Gene Symbol: SAM2
Description: methionine adenosyltransferase SAM2
Alias: ETH2, methionine adenosyltransferase SAM2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Schwer B, Saha N, Mao X, Chen H, Shuman S. Structure-function analysis of yeast mRNA cap methyltransferase and high-copy suppression of conditional mutants by AdoMet synthase and the ubiquitin conjugating enzyme Cdc34p. Genetics. 2000;155:1561-76 pubmed
    ..The identification of the yeast genes SAM2 and SAM1, which encode AdoMet synthase, as abd1 suppressors suggests that Abd1p function can be modulated by ..
  2. Kuras L, Rouillon A, Lee T, Barbey R, Tyers M, Thomas D. Dual regulation of the met4 transcription factor by ubiquitin-dependent degradation and inhibition of promoter recruitment. Mol Cell. 2002;10:69-80 pubmed
  3. Ogawa T, Tsubakiyama R, Kanai M, Koyama T, Fujii T, Iefuji H, et al. Stimulating S-adenosyl-l-methionine synthesis extends lifespan via activation of AMPK. Proc Natl Acad Sci U S A. 2016;113:11913-11918 pubmed
    ..05% glucose) conditions. Thus, our data uncovered molecular links between Met metabolites and lifespan, suggesting a unique function of AdoMet as a reservoir of Met and ATP for cell survival. ..
  4. Hickman M, Petti A, Ho Shing O, Silverman S, McIsaac R, Lee T, et al. Coordinated regulation of sulfur and phospholipid metabolism reflects the importance of methylation in the growth of yeast. Mol Biol Cell. 2011;22:4192-204 pubmed publisher
    ..Our results show that rapidly growing cells require significant methylation, likely for the biosynthesis of phospholipids. ..
  5. Antoniewski J, Robichon Szulmajster H. Biosynthesis of methionine and its control in wild type and regulatory mutants of Saccharomyces cerevisiae. Biochimie. 1973;55:529-39 pubmed
  6. Chen H, Wang Z, Wang Z, Dou J, Zhou C. Improving methionine and ATP availability by MET6 and SAM2 co-expression combined with sodium citrate feeding enhanced SAM accumulation in Saccharomyces cerevisiae. World J Microbiol Biotechnol. 2016;32:56 pubmed publisher
    ..cerevisiae CGMCC 2842 (wild type), improvement of methionine and ATP availability through MET6 and SAM2 co-expression combined with sodium citrate feeding was investigated here...
  7. Moriel Carretero M, Tous C, Aguilera A. Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1. Proc Natl Acad Sci U S A. 2011;108:15300-5 pubmed publisher
    ..Our results provide evidence for a role of chaperones in NER and transcription, with implications in cancer and TFIIH-associated syndromes. ..
  8. Kerr S, Azzouz N, Fuchs S, Collart M, Strahl B, Corbett A, et al. The Ccr4-Not complex interacts with the mRNA export machinery. PLoS ONE. 2011;6:e18302 pubmed publisher
    ..These results shed further insight into the biological functions of Ccr4-Not and suggest that this complex is involved in all aspects of mRNA biogenesis, from the regulation of transcription to mRNA export and turnover. ..
  9. Zheng S, Shuman S, Schwer B. Sinefungin resistance of Saccharomyces cerevisiae arising from Sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase. Nucleic Acids Res. 2007;35:6895-903 pubmed
    ..These results are consistent with the proposal that mRNA cap methylation is a principal target of sinefungin's bioactivity. ..

More Information


  1. Ranish J, Yi E, Leslie D, Purvine S, Goodlett D, Eng J, et al. The study of macromolecular complexes by quantitative proteomics. Nat Genet. 2003;33:349-55 pubmed
  2. Luo Y, Yuan Z, Luo G, Zhao F. Expression of secreted His-tagged S-adenosylmethionine synthetase in the methylotrophic yeast Pichia pastoris and its characterization, one-step purification, and immobilization. Biotechnol Prog. 2008;24:214-20 pubmed
    ..a simple and effective way to enzymatically synthesize and produce SAM, a soluble form of SAM synthetase encoded by SAM2 from Saccharomyces cerevisiae was successfully produced at high level ( approximately 200 mg/L) by the recombinant ..
  3. Bockhorn J, Balar B, He D, Seitomer E, Copeland P, Kinzy T. Genome-wide screen of Saccharomyces cerevisiae null allele strains identifies genes involved in selenomethionine resistance. Proc Natl Acad Sci U S A. 2008;105:17682-7 pubmed publisher
    ..The CYS3 deletion strain provides advantages for an easy and cost-effective method to prepare SeMet-substituted protein in yeast and perhaps other eukaryotic systems. ..
  4. Dato L, Berterame N, Ricci M, Paganoni P, Palmieri L, Porro D, et al. Changes in SAM2 expression affect lactic acid tolerance and lactic acid production in Saccharomyces cerevisiae. Microb Cell Fact. 2014;13:147 pubmed publisher
    ..Western blot analyses showed that lactic acid exposure correlates with an increase in protein levels. The SAM2 gene was then overexpressed and deleted in laboratory strains...
  5. Li S, Swanson S, Gogol M, Florens L, Washburn M, Workman J, et al. Serine and SAM Responsive Complex SESAME Regulates Histone Modification Crosstalk by Sensing Cellular Metabolism. Mol Cell. 2015;60:408-21 pubmed publisher
    ..This leads to auto-regulation of PYK1 expression. Thus, our study provides insights into the mechanism of regulating gene expression, responding to cellular metabolism via chromatin modifications. ..
  6. Eschrich D, Buchhaupt M, K├Âtter P, Entian K. Nep1p (Emg1p), a novel protein conserved in eukaryotes and archaea, is involved in ribosome biogenesis. Curr Genet. 2002;40:326-38 pubmed
  7. Malkowski M, Quartley E, Friedman A, Babulski J, Kon Y, Wolfley J, et al. Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing. Proc Natl Acad Sci U S A. 2007;104:6678-83 pubmed
    ..sam1(-) sam2(-) mutants, in which the conversion of methionine to S-adenosylmethionine is blocked, exhibit reduced ..
  8. Harrison R, Papp B, Pal C, Oliver S, Delneri D. Plasticity of genetic interactions in metabolic networks of yeast. Proc Natl Acad Sci U S A. 2007;104:2307-12 pubmed
    ..Our work supports the view that functional redundancy may be more apparent than real, and it offers a unified framework for the evolution of environmental adaptation and mutational robustness. ..
  9. Cherest H, Surdin Kerjan Y. S-adenosyl methionine requiring mutants in Saccharomyces cerevisiae: evidences for the existence of two methionine adenosyl transferases. Mol Gen Genet. 1978;163:153-67 pubmed
    ..In addition, SAM1 and SAM2 loci have been identified respectively with the ETH-10 and ETH2 loci previously described. Biochemical evidences corroborate the genetic results...