Gene Symbol: SEC13
Description: GTPase-activating protein SEC13
Alias: ANU3, GTPase-activating protein SEC13
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Supek F, Madden D, Hamamoto S, Orci L, Schekman R. Sec16p potentiates the action of COPII proteins to bud transport vesicles. J Cell Biol. 2002;158:1029-38 pubmed
    ..We propose that Sec16p nucleates a Sar1-GTP-dependent initiation of COPII assembly and serves to stabilize the coat to premature disassembly after Sar1p hydrolyzes GTP...
  2. Antonny B, Gounon P, Schekman R, Orci L. Self-assembly of minimal COPII cages. EMBO Rep. 2003;4:419-24 pubmed
    The small G-protein Sar1 and the cytosolic complexes Sec23/24 and Sec13/31 associate sequentially on endoplasmic reticulum membranes to form a protein coat named COPII, which drives the formation of transport vesicles...
  3. Antonny B, Madden D, Hamamoto S, Orci L, Schekman R. Dynamics of the COPII coat with GTP and stable analogues. Nat Cell Biol. 2001;3:531-7 pubmed
    ..The two large COPII complexes Sec23/24p and Sec13/31p bind almost instantaneously (in less than 1 s) to Sar1pGTP-doped liposomes...
  4. Alber F, Dokudovskaya S, Veenhoff L, Zhang W, Kipper J, Devos D, et al. The molecular architecture of the nuclear pore complex. Nature. 2007;450:695-701 pubmed
    ..These findings provide clues to the evolutionary origins of the NPC. ..
  5. Siniossoglou S, Wimmer C, Rieger M, Doye V, Tekotte H, Weise C, et al. A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores. Cell. 1996;84:265-75 pubmed
    ..Thus, the Nup84p complex in conjunction with Sec13-type proteins is required for correct nuclear pore biogenesis.
  6. Roberg K, Rowley N, Kaiser C. Physiological regulation of membrane protein sorting late in the secretory pathway of Saccharomyces cerevisiae. J Cell Biol. 1997;137:1469-82 pubmed
    ..We have also found that sorting of Gap1p in the Golgi is controlled by SEC13, a gene previously shown to encode a component of the COPII vesicle coat...
  7. Brohawn S, Schwartz T. Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice. Nat Struct Mol Biol. 2009;16:1173-7 pubmed publisher
    ..Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84-Nup145C-Sec13 of the Y complex...
  8. Debler E, Ma Y, Seo H, Hsia K, Noriega T, Blobel G, et al. A fence-like coat for the nuclear pore membrane. Mol Cell. 2008;32:815-26 pubmed publisher
    ..The Seh1*Nup85 assembly bears resemblance in its shape and dimensions to that of another nucleoporin pair, Sec13*Nup145C...
  9. Lutzmann M, Kunze R, Buerer A, Aebi U, Hurt E. Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins. EMBO J. 2002;21:387-97 pubmed
    ..Taken together, our data document that the Nup84p-Nup133p complex self-assembles in a modular concept from distinct smaller nucleoporin construction sets. ..

More Information


  1. Matsuoka K, Schekman R. The use of liposomes to study COPII- and COPI-coated vesicle formation and membrane protein sorting. Methods. 2000;20:417-28 pubmed
    ..Therefore, both generation of coated vesicles and protein sorting into the vesicles can be reproduced with liposomes and purified proteins. ..
  2. Springer S, Schekman R. Nucleation of COPII vesicular coat complex by endoplasmic reticulum to Golgi vesicle SNAREs. Science. 1998;281:698-700 pubmed
    ..The data suggest that transmembrane proteins can be taken up into COPII vesicles by direct interactions with the coat proteins and may play a structural role in the assembly of the COPII coat complex...
  3. Gimeno R, Espenshade P, Kaiser C. SED4 encodes a yeast endoplasmic reticulum protein that binds Sec16p and participates in vesicle formation. J Cell Biol. 1995;131:325-38 pubmed
    ..Extensive genetic interactions between SAR1, SED4, and SEC16 show close functional links between these proteins and imply that they might function together as a multisubunit complex on the ER membrane. ..
  4. Dokudovskaya S, Waharte F, Schlessinger A, Pieper U, Devos D, Cristea I, et al. A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae. Mol Cell Proteomics. 2011;10:M110.006478 pubmed publisher
    ..Here, we identify the SEA (Seh1-associated) protein complex in yeast that contains the nucleoporin Seh1 and Sec13, the latter subunit of both the nuclear pore complex and the COPII coating complex...
  5. Shaywitz D, Espenshade P, Gimeno R, Kaiser C. COPII subunit interactions in the assembly of the vesicle coat. J Biol Chem. 1997;272:25413-6 pubmed
    ..We propose that Sec16p organizes the assembly of a coat that is stabilized both by the interaction of Sec31p with Sec23p and Sec24p and by the interaction of these three components with Sec16p...
  6. Roberg K, Bickel S, Rowley N, Kaiser C. Control of amino acid permease sorting in the late secretory pathway of Saccharomyces cerevisiae by SEC13, LST4, LST7 and LST8. Genetics. 1997;147:1569-84 pubmed
    The SEC13 gene was originally identified by temperature-sensitive mutations that block all protein transport from the ER to the Golgi...
  7. Elrod Erickson M, Kaiser C. Genes that control the fidelity of endoplasmic reticulum to Golgi transport identified as suppressors of vesicle budding mutations. Mol Biol Cell. 1996;7:1043-58 pubmed
    ..In a screen for suppressors of mutations in the essential COPII gene SEC13, we identified three genes (BST1, BST2/EMP24, and BST3) that negatively regulate COPII vesicle formation, ..
  8. Pryer N, Salama N, Schekman R, Kaiser C. Cytosolic Sec13p complex is required for vesicle formation from the endoplasmic reticulum in vitro. J Cell Biol. 1993;120:865-75 pubmed
    The SEC13 gene of Saccharomyces cerevisiae is required in vesicle biogenesis at a step before or concurrent with the release of transport vesicles from the ER membrane...
  9. Barlowe C, Orci L, Yeung T, Hosobuchi M, Hamamoto S, Salama N, et al. COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum. Cell. 1994;77:895-907 pubmed
    ..Because the overall cycle of budding driven by these two types of coats appears mechanistically similar, we propose that the coat structures be called COPI and COPII. ..
  10. Hsia K, Stavropoulos P, Blobel G, Hoelz A. Architecture of a coat for the nuclear pore membrane. Cell. 2007;131:1313-26 pubmed
    ..cylinder coating the pore membrane contains the previously characterized, elongated heptamer that harbors Sec13-Nup145C in its middle section. Strikingly, Sec13-Nup145C crystallizes as a hetero-octamer in two space groups...
  11. Siniossoglou S, Lutzmann M, Santos Rosa H, Leonard K, Mueller S, Aebi U, et al. Structure and assembly of the Nup84p complex. J Cell Biol. 2000;149:41-54 pubmed that a pool of green fluorescent protein-tagged Sec13p localizes to the nuclear pores in vivo, and identify sec13 mutant alleles that are synthetically lethal with nup85Delta and affect the localization of a green fluorescent ..
  12. Salama N, Yeung T, Schekman R. The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins. EMBO J. 1993;12:4073-82 pubmed
    b>SEC13 encodes a 33 kDa protein that participates in vesicle budding from the endoplasmic reticulum (ER)...
  13. Fath S, Mancias J, Bi X, Goldberg J. Structure and organization of coat proteins in the COPII cage. Cell. 2007;129:1325-36 pubmed
    ..The COPII coat consists of the Sec23/24-Sar1 complex that selects cargo and the Sec13/31 assembly unit that can polymerize into an octahedral cage and deform the membrane into a bud...
  14. Whittle J, Schwartz T. Structure of the Sec13-Sec16 edge element, a template for assembly of the COPII vesicle coat. J Cell Biol. 2010;190:347-61 pubmed publisher
    ..The ACE1 protein Sec31 and Sec13 make a 2:2 tetramer that forms the edge element of the COPII outer coat...
  15. Brohawn S, Leksa N, Spear E, Rajashankar K, Schwartz T. Structural evidence for common ancestry of the nuclear pore complex and vesicle coats. Science. 2008;322:1369-73 pubmed publisher
    ..We propose that the NPC scaffold, like vesicle coats, is composed of polygons with vertices and edges forming a membrane-proximal lattice that provides docking sites for additional nucleoporins. ..
  16. Liu H, Bretscher A. Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport. J Cell Biol. 1992;118:285-99 pubmed
    ..Combinations of the TPM1 disruption with sec13 or sec18 mutations, which affect early steps in the secretory pathway, block vesicle accumulation, while ..
  17. Sato M, Sato K, Nakano A. Evidence for the intimate relationship between vesicle budding from the ER and the unfolded protein response. Biochem Biophys Res Commun. 2002;296:560-7 pubmed
    ..The overexpression of IRE1 suppresses the sec mutants defective in vesicle budding from the ER but not others, highlighting a close relationship between the ER exit and the UPR. ..
  18. Antebi A, Fink G. The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol Biol Cell. 1992;3:633-54 pubmed
    ..Some of these interactions are modulated by changes in external Ca2+ concentrations. These results imply a global role for Ca2+ in the proper function of components governing transit and processing through the secretory pathway. ..
  19. Cleves A, Novick P, Bankaitis V. Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function. J Cell Biol. 1989;109:2939-50 pubmed
    ..On this basis, we suggest that SAC1p may represent one aspect of the mechanism whereby secretory and cytoskeletal activities are coordinated, so that proper spatial regulation of secretion might be achieved. ..
  20. Gilstring C, Melin Larsson M, Ljungdahl P. Shr3p mediates specific COPII coatomer-cargo interactions required for the packaging of amino acid permeases into ER-derived transport vesicles. Mol Biol Cell. 1999;10:3549-65 pubmed
  21. Kim D, Massey T, Sacher M, Pypaert M, Ferro Novick S. Sgf1p, a new component of the Sec34p/Sec35p complex. Traffic. 2001;2:820-30 pubmed
    ..Although an earlier study suggested that Sec34p (Grd20p) is not required for protein secretion, we show here that the sec34-2 and sec35-1 mutations lead to a pleiotropic block in the secretion of all proteins into the growth medium. ..
  22. D Arcangelo J, Crissman J, Pagant S, Čopič A, Latham C, Snapp E, et al. Traffic of p24 Proteins and COPII Coat Composition Mutually Influence Membrane Scaffolding. Curr Biol. 2015;25:1296-305 pubmed publisher
    ..Vesicles that contain such cargoes are also more dependent on scaffolding by Sec13p, and may serve as a model for large carrier formation in other systems. ..
  23. Kim S, Fernandez Martinez J, Sampathkumar P, Martel A, Matsui T, Tsuruta H, et al. Integrative structure-function mapping of the nucleoporin Nup133 suggests a conserved mechanism for membrane anchoring of the nuclear pore complex. Mol Cell Proteomics. 2014;13:2911-26 pubmed publisher
  24. Heidtman M, Chen C, Collins R, Barlowe C. A role for Yip1p in COPII vesicle biogenesis. J Cell Biol. 2003;163:57-69 pubmed
    ..We propose that Yip1p has a previously unappreciated role in COPII vesicle biogenesis. ..
  25. Hayakawa A, Babour A, Sengmanivong L, Dargemont C. Ubiquitylation of the nuclear pore complex controls nuclear migration during mitosis in S. cerevisiae. J Cell Biol. 2012;196:19-27 pubmed publisher
    ..This led to defects in nuclear segregation at the onset of mitosis. Thus, defining ubiquitylation of the yeast NPC highlights yet-unexplored functions of this essential organelle in cell division. ..
  26. Belden W, Barlowe C. Purification of functional Sec13p-Sec31p complex, a subunit of COPII coat. Methods Enzymol. 2001;329:438-43 pubmed
  27. Yorimitsu T, Sato K. Insights into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites. Mol Biol Cell. 2012;23:2930-42 pubmed publisher
    ..These features ensure prolonged COPII coat association within a preformed Sec16 cluster, which may lead to the formation of ERES. Our results indicate a mechanistic relationship between COPII coat assembly and ERES formation. ..
  28. Chabane S, Gachet E, Kepes F. Over-expression of the yeast BFR2 gene partially suppresses the growth defects induced by Brefeldin A and by four ER-to-Golgi mutations. Curr Genet. 1998;33:21-8 pubmed
    ..growth defect of four mutants blocked at the step of budding or docking of small vesicles en route to the Golgi (sec13-1, sec16-2, sec23-1, ypt1-1)...
  29. Panchaud N, P li Gulli M, De Virgilio C. SEACing the GAP that nEGOCiates TORC1 activation: evolutionary conservation of Rag GTPase regulation. Cell Cycle. 2013;12:2948-52 pubmed publisher
    ..we present genetic epistasis data, which show that SEACAT, the other SEAC subcomplex, containing Seh1, Sea2-4, and Sec13, antagonizes the GAP function of SEACIT...
  30. Teixeira M, Siniossoglou S, Podtelejnikov S, Benichou J, Mann M, Dujon B, et al. Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins. EMBO J. 1997;16:5086-97 pubmed
    ..These data suggest that N- and C-domains of Nup145p perform independent functions, and that the in vivo cleavage observed is of functional importance. ..
  31. Nagy V, Hsia K, Debler E, Kampmann M, Davenport A, Blobel G, et al. Structure of a trimeric nucleoporin complex reveals alternate oligomerization states. Proc Natl Acad Sci U S A. 2009;106:17693-8 pubmed publisher
    ..Here, we present the crystal structure of the heterotrimeric Sec13 x Nup145C x Nup84 complex, the centerpiece of the heptamer, at 3.2-A resolution...
  32. Chang H, Jesch S, Gaspar M, Henry S. Role of the unfolded protein response pathway in secretory stress and regulation of INO1 expression in Saccharomyces cerevisiae. Genetics. 2004;168:1899-913 pubmed
    ..Indeed, many of the Sec(-) mutants that had elevated UPR expression at semipermissive growth temperatures failed to achieve wild-type levels of INO1 expression under these same conditions. ..
  33. Fu L, Sztul E. Traffic-independent function of the Sar1p/COPII machinery in proteasomal sorting of the cystic fibrosis transmembrane conductance regulator. J Cell Biol. 2003;160:157-63 pubmed
    ..These findings reveal a new aspect of the degradative mechanism, and suggest functional crosstalk between the secretory and the degradative pathways...
  34. Roberg K, Crotwell M, Espenshade P, Gimeno R, Kaiser C. LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum. J Cell Biol. 1999;145:659-72 pubmed ten genes, designated LST (lethal with sec-thirteen), that were lethal in combination with the COPII mutation sec13-1...
  35. Flemming D, Thierbach K, Stelter P, Bottcher B, Hurt E. Precise mapping of subunits in multiprotein complexes by a versatile electron microscopy label. Nat Struct Mol Biol. 2010;17:775-8 pubmed publisher
    ..This tag is readily visible on single particles and becomes exceptionally distinct after image processing and classification. Thus, our method is applicable for the exact topological mapping of subunits in macromolecular complexes. ..
  36. Lederkremer G, Cheng Y, Petre B, Vogan E, Springer S, Schekman R, et al. Structure of the Sec23p/24p and Sec13p/31p complexes of COPII. Proc Natl Acad Sci U S A. 2001;98:10704-9 pubmed
    ..Putting together the architecture of these Sec complexes with the interactions between their subunits and the appearance of the coat in COPII-coated vesicles, we present a model for COPII coat organization...
  37. Copic A, Latham C, Horlbeck M, D Arcangelo J, Miller E. ER cargo properties specify a requirement for COPII coat rigidity mediated by Sec13p. Science. 2012;335:1359-62 pubmed publisher
    ..The outer coat complex, Sec13-Sec31, forms a scaffold that is thought to enforce curvature...
  38. Matsuoka K, Schekman R, Orci L, Heuser J. Surface structure of the COPII-coated vesicle. Proc Natl Acad Sci U S A. 2001;98:13705-9 pubmed
    ..crosslinking to phospholipids declined in order of protein recruitment to the coat: Sar1p > Sec23/24p > Sec13/31p...
  39. Yao W, Lutzmann M, Hurt E. A versatile interaction platform on the Mex67-Mtr2 receptor creates an overlap between mRNA and ribosome export. EMBO J. 2008;27:6-16 pubmed
    ..Thus, the Mex67-Mtr2 export receptor employs a versatile binding platform on its surface that could create a crosstalk between mRNA and ribosome export pathways. ..
  40. Chen C, Calero M, DeRegis C, Heidtman M, Barlowe C, Collins R. Genetic analysis of yeast Yip1p function reveals a requirement for Golgi-localized rab proteins and rab-Guanine nucleotide dissociation inhibitor. Genetics. 2004;168:1827-41 pubmed
  41. Stuwe T, von Borzyskowski L, Davenport A, Hoelz A. Molecular basis for the anchoring of proto-oncoprotein Nup98 to the cytoplasmic face of the nuclear pore complex. J Mol Biol. 2012;419:330-46 pubmed publisher
    ..Thus, the cytoplasmic filament network of the NPC is robust, consistent with its essential function in nucleocytoplasmic transport. ..
  42. Belden W, Barlowe C. Distinct roles for the cytoplasmic tail sequences of Emp24p and Erv25p in transport between the endoplasmic reticulum and Golgi complex. J Biol Chem. 2001;276:43040-8 pubmed
    ..The Erv25p tail sequence binds COPI and is responsible for returning this complex to the ER. ..
  43. Higashio H, Kohno K. A genetic link between the unfolded protein response and vesicle formation from the endoplasmic reticulum. Biochem Biophys Res Commun. 2002;296:568-74 pubmed
    ..These findings suggest that the activation of the UPR affects ER-to-Golgi transport via stimulation of COPII vesicle formation from the ER. ..