RSP5

Summary

Gene Symbol: RSP5
Description: NEDD4 family E3 ubiquitin-protein ligase
Alias: MDP1, MUT2, NPI1, SMM1, UBY1, NEDD4 family E3 ubiquitin-protein ligase
Species: Saccharomyces cerevisiae S288c
Products:     RSP5

Top Publications

  1. Wang G, Yang J, Huibregtse J. Functional domains of the Rsp5 ubiquitin-protein ligase. Mol Cell Biol. 1999;19:342-52 pubmed
    b>RSP5, an essential gene of Saccharomyces cerevisiae, encodes a hect domain E3 ubiquitin-protein ligase...
  2. Morvan J, Froissard M, Haguenauer Tsapis R, Urban Grimal D. The ubiquitin ligase Rsp5p is required for modification and sorting of membrane proteins into multivesicular bodies. Traffic. 2004;5:383-92 pubmed
    ..In cells with low levels of Rsp5p (npi1 mutant cells), vacuolar hydrolases do not reach the vacuolar lumen and are instead missorted to the vacuolar ..
  3. Wang S, Thibault G, Ng D. Routing misfolded proteins through the multivesicular body (MVB) pathway protects against proteotoxicity. J Biol Chem. 2011;286:29376-87 pubmed publisher
    ..Eliminating the MVB sorting signal diverted molecules to the vacuolar limiting membrane, resulting in the generation of toxic by-products. These data demonstrate a new role of the MVB pathway in protein quality control. ..
  4. Stawiecka Mirota M, Pokrzywa W, Morvan J, Zoladek T, Haguenauer Tsapis R, Urban Grimal D, et al. Targeting of Sna3p to the endosomal pathway depends on its interaction with Rsp5p and multivesicular body sorting on its ubiquitylation. Traffic. 2007;8:1280-96 pubmed
    ..Mutation of either the Sna3p PPXY motif or the Rsp5p WW3 domain or reduction in the amounts of Rsp5 results in the mistargeting of Sna3p to multiple mobile vesicles and prevents its sorting to the endosomal pathway...
  5. Hettema E, Valdez Taubas J, Pelham H. Bsd2 binds the ubiquitin ligase Rsp5 and mediates the ubiquitination of transmembrane proteins. EMBO J. 2004;23:1279-88 pubmed
    ..Bsd2 is a three-TMD protein with a PPXY motif that binds the HECT domain ubiquitin ligase Rsp5. It can thus act as a specific adaptor linking Rsp5 to its substrates...
  6. Kee Y, Muñoz W, Lyon N, Huibregtse J. The deubiquitinating enzyme Ubp2 modulates Rsp5-dependent Lys63-linked polyubiquitin conjugates in Saccharomyces cerevisiae. J Biol Chem. 2006;281:36724-31 pubmed
    ..b>Rsp5 is a HECT (homologous to E6AP C terminus) ubiquitin ligase involved in numerous processes, and an associated ..
  7. Ren J, Kee Y, Huibregtse J, Piper R. Hse1, a component of the yeast Hrs-STAM ubiquitin-sorting complex, associates with ubiquitin peptidases and a ligase to control sorting efficiency into multivesicular bodies. Mol Biol Cell. 2007;18:324-35 pubmed
    ..We show that the endosomal Hse1-Vps27 sorting receptor binds to ubiquitin peptidases and the ubiquitin ligase Rsp5. Hse1 is linked to Rsp5 directly via a PY element within its C-terminus and through a novel protein Hua1, which ..
  8. Lee J, Oestreich A, Payne J, Gunawan M, Norgan A, Katzmann D. The HECT domain of the ubiquitin ligase Rsp5 contributes to substrate recognition. J Biol Chem. 2009;284:32126-37 pubmed publisher
    ..We have investigated substrate recognition by the Nedd4 ortholog Rsp5 as a model for understanding ligase-substrate interactions...
  9. Novoselova T, Zahira K, Rose R, Sullivan J. Bul proteins, a nonredundant, antagonistic family of ubiquitin ligase regulatory proteins. Eukaryot Cell. 2012;11:463-70 pubmed publisher

More Information

Publications69

  1. Springael J, Andre B. Nitrogen-regulated ubiquitination of the Gap1 permease of Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1253-63 pubmed
    ..rapid inactivation and degradation of the general amino acid permease Gap1 through a process requiring the Npi1/Rsp5 ubiquitin (Ub) ligase...
  2. Gwizdek C, Hobeika M, Kus B, Ossareh Nazari B, Dargemont C, Rodriguez M. The mRNA nuclear export factor Hpr1 is regulated by Rsp5-mediated ubiquitylation. J Biol Chem. 2005;280:13401-5 pubmed
    ..Thus, Hpr1p represents the first nuclear export factor regulated by ubiquitylation, strongly suggesting that this post-translational modification participates in the coordination of transcription and mRNA export processes. ..
  3. Huibregtse J, Yang J, Beaudenon S. The large subunit of RNA polymerase II is a substrate of the Rsp5 ubiquitin-protein ligase. Proc Natl Acad Sci U S A. 1997;94:3656-61 pubmed
    ..A biochemical approach was taken to identify substrates of Rsp5, an essential hect (homologous to E6-AP carboxyl terminus) E3 of Saccharomyces cerevisiae...
  4. Dunn R, Hicke L. Domains of the Rsp5 ubiquitin-protein ligase required for receptor-mediated and fluid-phase endocytosis. Mol Biol Cell. 2001;12:421-35 pubmed
    ..Temperature-sensitive rsp5 mutants were unable to ubiquitinate or to internalize Ste2p at the nonpermissive temperature...
  5. Helliwell S, Losko S, Kaiser C. Components of a ubiquitin ligase complex specify polyubiquitination and intracellular trafficking of the general amino acid permease. J Cell Biol. 2001;153:649-62 pubmed
    ..Both a rsp5-1 mutant and a COOH-terminal truncation of Gap1p behave as bul1Delta bul2Delta, causing constitutive delivery of ..
  6. Somesh B, Reid J, Liu W, Søgaard T, Erdjument Bromage H, Tempst P, et al. Multiple mechanisms confining RNA polymerase II ubiquitylation to polymerases undergoing transcriptional arrest. Cell. 2005;121:913-23 pubmed
    ..These results identify several mechanisms that confine ubiquitylation of RNAPII to the forms of the enzyme that arrest during elongation. ..
  7. Andoh T, Hirata Y, Kikuchi A. PY motifs of Rod1 are required for binding to Rsp5 and for drug resistance. FEBS Lett. 2002;525:131-4 pubmed
    ..Furthermore, these two proteins have PY-motifs, and bound to Rsp5, a hect-type ubiquitin ligase...
  8. French M, Kretzmann B, Hicke L. Regulation of the RSP5 ubiquitin ligase by an intrinsic ubiquitin-binding site. J Biol Chem. 2009;284:12071-9 pubmed publisher
    b>Rsp5 is a homologous to E6AP C terminus (HECT) ubiquitin ligase (E3) that controls many different cellular processes in budding yeast...
  9. Kee Y, Lyon N, Huibregtse J. The Rsp5 ubiquitin ligase is coupled to and antagonized by the Ubp2 deubiquitinating enzyme. EMBO J. 2005;24:2414-24 pubmed
    Saccharomyces cerevisiae Rsp5 is an essential HECT ubiquitin ligase involved in several biological processes. To gain further insight into regulation of this enzyme, we identified proteins that copurified with epitope-tagged Rsp5...
  10. Stimpson H, Lewis M, Pelham H. Transferrin receptor-like proteins control the degradation of a yeast metal transporter. EMBO J. 2006;25:662-72 pubmed
    ..Degradation requires Bsd2, a membrane protein with a PPxY motif that recruits the ubiquitin ligase Rsp5, and which has a role in the quality control of membrane proteins, that expose hydrophilic residues to the lipid ..
  11. Hatakeyama R, Kamiya M, Takahara T, Maeda T. Endocytosis of the aspartic acid/glutamic acid transporter Dip5 is triggered by substrate-dependent recruitment of the Rsp5 ubiquitin ligase via the arrestin-like protein Aly2. Mol Cell Biol. 2010;30:5598-607 pubmed publisher
    ..cerevisiae, endocytosis is triggered by ubiquitination of transporters catalyzed by the E3 ubiquitin ligase Rsp5. However, how the ubiquitination is accelerated under certain conditions remains obscure...
  12. Fisk H, Yaffe M. A role for ubiquitination in mitochondrial inheritance in Saccharomyces cerevisiae. J Cell Biol. 1999;145:1199-208 pubmed
    The smm1 mutation suppresses defects in mitochondrial distribution and morphology caused by the mdm1-252 mutation in the yeast Saccharomyces cerevisiae...
  13. Nikko E, Sullivan J, Pelham H. Arrestin-like proteins mediate ubiquitination and endocytosis of the yeast metal transporter Smf1. EMBO Rep. 2008;9:1216-21 pubmed publisher
    ..manganese transporter Smf1 is endocytosed when cells are exposed to cadmium ions, that this endocytosis depends on Rsp5-dependent ubiquitination of specific lysines and that it also requires phosphorylation at nearby sites...
  14. Katzmann D, Sarkar S, Chu T, Audhya A, Emr S. Multivesicular body sorting: ubiquitin ligase Rsp5 is required for the modification and sorting of carboxypeptidase S. Mol Biol Cell. 2004;15:468-80 pubmed
    ..This selection identified a point mutation in ubiquitin ligase Rsp5 (Rsp5-326)...
  15. Yashiroda H, Oguchi T, Yasuda Y, Toh e A, Kikuchi Y. Bul1, a new protein that binds to the Rsp5 ubiquitin ligase in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:3255-63 pubmed
    ..By using the two-hybrid system, we further isolated RSP5 (reverses Spt- phenotype), which encodes a hect (homologous to E6-AP C terminus) domain, as a gene encoding a ..
  16. Lam M, Urban Grimal D, Bugnicourt A, Greenblatt J, Haguenauer Tsapis R, Emili A. Interaction of the deubiquitinating enzyme Ubp2 and the e3 ligase Rsp5 is required for transporter/receptor sorting in the multivesicular body pathway. PLoS ONE. 2009;4:e4259 pubmed publisher
    ..we (this study) and others established that affinity purified Ubp2 interacts stably with the E3 ubiquitin ligase Rsp5 and the (ubiquitin associated) UBA domain containing protein Rup1...
  17. Dunn R, Klos D, Adler A, Hicke L. The C2 domain of the Rsp5 ubiquitin ligase binds membrane phosphoinositides and directs ubiquitination of endosomal cargo. J Cell Biol. 2004;165:135-44 pubmed
    ..Here, we investigate the role of the yeast Nedd4 homologue, Rsp5, in protein sorting into vesicles that bud into the multivesicular endosome (MVE) en route to the vacuole...
  18. Paiva S, Vieira N, Nondier I, Haguenauer Tsapis R, Casal M, Urban Grimal D. Glucose-induced ubiquitylation and endocytosis of the yeast Jen1 transporter: role of lysine 63-linked ubiquitin chains. J Biol Chem. 2009;284:19228-36 pubmed publisher
    ..We show here that casein kinase 1-dependent phosphorylation and HECT-ubiquitin ligase Rsp5-dependent ubiquitylation are required for Jen1 endocytosis...
  19. Kaminska J, Spiess M, Stawiecka Mirota M, Monkaityte R, Haguenauer Tsapis R, Urban Grimal D, et al. Yeast Rsp5 ubiquitin ligase affects the actin cytoskeleton in vivo and in vitro. Eur J Cell Biol. 2011;90:1016-28 pubmed publisher
    Yeast Rsp5 ubiquitin ligase is involved in several cellular processes, including endocytosis. Actin patches are sites of endocytosis, a process involving actin assembly and disassembly...
  20. Kaliszewski P, Ferreira T, Gajewska B, Szkopinska A, Berges T, Zoładek T. Enhanced levels of Pis1p (phosphatidylinositol synthase) improve the growth of Saccharomyces cerevisiae cells deficient in Rsp5 ubiquitin ligase. Biochem J. 2006;395:173-81 pubmed
    The Rsp5 ubiquitin ligase plays a role in many cellular processes including the biosynthesis of unsaturated fatty acids...
  21. Krsmanovic T, Kölling R. The HECT E3 ubiquitin ligase Rsp5 is important for ubiquitin homeostasis in yeast. FEBS Lett. 2004;577:215-9 pubmed
    The HECT E3 ubiquitin ligase Rsp5, a yeast member of the Nedd4 family, has been implicated in many different aspects of cell physiology. Here, we present evidence that Rsp5 function is important for ubiquitin homeostasis...
  22. Kraft C, Peter M. Is the Rsp5 ubiquitin ligase involved in the regulation of ribophagy?. Autophagy. 2008;4:838-40 pubmed
    ..Here we present data on a potential role of the ubiquitin ligase Rsp5 as a positive regulator of ribophagy, and discuss the possible involvement of ubiquitin as a signaling molecule in ..
  23. Zoladek T, Tobiasz A, Vaduva G, Boguta M, Martin N, Hopper A. MDP1, a Saccharomyces cerevisiae gene involved in mitochondrial/cytoplasmic protein distribution, is identical to the ubiquitin-protein ligase gene RSP5. Genetics. 1997;145:595-603 pubmed
    ..MDP1 is identical to RSP5, which encodes ubiquitin-protein ligase, and mdp1 mutations are suppressed by high copy expression of ubiquitin...
  24. Kus B, Gajadhar A, Stanger K, Cho R, Sun W, Rouleau N, et al. A high throughput screen to identify substrates for the ubiquitin ligase Rsp5. J Biol Chem. 2005;280:29470-8 pubmed
    ..proteins for ubiquitination, and we identified previously reported and novel substrates of the yeast E3 ligase Rsp5. The relevance of these substrates was confirmed in vivo by showing that a number of them interact genetically with ..
  25. Leon S, Erpapazoglou Z, Haguenauer Tsapis R. Ear1p and Ssh4p are new adaptors of the ubiquitin ligase Rsp5p for cargo ubiquitylation and sorting at multivesicular bodies. Mol Biol Cell. 2008;19:2379-88 pubmed publisher
    ..Therefore, Ear1p/Ssh4p recruit Rsp5p and assist it in its function at MVBs by directing the ubiquitylation of specific cargoes. ..
  26. Ossareh Nazari B, Cohen M, Dargemont C. The Rsp5 ubiquitin ligase and the AAA-ATPase Cdc48 control the ubiquitin-mediated degradation of the COPII component Sec23. Exp Cell Res. 2010;316:3351-7 pubmed publisher
    ..Here we show that ubiquitylation of Sec23 is controlled by the Rsp5 ubiquitin ligase both in vivo and in vitro...
  27. Strochlic T, Schmiedekamp B, Lee J, Katzmann D, Burd C. Opposing activities of the Snx3-retromer complex and ESCRT proteins mediate regulated cargo sorting at a common endosome. Mol Biol Cell. 2008;19:4694-706 pubmed publisher
    ..In mutant cells lacking any component of the ESCRT protein-dependent MVB sorting machinery, the Rsp5 ubiquitin ligase, or in wild-type cells expressing Fet3-Ftr1 lacking cytosolic lysyl ubiquitin acceptor sites, Fet3-..
  28. Beaudenon S, Huacani M, Wang G, McDonnell D, Huibregtse J. Rsp5 ubiquitin-protein ligase mediates DNA damage-induced degradation of the large subunit of RNA polymerase II in Saccharomyces cerevisiae. Mol Cell Biol. 1999;19:6972-9 pubmed
    b>Rsp5 is an E3 ubiquitin-protein ligase of Saccharomyces cerevisiae that belongs to the hect domain family of E3 proteins. We have previously shown that Rsp5 binds and ubiquitinates the largest subunit of RNA polymerase II, Rpb1, in vitro...
  29. Lin C, MacGurn J, Chu T, Stefan C, Emr S. Arrestin-related ubiquitin-ligase adaptors regulate endocytosis and protein turnover at the cell surface. Cell. 2008;135:714-25 pubmed publisher
    ..These motifs are required for recruitment of the Rsp5/Nedd4-like ubiquitin ligase, which modifies the cargoes as well as the ARTs...
  30. Bhattacharya S, Shcherbik N, Vasilescu J, Smith J, Figeys D, Haines D. Identification of lysines within membrane-anchored Mga2p120 that are targets of Rsp5p ubiquitination and mediate mobilization of tethered Mga2p90. J Mol Biol. 2009;385:718-25 pubmed publisher
    ..We conclude that residues 980, 983, and 985 are targets of Rsp5p-induced polyubiquitination and mediate Cdc48p(Npl4p/Ufd1p)-dependent Mga2p90-Mga2p120 separation and Mga2p90 mobilization. ..
  31. McNatt M, McKittrick I, West M, Odorizzi G. Direct binding to Rsp5 mediates ubiquitin-independent sorting of Sna3 via the multivesicular body pathway. Mol Biol Cell. 2007;18:697-706 pubmed
    ..We show that Sna3 circumvents Ub-mediated recognition by interacting directly with Rsp5, an E3 Ub ligase that catalyzes monoubiquitination of MVB vesicle cargoes...
  32. Kim H, Steffen A, Oldham M, Chen J, Huibregtse J. Structure and function of a HECT domain ubiquitin-binding site. EMBO Rep. 2011;12:334-41 pubmed publisher
    The Rsp5 ubiquitin ligase contains a non-covalent binding site for ubiquitin within the amino-terminal lobe (N-lobe) of the HECT domain, and the X-ray crystal structure of the HECT-ubiquitin complex has been determined...
  33. Stamenova S, Dunn R, Adler A, Hicke L. The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167. J Biol Chem. 2004;279:16017-25 pubmed
    ..This ubiquitin ligase, Rsp5, binds directly to both Sla1 and Rvs167...
  34. Sullivan J, Lewis M, Nikko E, Pelham H. Multiple interactions drive adaptor-mediated recruitment of the ubiquitin ligase rsp5 to membrane proteins in vivo and in vitro. Mol Biol Cell. 2007;18:2429-40 pubmed
    Recognition of membrane proteins by the Nedd4/Rsp5 ubiquitin ligase family is a critical step in their targeting to the multivesicular body pathway...
  35. Herrador A, Herranz S, Lara D, Vincent O. Recruitment of the ESCRT machinery to a putative seven-transmembrane-domain receptor is mediated by an arrestin-related protein. Mol Cell Biol. 2010;30:897-907 pubmed publisher
    ..We present evidence that Rim8 ubiquitination is dependent on the Rsp5 E3 ubiquitin ligase and triggered upon binding of Vps23 UEV to both the SXP motif and ubiquitin, thus suggesting a ..
  36. Yashiroda H, Kaida D, Toh e A, Kikuchi Y. The PY-motif of Bul1 protein is essential for growth of Saccharomyces cerevisiae under various stress conditions. Gene. 1998;225:39-46 pubmed
    The previously identified BUL1 gene was found to encode a protein bound to Rsp5-ubiquitin ligase in budding yeast. We have identified the BUL2 gene as a functional homologue of BUL1...
  37. Harreman M, Taschner M, Sigurdsson S, Anindya R, Reid J, Somesh B, et al. Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation. Proc Natl Acad Sci U S A. 2009;106:20705-10 pubmed publisher
    ..The mechanism regulating proteolysis of RNAPII has been controversial since two distinct ubiquitin ligases (E3s), Rsp5 (and its human homologue NEDD4) and Elongin-Cullin complex, have both been shown to be required for its DNA-damage-..
  38. Chang A, Cheang S, Espanel X, Sudol M. Rsp5 WW domains interact directly with the carboxyl-terminal domain of RNA polymerase II. J Biol Chem. 2000;275:20562-71 pubmed
    b>RSP5 is an essential gene in Saccharomyces cerevisiae and was recently shown to form a physical and functional complex with RNA polymerase II (RNA pol II)...
  39. Kim H, Huibregtse J. Polyubiquitination by HECT E3s and the determinants of chain type specificity. Mol Cell Biol. 2009;29:3307-18 pubmed publisher
    ..catalyze the formation of K48-linked polyubiquitin chains, while others, including Saccharomyces cerevisiae Rsp5 and human Itch, preferentially catalyze the formation of K63-linked chains...
  40. Watson H, Bonifacino J. Direct binding to Rsp5p regulates ubiquitination-independent vacuolar transport of Sna3p. Mol Biol Cell. 2007;18:1781-9 pubmed
    ..Our data demonstrate that Sna3p follows a novel ubiquitination-independent, but Rsp5p-mediated, sorting pathway to the vacuole. ..
  41. Wu X, Chang A, Sudol M, Hanes S. Genetic interactions between the ESS1 prolyl-isomerase and the RSP5 ubiquitin ligase reveal opposing effects on RNA polymerase II function. Curr Genet. 2001;40:234-42 pubmed
    ..Interaction of these complexes with the CTD might be subject to regulation by proteins such as Ess1 and Rsp5. Ess1, a prolyl-isomerase, binds the CTD and is thought to play a positive role in pol II transcription by ..
  42. Oestreich A, Aboian M, Lee J, Azmi I, Payne J, Issaka R, et al. Characterization of multiple multivesicular body sorting determinants within Sna3: a role for the ubiquitin ligase Rsp5. Mol Biol Cell. 2007;18:707-20 pubmed
    ..This PPAY motif interacts with the WW domains of the ubiquitin ligase Rsp5, and mutations in either the WW or, surprisingly, the HECT domains of Rsp5 negatively impacted MVB targeting of ..
  43. Kaminska J, Gajewska B, Hopper A, Zoładek T. Rsp5p, a new link between the actin cytoskeleton and endocytosis in the yeast Saccharomyces cerevisiae. Mol Cell Biol. 2002;22:6946-8 pubmed
    ..We report the following. (i) There are genetic interactions between rsp5 and several mutant genes encoding actin cytoskeletal proteins...
  44. Lu J, Lin Y, Qian J, Tao S, Zhu J, Pickart C, et al. Functional dissection of a HECT ubiquitin E3 ligase. Mol Cell Proteomics. 2008;7:35-45 pubmed
    ..vivo biochemical analyses to identify and characterize eight novel in vivo substrates of the ubiquitinating enzyme Rsp5, a homolog of the human ubiquitin-ligating enzyme Nedd4, in yeast...
  45. Hoppe T, Matuschewski K, Rape M, Schlenker S, Ulrich H, Jentsch S. Activation of a membrane-bound transcription factor by regulated ubiquitin/proteasome-dependent processing. Cell. 2000;102:577-86 pubmed
    ..Intriguingly, proteasome-dependent processing of SPT23 is regulated by fatty acid pools, suggesting that the precursor itself or interacting partners are sensors of membrane composition or fluidity. ..
  46. Jarmoszewicz K, Łukasiak K, Riezman H, Kaminska J. Rsp5 ubiquitin ligase is required for protein trafficking in Saccharomyces cerevisiae COPI mutants. PLoS ONE. 2012;7:e39582 pubmed publisher
    ..Here we find that the ubiquitin ligase mutation, rsp5-1, has a negative effect that is additive with ret1-1 and sec28? mutations, in genes encoding ?- and ?-COP, ..
  47. Shcherbik N, Zoladek T, Nickels J, Haines D. Rsp5p is required for ER bound Mga2p120 polyubiquitination and release of the processed/tethered transactivator Mga2p90. Curr Biol. 2003;13:1227-33 pubmed
    ..Cells lacking Rsp5p function require oleic acid for growth, and Spt23p processing is suppressed in rsp5 Delta cells and in wild-type RSP5 cells upon expression of Rsp5p dominant-negative mutants...
  48. Auty R, Steen H, Myers L, Persinger J, Bartholomew B, Gygi S, et al. Purification of active TFIID from Saccharomyces cerevisiae. Extensive promoter contacts and co-activator function. J Biol Chem. 2004;279:49973-81 pubmed
    ..Analysis of the final elution by mass spectrometry confirms the presence of all the known TAFs and TBP, as well as Rsp5, Bul1, Ubp3, Bre5, Cka1, and Cka2...
  49. Attali I, Tobelaim W, Persaud A, Motamedchaboki K, Simpson Lavy K, Mashahreh B, et al. Ubiquitylation-dependent oligomerization regulates activity of Nedd4 ligases. EMBO J. 2017;36:425-440 pubmed publisher
    ..Similarly, electrophysiological data and TIRF microscopy show that NEDD4 unrestrained mutant constitutively downregulates the IKS channel, thus confirming the functional importance of E3-ligase autoinhibition. ..
  50. Kwapisz M, Cholbinski P, Hopper A, Rousset J, Zoladek T. Rsp5 ubiquitin ligase modulates translation accuracy in yeast Saccharomyces cerevisiae. RNA. 2005;11:1710-8 pubmed
    ..Recent studies indicate that ubiquitination also affects translation. Here, we show that the strain with the rsp5-13 mutation exhibits altered sensitivity to antibiotics and a slower rate of translation...
  51. Paul B, Montpetit B. Altered RNA processing and export lead to retention of mRNAs near transcription sites and nuclear pore complexes or within the nucleolus. Mol Biol Cell. 2016;27:2742-56 pubmed publisher
    ..These data show that alterations to various nuclear processes lead to the retention of mRNAs at discrete locations within the nucleus. ..
  52. Schwarz S, Rosa J, Scheffner M. Characterization of human hect domain family members and their interaction with UbcH5 and UbcH7. J Biol Chem. 1998;273:12148-54 pubmed
    ..Since E3s are thought to play a major role in substrate recognition, the presence of a large family of E3s should contribute to ensure the specificity and selectivity of ubiquitin-dependent proteolytic pathways. ..
  53. Crespo J, Helliwell S, Wiederkehr C, Demougin P, Fowler B, Primig M, et al. NPR1 kinase and RSP5-BUL1/2 ubiquitin ligase control GLN3-dependent transcription in Saccharomyces cerevisiae. J Biol Chem. 2004;279:37512-7 pubmed
    ..We also demonstrate that the E3/E4 ubiquitin-protein ligase proteins RSP5 and BUL1/2 are required for GLN3 activation under poor nitrogen conditions...
  54. Lu K, Psakhye I, Jentsch S. Autophagic clearance of polyQ proteins mediated by ubiquitin-Atg8 adaptors of the conserved CUET protein family. Cell. 2014;158:549-63 pubmed publisher
    ..Cue5 collaborates with Rsp5 ubiquitin ligase, and the corresponding yeast mutants accumulate aggregation-prone proteins and are vulnerable to ..
  55. Waite A, Shou W. Adaptation to a new environment allows cooperators to purge cheaters stochastically. Proc Natl Acad Sci U S A. 2012;109:19079-86 pubmed publisher
    ..We speculate that adaptation to changing environments may contribute to the persistence of cooperative systems before the appearance of more sophisticated mechanisms of cheater control. ..
  56. Li M, Rong Y, Chuang Y, Peng D, Emr S. Ubiquitin-dependent lysosomal membrane protein sorting and degradation. Mol Cell. 2015;57:467-78 pubmed publisher
    ..This selective degradation process requires a vacuole anchored ubiquitin ligase (VAcUL-1) complex composed of Rsp5 and Ssh4. We propose that after ubiquitination, Ypq1 is selectively sorted into an intermediate compartment...
  57. Alvaro C, Aindow A, Thorner J. Differential Phosphorylation Provides a Switch to Control How α-Arrestin Rod1 Down-regulates Mating Pheromone Response in Saccharomyces cerevisiae. Genetics. 2016;203:299-317 pubmed publisher
    ..association with an adaptor protein, the α-arrestin Rod1/Art4, which recruits the HECT-domain ubiquitin ligase Rsp5, allowing for ubiquitinylation of the C-terminal tail of the receptor and its engagement by the clathrin-dependent ..
  58. Soares L, Buratowski S. Yeast Swd2 is essential because of antagonism between Set1 histone methyltransferase complex and APT (associated with Pta1) termination factor. J Biol Chem. 2012;287:15219-31 pubmed publisher
    ..Although Swd2 is normally required for bringing APT to genes, deletion of SET1 restores both viability and APT recruitment to a strain lacking Swd2. We propose a model in which Swd2 is required for APT to overcome antagonism by COMPASS. ..
  59. Kamadurai H, Qiu Y, Deng A, Harrison J, MacDonald C, Actis M, et al. Mechanism of ubiquitin ligation and lysine prioritization by a HECT E3. elife. 2013;2:e00828 pubmed publisher
    ..DOI:http://dx.doi.org/10.7554/eLife.00828.001...
  60. Neumann S, Petfalski E, Brugger B, Grosshans H, Wieland F, Tollervey D, et al. Formation and nuclear export of tRNA, rRNA and mRNA is regulated by the ubiquitin ligase Rsp5p. EMBO Rep. 2003;4:1156-62 pubmed
    ..Here, we identify Rsp5p in a screen for tRNA export (tex) mutants. The tex23-1/rsp5-3 mutant, which is complemented by RSP5, not only shows a strong nuclear accumulation of tRNAs at the restrictive ..