Gene Symbol: SAM35
Description: SAM complex subunit SAM35
Alias: FMP20, TOB38, TOM38, SAM complex subunit SAM35
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Meisinger C, Pfannschmidt S, Rissler M, Milenkovic D, Becker T, Stojanovski D, et al. The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria. EMBO J. 2007;26:2229-39 pubmed
    ..It has been assumed that the SAM(core) complex with the subunits Sam35, Sam37 and Sam50 represents the last import stage common to all beta-barrel proteins, followed by splitting in a ..
  2. Waizenegger T, Habib S, Lech M, Mokranjac D, Paschen S, Hell K, et al. Tob38, a novel essential component in the biogenesis of beta-barrel proteins of mitochondria. EMBO Rep. 2004;5:704-9 pubmed
    ..Known constituents of this complex are Tob55 and Mas37. We identified a novel component, Tob38. It is essential for viability of yeast and the function of the TOB complex...
  3. Thornton N, Stroud D, Milenkovic D, Guiard B, Pfanner N, Becker T. Two modular forms of the mitochondrial sorting and assembly machinery are involved in biogenesis of alpha-helical outer membrane proteins. J Mol Biol. 2010;396:540-9 pubmed publisher
    ..We suggest that the modular composition of the SAM complex provides a flexible platform to integrate the sorting pathways of different precursor proteins and to promote their assembly into oligomeric complexes. ..
  4. Kutik S, Stojanovski D, Becker L, Becker T, Meinecke M, Krüger V, et al. Dissecting membrane insertion of mitochondrial beta-barrel proteins. Cell. 2008;132:1011-24 pubmed publisher
    ..The SAM complex contains two proteins essential for cell viability, the channel-forming Sam50 and Sam35. We have identified the sorting signal of mitochondrial beta-barrel proteins that is universal in all eukaryotic ..
  5. Ishikawa D, Yamamoto H, Tamura Y, Moritoh K, Endo T. Two novel proteins in the mitochondrial outer membrane mediate beta-barrel protein assembly. J Cell Biol. 2004;166:621-7 pubmed
    ..Here we report two novel mitochondrial outer membrane proteins in yeast, Tom13 and Tom38/Sam35, that mediate assembly of mitochondrial beta-barrel proteins, Tom40, and/or porin in the outer membrane...
  6. Yamano K, Tanaka Yamano S, Endo T. Mdm10 as a dynamic constituent of the TOB/SAM complex directs coordinated assembly of Tom40. EMBO Rep. 2010;11:187-93 pubmed publisher
    ..These results show that Mdm10 regulates the timing of release of unassembled Tom40 from the TOB complex, to facilitate its coordinated assembly into the TOM40 complex. ..
  7. Yamano K, Tanaka Yamano S, Endo T. Tom7 regulates Mdm10-mediated assembly of the mitochondrial import channel protein Tom40. J Biol Chem. 2010;285:41222-31 pubmed publisher
    ..These results collectively show that Tom7 recruits Mdm10, enhancing its association with the MMM1 complex, to regulate timing of the release of Tom40 from the TOB complex for subsequent assembly into the TOM40 complex. ..
  8. Becker T, Wenz L, Thornton N, Stroud D, Meisinger C, Wiedemann N, et al. Biogenesis of mitochondria: dual role of Tom7 in modulating assembly of the preprotein translocase of the outer membrane. J Mol Biol. 2011;405:113-24 pubmed publisher
    ..Thus, Tom7 modulates the biogenesis of topologically different proteins, the ?-barrel forming protein Tom40 and Tom22 that contains a transmembrane ?-helix. ..
  9. Korner C, Barrera M, Dukanovic J, Eydt K, Harner M, Rabl R, et al. The C-terminal domain of Fcj1 is required for formation of crista junctions and interacts with the TOB/SAM complex in mitochondria. Mol Biol Cell. 2012;23:2143-55 pubmed publisher
    ..These results assign novel functions to both the C-terminal domain of Fcj1 and the TOB/SAM complex. ..

More Information


  1. Habib S, Waizenegger T, Lech M, Neupert W, Rapaport D. Assembly of the TOB complex of mitochondria. J Biol Chem. 2005;280:6434-40 pubmed
    ..The other two components of the TOB complex are Tob38, which builds a functional TOB core complex with Tob55, and Mas37, a peripheral member of the complex...
  2. Chan N, Lithgow T. The peripheral membrane subunits of the SAM complex function codependently in mitochondrial outer membrane biogenesis. Mol Biol Cell. 2008;19:126-36 pubmed
    ..bacterial outer membranes, but the eukaryotic SAM complex is distinguished by two peripheral subunits, Sam37 and Sam35, that sit on the cytosolic face of the complex...
  3. Stojanovski D, Guiard B, Kozjak Pavlovic V, Pfanner N, Meisinger C. Alternative function for the mitochondrial SAM complex in biogenesis of alpha-helical TOM proteins. J Cell Biol. 2007;179:881-93 pubmed
    ..Thus, the substrate specificity of SAM is not restricted to beta-barrel proteins but also includes the majority of alpha-helical Tom proteins. ..
  4. Milenkovic D, Kozjak V, Wiedemann N, Lohaus C, Meyer H, Guiard B, et al. Sam35 of the mitochondrial protein sorting and assembly machinery is a peripheral outer membrane protein essential for cell viability. J Biol Chem. 2004;279:22781-5 pubmed
    ..Here we report the identification of Sam35, the first peripheral mitochondrial outer membrane protein that is essential for cell viability...
  5. Stroud D, Becker T, Qiu J, Stojanovski D, Pfannschmidt S, Wirth C, et al. Biogenesis of mitochondrial ?-barrel proteins: the POTRA domain is involved in precursor release from the SAM complex. Mol Biol Cell. 2011;22:2823-33 pubmed publisher
    ..These results indicate the POTRA domain of Sam50 is not essential for recognition of ?-barrel precursors but functions in a subsequent step to promote the release of precursor proteins from the SAM complex. ..
  6. Zerbes R, Bohnert M, Stroud D, von der Malsburg K, Kram A, Oeljeklaus S, et al. Role of MINOS in mitochondrial membrane architecture: cristae morphology and outer membrane interactions differentially depend on mitofilin domains. J Mol Biol. 2012;422:183-91 pubmed publisher
  7. Bohnert M, Wenz L, Zerbes R, Horvath S, Stroud D, von der Malsburg K, et al. Role of mitochondrial inner membrane organizing system in protein biogenesis of the mitochondrial outer membrane. Mol Biol Cell. 2012;23:3948-56 pubmed publisher
    ..We conclude that MINOS interacts with TOM and SAM independently and that the core subunit mitofilin is involved in biogenesis of outer membrane ?-barrel proteins. ..
  8. Wenz L, Ellenrieder L, Qiu J, Bohnert M, Zufall N, van der Laan M, et al. Sam37 is crucial for formation of the mitochondrial TOM-SAM supercomplex, thereby promoting β-barrel biogenesis. J Cell Biol. 2015;210:1047-54 pubmed publisher
    ..The SAM core complex contains the channel protein Sam50, which cooperates with Sam35 in precursor recognition, and the peripheral membrane protein Sam37...