Genomes and Genes
Gene Symbol: SEC14
Description: phosphatidylinositol/phosphatidylcholine transfer protein SEC14
Alias: PIT1, phosphatidylinositol/phosphatidylcholine transfer protein SEC14
Species: Saccharomyces cerevisiae S288c
- Skinner H, McGee T, McMaster C, Fry M, Bell R, Bankaitis V. The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity. Proc Natl Acad Sci U S A. 1995;92:112-6 pubmed
- Huijbregts R, Topalof L, Bankaitis V. Lipid metabolism and regulation of membrane trafficking. Traffic. 2000;1:195-202 pubmed..The 1990s, and particularly its last half, has brought fundamental reappraisals of the interface of lipids and lipid metabolism in regulating intracellular membrane trafficking events. Some of the emerging themes are reviewed here. ..
- Howe A, Fairn G, Macdonald K, Bankaitis V, McMaster C. Regulation of phosphoinositide levels by the phospholipid transfer protein Sec14p controls Cdc42p/p21-activated kinase-mediated cell cycle progression at cytokinesis. Eukaryot Cell. 2007;6:1814-23 pubmed..In this study, Saccharomyces cerevisiae cells containing a SEC14 temperature-sensitive allele along with an inactivated CDP-choline pathway were transformed with a high-copy-..
- Patton Vogt J, Griac P, Sreenivas A, Bruno V, Dowd S, Swede M, et al. Role of the yeast phosphatidylinositol/phosphatidylcholine transfer protein (Sec14p) in phosphatidylcholine turnover and INO1 regulation. J Biol Chem. 1997;272:20873-83 pubmed..mutations in the CDP-choline pathway for phosphatidylcholine biosynthesis permit the cell to grow even when the SEC14 gene is completely deleted (Cleves, A., McGee, T., Whitters, E., Champion, K., Aitken, J., Dowhan, W., Goebl, M...
- Xie Z, Fang M, Rivas M, Faulkner A, Sternweis P, Engebrecht J, et al. Phospholipase D activity is required for suppression of yeast phosphatidylinositol transfer protein defects. Proc Natl Acad Sci U S A. 1998;95:12346-51 pubmed..These data suggest that PLD supports 'bypass Sec14p' by generating a phosphatidic acid pool that is somehow utilized in supporting yeast Golgi secretory function. ..
- Rivas M, Kearns B, Xie Z, Guo S, Sekar M, Hosaka K, et al. Pleiotropic alterations in lipid metabolism in yeast sac1 mutants: relationship to "bypass Sec14p" and inositol auxotrophy. Mol Biol Cell. 1999;10:2235-50 pubmed..Finally, we determine that CDP-choline pathway activity contributes to the inositol auxotrophy of sac1 strains in a novel manner that does not involve obvious defects in transcriptional expression of the INO1 gene. ..
- Aitken J, van Heusden G, Temkin M, Dowhan W. The gene encoding the phosphatidylinositol transfer protein is essential for cell growth. J Biol Chem. 1990;265:4711-7 pubmed..of the PI-TP from Saccharomyces cerevisiae was used to screen a yeast genomic library for the gene encoding PI-TP (PIT1 gene)...
- Li X, Rivas M, Fang M, Marchena J, Mehrotra B, Chaudhary A, et al. Analysis of oxysterol binding protein homologue Kes1p function in regulation of Sec14p-dependent protein transport from the yeast Golgi complex. J Cell Biol. 2002;157:63-77 pubmed
- Beh C, Cool L, Phillips J, Rine J. Overlapping functions of the yeast oxysterol-binding protein homologues. Genetics. 2001;157:1117-40 pubmed..Our findings indicated that OSH genes performed a multitude of nonessential roles defined by specific subsets of the genes and that most shared at least one essential role potentially linked to changes in sterol lipid levels. ..
- Phillips S, Sha B, Topalof L, Xie Z, Alb J, Klenchin V, et al. Yeast Sec14p deficient in phosphatidylinositol transfer activity is functional in vivo. Mol Cell. 1999;4:187-97 pubmed..However, its expression efficiently rescues the lethality and Golgi secretory defects associated with sec14-1ts and sec14 null mutations...
- Jones S, Alb J, Phillips S, Bankaitis V, Howell K. A phosphatidylinositol 3-kinase and phosphatidylinositol transfer protein act synergistically in formation of constitutive transport vesicles from the trans-Golgi network. J Biol Chem. 1998;273:10349-54 pubmed..These collective findings indicate that the PI 3-kinase and PITP synergize to form a pool of PI 3-phosphate that is essential for formation of exocytic vesicles from the hepatocyte TGN. ..
- Bankaitis V, Malehorn D, Emr S, Greene R. The Saccharomyces cerevisiae SEC14 gene encodes a cytosolic factor that is required for transport of secretory proteins from the yeast Golgi complex. J Cell Biol. 1989;108:1271-81 pubmedWe have obtained and characterized a genomic clone of SEC14, a Saccharomyces cerevisiae gene whose product is required for export of yeast secretory proteins from the Golgi complex...
- Alfaro G, Johansen J, Dighe S, Duamel G, Kozminski K, Beh C. The sterol-binding protein Kes1/Osh4p is a regulator of polarized exocytosis. Traffic. 2011;12:1521-36 pubmed publisher..These results support a model in which Osh4p acts as a sterol-dependent regulator of polarized vesicle transport, as opposed to being a sterol-transfer protein. ..
- Tripathi A, Nile A, Bankaitis V. Sec14-like phosphatidylinositol-transfer proteins and diversification of phosphoinositide signalling outcomes. Biochem Soc Trans. 2014;42:1383-8 pubmed publisher..In the present paper, we discuss our recent work with Sec14-like PITPs and describe how PITPs integrate diverse territories of the lipid metabolome with phosphoinositide ..
- Tschopp J, Esmon P, Schekman R. Defective plasma membrane assembly in yeast secretory mutants. J Bacteriol. 1984;160:966-70 pubmed..Mutants blocked in transport from the endoplasmic reticulum (sec18), from the Golgi body (sec7 and sec14), and in transport of secretory vesicles (sec1) show dramatically reduced assembly of galactose and arginine ..
- Bacon R, Salminen A, Ruohola H, Novick P, Ferro Novick S. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J Cell Biol. 1989;109:1015-22 pubmed..We have also established genetic interactions between ypt1 and a subset of the other genes required for transport to and through the Golgi apparatus. ..
- Chang H, Jesch S, Gaspar M, Henry S. Role of the unfolded protein response pathway in secretory stress and regulation of INO1 expression in Saccharomyces cerevisiae. Genetics. 2004;168:1899-913 pubmed..Indeed, many of the Sec(-) mutants that had elevated UPR expression at semipermissive growth temperatures failed to achieve wild-type levels of INO1 expression under these same conditions. ..
- Regnacq M, Ferreira T, Puard J, Berges T. SUT1 suppresses sec14-1 through upregulation of CSR1 in Saccharomyces cerevisiae. FEMS Microbiol Lett. 2002;216:165-70 pubmedSUT1 constitutive expression in aerobiosis suppressed the ts phenotype of the sec14-1 mutation, restored growth of the sec14-null mutant and corrected the translocation defect of the vacuolar carboxypeptidase Y...
- Lauwers E, Jacob C, Andre B. K63-linked ubiquitin chains as a specific signal for protein sorting into the multivesicular body pathway. J Cell Biol. 2009;185:493-502 pubmed publisher..Our data reveal that K63-linked Ub chains act as a specific signal for MVB sorting, providing further insight into the Ub code of membrane protein trafficking. ..
- Mousley C, Tyeryar K, Ryan M, Bankaitis V. Sec14p-like proteins regulate phosphoinositide homoeostasis and intracellular protein and lipid trafficking in yeast. Biochem Soc Trans. 2006;34:346-50 pubmed..termed SFH (Sec Fourteen Homologue) proteins, and overexpression of certain individual SFH gene products rescues sec14-1(ts)-associated growth and secretory defects...
- Wong T, Fairn G, Poon P, Shmulevitz M, McMaster C, Singer R, et al. Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Proc Natl Acad Sci U S A. 2005;102:12777-82 pubmed..This effect was not seen for the SEC14 gene, encoding the founding member of the yeast PITP protein family, even though the Gcs1 and Age2 ArfGAPs are ..
- Kagiwada S, Kearns B, McGee T, Fang M, Hosaka K, Bankaitis V. The yeast BSD2-1 mutation influences both the requirement for phosphatidylinositol transfer protein function and derepression of phospholipid biosynthetic gene expression in yeast. Genetics. 1996;143:685-97 pubmed
- Tomeo M, Palermo L, Tove S, Parks L. A conditional sterol esterification defect in yeast having either a sec1 or sec5 mutation in the secretory pathway. Yeast. 1997;13:449-62 pubmed..Double mutants containing sec6, sec14 or sec18 with either a sec1 or sec5 mutation have normal esterification levels...
- Rudge S, Sciorra V, Iwamoto M, Zhou C, Strahl T, Morris A, et al. Roles of phosphoinositides and of Spo14p (phospholipase D)-generated phosphatidic acid during yeast sporulation. Mol Biol Cell. 2004;15:207-18 pubmed..Overexpression of Pik1p, a PtdIns 4-kinase, suppressed the sec14-1 meiosis and spore formation defects; conversely, pik1-ts diploids failed to undergo meiosis and spore formation...
- Simon J, Morimoto T, Bankaitis V, Gottlieb T, Ivanov I, Adesnik M, et al. An essential role for the phosphatidylinositol transfer protein in the scission of coatomer-coated vesicles from the trans-Golgi network. Proc Natl Acad Sci U S A. 1998;95:11181-6 pubmed
- Stevens T, Esmon B, Schekman R. Early stages in the yeast secretory pathway are required for transport of carboxypeptidase Y to the vacuole. Cell. 1982;30:439-48 pubmed..The Golgi body represents a branch point in the pathway: from this organelle, vacuolar proenzymes are transported to the vacuole for proteolytic processing and secretory proteins are packaged into vesicles. ..
- Bankaitis V, Phillips S, Yanagisawa L, Li X, Routt S, Xie Z. Phosphatidylinositol transfer protein function in the yeast Saccharomyces cerevisiae. Adv Enzyme Regul. 2005;45:155-70 pubmed
- Yanagisawa L, Marchena J, Xie Z, Li X, Poon P, Singer R, et al. Activity of specific lipid-regulated ADP ribosylation factor-GTPase-activating proteins is required for Sec14p-dependent Golgi secretory function in yeast. Mol Biol Cell. 2002;13:2193-206 pubmed
- Smirnova T, Chadwick T, MacArthur R, Poluektov O, Song L, Ryan M, et al. The chemistry of phospholipid binding by the Saccharomyces cerevisiae phosphatidylinositol transfer protein Sec14p as determined by EPR spectroscopy. J Biol Chem. 2006;281:34897-908 pubmed..The data further suggest that the Sec14p phospholipid binding pocket provides a polarity gradient that we propose is a primary thermodynamic factor that powers the ability of Sec14p to abstract a phospholipid from a membrane bilayer. ..
- Curwin A, LeBlanc M, Fairn G, McMaster C. Localization of lipid raft proteins to the plasma membrane is a major function of the phospholipid transfer protein Sec14. PLoS ONE. 2013;8:e55388 pubmed publisherThe Sec14 protein domain is a conserved tertiary structure that binds hydrophobic ligands. The Sec14 protein from Saccharomyces cerevisiae is essential with studies of S...
- Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
- Tomala K, Korona R. Alleviation of deleterious effects of protein mutation through inactivation of molecular chaperones. Mol Genet Genomics. 2008;280:409-17 pubmed publisher..We suggest that under non-stressful conditions, molecular chaperones are tuned to support folding of native proteins, but not that of mutated ones. ..
- Murray J, McMaster C. Nte1p-mediated deacylation of phosphatidylcholine functionally interacts with Sec14p. J Biol Chem. 2005;280:8544-52 pubmed..through in vivo PC turnover measurements and observed that intracellular GPCho accumulation is decreased in a sec14(ts) strain shifted to 37 degrees C in 10 mm choline (Cho)-containing medium compared with a Sec14p-proficient ..
- Rudge S, Zhou C, Engebrecht J. Differential regulation of Saccharomyces cerevisiae phospholipase D in sporulation and Sec14-independent secretion. Genetics. 2002;160:1353-61 pubmed..is also required for secretion in the absence of the phosphatidylinositol/phosphatidylcholine transfer protein Sec14 (i.e., Sec14-independent secretion). In sporulating cells Spo14 is phosphorylated and relocalized within the cell...
- Soper J, Kehm V, Burd C, Bankaitis V, Lee V. Aggregation of ?-synuclein in S. cerevisiae is associated with defects in endosomal trafficking and phospholipid biosynthesis. J Mol Neurosci. 2011;43:391-405 pubmed publisher..These data suggest new pathogenic mechanisms for ?-synuclein neurotoxicity. ..
- Mousley C, Davison J, Bankaitis V. Sec14 like PITPs couple lipid metabolism with phosphoinositide synthesis to regulate Golgi functionality. Subcell Biochem. 2012;59:271-87 pubmed publisher..Importantly, Sec14 and Sec14-like proteins are a unique superfamily of proteins that sense specific aspects of lipid metabolism, ..
- Mousley C, Yuan P, Gaur N, Trettin K, Nile A, Deminoff S, et al. A sterol-binding protein integrates endosomal lipid metabolism with TOR signaling and nitrogen sensing. Cell. 2012;148:702-15 pubmed publisher..These data describe a pathway by which Kes1 integrates lipid metabolism with TORC1 signaling and nitrogen sensing. ..
- Ren J, Schaaf G, Bankaitis V, Ortlund E, Pathak M. Crystallization and preliminary X-ray diffraction analysis of Sfh3, a member of the Sec14 protein superfamily. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011;67:1239-43 pubmed publisherb>Sec14 is the major phosphatidylinositol (PtdIns)/phosphatidylcholine (PtdCho) transfer protein in the yeast Saccharomyces cerevisiae and is the founding member of the Sec14 protein superfamily...
- Yamamoto T, Fujimura Kamada K, Shioji E, Suzuki R, Tanaka K. Cfs1p, a Novel Membrane Protein in the PQ-Loop Family, Is Involved in Phospholipid Flippase Functions in Yeast. G3 (Bethesda). 2017;7:179-192 pubmed publisher..Cfs1p may thus be a novel regulator of phospholipid asymmetry. ..
- Demmel L, Beck M, Klose C, Schlaitz A, Gloor Y, Hsu P, et al. Nucleocytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Mol Biol Cell. 2008;19:1046-61 pubmed publisher..These data suggest a role of Pik1p nucleocytoplasmic shuttling in coordination of biosynthetic transport from the Golgi with nutrient signaling. ..
- Griac P. Sec14 related proteins in yeast. Biochim Biophys Acta. 2007;1771:737-45 pubmed..It is a diverse group of proteins with distinct subcellular localizations and varied physiological functions related to lipid metabolism, phosphoinositide mediated signaling and membrane trafficking. ..
- Holic R, Zágorsek M, Griac P. Regulation of phospholipid biosynthesis by phosphatidylinositol transfer protein Sec14p and its homologues. A critical role for phosphatidic acid. Eur J Biochem. 2004;271:4401-8 pubmed..the conditions of increased phosphatidylcholine (PtdCho) turnover, as occurs in the sec14Delta cki1Delta strain (SEC14 encodes the major yeast phosphatidylinositol transfer protein; CKI1 encodes choline kinase of the cytidine ..
- Kearns B, McGee T, Mayinger P, Gedvilaite A, Phillips S, Kagiwada S, et al. Essential role for diacylglycerol in protein transport from the yeast Golgi complex. Nature. 1997;387:101-5 pubmed..The essential function of Sec14p may therefore be to maintain a sufficient pool of DAG in the Golgi to support the production of secretory vesicles. ..
- Boyd A, Ciufo L, Barclay J, Graham M, Haynes L, Doherty M, et al. A random mutagenesis approach to isolate dominant-negative yeast sec1 mutants reveals a functional role for domain 3a in yeast and mammalian Sec1/Munc18 proteins. Genetics. 2008;180:165-78 pubmed publisher..Furthermore, this approach of screening for dominant-negative mutants in yeast may be useful for other conserved proteins, to identify functionally important domains in their mammalian homologs. ..
- Yuan Y, Zhao W, Wang X, Gao Y, Niu L, Teng M. Dimeric Sfh3 has structural changes in its binding pocket that are associated with a dimer-monomer state transformation induced by substrate binding. Acta Crystallogr D Biol Crystallogr. 2013;69:313-23 pubmed publisher..A family of PtdIns-transfer proteins (PITPs) called the Sec14 family have been predicted to form a set of functional modules that can sense different types of lipid metabolism ..
- Ren J, Pei Chen Lin C, Pathak M, Temple B, Nile A, Mousley C, et al. A phosphatidylinositol transfer protein integrates phosphoinositide signaling with lipid droplet metabolism to regulate a developmental program of nutrient stress-induced membrane biogenesis. Mol Biol Cell. 2014;25:712-27 pubmed publisher..Here we demonstrate that yeast Sfh3, an unusual Sec14-like phosphatidylinositol transfer protein, is an LD-associated protein that inhibits lipid mobilization from ..
- Liu H, Bretscher A. Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport. J Cell Biol. 1992;118:285-99 pubmed..We conclude that tropomyosin, and by inference actin cables, may facilitate directed vesicular transport of components to the correct location on the cell surface. ..