Gene Symbol: SAM1
Description: methionine adenosyltransferase SAM1
Alias: ETH10, methionine adenosyltransferase SAM1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Schwer B, Saha N, Mao X, Chen H, Shuman S. Structure-function analysis of yeast mRNA cap methyltransferase and high-copy suppression of conditional mutants by AdoMet synthase and the ubiquitin conjugating enzyme Cdc34p. Genetics. 2000;155:1561-76 pubmed
    ..The identification of the yeast genes SAM2 and SAM1, which encode AdoMet synthase, as abd1 suppressors suggests that Abd1p function can be modulated by changes in the ..
  2. Ranish J, Yi E, Leslie D, Purvine S, Goodlett D, Eng J, et al. The study of macromolecular complexes by quantitative proteomics. Nat Genet. 2003;33:349-55 pubmed
  3. Cherest H, Surdin Kerjan Y. S-adenosyl methionine requiring mutants in Saccharomyces cerevisiae: evidences for the existence of two methionine adenosyl transferases. Mol Gen Genet. 1978;163:153-67 pubmed
    ..One class corresponds to the simultaneous occurrence of mutations at two unlinked loci SAM1 and SAM2 and presents a strict SAM requirement for growth on any medium...
  4. Thomas D, Surdin Kerjan Y. SAM1, the structural gene for one of the S-adenosylmethionine synthetases in Saccharomyces cerevisiae. Sequence and expression. J Biol Chem. 1987;262:16704-9 pubmed
    Saccharomyces cerevisiae contains two genes, SAM1 and SAM2, encoding functional S-adenosylmethionine synthetases. The gene SAM1 was isolated by functional complementation of a double mutant of S...
  5. Malkowski M, Quartley E, Friedman A, Babulski J, Kon Y, Wolfley J, et al. Blocking S-adenosylmethionine synthesis in yeast allows selenomethionine incorporation and multiwavelength anomalous dispersion phasing. Proc Natl Acad Sci U S A. 2007;104:6678-83 pubmed
    ..b>sam1(-) sam2(-) mutants, in which the conversion of methionine to S-adenosylmethionine is blocked, exhibit reduced ..
  6. Bockhorn J, Balar B, He D, Seitomer E, Copeland P, Kinzy T. Genome-wide screen of Saccharomyces cerevisiae null allele strains identifies genes involved in selenomethionine resistance. Proc Natl Acad Sci U S A. 2008;105:17682-7 pubmed publisher
    ..The CYS3 deletion strain provides advantages for an easy and cost-effective method to prepare SeMet-substituted protein in yeast and perhaps other eukaryotic systems. ..
  7. Kitajima T, Chiba Y, Jigami Y. Mutation of high-affinity methionine permease contributes to selenomethionyl protein production in Saccharomyces cerevisiae. Appl Environ Microbiol. 2010;76:6351-9 pubmed publisher
    ..Based on these results, we conclude that the mup1 mutant would be useful for the preparation of selenomethionyl proteins for X-ray crystallography. ..
  8. Kerr S, Azzouz N, Fuchs S, Collart M, Strahl B, Corbett A, et al. The Ccr4-Not complex interacts with the mRNA export machinery. PLoS ONE. 2011;6:e18302 pubmed publisher
    ..These results shed further insight into the biological functions of Ccr4-Not and suggest that this complex is involved in all aspects of mRNA biogenesis, from the regulation of transcription to mRNA export and turnover. ..
  9. Li S, Swanson S, Gogol M, Florens L, Washburn M, Workman J, et al. Serine and SAM Responsive Complex SESAME Regulates Histone Modification Crosstalk by Sensing Cellular Metabolism. Mol Cell. 2015;60:408-21 pubmed publisher
    ..This leads to auto-regulation of PYK1 expression. Thus, our study provides insights into the mechanism of regulating gene expression, responding to cellular metabolism via chromatin modifications. ..

More Information


  1. Ogawa T, Tsubakiyama R, Kanai M, Koyama T, Fujii T, Iefuji H, et al. Stimulating S-adenosyl-l-methionine synthesis extends lifespan via activation of AMPK. Proc Natl Acad Sci U S A. 2016;113:11913-11918 pubmed
    ..05% glucose) conditions. Thus, our data uncovered molecular links between Met metabolites and lifespan, suggesting a unique function of AdoMet as a reservoir of Met and ATP for cell survival. ..
  2. Cherest H, Surdin Kerjan Y, Antoniewski J, De Robichon Szulmajster H. Effects of regulatory mutations upon methionine biosynthesis in Saccharomyces cerevisiae: loci eth2-eth3-eth10. J Bacteriol. 1973;115:1084-93 pubmed
    The effects of mutations occurring at three independent loci, eth2, eth3, and eth10, were studied on the basis of several criteria: level of resistance towards two methionine analogues (ethionine and selenomethionine), pool sizes of free ..
  3. Harrison R, Papp B, Pal C, Oliver S, Delneri D. Plasticity of genetic interactions in metabolic networks of yeast. Proc Natl Acad Sci U S A. 2007;104:2307-12 pubmed
    ..Our work supports the view that functional redundancy may be more apparent than real, and it offers a unified framework for the evolution of environmental adaptation and mutational robustness. ..
  4. Zheng S, Shuman S, Schwer B. Sinefungin resistance of Saccharomyces cerevisiae arising from Sam3 mutations that inactivate the AdoMet transporter or from increased expression of AdoMet synthase plus mRNA cap guanine-N7 methyltransferase. Nucleic Acids Res. 2007;35:6895-903 pubmed
    ..These results are consistent with the proposal that mRNA cap methylation is a principal target of sinefungin's bioactivity. ..
  5. Mirzaei H, Rogers R, Grimes B, Eng J, Aderem A, Aebersold R. Characterizing the connectivity of poly-ubiquitin chains by selected reaction monitoring mass spectrometry. Mol Biosyst. 2010;6:2004-14 pubmed publisher
    ..cerevisiae. We then applied the method to detect toxin induced changes in the poly-ubiquitination profile in complex and enriched protein samples. ..