SEC1

Summary

Gene Symbol: SEC1
Description: Sec1p
Alias: Sec1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Brummer M, Kivinen K, Jantti J, Toikkanen J, Soderlund H, Keranen S. Characterization of the sec1-1 and sec1-11 mutations. Yeast. 2001;18:1525-36 pubmed
    ..Compared to the wild-type Sec1p the mutant proteins are less abundant even at the permissive temperature...
  2. Castillo Flores A, Weinberger A, Robinson M, Gerst J. Mso1 is a novel component of the yeast exocytic SNARE complex. J Biol Chem. 2005;280:34033-41 pubmed
    ..Another protein, Sec1, binds to the SNARE complex to facilitate assembly...
  3. Zhang X, Wang P, Gangar A, Zhang J, Brennwald P, TerBush D, et al. Lethal giant larvae proteins interact with the exocyst complex and are involved in polarized exocytosis. J Cell Biol. 2005;170:273-83 pubmed
    ..We propose that, although Lgl is broadly distributed in the cells, its localized interaction with the exocyst and kinetic activation are important for the establishment and reenforcement of cell polarity. ..
  4. Weber M, Chernov K, Turakainen H, Wohlfahrt G, Pajunen M, Savilahti H, et al. Mso1p regulates membrane fusion through interactions with the putative N-peptide-binding area in Sec1p domain 1. Mol Biol Cell. 2010;21:1362-74 pubmed publisher
    b>Sec1p/Munc18 (SM) family proteins regulate SNARE complex function in membrane fusion through their interactions with syntaxins...
  5. Carr C, Grote E, Munson M, Hughson F, Novick P. Sec1p binds to SNARE complexes and concentrates at sites of secretion. J Cell Biol. 1999;146:333-44 pubmed
    ..We demonstrate that yeast Sec1p coprecipitates not only the syntaxin homologue Ssop, but also the other two exocytic SNAREs (Sec9p and Sncp) in ..
  6. Finger F, Novick P. Synthetic interactions of the post-Golgi sec mutations of Saccharomyces cerevisiae. Genetics. 2000;156:943-51 pubmed
    ..The significance of these results is discussed in the context of both secretory pathway function and the utility of synthetic lethality studies and their interpretation. ..
  7. Grote E, Carr C, Novick P. Ordering the final events in yeast exocytosis. J Cell Biol. 2000;151:439-52 pubmed
    ..that block either secretory vesicle delivery or tethering prevent SNARE complex assembly and the localization of Sec1p, a SNARE complex binding protein, to sites of secretion...
  8. Hashizume K, Cheng Y, Hutton J, Chiu C, Carr C. Yeast Sec1p functions before and after vesicle docking. Mol Biol Cell. 2009;20:4673-85 pubmed publisher
    ..Yeast Sec1p seems to function only after vesicles are docked and SNARE complexes are assembled...
  9. Weber Boyvat M, Aro N, Chernov K, Nyman T, Jantti J. Sec1p and Mso1p C-terminal tails cooperate with the SNAREs and Sec4p in polarized exocytosis. Mol Biol Cell. 2011;22:230-44 pubmed publisher
    ..The Saccharomyces cerevisiae Sec1p has a C-terminal tail that is missing in its mammalian homologues...

More Information

Publications39

  1. Elbert M, Rossi G, Brennwald P. The yeast par-1 homologs kin1 and kin2 show genetic and physical interactions with components of the exocytic machinery. Mol Biol Cell. 2005;16:532-49 pubmed
    ..In summary, we report the finding that yeast Par-1 counterparts are associated with and regulate the function of the exocytic apparatus via phosphorylation of Sec9. ..
  2. Knop M, Miller K, Mazza M, Feng D, Weber M, Keränen S, et al. Molecular interactions position Mso1p, a novel PTB domain homologue, in the interface of the exocyst complex and the exocytic SNARE machinery in yeast. Mol Biol Cell. 2005;16:4543-56 pubmed
    In this study, we have analyzed the association of the Sec1p interacting protein Mso1p with the membrane fusion machinery in yeast. We show that Mso1p is essential for vesicle fusion during prospore membrane formation...
  3. Aalto M, Jantti J, Ostling J, Keranen S, Ronne H. Mso1p: a yeast protein that functions in secretion and interacts physically and genetically with Sec1p. Proc Natl Acad Sci U S A. 1997;94:7331-6 pubmed
    The yeast Sec1p protein functions in the docking of secretory transport vesicles to the plasma membrane...
  4. Aalto M, Ronne H, Keranen S. Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane proteins that function in vesicular transport. EMBO J. 1993;12:4095-104 pubmed
    The yeast SEC1 gene encodes a hydrophilic protein that functions at the terminal stage in secretion...
  5. Wiederkehr A, De Craene J, Ferro Novick S, Novick P. Functional specialization within a vesicle tethering complex: bypass of a subset of exocyst deletion mutants by Sec1p or Sec4p. J Cell Biol. 2004;167:875-87 pubmed
    ..for growth or secretion when either the upstream activator rab, Sec4p, or the downstream SNARE-binding component, Sec1p, are overproduced...
  6. Grosshans B, Andreeva A, Gangar A, Niessen S, Yates J, Brennwald P, et al. The yeast lgl family member Sro7p is an effector of the secretory Rab GTPase Sec4p. J Cell Biol. 2006;172:55-66 pubmed
    ..Genetic data support our conclusion that Sro7p functions downstream of Sec4p and further imply that Sro7p and the exocyst share partially overlapping functions, possibly in SNARE regulation. ..
  7. Lehman K, Rossi G, Adamo J, Brennwald P. Yeast homologues of tomosyn and lethal giant larvae function in exocytosis and are associated with the plasma membrane SNARE, Sec9. J Cell Biol. 1999;146:125-40 pubmed
    ..Taken together, our studies suggest that members of the lethal giant larvae/tomosyn/Sro7 family play an important role in polarized exocytosis by regulating SNARE function on the plasma membrane. ..
  8. Scott B, Van Komen J, Irshad H, Liu S, Wilson K, McNew J. Sec1p directly stimulates SNARE-mediated membrane fusion in vitro. J Cell Biol. 2004;167:75-85 pubmed
    ..We have examined the role of Sec1p in the regulation of post-Golgi secretion in Saccharomyces cerevisiae...
  9. Togneri J, Cheng Y, Munson M, Hughson F, Carr C. Specific SNARE complex binding mode of the Sec1/Munc-18 protein, Sec1p. Proc Natl Acad Sci U S A. 2006;103:17730-5 pubmed
    ..In support of an activation role, SM proteins bind directly to their cognate syntaxins. An exception is the yeast Sec1p, which does not bind the yeast plasma-membrane syntaxin, Sso1p...
  10. Finger F, Novick P. Sec3p is involved in secretion and morphogenesis in Saccharomyces cerevisiae. Mol Biol Cell. 1997;8:647-62 pubmed
    ..This suggests that SEC gene products are involved in determining the bud site and is consistent with a role for Sec3p in determining the correct site of exocytosis. ..
  11. Yuan S, Nie W, He F, Jia Z, Gao X. Kin2, the Budding Yeast Ortholog of Animal MARK/PAR-1 Kinases, Localizes to the Sites of Polarized Growth and May Regulate Septin Organization and the Cell Wall. PLoS ONE. 2016;11:e0153992 pubmed publisher
    ..We speculate that Kin2 may be regulated by Bmh1, Rho3, or Pea2 in vivo. Our study provides new insight in the localization, function, and regulation of Kin2. ..
  12. Prigent M, Boy Marcotte E, Chesneau L, Gibson K, Dupre Crochet S, Tisserand H, et al. The RabGAP proteins Gyp5p and Gyl1p recruit the BAR domain protein Rvs167p for polarized exocytosis. Traffic. 2011;12:1084-97 pubmed publisher
  13. Dubuke M, Maniatis S, Shaffer S, Munson M. The Exocyst Subunit Sec6 Interacts with Assembled Exocytic SNARE Complexes. J Biol Chem. 2015;290:28245-56 pubmed publisher
    ..Together, these results suggest a new model in which Sec6 promotes SNARE complex assembly, similar to the role proposed for other tether subunit-SNARE interactions. ..
  14. Stalder D, Novick P. The casein kinases Yck1p and Yck2p act in the secretory pathway, in part, by regulating the Rab exchange factor Sec2p. Mol Biol Cell. 2016;27:686-701 pubmed publisher
    ..This promotes Sec2p function by stimulating its interaction with Sec15p. Finally, Sec2p is dephosphorylated very late in the exocytic reaction to facilitate recycling. ..
  15. Grote E, Baba M, Ohsumi Y, Novick P. Geranylgeranylated SNAREs are dominant inhibitors of membrane fusion. J Cell Biol. 2000;151:453-66 pubmed
    ..Mutant SNARE complexes recruit GFP-Sec1p to sites of exocytosis and can be disassembled by the Sec18p ATPase...
  16. Bareth B, Nikolov M, Lorenzi I, Hildenbeutel M, Mick D, Helbig C, et al. Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1. Mol Biol Cell. 2016;27:1570-80 pubmed publisher
    ..Our analyses show that Oms1 participates in cytochrome c oxidase assembly by stabilizing newly synthesized Cox1. ..
  17. Luo G, Zhang J, Guo W. The role of Sec3p in secretory vesicle targeting and exocyst complex assembly. Mol Biol Cell. 2014;25:3813-22 pubmed publisher
    ..Our study helps to establish the role of the exocyst subunits in tethering and allows the investigation of the mechanisms that regulate vesicle tethering during exocytosis. ..
  18. Wu C, Chen Y, Gartenberg M. Targeted sister chromatid cohesion by Sir2. PLoS Genet. 2011;7:e1002000 pubmed publisher
    ..Taken together, these data define a unique role for Sir2 in cohesion of silent chromatin that is distinct from the enzyme's role as a histone deacetylase. ..
  19. Ossig R, Schmitt H, de Groot B, Riedel D, Keranen S, Ronne H, et al. Exocytosis requires asymmetry in the central layer of the SNARE complex. EMBO J. 2000;19:6000-10 pubmed
    ..We conclude that the asymmetric arrangement of the polar amino acids in the central layer is essential for normal function of SNAREs in membrane fusion. ..
  20. Grote E. Secretion is required for late events in the cell-fusion pathway of mating yeast. J Cell Sci. 2010;123:1902-12 pubmed publisher
  21. Demmel L, Beck M, Klose C, Schlaitz A, Gloor Y, Hsu P, et al. Nucleocytoplasmic shuttling of the Golgi phosphatidylinositol 4-kinase Pik1 is regulated by 14-3-3 proteins and coordinates Golgi function with cell growth. Mol Biol Cell. 2008;19:1046-61 pubmed publisher
    ..These data suggest a role of Pik1p nucleocytoplasmic shuttling in coordination of biosynthetic transport from the Golgi with nutrient signaling. ..
  22. Antebi A, Fink G. The yeast Ca(2+)-ATPase homologue, PMR1, is required for normal Golgi function and localizes in a novel Golgi-like distribution. Mol Biol Cell. 1992;3:633-54 pubmed
    ..Some of these interactions are modulated by changes in external Ca2+ concentrations. These results imply a global role for Ca2+ in the proper function of components governing transit and processing through the secretory pathway. ..
  23. Medkova M, France Y, Coleman J, Novick P. The rab exchange factor Sec2p reversibly associates with the exocyst. Mol Biol Cell. 2006;17:2757-69 pubmed
    ..The mislocalization of Sec2p mutants results from a failure to be released from Sec15p, blocking this recycling pathway. ..
  24. Cleves A, Novick P, Bankaitis V. Mutations in the SAC1 gene suppress defects in yeast Golgi and yeast actin function. J Cell Biol. 1989;109:2939-50 pubmed
    ..On this basis, we suggest that SAC1p may represent one aspect of the mechanism whereby secretory and cytoskeletal activities are coordinated, so that proper spatial regulation of secretion might be achieved. ..
  25. Tomeo M, Palermo L, Tove S, Parks L. A conditional sterol esterification defect in yeast having either a sec1 or sec5 mutation in the secretory pathway. Yeast. 1997;13:449-62 pubmed
    Two temperature-conditional secretory mutations, sec1 and sec5, cause the accumulation of post-Golgi vesicles when strains containing these mutations are grown at 37 degrees C...
  26. Liu D, Novick P. Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p. J Cell Biol. 2014;207:59-72 pubmed publisher
    ..Similar to Sec3p, the actin-independent localization of Exo70p requires a synergistic interaction with the phosphoinositide PI(4,5)P2. ..
  27. Roth D, Guo W, Novick P. Dominant negative alleles of SEC10 reveal distinct domains involved in secretion and morphogenesis in yeast. Mol Biol Cell. 1998;9:1725-39 pubmed
  28. Aronov S, Gelin Licht R, Zipor G, Haim L, Safran E, Gerst J. mRNAs encoding polarity and exocytosis factors are cotransported with the cortical endoplasmic reticulum to the incipient bud in Saccharomyces cerevisiae. Mol Cell Biol. 2007;27:3441-55 pubmed
    ..Bud-localized mRNAs, including ASH1, were found to cofractionate with ER microsomes in a She2- and Sec3-dependent manner; thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast. ..
  29. Morgera F, Sallah M, Dubuke M, Gandhi P, Brewer D, Carr C, et al. Regulation of exocytosis by the exocyst subunit Sec6 and the SM protein Sec1. Mol Biol Cell. 2012;23:337-46 pubmed publisher
    ..Here we show another facet of Sec6 function-it directly binds Sec1, another SNARE regulator, but of the Sec1/Munc18 family...
  30. Couve A, Gerst J. Yeast Snc proteins complex with Sec9. Functional interactions between putative SNARE proteins. J Biol Chem. 1994;269:23391-4 pubmed
    ..We suggest, then, that recognition of the plasma membrane by secretory vesicles may involve the formation of a Snc-Sec9 complex and that this interaction has evolved as a fundamental step in secretory processes. ..