev Cell. 2011;21:1156-70 pubmed publisher
    ..Moreover, these studies predict that for many pathways, molecular motors attach to vesicles prior to their formation and remain attached until fusion...
  18. Grosshans B, Andreeva A, Gangar A, Niessen S, Yates J, Brennwald P, et al. The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. J Cell Biol. 2006;172:55-66 pubmed
    ..Genetic data support our conclusion that Sro7p functions downstream of Sec4p and further imply that Sro7p and the exocyst share partially overlapping functions, possibly in SNARE regulation. ..
  19. Orlando K, Sun X, Zhang J, Lu T, Yokomizo L, Wang P, et al. Exo-endocytic trafficking and the septin-based diffusion barrier are required for the maintenance of Cdc42p polarization during budding yeast asymmetric growth. Mol Biol Cell. 2011;22:624-33 pubmed publisher
    ..Collectively, membrane trafficking and septins function synergistically to maintain the dynamic polarization of Cdc42p during asymmetric growth in yeast. ..
  20. Liu D, Novick P. Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p. J Cell Biol. 2014;207:59-72 pubmed publisher
    ..Similar to Sec3p, the actin-independent localization of Exo70p requires a synergistic interaction with the phosphoinositide PI(4,5)P2. ..
  21. Tomeo M, Palermo L, Tove S, Parks L. A conditional sterol esterification defect in yeast having either a sec1 or sec5 mutation in the secretory pathway. Yeast. 1997;13:449-62 pubmed
    Two temperature-conditional secretory mutations, sec1 and sec5, cause the accumulation of post-Golgi vesicles when strains containing these mutations are grown at 37 degrees C...
  22. Medkova M, France Y, Coleman J, Novick P. The rab exchange factor Sec2p reversibly associates with the exocyst. Mol Biol Cell. 2006;17:2757-69 pubmed
    ..The mislocalization of Sec2p mutants results from a failure to be released from Sec15p, blocking this recycling pathway. ..
  23. Aalto M, Ronne H, Keranen S. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J. 1993;12:4095-104 pubmed
    ..copy number can suppress sec1 mutations and also mutations in several other late acting SEC genes, such as SEC3, SEC5, SEC9 and SEC15...
  24. Terbush D, Guo W, Dunkelbarger S, Novick P. Purification and characterization of yeast exocyst complex. Methods Enzymol. 2001;329:100-10 pubmed
  25. Luo G, Zhang J, Guo W. The role of Sec3p in secretory vesicle targeting and exocyst complex assembly. Mol Biol Cell. 2014;25:3813-22 pubmed publisher
    ..Our study helps to establish the role of the exocyst subunits in tethering and allows the investigation of the mechanisms that regulate vesicle tethering during exocytosis. ..
  26. France Y, Boyd C, Coleman J, Novick P. The polarity-establishment component Bem1p interacts with the exocyst complex through the Sec15p subunit. J Cell Sci. 2006;119:876-88 pubmed
    ..This, in turn, helps to coordinate the secretory pathway and polarized bud growth. ..
  27. Songer J, Munson M. Sec6p anchors the assembled exocyst complex at sites of secretion. Mol Biol Cell. 2009;20:973-82 pubmed publisher
    ..Our results indicate that assembly and polarization of the exocyst are functionally separable events, and that Sec6p is required to anchor exocyst complexes at sites of secretion. ..
  28. Novick P, Ferro S, Schekman R. Order of events in the yeast secretory pathway. Cell. 1981;25:461-9 pubmed
  29. Stalder D, Novick P. The casein kinases Yck1p and Yck2p act in the secretory pathway, in part, by regulating the Rab exchange factor Sec2p. Mol Biol Cell. 2016;27:686-701 pubmed publisher
    ..This promotes Sec2p function by stimulating its interaction with Sec15p. Finally, Sec2p is dephosphorylated very late in the exocytic reaction to facilitate recycling. ..
  30. Prigent M, Boy Marcotte E, Chesneau L, Gibson K, Dupre Crochet S, Tisserand H, et al. The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic. 2011;12:1084-97 pubmed publisher
  31. Lillie S, Brown S. Smy1p, a kinesin-related protein that does not require microtubules. J Cell Biol. 1998;140:873-83 pubmed
    ..We conclude that Smy1p does not act as a microtubule-based motor to localize properly or to compensate for defective Myo2p, but that it must instead act in some novel way. ..