Gene Symbol: RRD1
Description: peptidylprolyl isomerase RRD1
Alias: YPA1, peptidylprolyl isomerase RRD1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Jouvet N, Poschmann J, Douville J, Bulet L, Ramotar D. Rrd1 isomerizes RNA polymerase II in response to rapamycin. BMC Mol Biol. 2010;11:92 pubmed publisher
    ..Mutants devoid of Rrd1, a protein possessing in vitro peptidyl prolyl cis/trans isomerase activity, display striking resistance to the ..
  2. Jordens J, Janssens V, Longin S, Stevens I, Martens E, Bultynck G, et al. The protein phosphatase 2A phosphatase activator is a novel peptidyl-prolyl cis/trans-isomerase. J Biol Chem. 2006;281:6349-57 pubmed
    ..In this study we demonstrate that PTPA and its yeast homologues Ypa1 and Ypa2 can induce a conformational change in some model substrates...
  3. Ramotar D, Belanger E, Brodeur I, Masson J, Drobetsky E. A yeast homologue of the human phosphotyrosyl phosphatase activator PTPA is implicated in protection against oxidative DNA damage induced by the model carcinogen 4-nitroquinoline 1-oxide. J Biol Chem. 1998;273:21489-96 pubmed
  4. Hombauer H, Weismann D, Mudrak I, Stanzel C, Fellner T, Lackner D, et al. Generation of active protein phosphatase 2A is coupled to holoenzyme assembly. PLoS Biol. 2007;5:e155 pubmed
    ..We propose a novel model of PP2A biogenesis in which a tightly controlled activation cascade protects cells from untargeted activity of the free catalytic PP2A subunit. ..
  5. Leulliot N, Vicentini G, Jordens J, Quevillon Cheruel S, Schiltz M, Barford D, et al. Crystal structure of the PP2A phosphatase activator: implications for its PP2A-specific PPIase activity. Mol Cell. 2006;23:413-24 pubmed
    ..We present here the crystal structures of human PTPA and of the two yeast orthologs (Ypa1 and Ypa2), revealing an all alpha-helical protein fold that is radically different from other PPIases...
  6. Douville J, David J, Lemieux K, Gaudreau L, Ramotar D. The Saccharomyces cerevisiae phosphatase activator RRD1 is required to modulate gene expression in response to rapamycin exposure. Genetics. 2006;172:1369-72 pubmed
    We show that mutants lacking either the phosphatase activator Rrd1 or the phosphatase Pph3 are resistant to rapamycin and that double mutants exhibit a synergistic response...
  7. Fellner T, Lackner D, Hombauer H, Piribauer P, Mudrak I, Zaragoza K, et al. A novel and essential mechanism determining specificity and activity of protein phosphatase 2A (PP2A) in vivo. Genes Dev. 2003;17:2138-50 pubmed
    ..Complementation and RNA-interference experiments showed that PTPA fulfills an essential function conserved from yeast to man. ..
  8. Van Hoof C, Janssens V, De Baere I, Stark M, de Winde J, Winderickx J, et al. The Saccharomyces cerevisiae phosphotyrosyl phosphatase activator proteins are required for a subset of the functions disrupted by protein phosphatase 2A mutations. Exp Cell Res. 2001;264:372-87 pubmed
    In Saccharomyces cerevisiae, PTPA is encoded by two genes, YPA1 and YPA2...
  9. Mitchell D, Sprague G. The phosphotyrosyl phosphatase activator, Ncs1p (Rrd1p), functions with Cla4p to regulate the G(2)/M transition in Saccharomyces cerevisiae. Mol Cell Biol. 2001;21:488-500 pubmed
    ..One group, NCS1/RRD1 (YIL153w), did not confer this defect, however, and we investigated its function further...

More Information


  1. Rempola B, Kaniak A, Migdalski A, Rytka J, Slonimski P, di Rago J. Functional analysis of RRD1 (YIL153w) and RRD2 (YPL152w), which encode two putative activators of the phosphotyrosyl phosphatase activity of PP2A in Saccharomyces cerevisiae. Mol Gen Genet. 2000;262:1081-92 pubmed
    ..Based on the resulting phenotypes, the corresponding genes were named RRD1 and RRD2, respectively...
  2. Douville J, David J, Fortier P, Ramotar D. The yeast phosphotyrosyl phosphatase activator protein, yPtpa1/Rrd1, interacts with Sit4 phosphatase to mediate resistance to 4-nitroquinoline-1-oxide and UVA. Curr Genet. 2004;46:72-81 pubmed
    ..We suggest that the yPtpa1-Sit4 complex may participate in a novel mechanism that mediates repair of oxidative DNA damage caused by 4-nitroquinoline-1-oxide and UVA. ..
  3. Van Hoof C, Martens E, Longin S, Jordens J, Stevens I, Janssens V, et al. Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2. Biochem J. 2005;386:93-102 pubmed
    To elucidate the specific biological role of the yeast homologues of PTPA (phosphatase 2A phosphatase activator), Ypa1 and Ypa2 (where Ypa stands for yeast phosphatase activator), in the regulation of PP2A (protein phosphatase 2A), we ..
  4. Zheng Y, Jiang Y. The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes. Mol Biol Cell. 2005;16:2119-27 pubmed
    ..In yeast Saccharomyces cerevisiae, PTPA is encoded by two related genes, RRD1 and RRD2, whose products are 38 and 37% identical, respectively, to the mammalian PTPA...
  5. Van Hoof C, Janssens V, De Baere I, de Winde J, Winderickx J, Dumortier F, et al. The Saccharomyces cerevisiae homologue YPA1 of the mammalian phosphotyrosyl phosphatase activator of protein phosphatase 2A controls progression through the G1 phase of the yeast cell cycle. J Mol Biol. 2000;302:103-20 pubmed
    The Saccharomyces cerevisiae gene YPA1 encodes a protein homologous to the phosphotyrosyl phosphatase activator, PTPA, of the mammalian protein phosphatase type 2A (PP2A)...
  6. Rempola B, Kaniak A, di Rago J, Rytka J. Anaerobic growth of Saccharomyces cerevisiae alleviates the lethal effect of phosphotyrosyl phosphatase activators depletion. Acta Biochim Pol. 2001;48:1043-9 pubmed
    ..Previously we have shown that the lethality of rrd1,2delta can be suppressed by increasing the osmolarity of the medium...
  7. Scheidt V, Jüdes A, Bär C, Klassen R, Schaffrath R. Loss of wobble uridine modification in tRNA anticodons interferes with TOR pathway signaling. Microb Cell. 2014;1:416-424 pubmed publisher
    ..we examined interaction between mutations in TOR pathway genes (tip41?, sap190?, ppm1?, rrd1?) and U34 modification defects (elp3?, kti12?, urm1?, ncs2?) and found the ..
  8. Marrakchi R, Chouchani C, Poschmann J, Andreev E, Cherif M, Ramotar D. A functional autophagy pathway is required for rapamycin-induced degradation of the Sgs1 helicase in Saccharomyces cerevisiae. Biochem Cell Biol. 2013;91:123-30 pubmed publisher
    ..We propose that defects in the autophagy pathway prevent degradation of key proteins in the rapamycin response pathway and as a consequence cause resistance to the drug. ..
  9. Oler A, Cairns B. PP4 dephosphorylates Maf1 to couple multiple stress conditions to RNA polymerase III repression. EMBO J. 2012;31:1440-52 pubmed publisher
    ..as the main Maf1 phosphatase, and define the involved catalytic (Pph3), scaffold (Psy2) and regulatory subunits (Rrd1, Tip41), as well as uninvolved subunits (Psy4, Rrd2)...
  10. Marrakchi R, Chouchani C, Cherif M, Boudabbous A, Ramotar D. The isomerase Rrd1 mediates rapid loss of the Sgs1 helicase in response to rapamycin. Biochem Cell Biol. 2011;89:332-40 pubmed publisher
    ..Deletion of the RRD1 gene encoding a peptidyl prolyl isomerase resulted in mutants that are resistant to rapamycin...
  11. Van Hoof C, Cayla X, Bosch M, Merlevede W, Goris J. The phosphotyrosyl phosphatase activator of protein phosphatase 2A. A novel purification method, immunological and enzymic characterization. Eur J Biochem. 1994;226:899-907 pubmed
    ..Mg, a necessary cofactor in the activation process. Interaction of PTPA with PP2A in a 1:1 ratio induces a low (kcat = 3 min-1) ATPase activity that is inhibited by okadaic acid, ADP and non-hydrolysable ATP analogues. ..
  12. Poschmann J, Drouin S, Jacques P, El Fadili K, Newmarch M, Robert F, et al. The peptidyl prolyl isomerase Rrd1 regulates the elongation of RNA polymerase II during transcriptional stresses. PLoS ONE. 2011;6:e23159 pubmed publisher
    ..In yeast, rapamycin mediates a profound transcriptional response for which the RRD1 gene is required...
  13. Castermans D, Somers I, Kriel J, Louwet W, Wera S, Versele M, et al. Glucose-induced posttranslational activation of protein phosphatases PP2A and PP1 in yeast. Cell Res. 2012;22:1058-77 pubmed publisher
    ..Glucose activation of PP2A is controlled by regulatory subunits Rts1, Cdc55, Rrd1 and Rrd2...
  14. Jablonowski D, Täubert J, Bär C, Stark M, Schaffrath R. Distinct subsets of Sit4 holophosphatases are required for inhibition of Saccharomyces cerevisiae growth by rapamycin and zymocin. Eukaryot Cell. 2009;8:1637-47 pubmed publisher
    ..Similarly, the rapamycin resistance of an rrd1Delta mutant lacking the Sit4 interactor Rrd1 specifically requires the Sit4/Sap190 complex...
  15. Zhang W, Durocher D. De novo telomere formation is suppressed by the Mec1-dependent inhibition of Cdc13 accumulation at DNA breaks. Genes Dev. 2010;24:502-15 pubmed publisher
    ..Pph3 is itself modulated by Rrd1, an activator of PP2A family phosphatases...
  16. Palmer L, Baptiste B, Fester J, Perkins J, Keil R. RRD1, a component of the TORC1 signalling pathway, affects anaesthetic response in Saccharomyces cerevisiae. Yeast. 2009;26:655-61 pubmed publisher
    ..cerevisiae resistant to the volatile anaesthetic isoflurane. One of these genes, RRD1, encodes a subunit of the Tap42p-Sit4p-Rrd1p phosphatase complex that functions in the target of rapamycin complex ..
  17. Van Hoof C, Janssens V, Dinishiotu A, Merlevede W, Goris J. Functional analysis of conserved domains in the phosphotyrosyl phosphatase activator. Molecular cloning of the homologues from Drosophila melanogaster and Saccharomyces cerevisiae. Biochemistry. 1998;37:12899-908 pubmed
    ..Moreover, we observed that PTPA truncated at glycine266 behaves as a dominant negative mutant since it is inhibitory to the wild-type PTPA. ..