Gene Symbol: SEC27
Description: coatomer subunit beta'
Alias: coatomer subunit beta'
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Eugster A, Frigerio G, Dale M, Duden R. The alpha- and beta'-COP WD40 domains mediate cargo-selective interactions with distinct di-lysine motifs. Mol Biol Cell. 2004;15:1011-23 pubmed
    ..The protein is degraded more rapidly in cells with a point mutation in the WD40 domain of beta'-COP (sec27-95) or in cells lacking the domain altogether, whereas a point mutation in the Clathrin Heavy Chain Repeat (sec27-..
  2. Eugster A, Frigerio G, Dale M, Duden R. COP I domains required for coatomer integrity, and novel interactions with ARF and ARF-GAP. EMBO J. 2000;19:3905-17 pubmed
    ..Glo3p also interacts with intact coatomer in vitro. ..
  3. Letourneur F, Gaynor E, Hennecke S, Démollière C, Duden R, Emr S, et al. Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum. Cell. 1994;79:1199-207 pubmed
    ..sec22, and sec23), a defect in retrieval was observed in previously characterized coatomer mutants (sec21-1, sec27-1), as well as in newly isolated retrieval mutants (sec21-2, ret1-1)...
  4. Duden R, Hosobuchi M, Hamamoto S, Winey M, Byers B, Schekman R. Yeast beta- and beta'-coat proteins (COP). Two coatomer subunits essential for endoplasmic reticulum-to-Golgi protein traffic. J Biol Chem. 1994;269:24486-95 pubmed
    ..traffic, we have cloned and characterized two essential genes encoding subunits of the yeast coatomer, SEC26 and SEC27. Sec26p is a 109-kDa protein that shares 43% sequence identity with mammalian beta-coat protein (beta-COP)...
  5. Gabriely G, Kama R, Gerst J. Involvement of specific COPI subunits in protein sorting from the late endosome to the vacuole in yeast. Mol Cell Biol. 2007;27:526-40 pubmed
    ..Y (CPY) was partially missorted to the cell surface in certain mutants of the COPIB subcomplex (COPIb; Sec27, Sec28, and possibly Sec33), which indicates an impairment in endosomal transport...
  6. Spang A, Herrmann J, Hamamoto S, Schekman R. The ADP ribosylation factor-nucleotide exchange factors Gea1p and Gea2p have overlapping, but not redundant functions in retrograde transport from the Golgi to the endoplasmic reticulum. Mol Biol Cell. 2001;12:1035-45 pubmed
    ..In contrast, Sec7p, which is required for protein transport within the Golgi, is not required for retrograde protein trafficking. ..
  7. Luo Z, Gallwitz D. Biochemical and genetic evidence for the involvement of yeast Ypt6-GTPase in protein retrieval to different Golgi compartments. J Biol Chem. 2003;278:791-9 pubmed
    ..The results obtained indicate that Ypt6p acts in endosome-to-Golgi, in intra-Golgi retrograde transport, and possibly also in Golgi-to-ER trafficking. ..
  8. Jarmoszewicz K, Łukasiak K, Riezman H, Kaminska J. Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants. PLoS ONE. 2012;7:e39582 pubmed publisher
    ..The importance of this modification of COPI proteins is unknown. With the exception of the Sec27 protein (?'COP) neither the ubiquitin ligase responsible for ubiquitination of COPI subunits nor the importance of ..
  9. Slobodin B, Gerst J. A novel mRNA affinity purification technique for the identification of interacting proteins and transcripts in ribonucleoprotein complexes. RNA. 2010;16:2277-90 pubmed publisher
    ..By isolating tagged OXA1 mRNA using RaPID, we could identify a yeast COPI subunit (i.e., Sec27) as a candidate interacting protein...

More Information


  1. Peyroche A, Courbeyrette R, Rambourg A, Jackson C. The ARF exchange factors Gea1p and Gea2p regulate Golgi structure and function in yeast. J Cell Sci. 2001;114:2241-53 pubmed
    ..In addition, the structure of Golgi elements was severely perturbed in gea mutants. We conclude that Gea1p and Gea2p play an important role in the structure and functioning of the Golgi apparatus in yeast. ..
  2. Eremenko E, Ben Zvi A, Morozova Roche L, Raveh D. Aggregation of human S100A8 and S100A9 amyloidogenic proteins perturbs proteostasis in a yeast model. PLoS ONE. 2013;8:e58218 pubmed publisher
    ..on a background of unrelated metastable temperature-sensitive mutant proteins, Cdc53-1p, Cdc34-2p, Srp1-31p and Sec27-1p...
  3. Yu X, Breitman M, Goldberg J. A structure-based mechanism for Arf1-dependent recruitment of coatomer to membranes. Cell. 2012;148:530-42 pubmed publisher
    ..A bivalent GTP-dependent binding mode has implications for the dynamics of coatomer interaction with the Golgi and for the selection of cargo molecules. ..
  4. Lee R, Brunette S, Puente L, Megeney L. Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A. 2010;107:13348-53 pubmed publisher
    ..Together, our results show that Yca1 contributes to the fitness and adaptability of growing yeast through an aggregate remodeling activity. ..
  5. Trautwein M, Dengjel J, Schirle M, Spang A. Arf1p provides an unexpected link between COPI vesicles and mRNA in Saccharomyces cerevisiae. Mol Biol Cell. 2004;15:5021-37 pubmed
    ..Hereby acts the SHE machinery in long-range mRNA transport, whereas COPI vesicles could act as short-range and localization vehicles. The endoplasmic reticulum (ER)-Golgi shuttle might be involved in concentrating mRNA at the ER. ..
  6. Hirata R, Nihei C, Nakano A. Isoform-selective oligomer formation of Saccharomyces cerevisiae p24 family proteins. J Biol Chem. 2013;288:37057-70 pubmed publisher
    ..This complex was mainly localized to the Golgi, whereas the p24 complex containing Erv25, instead of Rrt6 but otherwise with the same isoform composition, was found mostly in the ER...
  7. Esposito A, Kinzy T. The eukaryotic translation elongation Factor 1Bgamma has a non-guanine nucleotide exchange factor role in protein metabolism. J Biol Chem. 2010;285:37995-8004 pubmed publisher
    ..Finally, eEF1B? co-immunoprecipitates with an essential component of ER-Golgi transport vesicles. Taken together, these results support a broader protein metabolism role for eEF1B?. ..
  8. Xu P, Hankins H, MacDonald C, Erlinger S, Frazier M, Diab N, et al. COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal. elife. 2017;6: pubmed publisher
  9. Tu L, Tai W, Chen L, Banfield D. Signal-mediated dynamic retention of glycosyltransferases in the Golgi. Science. 2008;321:404-7 pubmed publisher
    ..We propose that Vps74p maintains the steady-state localization of Golgi glycosyltransferases dynamically, by promoting their incorporation into COPI-coated vesicles. ..
  10. Yip C, Walz T. Molecular structure and flexibility of the yeast coatomer as revealed by electron microscopy. J Mol Biol. 2011;408:825-31 pubmed publisher
    ..Our analyses provide the first three-dimensional picture of the complete coatomer and reveal substantial conformational flexibility likely to be critical for its scaffolding function. ..
  11. Sato M, Sato K, Nakano A. Evidence for the intimate relationship between vesicle budding from the ER and the unfolded protein response. Biochem Biophys Res Commun. 2002;296:560-7 pubmed
    ..The overexpression of IRE1 suppresses the sec mutants defective in vesicle budding from the ER but not others, highlighting a close relationship between the ER exit and the UPR. ..
  12. Rein U, Andag U, Duden R, Schmitt H, Spang A. ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat. J Cell Biol. 2002;157:395-404 pubmed
    ..The mechanisms by which v-SNAREs interact with COPI and COPII coat proteins seem to be different and may play a key role in determining specificity in vesicle budding...
  13. Kim D, Massey T, Sacher M, Pypaert M, Ferro Novick S. Sgf1p, a new component of the Sec34p/Sec35p complex. Traffic. 2001;2:820-30 pubmed
    ..Although an earlier study suggested that Sec34p (Grd20p) is not required for protein secretion, we show here that the sec34-2 and sec35-1 mutations lead to a pleiotropic block in the secretion of all proteins into the growth medium. ..
  14. Frigerio G. The Saccharomyces cerevisiae early secretion mutant tip20 is synthetic lethal with mutants in yeast coatomer and the SNARE proteins Sec22p and Ufe1p. Yeast. 1998;14:633-46 pubmed
    ..A subset of tip20 mutants was found to be lethal in combination with sec20-1, sec21-1, sec22-3 and sec27-1...
  15. Michelsen K, Schmid V, Metz J, Heusser K, Liebel U, Schwede T, et al. Novel cargo-binding site in the beta and delta subunits of coatomer. J Cell Biol. 2007;179:209-17 pubmed
    ..A homology model of the COPI trunk domain illustrates the recognition of R-based signals by COPI. ..
  16. Kim K, Kim E, Jeong K, Park Y, Park H. Effects of mutations in the WD40 domain of ?-COP on its interaction with the COPI coatomer in Saccharomyces cerevisiae. J Microbiol. 2012;50:256-62 pubmed publisher
    ..Furthermore, although the WD40 domain is dispensable for interaction of ?-COP with ?-COP, structural alterations in the WD40 domain could impair the interaction. ..