Rrp5p

Summary

Gene Symbol: Rrp5p
Description: Rrp5p
Alias: Rrp5p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. de Boer P, Vos H, Faber A, Vos J, Raué H. Rrp5p, a trans-acting factor in yeast ribosome biogenesis, is an RNA-binding protein with a pronounced preference for U-rich sequences. RNA. 2006;12:263-71 pubmed
    b>Rrp5p is a trans-acting factor important for biogenesis of both the 40S and 60S subunit of the Saccharomyces cerevisiae ribosome...
  2. Torchet C, Jacq C, Hermann Le Denmat S. Two mutant forms of the S1/TPR-containing protein Rrp5p affect the 18S rRNA synthesis in Saccharomyces cerevisiae. RNA. 1998;4:1636-52 pubmed
    The genetic depletion of yeast Rrp5p results in a synthesis defect of both 18S and 5.8S ribosomal RNAs (Venema J, Tollervey D. 1996. EMBO J 15:5701-5714)...
  3. Young C, Khoshnevis S, Karbstein K. Cofactor-dependent specificity of a DEAD-box protein. Proc Natl Acad Sci U S A. 2013;110:E2668-76 pubmed publisher
    ..Identification of helicase cofactors and their RNA substrates could therefore help define the undescribed roles of the 19 DEAD-box proteins that function in ribosome assembly. ..
  4. Dosil M. Ribosome synthesis-unrelated functions of the preribosomal factor Rrp12 in cell cycle progression and the DNA damage response. Mol Cell Biol. 2011;31:2422-38 pubmed publisher
    ..I propose that the functional duality of Rrp12 may couple the control of ribosome production to the regulation of other cellular processes during cell cycle progression. ..
  5. Venema J, Tollervey D. RRP5 is required for formation of both 18S and 5.8S rRNA in yeast. EMBO J. 1996;15:5701-14 pubmed
    ..Genetic depletion of Rrp5p inhibits the synthesis of 18S rRNA and, unexpectedly, also of the major short form of 5.8S rRNA...
  6. Merl J, Jakob S, Ridinger K, Hierlmeier T, Deutzmann R, Milkereit P, et al. Analysis of ribosome biogenesis factor-modules in yeast cells depleted from pre-ribosomes. Nucleic Acids Res. 2010;38:3068-80 pubmed publisher
    ..The data presented strengthen the idea that assembly of eukaryotic pre-ribosomal particles can result from transient association of distinct building blocks. ..
  7. Dembowski J, Kuo B, Woolford J. Has1 regulates consecutive maturation and processing steps for assembly of 60S ribosomal subunits. Nucleic Acids Res. 2013;41:7889-904 pubmed publisher
    ..Thus, our data support a model in which Has1 helps to establish domain I architecture to prevent pre-rRNA turnover and couples domain I folding with consecutive pre-rRNA processing steps. ..
  8. Choque E, Marcellin M, Burlet Schiltz O, Gadal O, Dez C. The nucleolar protein Nop19p interacts preferentially with Utp25p and Dhr2p and is essential for the production of the 40S ribosomal subunit in Saccharomyces cerevisiae. RNA Biol. 2011;8:1158-72 pubmed publisher
    ..Nop19p appeared essential for the incorporation of Utp25p in preribosomes. In addition, our results suggest that in absence of Nop19p, Dhr2p remains trapped within aberrant preribosomes. ..
  9. Tsvetanova N, Klass D, Salzman J, Brown P. Proteome-wide search reveals unexpected RNA-binding proteins in Saccharomyces cerevisiae. PLoS ONE. 2010;5: pubmed publisher
    ..Our results suggest that many more RBPs still remain to be identified and provide a set of candidates for further investigation. ..

More Information

Publications20

  1. Vos H, Faber A, de Gier M, Vos J, Raué H. Deletion of the three distal S1 motifs of Saccharomyces cerevisiae Rrp5p abolishes pre-rRNA processing at site A(2) without reducing the production of functional 40S subunits. Eukaryot Cell. 2004;3:1504-12 pubmed
    Yeast Rrp5p, one of the few trans-acting proteins required for the biogenesis of both ribosomal subunits, has a remarkable two-domain structure...
  2. Vos H, Bax R, Faber A, Vos J, Raué H. U3 snoRNP and Rrp5p associate independently with Saccharomyces cerevisiae 35S pre-rRNA, but Rrp5p is essential for association of Rok1p. Nucleic Acids Res. 2004;32:5827-33 pubmed
    ..Here, we have studied the mutual dependence between three of these factors, Rrp5p, U3 snoRNP and Rok1p, all essential for the early stages of pre-rRNA processing/assembly, for association with the ..
  3. Eppens N, Faber A, Rondaij M, Jahangir R, van Hemert S, Vos J, et al. Deletions in the S1 domain of Rrp5p cause processing at a novel site in ITS1 of yeast pre-rRNA that depends on Rex4p. Nucleic Acids Res. 2002;30:4222-31 pubmed
    b>Rrp5p is the only protein so far known to be required for the processing of yeast pre-rRNA at both the early sites A0, A1 and A2 leading to 18S rRNA and at site A3, the first step specific for the pathway leading to 5.8S/25S rRNA...
  4. Pandit S, Lynn B, Rymond B. Inhibition of a spliceosome turnover pathway suppresses splicing defects. Proc Natl Acad Sci U S A. 2006;103:13700-5 pubmed
    ..These and related genetic data support the existence of a Spp382p-dependent turnover pathway acting on defective spliceosomes. ..
  5. Hoang T, Peng W, Vanrobays E, Krogan N, Hiley S, Beyer A, et al. Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction. Mol Cell Biol. 2005;25:5523-34 pubmed
    ..The identification of ABT1 in a large-scale analysis of the human nucleolar proteome indicates that its role may also be conserved in mammals. ..
  6. Faber A, Vos J, Vos H, Ghazal G, Elela S, Raué H. The RNA catabolic enzymes Rex4p, Rnt1p, and Dbr1p show genetic interaction with trans-acting factors involved in processing of ITS1 in Saccharomyces cerevisiae pre-rRNA. RNA. 2004;10:1946-56 pubmed
    ..8SL rRNA species is poorly documented. We have previously shown that the trans-acting factor Rrp5p and the RNA exonuclease Rex4p genetically interact to influence the ratio between the two forms of 5...
  7. Shimoji K, Jakovljevic J, Tsuchihashi K, Umeki Y, Wan K, Kawasaki S, et al. Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:4574-88 pubmed publisher
  8. Fath S, Milkereit P, Podtelejnikov A, Bischler N, Schultz P, Bier M, et al. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. J Cell Biol. 2000;149:575-90 pubmed
    ..protein (TBP), Rrn3p, Rrn5p, Rrn7p, and Reb1p along with rRNA processing factors, like Nop1p, Cbf5p, Nhp2p, and Rrp5p. The small nucleolar RNAs (snoRNAs) U3, U14, and MRP were also found to be associated with the complex, which ..
  9. Lebaron S, Segerstolpe A, French S, Dudnakova T, de Lima Alves F, Granneman S, et al. Rrp5 binding at multiple sites coordinates pre-rRNA processing and assembly. Mol Cell. 2013;52:707-19 pubmed publisher
    ..A key role in coordinating preribosomal assembly and processing was confirmed by chromatin spreads. Following depletion of Rrp5, cotranscriptional cleavage was lost and preribosome compaction greatly reduced. ..
  10. Moriggi G, Nieto B, Dosil M. Rrp12 and the Exportin Crm1 participate in late assembly events in the nucleolus during 40S ribosomal subunit biogenesis. PLoS Genet. 2014;10:e1004836 pubmed publisher
  11. Khoshnevis S, Askenasy I, Johnson M, Dattolo M, Young Erdos C, Stroupe M, et al. The DEAD-box Protein Rok1 Orchestrates 40S and 60S Ribosome Assembly by Promoting the Release of Rrp5 from Pre-40S Ribosomes to Allow for 60S Maturation. PLoS Biol. 2016;14:e1002480 pubmed publisher