SAE2

Summary

Gene Symbol: SAE2
Description: ssDNA endodeoxyribonuclease SAE2
Alias: COM1, ssDNA endodeoxyribonuclease SAE2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Matsuzaki K, Terasawa M, Iwasaki D, Higashide M, Shinohara M. Cyclin-dependent kinase-dependent phosphorylation of Lif1 and Sae2 controls imprecise nonhomologous end joining accompanied by double-strand break resection. Genes Cells. 2012;17:473-93 pubmed publisher
    ..in micro-homology-dependent end joining associated with producing single-stranded DSB ends that are formed by Sae2 as early intermediates in the HR pathway...
  2. Cannavo E, Cejka P. Sae2 promotes dsDNA endonuclease activity within Mre11-Rad50-Xrs2 to resect DNA breaks. Nature. 2014;514:122-5 pubmed publisher
    ..Using purified Saccharomyces cerevisiae proteins, we show that Sae2 promotes dsDNA-specific endonuclease activity by the Mre11 subunit within the MRX complex...
  3. Cartagena Lirola H, Guerini I, Viscardi V, Lucchini G, Longhese M. Budding Yeast Sae2 is an In Vivo Target of the Mec1 and Tel1 Checkpoint Kinases During Meiosis. Cell Cycle. 2006;5:1549-59 pubmed
    ..We show that the Saccharomyces cerevisiae Sae2 protein, known to be involved in processing meiotic DSBs, is phosphorylated periodically during the meiotic cycle...
  4. Chen H, Lisby M, Symington L. RPA coordinates DNA end resection and prevents formation of DNA hairpins. Mol Cell. 2013;50:589-600 pubmed publisher
    ..Thus, RPA is required to generate ssDNA, and also to protect ssDNA from degradation and inappropriate annealing that could lead to genome rearrangements. ..
  5. Huertas P, Cortes Ledesma F, Sartori A, Aguilera A, Jackson S. CDK targets Sae2 to control DNA-end resection and homologous recombination. Nature. 2008;455:689-92 pubmed publisher
    ..in Saccharomyces cerevisiae results from the phosphorylation by CDK of an evolutionarily conserved motif in the Sae2 protein...
  6. Westmoreland J, Resnick M. Coincident resection at both ends of random, ?-induced double-strand breaks requires MRX (MRN), Sae2 (Ctp1), and Mre11-nuclease. PLoS Genet. 2013;9:e1003420 pubmed publisher
    ..Surprisingly, Sae2 (Ctp1/CtIP) and Mre11 nuclease-deficient mutants have similar responses, although there is less impact on repair...
  7. Deng S, Gibb B, de Almeida M, Greene E, Symington L. RPA antagonizes microhomology-mediated repair of DNA double-strand breaks. Nat Struct Mol Biol. 2014;21:405-12 pubmed publisher
    ..We propose that the helix-destabilizing activity of RPA channels ssDNA intermediates from mutagenic MMEJ to error-free homologous recombination, thus preserving genome integrity. ..
  8. Bonetti D, Martina M, Clerici M, Lucchini G, Longhese M. Multiple pathways regulate 3' overhang generation at S. cerevisiae telomeres. Mol Cell. 2009;35:70-81 pubmed publisher
    ..We show by an inducible short telomere assay that Sae2 and the Sgs1 RecQ helicase control two distinct but partially complementary pathways for nucleolytic processing of ..
  9. Mimitou E, Symington L. Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing. Nature. 2008;455:770-4 pubmed publisher
    ..in eukaryotes, but several factors have been implicated, including the Mre11 complex (Mre11-Rad50-Xrs2/NBS1), Sae2/CtIP/Ctp1 and Exo1...

More Information

Publications68

  1. Mimitou E, Symington L. Ku prevents Exo1 and Sgs1-dependent resection of DNA ends in the absence of a functional MRX complex or Sae2. EMBO J. 2010;29:3358-69 pubmed publisher
    ..interplay between Ku, a central non-homologous end-joining component, and the Mre11-Rad50-Xrs2 (MRX) complex and Sae2, end-processing factors crucial for initiating 5'-3' resection of double-strand break (DSB) ends...
  2. Bonetti D, Clerici M, Manfrini N, Lucchini G, Longhese M. The MRX complex plays multiple functions in resection of Yku- and Rif2-protected DNA ends. PLoS ONE. 2010;5:e14142 pubmed publisher
    ..Resection of DNA ends, which is specifically inhibited during the G1 cell cycle phase, requires the MRX complex, Sae2, Sgs1 and Exo1...
  3. Donnianni R, Ferrari M, Lazzaro F, Clerici M, Tamilselvan Nachimuthu B, Plevani P, et al. Elevated levels of the polo kinase Cdc5 override the Mec1/ATR checkpoint in budding yeast by acting at different steps of the signaling pathway. PLoS Genet. 2010;6:e1000763 pubmed publisher
    ..Finally, we provide evidence that Sae2, the functional ortholog of human CtIP, which regulates DSB processing and inhibits checkpoint signaling, is ..
  4. Deng C, Brown J, You D, Brown J. Multiple endonucleases function to repair covalent topoisomerase I complexes in Saccharomyces cerevisiae. Genetics. 2005;170:591-600 pubmed
    ..in DSB repair and recombination, we identified four genes with known or implicated nuclease activity, SLX1, SLX4, SAE2, and RAD27, that were also important for protection against camptothecin...
  5. Ho H, Burgess S. Pch2 acts through Xrs2 and Tel1/ATM to modulate interhomolog bias and checkpoint function during meiosis. PLoS Genet. 2011;7:e1002351 pubmed publisher
    ..In addition, Xrs2, like Pch2, is required for checkpoint-mediated delay conferred by the failure to synapse chromosomes...
  6. Lengsfeld B, Rattray A, Bhaskara V, Ghirlando R, Paull T. Sae2 is an endonuclease that processes hairpin DNA cooperatively with the Mre11/Rad50/Xrs2 complex. Mol Cell. 2007;28:638-51 pubmed
    ..In budding yeast, genetic evidence suggests that the Sae2 protein is essential for the processing of hairpin DNA intermediates and meiotic double-strand breaks by Mre11/..
  7. Psakhye I, Jentsch S. Protein group modification and synergy in the SUMO pathway as exemplified in DNA repair. Cell. 2012;151:807-820 pubmed publisher
    ..We propose that SUMOylation may thus often target a protein group rather than individual proteins, whereas localized modification enzymes and highly specific triggers ensure specificity. ..
  8. McKee A, Kleckner N. A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2. Genetics. 1997;146:797-816 pubmed
    ..mutations of the DMC1 gene, nonnull mutations of RAD50 (rad50S), and mutations in three new genes designed SAE1, SAE2 and SAE3 (Sporulation in the Absence of Spo Eleven)...
  9. Lee K, Lee S. Saccharomyces cerevisiae Sae2- and Tel1-dependent single-strand DNA formation at DNA break promotes microhomology-mediated end joining. Genetics. 2007;176:2003-14 pubmed
    ..The mismatch repair proteins, Rad52 group genes, and Rad27 are dispensable for MMEJ. Sae2 and Tel1 promote MMEJ but inhibit NHEJ, likely by regulating Mre11-dependent ssDNA accumulation at DNA break...
  10. Kim H, Vijayakumar S, Reger M, Harrison J, Haber J, Weil C, et al. Functional interactions between Sae2 and the Mre11 complex. Genetics. 2008;178:711-23 pubmed publisher
    ..b>Sae2 deficiency has opposing effects on the Mre11 complex...
  11. Clerici M, Mantiero D, Lucchini G, Longhese M. The Saccharomyces cerevisiae Sae2 protein promotes resection and bridging of double strand break ends. J Biol Chem. 2005;280:38631-8 pubmed
    ..The function of the Saccharomyces cerevisiae Sae2 protein, which is known to work together with the MRX complex in meiotic DSB processing and in specific mitotic DSB ..
  12. Fu Q, Chow J, Bernstein K, Makharashvili N, Arora S, Lee C, et al. Phosphorylation-regulated transitions in an oligomeric state control the activity of the Sae2 DNA repair enzyme. Mol Cell Biol. 2014;34:778-93 pubmed publisher
    ..Here we investigated the DNA damage-induced oligomeric transitions of the Sae2 protein, an important enzyme in the initiation of DNA double-strand break repair...
  13. Foster S, Balestrini A, Petrini J. Functional interplay of the Mre11 nuclease and Ku in the response to replication-associated DNA damage. Mol Cell Biol. 2011;31:4379-89 pubmed publisher
    ..suppressed the camptothecin (CPT) and methyl methanesulfonate (MMS) sensitivity of nuclease-deficient mre11-3 and sae2? mutants in an Exo1-dependent manner...
  14. Bernstein K, Mimitou E, Mihalevic M, Chen H, Sunjaveric I, Symington L, et al. Resection activity of the Sgs1 helicase alters the affinity of DNA ends for homologous recombination proteins in Saccharomyces cerevisiae. Genetics. 2013;195:1241-51 pubmed publisher
    ..Compromising Sgs1 resection function in the absence of the Sae2 nuclease causes slow growth, which is alleviated by making the DNA ends accessible to Exo1 nuclease...
  15. Saini N, Zhang Y, Nishida Y, Sheng Z, Choudhury S, Mieczkowski P, et al. Fragile DNA motifs trigger mutagenesis at distant chromosomal loci in saccharomyces cerevisiae. PLoS Genet. 2013;9:e1003551 pubmed publisher
    ..Mutagenesis induced by inverted repeats also required Sae2 which opens hairpin-capped breaks and initiates end resection...
  16. Deng S, Yin Y, Petes T, Symington L. Mre11-Sae2 and RPA Collaborate to Prevent Palindromic Gene Amplification. Mol Cell. 2015;60:500-8 pubmed publisher
    ..of large palindromic duplications, the major class of chromosomal rearrangements recovered from yeast cells lacking Sae2 or the Mre11 nuclease...
  17. Ghodke I, Muniyappa K. Genetic and biochemical evidences reveal novel insights into the mechanism underlying Saccharomyces cerevisiae Sae2-mediated abrogation of DNA replication stress. J Biosci. 2016;41:615-641 pubmed
    ..Another protein linked to DSB repair is Sae2, which regulates MRX persistence at DSBs...
  18. Reginato G, Cannavo E, Cejka P. Physiological protein blocks direct the Mre11-Rad50-Xrs2 and Sae2 nuclease complex to initiate DNA end resection. Genes Dev. 2017;31:2325-2330 pubmed publisher
    ..homologous recombination is initiated by DNA end resection, which is commenced by the Mre11-Rad50-Xrs2 complex and Sae2 in yeast...
  19. Bantele S, Ferreira P, Gritenaite D, Boos D, Pfander B. Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection. elife. 2017;6: pubmed publisher
    ..Notably, artificial targeting of Fun30 to DSBs is sufficient to bypass the cell cycle regulation of long-range resection, indicating that chromatin remodelling during resection is underlying DSB repair pathway choice. ..
  20. Ballew B, Lundblad V. Multiple genetic pathways regulate replicative senescence in telomerase-deficient yeast. Aging Cell. 2013;12:719-27 pubmed publisher
    ..Moreover, defects in several additional proteins implicated in DSB repair (Rif1 and Sae2) confer only transient effects during early or late stages of replicative senescence, respectively, further ..
  21. Viscardi V, Baroni E, Romano M, Lucchini G, Longhese M. Sudden telomere lengthening triggers a Rad53-dependent checkpoint in Saccharomyces cerevisiae. Mol Biol Cell. 2003;14:3126-43 pubmed
    ..and checkpoint inactivation under galactose-induced conditions are accelerated by high levels of either the Sae2 protein, involved in double-strand breaks processing, or the negative telomere length regulator Rif2...
  22. Colosio A, Frattini C, Pellicanò G, Villa Hernández S, Bermejo R. Nucleolytic processing of aberrant replication intermediates by an Exo1-Dna2-Sae2 axis counteracts fork collapse-driven chromosome instability. Nucleic Acids Res. 2016;44:10676-10690 pubmed
    ..Genetic analyses demonstrated a functional interplay of Exo1 with Mus81, Dna2 and Sae2 nucleases in promoting cell survival following replication stress, suggestive of concerted nucleolytic processing ..
  23. Ferrari M, Dibitetto D, De Gregorio G, Eapen V, Rawal C, Lazzaro F, et al. Functional interplay between the 53BP1-ortholog Rad9 and the Mre11 complex regulates resection, end-tethering and repair of a double-strand break. PLoS Genet. 2015;11:e1004928 pubmed publisher
    The Mre11-Rad50-Xrs2 nuclease complex, together with Sae2, initiates the 5'-to-3' resection of Double-Strand DNA Breaks (DSBs)...
  24. Budd M, Campbell J. Interplay of Mre11 nuclease with Dna2 plus Sgs1 in Rad51-dependent recombinational repair. PLoS ONE. 2009;4:e4267 pubmed publisher
    ..We further show that sgs1Delta mre11-H125N, but not sgs1Delta, is very sensitive to IR, implicating the Sgs1 helicase in the Dna2-mediated pathway. ..
  25. Hohl M, Kochańczyk T, Tous C, Aguilera A, Krężel A, Petrini J. Interdependence of the rad50 hook and globular domain functions. Mol Cell. 2015;57:479-91 pubmed publisher
    ..We propose that transmission of spatial information in this manner underlies the regulation of Mre11 complex functions. ..
  26. Chen H, Donnianni R, Handa N, Deng S, Oh J, Timashev L, et al. Sae2 promotes DNA damage resistance by removing the Mre11-Rad50-Xrs2 complex from DNA and attenuating Rad53 signaling. Proc Natl Acad Sci U S A. 2015;112:E1880-7 pubmed publisher
    ..Saccharomyces cerevisiae Sae2 can function with MRX to initiate 5'-3' end resection and also plays an important role in attenuation of DNA damage ..
  27. Martina M, Bonetti D, Villa M, Lucchini G, Longhese M. Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection. EMBO Rep. 2014;15:695-704 pubmed publisher
    ..Here, we show that Saccharomyces cerevisiae Rif1 supports cell survival to DNA lesions in the absence of MRX or Sae2. Furthermore, it contributes to the nucleolytic processing (resection) of DSBs...
  28. Costelloe T, Louge R, Tomimatsu N, Mukherjee B, Martini E, Khadaroo B, et al. The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection. Nature. 2012;489:581-4 pubmed publisher
    ..In yeast, the Mre11-Rad50-Xrs2 complex (Xrs2 is known as NBN or NBS1 in humans) and Sae2 (known as RBBP8 or CTIP in humans) initiate end resection, whereas long-range resection depends on the exonuclease ..
  29. Keogh M, Kim J, Downey M, Fillingham J, Chowdhury D, Harrison J, et al. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature. 2006;439:497-501 pubmed
    ..The dephosphorylation of gammaH2AX by the HTP-C is necessary for efficient recovery from the DNA damage checkpoint. ..
  30. Iwasaki D, Hayashihara K, Shima H, Higashide M, Terasawa M, Gasser S, et al. The MRX Complex Ensures NHEJ Fidelity through Multiple Pathways Including Xrs2-FHA-Dependent Tel1 Activation. PLoS Genet. 2016;12:e1005942 pubmed publisher
    ..Thus, the Xrs2 FHA domain and Tel1 kinase work in a coordinated manner to maintain DSB repair fidelity. ..
  31. Andres S, Williams R. CtIP/Ctp1/Sae2, molecular form fit for function. DNA Repair (Amst). 2017;56:109-117 pubmed publisher
    Vertebrate CtIP, and its fission yeast (Ctp1), budding yeast (Sae2) and plant (Com1) orthologs have emerged as key regulatory molecules in cellular responses to DNA double strand breaks (DSBs)...
  32. Terasawa M, Ogawa T, Tsukamoto Y, Ogawa H. Sae2p phosphorylation is crucial for cooperation with Mre11p for resection of DNA double-strand break ends during meiotic recombination in Saccharomyces cerevisiae. Genes Genet Syst. 2008;83:209-17 pubmed
    ..These results indicate that phosphorylation of Sae2p is required to initiate resection and to improve the efficiency of resection through cooperation with the Mre11-Rad50-Xrs2 complex. ..
  33. Cejka P. DNA End Resection: Nucleases Team Up with the Right Partners to Initiate Homologous Recombination. J Biol Chem. 2015;290:22931-8 pubmed publisher
    ..of DNA end resection in Saccharomyces cerevisiae, which includes short-range resection by Mre11-Rad50-Xrs2 and Sae2, as well as processive long-range resection by Sgs1-Dna2 or Exo1 pathways...
  34. Oh J, Al Zain A, Cannavo E, Cejka P, Symington L. Xrs2 Dependent and Independent Functions of the Mre11-Rad50 Complex. Mol Cell. 2016;64:405-415 pubmed publisher
    ..Although Xrs2 physically interacts with Sae2, we found that end resection in its absence remains Sae2 dependent in vivo and in vitro...
  35. Guénolé A, Srivas R, Vreeken K, Wang Z, Wang S, Krogan N, et al. Dissection of DNA damage responses using multiconditional genetic interaction maps. Mol Cell. 2013;49:346-58 pubmed publisher
    ..Our multiconditional genetic interaction map provides a unique resource that identifies agent-specific and general DNA damage response pathways. ..
  36. Bonetti D, Villa M, Gobbini E, Cassani C, Tedeschi G, Longhese M. Escape of Sgs1 from Rad9 inhibition reduces the requirement for Sae2 and functional MRX in DNA end resection. EMBO Rep. 2015;16:351-61 pubmed publisher
    ..In Saccharomyces cerevisiae, the MRX complex and Sae2 are involved in the onset of DSB resection, whereas extensive resection requires Exo1 and the concerted action of ..
  37. Liang J, Suhandynata R, Zhou H. Phosphorylation of Sae2 Mediates Forkhead-associated (FHA) Domain-specific Interaction and Regulates Its DNA Repair Function. J Biol Chem. 2015;290:10751-63 pubmed publisher
    Saccharomyces cerevisiae Sae2 and its ortholog CtIP in higher eukaryotes have a conserved role in the initial processing of DNA lesions and influencing their subsequent repair pathways...
  38. Ball L, Hanna M, Lambrecht A, Mitchell B, Ziola B, Cobb J, et al. The Mre11-Rad50-Xrs2 complex is required for yeast DNA postreplication repair. PLoS ONE. 2014;9:e109292 pubmed publisher
    ..Further genetic analyses indicated that two other nucleases involved in double-strand end resection, Sae2 and Exo1, are also variably required for efficient lesion bypass...
  39. Arora S, Deshpande R, Budd M, Campbell J, Revere A, Zhang X, et al. Genetic Separation of Sae2 Nuclease Activity from Mre11 Nuclease Functions in Budding Yeast. Mol Cell Biol. 2017;37: pubmed publisher
    b>Sae2 promotes the repair of DNA double-strand breaks in Saccharomyces cerevisiae The role of Sae2 is linked to the Mre11/Rad50/Xrs2 (MRX) complex, which is important for the processing of DNA ends into single-stranded substrates ..
  40. Casper A, Greenwell P, Tang W, Petes T. Chromosome aberrations resulting from double-strand DNA breaks at a naturally occurring yeast fragile site composed of inverted ty elements are independent of Mre11p and Sae2p. Genetics. 2009;183:423-39, 1SI-26SI pubmed publisher
    ..Also, repair of DSBs by homologous recombination is efficient in mre11 and sae2 mutants...
  41. Dibitetto D, Ferrari M, Rawal C, Balint A, Kim T, Zhang Z, et al. Slx4 and Rtt107 control checkpoint signalling and DNA resection at double-strand breaks. Nucleic Acids Res. 2016;44:669-82 pubmed publisher
    ..Importantly, in slx4Δ sae2Δ double mutant cells these phenotypes are exacerbated, causing a severe Rad9-dependent defect in DSB repair...
  42. Refolio E, Cavero S, Marcon E, Freire R, San Segundo P. The Ddc2/ATRIP checkpoint protein monitors meiotic recombination intermediates. J Cell Sci. 2011;124:2488-500 pubmed publisher
    ..Thus, our results point to an evolutionary conserved role for Ddc2/ATRIP in monitoring meiotic chromosome metabolism. ..
  43. Ghodke I, Muniyappa K. Processing of DNA double-stranded breaks and intermediates of recombination and repair by Saccharomyces cerevisiae Mre11 and its stimulation by Rad50, Xrs2, and Sae2 proteins. J Biol Chem. 2013;288:11273-86 pubmed publisher
    ..Significantly, Rad50, Xrs2, and Sae2 potentiate the DNA unwinding activity of Mre11, thus underscoring functional interaction among the components of ..
  44. Uanschou C, Siwiec T, Pedrosa Harand A, Kerzendorfer C, Sanchez Moran E, Novatchkova M, et al. A novel plant gene essential for meiosis is related to the human CtIP and the yeast COM1/SAE2 gene. EMBO J. 2007;26:5061-70 pubmed
    ..In Saccharomyces cerevisiae, Rad50, Mre11 and Com1/Sae2 are essential to process an intermediate of the cleavage reaction consisting of Spo11 covalently linked to the 5' ..
  45. Hardy J, Churikov D, Géli V, Simon M. Sgs1 and Sae2 promote telomere replication by limiting accumulation of ssDNA. Nat Commun. 2014;5:5004 pubmed publisher
    In budding yeast, DNA ends are processed by the consecutive action of MRX/Sae2 and two redundant pathways dependent on Sgs1/Dna2 and Exo1, and this processing is counteracted by Ku heterodimer...
  46. Hang L, Lopez C, Liu X, Williams J, Chung I, Wei L, et al. Regulation of Ku-DNA association by Yku70 C-terminal tail and SUMO modification. J Biol Chem. 2014;289:10308-17 pubmed publisher
    ..These results suggest a model whereby Yku70 sumoylation upon DNA association strengthens Ku-DNA interaction to promote multiple functions of Ku. ..
  47. Garcia V, Gray S, Allison R, Cooper T, Neale M. Tel1(ATM)-mediated interference suppresses clustered meiotic double-strand-break formation. Nature. 2015;520:114-8 pubmed publisher
    ..Such collective negative regulation will help to ensure that recombination events are dispersed evenly and arranged optimally for genetic exchange and efficient chromosome segregation. ..
  48. Fukunaga K, Kwon Y, Sung P, Sugimoto K. Activation of protein kinase Tel1 through recognition of protein-bound DNA ends. Mol Cell Biol. 2011;31:1959-71 pubmed publisher
    ..In vivo, Tel1 activation is enhanced in sae2? or mre11-3 mutants after camptothecin treatment; both of these mutants are defective in the removal of ..
  49. Clerici M, Trovesi C, Galbiati A, Lucchini G, Longhese M. Mec1/ATR regulates the generation of single-stranded DNA that attenuates Tel1/ATM signaling at DNA ends. EMBO J. 2014;33:198-216 pubmed publisher
    ..Thus, Mec1 regulates the generation of ssDNA at DSBs, and this control is important to coordinate Mec1 and Tel1 signaling activities at these breaks. ..
  50. Debrauwere H, Loeillet S, Lin W, Lopes J, Nicolas A. Links between replication and recombination in Saccharomyces cerevisiae: a hypersensitive requirement for homologous recombination in the absence of Rad27 activity. Proc Natl Acad Sci U S A. 2001;98:8263-9 pubmed
    ..that confer weaker phenotypes in mitotic than in meiotic cells (rad50S, mre11s) and additional gene deletions (com1/sae2, srs2) exhibit synthetic lethality with rad27 Delta and that rad59 Delta exhibits synergistic effects with rad27 ..
  51. Sarangi P, Steinacher R, Altmannova V, Fu Q, Paull T, Krejci L, et al. Sumoylation influences DNA break repair partly by increasing the solubility of a conserved end resection protein. PLoS Genet. 2015;11:e1004899 pubmed publisher
    ..Here, we show that sumoylation regulates double-strand break repair partly by modifying the end resection factor Sae2. This modification is conserved from yeast to humans, and is induced by DNA damage...
  52. Woltering D, Baumgartner B, Bagchi S, Larkin B, Loidl J, de los Santos T, et al. Meiotic segregation, synapsis, and recombination checkpoint functions require physical interaction between the chromosomal proteins Red1p and Hop1p. Mol Cell Biol. 2000;20:6646-58 pubmed
    ..Furthermore, red1-K348E suppresses the sae2/com1 defects in meiotic progression and sporulation, indicating a previously unknown role for HOP1 in the meiotic ..
  53. Manfrini N, Guerini I, Citterio A, Lucchini G, Longhese M. Processing of meiotic DNA double strand breaks requires cyclin-dependent kinase and multiple nucleases. J Biol Chem. 2010;285:11628-37 pubmed publisher
    ..In Saccharomyces cerevisiae, the Sae2 protein and the Mre11-Rad50-Xrs2 complex are necessary to remove the covalently attached Spo11 protein from the DNA ..
  54. McKee A, Kleckner N. Mutations in Saccharomyces cerevisiae that block meiotic prophase chromosome metabolism and confer cell cycle arrest at pachytene identify two new meiosis-specific genes SAE1 and SAE3. Genetics. 1997;146:817-34 pubmed
    ..SAE1 encodes a 208-residue protein homologous to vertebrate mRNA cap-binding protein 20. SAE3 corresponds to a meiosis-specific RNA encoding an unusually short open reading frame of 50 codons. ..
  55. Puddu F, Oelschlaegel T, Guerini I, Geisler N, Niu H, Herzog M, et al. Synthetic viability genomic screening defines Sae2 function in DNA repair. EMBO J. 2015;34:1509-22 pubmed publisher
    ..During meiosis, yeast Sae2 cooperates with the nuclease Mre11 to remove covalently bound Spo11 from DSB termini, allowing resection and HR to ..
  56. Muñoz Galvan S, López Saavedra A, Jackson S, Huertas P, Cortes Ledesma F, Aguilera A. Competing roles of DNA end resection and non-homologous end joining functions in the repair of replication-born double-strand breaks by sister-chromatid recombination. Nucleic Acids Res. 2013;41:1669-83 pubmed publisher
    ..end resection, which in Saccharomyces cerevisiae is controlled by the MRX complex and the Sgs1 DNA helicase or the Sae2 and Exo1 nucleases...
  57. Gobbini E, Villa M, Gnugnoli M, Menin L, Clerici M, Longhese M. Sae2 Function at DNA Double-Strand Breaks Is Bypassed by Dampening Tel1 or Rad53 Activity. PLoS Genet. 2015;11:e1005685 pubmed publisher
    The MRX complex together with Sae2 initiates resection of DNA double-strand breaks (DSBs) to generate single-stranded DNA (ssDNA) that triggers homologous recombination...
  58. Putnam C, Pallis K, Hayes T, Kolodner R. DNA repair pathway selection caused by defects in TEL1, SAE2, and de novo telomere addition generates specific chromosomal rearrangement signatures. PLoS Genet. 2014;10:e1004277 pubmed publisher
    ..CAN1/URA3 cassette on chrV L while retaining a chrV L telomeric hph marker was significantly higher in tel1?, sae2?, rad53? sml1?, and mrc1? tof1? mutants...
  59. Farmer S, Hong E, Leung W, Argunhan B, Terentyev Y, Humphryes N, et al. Budding yeast Pch2, a widely conserved meiotic protein, is involved in the initiation of meiotic recombination. PLoS ONE. 2012;7:e39724 pubmed publisher
    ..The reduction of DSB formation caused by the pch2 mutation is most prominent in the sae2 mutant background, whereas the impact remains mild in the rad51 dmc1 double mutant background...