RSC4

Summary

Gene Symbol: RSC4
Description: Rsc4p
Alias: Rsc4p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Damelin M, Simon I, Moy T, Wilson B, Komili S, Tempst P, et al. The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress. Mol Cell. 2002;9:563-73 pubmed
    ..Our results illustrate the response of a chromatin-remodeling factor to signaling cascades and suggest that changes in the activity of chromatin-remodeling factors are reflected in changes in their localization in the genome. ..
  2. Kasten M, Szerlong H, Erdjument Bromage H, Tempst P, Werner M, Cairns B. Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14. EMBO J. 2004;23:1348-59 pubmed
    ..Here, we identify and characterize Rsc4 and show that it bears tandem essential bromodomains...
  3. Soutourina J, Bordas Le Floch V, Gendrel G, Flores A, Ducrot C, Dumay Odelot H, et al. Rsc4 connects the chromatin remodeler RSC to RNA polymerases. Mol Cell Biol. 2006;26:4920-33 pubmed
    RSC is an essential, multisubunit chromatin remodeling complex. We show here that the Rsc4 subunit of RSC interacted via its C terminus with Rpb5, a conserved subunit shared by all three nuclear RNA polymerases (Pol)...
  4. Cairns B, Schlichter A, Erdjument Bromage H, Tempst P, Kornberg R, Winston F. Two functionally distinct forms of the RSC nucleosome-remodeling complex, containing essential AT hook, BAH, and bromodomains. Mol Cell. 1999;4:715-23 pubmed
    ..Therefore, these domains are required for RSC function. Additional genetic analysis provides further evidence that RSC function is related to transcriptional control. ..
  5. Angus Hill M, Schlichter A, Roberts D, Erdjument Bromage H, Tempst P, Cairns B. A Rsc3/Rsc30 zinc cluster dimer reveals novel roles for the chromatin remodeler RSC in gene expression and cell cycle control. Mol Cell. 2001;7:741-51 pubmed
    ..We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC. ..
  6. Campsteijn C, Wijnands Collin A, Logie C. Reverse genetic analysis of the yeast RSC chromatin remodeler reveals a role for RSC3 and SNF5 homolog 1 in ploidy maintenance. PLoS Genet. 2007;3:e92 pubmed
    ..Taken together, our results implicate RSC in regulation of the G1/S-phase transition and establish a hitherto unanticipated role for RSC-mediated chromatin remodeling in ploidy maintenance. ..
  7. Shim E, Ma J, Oum J, Yanez Y, Lee S. The yeast chromatin remodeler RSC complex facilitates end joining repair of DNA double-strand breaks. Mol Cell Biol. 2005;25:3934-44 pubmed
    ..The interaction of Rsc1p with Mre11p appears to be vital for survival from genotoxic stress. These results suggest that chromatin remodeling by RSC is important for NHEJ. ..
  8. VanDemark A, Kasten M, Ferris E, Heroux A, Hill C, Cairns B. Autoregulation of the rsc4 tandem bromodomain by gcn5 acetylation. Mol Cell. 2007;27:817-28 pubmed
    ..The Rsc4 subunit of the yeast remodeler RSC contains an essential tandem bromodomain (TBD) that binds acetylated K14 of ..
  9. Carey M, Li B, Workman J. RSC exploits histone acetylation to abrogate the nucleosomal block to RNA polymerase II elongation. Mol Cell. 2006;24:481-7 pubmed
    ..Thus, RSC can recognize acetylated nucleosomes and facilitate passage of Pol II through them. These data support the view that histone modifications regulate accessibility of the coding region to Pol II. ..

More Information

Publications26

  1. Spain M, Ansari S, Pathak R, Palumbo M, Morse R, Govind C. The RSC complex localizes to coding sequences to regulate Pol II and histone occupancy. Mol Cell. 2014;56:653-66 pubmed publisher
    ..Overall, our findings strongly implicate ORF-associated RSC in governing Pol II function and in maintaining chromatin structure over transcribed regions. ..
  2. Taneda T, Kikuchi A. Genetic analysis of RSC58, which encodes a component of a yeast chromatin remodeling complex, and interacts with the transcription factor Swi6. Mol Genet Genomics. 2004;271:479-89 pubmed
    ..These results suggest that C-terminal region is essential for Rsc58 function. (2) RSC4, which codes for another member of the RSC, was found to be a multicopy suppressor of the phenotype of one of the ..
  3. Saha A, Wittmeyer J, Cairns B. Chromatin remodeling by RSC involves ATP-dependent DNA translocation. Genes Dev. 2002;16:2120-34 pubmed
    ..Nucleosomes bearing nicks are remodeled less efficiently than intact nucleosomes. These results suggest that RSC and Sth1 are DNA translocases that use both DNA translocation and twist to remodel nucleosomes efficiently. ..
  4. Chambers A, Brownlee P, Durley S, Beacham T, Kent N, Downs J. The two different isoforms of the RSC chromatin remodeling complex play distinct roles in DNA damage responses. PLoS ONE. 2012;7:e32016 pubmed publisher
  5. Andress E, Holic R, Edelmann M, Kessler B, Yu V. Dia2 controls transcription by mediating assembly of the RSC complex. PLoS ONE. 2011;6:e21172 pubmed publisher
    ..Dia2, by controlling the RSC chromatin remodeller, fine-tunes transcription by controlling nucleosome positioning during transcriptional activation and repression. ..
  6. Choi J, Grimes D, Rowe K, Howe L. Acetylation of Rsc4p by Gcn5p is essential in the absence of histone H3 acetylation. Mol Cell Biol. 2008;28:6967-72 pubmed publisher
    b>Rsc4p, a subunit of the RSC chromatin-remodeling complex, is acetylated at lysine 25 by Gcn5p, a well-characterized histone acetyltransferase (HAT)...
  7. Charles G, Chen C, Shih S, Collins S, Beltrao P, Zhang X, et al. Site-specific acetylation mark on an essential chromatin-remodeling complex promotes resistance to replication stress. Proc Natl Acad Sci U S A. 2011;108:10620-5 pubmed publisher
    ..RSC is itself acetylated by Gcn5 on lysine 25 (K25) of its Rsc4 subunit, adjacent to two tandem bromodomains...
  8. Zhang Q, Chakravarty S, Ghersi D, Zeng L, Plotnikov A, Sanchez R, et al. Biochemical profiling of histone binding selectivity of the yeast bromodomain family. PLoS ONE. 2010;5:e8903 pubmed publisher
    ..Finally, electrostatic interactions appear to be a primary determining factor that guides productive association between a bromodomain and a lysine-acetylated histone. ..
  9. Szerlong H, Hinata K, Viswanathan R, Erdjument Bromage H, Tempst P, Cairns B. The HSA domain binds nuclear actin-related proteins to regulate chromatin-remodeling ATPases. Nat Struct Mol Biol. 2008;15:469-76 pubmed publisher
    ..Taken together, we define the role of the HSA domain and provide evidence for a regulatory relationship involving the ARP-HSA module and two new functional domains conserved in remodeler ATPases that contain ARPs. ..
  10. Szerlong H, Saha A, Cairns B. The nuclear actin-related proteins Arp7 and Arp9: a dimeric module that cooperates with architectural proteins for chromatin remodeling. EMBO J. 2003;22:3175-87 pubmed
    ..We propose that Arp7/9 dimers function with DNA bending proteins to facilitate proper chromatin architecture and complex- complex interactions. ..
  11. Mirón García M, Garrido Godino A, Martínez Fernández V, Fernández Pévida A, Cuevas Bermúdez A, Martín Expósito M, et al. The yeast prefoldin-like URI-orthologue Bud27 associates with the RSC nucleosome remodeler and modulates transcription. Nucleic Acids Res. 2014;42:9666-76 pubmed publisher
  12. Sengupta S, VanKanegan M, Persinger J, Logie C, Cairns B, Peterson C, et al. The interactions of yeast SWI/SNF and RSC with the nucleosome before and after chromatin remodeling. J Biol Chem. 2001;276:12636-44 pubmed
    ..DNA photoaffinity labeling of RSC showed that the Rsc4 subunit is in close proximity to nucleosomal DNA and not when RSC is bound to free DNA...
  13. Ferreira H, Flaus A, Owen Hughes T. Histone modifications influence the action of Snf2 family remodelling enzymes by different mechanisms. J Mol Biol. 2007;374:563-79 pubmed
    ..These observations illustrate a range of different means by which modifications to histones can influence the action of remodelling enzymes. ..
  14. Fischer C, Saha A, Cairns B. Kinetic model for the ATP-dependent translocation of Saccharomyces cerevisiae RSC along double-stranded DNA. Biochemistry. 2007;46:12416-26 pubmed
    ..These results are further discussed in the context of previously published studies of RSC and other DNA translocases. ..
  15. Lorch Y, Maier Davis B, Kornberg R. Chromatin remodeling by nucleosome disassembly in vitro. Proc Natl Acad Sci U S A. 2006;103:3090-3 pubmed
    ..RSC and related chromatin-remodeling complexes may be responsible for the removal of promoter nucleosomes during transcriptional activation in vivo. ..
  16. Parnell T, Schlichter A, WILSON B, Cairns B. The chromatin remodelers RSC and ISW1 display functional and chromatin-based promoter antagonism. elife. 2015;4:e06073 pubmed publisher
    ..Taken together, we provide the first genetic and genomic evidence for RSC-ISW1a antagonism and reveal different mechanisms at two different promoter architectures. ..
  17. Lia G, Praly E, Ferreira H, Stockdale C, Tse Dinh Y, Dunlap D, et al. Direct observation of DNA distortion by the RSC complex. Mol Cell. 2006;21:417-25 pubmed
    ..These observations support the idea that the ATPase motors of the Snf2 family of proteins act as DNA translocases specialized to generate transient distortions in DNA structure. ..