Gene Symbol: RPS2
Description: ribosomal 40S subunit protein S2
Alias: RPS4, SUP138, SUP38, SUP44, ribosomal 40S subunit protein S2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Chiu W, Wagner S, Herrmannová A, Burela L, Zhang F, Saini A, et al. The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Mol Cell Biol. 2010;30:4415-34 pubmed publisher
    ..These results, and our demonstration that the extreme CTD of a/Tif32 binds to Rps2 and Rps3, lead us to propose that the a/Tif32 CTD directly stabilizes 43S subunit-mRNA interaction and that the b/..
  2. Merwin J, Bogar L, Poggi S, Fitch R, Johnson A, Lycan D. Genetic analysis of the ribosome biogenesis factor Ltv1 of Saccharomyces cerevisiae. Genetics. 2014;198:1071-85 pubmed publisher
    ..We suggest that titration of RpS3 by excess nuclear Ltv1 interferes with 40S function or with a nonribosomal function of RpS3. ..
  3. Young B, Weiss D, Zurita Lopez C, Webb K, Clarke S, McBride A. Identification of methylated proteins in the yeast small ribosomal subunit: a role for SPOUT methyltransferases in protein arginine methylation. Biochemistry. 2012;51:5091-104 pubmed publisher
    We have characterized the posttranslational methylation of Rps2, Rps3, and Rps27a, three small ribosomal subunit proteins in the yeast Saccharomyces cerevisiae, using mass spectrometry and amino acid analysis...
  4. Higo K, Otaka E. Isolation and characterization of fourteen ribosomal proteins from small subunits of yeast. Biochemistry. 1979;18:4191-6 pubmed
    ..The isolated proteins are YP 6, YP 7, YP 9, YP 12, YP 14', YP 14'', YP 28, YP 38, YP 45, YP 50, YP 52, YP 58, YP 63, and YP 70. The molecular weight and amino acid compositions of these proteins are presented. ..
  5. Yagoub D, Hart Smith G, Moecking J, Erce M, Wilkins M. Yeast proteins Gar1p, Nop1p, Npl3p, Nsr1p, and Rps2p are natively methylated and are substrates of the arginine methyltransferase Hmt1p. Proteomics. 2015;15:3209-18 pubmed publisher
    ..This brings the total of Hmt1 substrate proteins for which native methylation sites have been identified to five. ..
  6. Baierlein C, Hackmann A, Gross T, Henker L, Hinz F, Krebber H. Monosome formation during translation initiation requires the serine/arginine-rich protein Npl3. Mol Cell Biol. 2013;33:4811-23 pubmed publisher
    ..These findings indicate an essential novel function for Npl3 in the cytoplasm, which supports monosome formation for translation initiation. ..
  7. Fujii K, Kitabatake M, Sakata T, Ohno M. 40S subunit dissociation and proteasome-dependent RNA degradation in nonfunctional 25S rRNA decay. EMBO J. 2012;31:2579-89 pubmed publisher
  8. Ladror D, Frey B, Scalf M, Levenstein M, Artymiuk J, Smith L. Methylation of yeast ribosomal protein S2 is elevated during stationary phase growth conditions. Biochem Biophys Res Commun. 2014;445:535-41 pubmed publisher
    ..ribosomal protein S1B (RPS1B), and a 10-fold increase in dimethylation for r(DiMe)GGFGGR of ribosomal protein S2 (RPS2)...
  9. Röther S, Strasser K. The RNA polymerase II CTD kinase Ctk1 functions in translation elongation. Genes Dev. 2007;21:1409-21 pubmed
    ..Ctk1 associates with translating ribosomes in vivo and is needed for efficient translation. Ctk1 phosphorylates Rps2, a protein of the small ribosomal subunit, on Ser 238...

More Information


  1. Lipson R, Webb K, Clarke S. Rmt1 catalyzes zinc-finger independent arginine methylation of ribosomal protein Rps2 in Saccharomyces cerevisiae. Biochem Biophys Res Commun. 2010;391:1658-62 pubmed publisher
    b>Rps2/rpS2 is a well conserved protein of the eukaryotic ribosomal small subunit...
  2. Xiang R, Lee J. Identification of proteins crosslinked to RNA in 40S ribosomal subunits of Saccharomyces cerevisiae. Biochimie. 1989;71:1201-4 pubmed
    ..A shorter period of irradiation resulted in crosslinking of S2 and S17/22/27 only. Several of these proteins were previously demonstrated to be present in ribosomal core particles or early assembled proteins. ..
  3. Schaper S, Fromont Racine M, Linder P, De la Cruz J, Namane A, Yaniv M. A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly. Curr Biol. 2001;11:1885-90 pubmed
    ..This action may resemble the mechanism by which CAF1 assembles histones H3/H4 onto newly replicated DNA. ..
  4. Yeh Y, Traut R, Lee J. Protein topography of the 40 S ribosomal subunit from Saccharomyces cerevisiae as shown by chemical cross-linking. J Biol Chem. 1986;261:14148-53 pubmed
    ..Many proteins were detected in several cross-linked dimers. These proteins with multiple cross-links form foci for the construction of a schematic model of the spatial arrangement of proteins within the 40 S subunit. ..
  5. Kaplan Y, Kupiec M. A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition. Curr Genet. 2007;51:123-40 pubmed
    ..Finally, we discuss possible mechanisms of suppression by the cDNAs that imply cell cycle regulation by apparently unrelated processes, such as splicing, translation initiation and glycolysis. ..
  6. Peng W, Krogan N, Richards D, Greenblatt J, Hughes T. ESF1 is required for 18S rRNA synthesis in Saccharomyces cerevisiae. Nucleic Acids Res. 2004;32:1993-9 pubmed
    ..These data indicate that Esf1p plays a direct role in early pre-rRNA processing. ..
  7. Koch B, Mitterer V, Niederhauser J, Stanborough T, Murat G, Rechberger G, et al. Yar1 protects the ribosomal protein Rps3 from aggregation. J Biol Chem. 2012;287:21806-15 pubmed publisher
    ..Our data suggest that Yar1 is a specific chaperone for Rps3, which serves to keep Rps3 soluble until its incorporation into the pre-ribosome. ..
  8. ElAntak L, Wagner S, Herrmannová A, Karásková M, Rutkai E, Lukavsky P, et al. The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection. J Mol Biol. 2010;396:1097-116 pubmed publisher
    ..binding site for human eIF3j by identifying specific interactions of eIF3j/HCR1 with small ribosomal proteins RPS2 and RPS23 located in the vicinity of the mRNA entry channel...
  9. Hoang T, Peng W, Vanrobays E, Krogan N, Hiley S, Beyer A, et al. Esf2p, a U3-associated factor required for small-subunit processome assembly and compaction. Mol Cell Biol. 2005;25:5523-34 pubmed
    ..The identification of ABT1 in a large-scale analysis of the human nucleolar proteome indicates that its role may also be conserved in mammals. ..
  10. Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher
    ..Thus, cells must have a way of ensuring a proper balance between actin assembly pathways. ..
  11. Eustice D, Wakem L, Wilhelm J, Sherman F. Altered 40 S ribosomal subunits in omnipotent suppressors of yeast. J Mol Biol. 1986;188:207-14 pubmed
    The five suppressors SUP35, SUP43, SUP44, SUP45 and SUP46, each mapping at a different chromosomal locus in the yeast Saccharomyces cerevisiae, suppress a wide range of mutations, including representatives of all three types of nonsense ..
  12. Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillère Q, Braunger K, Heuer A, et al. The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex. Science. 2016;354:1431-1433 pubmed
    ..We observe that the mRNA 3' overhang is threaded directly from the small ribosomal subunit to the helicase channel of Ski2, primed for ongoing exosome-mediated 3'-5' degradation. ..
  13. Van Dyke M, Nelson L, Weilbaecher R, Mehta D. Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, interacts with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae. J Biol Chem. 2004;279:24323-33 pubmed
    ..We discuss the implications of our findings in relation to prior genetic, genomic, and proteomic studies that have identified STM1 and/or Stm1p as well as the possible biological role of Stm1p. ..
  14. Schmitt K, Smolinski N, Neumann P, Schmaul S, Hofer Pretz V, Braus G, et al. Asc1p/RACK1 Connects Ribosomes to Eukaryotic Phosphosignaling. Mol Cell Biol. 2017;37: pubmed publisher
    ..Our data expose Asc1p/RACK1 as a key factor in phosphosignaling and manifest it as a control point at the head of the ribosomal 40S subunit itself regulated through posttranslational modification. ..