Gene Symbol: RPL5
Description: ribosomal 60S subunit protein L5
Alias: LPI14, RPL1, ribosomal 60S subunit protein L5
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Saveanu C, Bienvenu D, Namane A, Gleizes P, Gas N, Jacquier A, et al. Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps. EMBO J. 2001;20:6475-84 pubmed
    ..These results suggest that transient, possibly GTP-dependent association of Nog2p with the pre-ribosomes might trigger late rRNA maturation steps in ribosomal large subunit biogenesis. ..
  2. Honma Y, Kitamura A, Shioda R, Maruyama H, Ozaki K, Oda Y, et al. TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients. EMBO J. 2006;25:3832-42 pubmed
    ..Thus, the Nog1 complex is a critical regulator of ribosome biogenesis mediated by TOR. This is the first description of a physiological regulation of nucleolus-to-nucleoplasm translocation of pre-ribosome complexes. ..
  3. Zhang J, Harnpicharnchai P, Jakovljevic J, Tang L, Guo Y, Oeffinger M, et al. Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes. Genes Dev. 2007;21:2580-92 pubmed
    ..in preribosomes that contains two yeast ribosome assembly factors, Rpf2 and Rrs1, two ribosomal proteins, rpL5 and rpL11, and 5S rRNA...
  4. Deshmukh M, Tsay Y, Paulovich A, Woolford J. Yeast ribosomal protein L1 is required for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits. Mol Cell Biol. 1993;13:2835-45 pubmed
    ..interaction between L1 and 5S rRNA and to assess the role of L1 in ribosome assembly and function, we cloned the RPL1 gene encoding L1. We have shown that RPL1 is an essential single-copy gene...
  5. Lebreton A, Saveanu C, Decourty L, Jacquier A, Fromont Racine M. Nsa2 is an unstable, conserved factor required for the maturation of 27 SB pre-rRNAs. J Biol Chem. 2006;281:27099-108 pubmed
    ..Indeed, when ribosome biogenesis was blocked upstream of Nsa2, this short half-lived protein was largely depleted, suggesting that its cellular levels are tightly regulated. ..
  6. Shimoji K, Jakovljevic J, Tsuchihashi K, Umeki Y, Wan K, Kawasaki S, et al. Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:4574-88 pubmed publisher
  7. Van Dyke M, Nelson L, Weilbaecher R, Mehta D. Stm1p, a G4 quadruplex and purine motif triplex nucleic acid-binding protein, interacts with ribosomes and subtelomeric Y' DNA in Saccharomyces cerevisiae. J Biol Chem. 2004;279:24323-33 pubmed
    ..We discuss the implications of our findings in relation to prior genetic, genomic, and proteomic studies that have identified STM1 and/or Stm1p as well as the possible biological role of Stm1p. ..
  8. Nariai M, Tanaka T, Okada T, Shirai C, Horigome C, Mizuta K. Synergistic defect in 60S ribosomal subunit assembly caused by a mutation of Rrs1p, a ribosomal protein L11-binding protein, and 3'-extension of 5S rRNA in Saccharomyces cerevisiae. Nucleic Acids Res. 2005;33:4553-62 pubmed
    ..The rrs1-5 mutation weakens the interaction between Rrs1p with both L5 and L11. These data suggest that the assembly of L5-5S rRNA on 60S ribosomal subunits coordinates with assembly of L11 via Rrs1p. ..
  9. Yao Y, Demoinet E, Saveanu C, Lenormand P, Jacquier A, Fromont Racine M. Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export. RNA. 2010;16:1007-17 pubmed publisher
    ..These data highlight the involvement of many factors acting together to export pre-60S particles. ..

More Information


  1. Santos M, Goldfeder M, Zanchin N, Oliveira C. The essential nucleolar yeast protein Nop8p controls the exosome function during 60S ribosomal subunit maturation. PLoS ONE. 2011;6:e21686 pubmed publisher
    ..These results strongly indicate that Nop8p may control the exosome function during pre-rRNA processing. ..
  2. Talkish J, Zhang J, Jakovljevic J, Horsey E, Woolford J. Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:8646-61 pubmed
    ..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes. ..
  3. Kressler D, Bange G, Ogawa Y, Stjepanovic G, Bradatsch B, Pratte D, et al. Synchronizing nuclear import of ribosomal proteins with ribosome assembly. Science. 2012;338:666-71 pubmed publisher
    ..a transport adaptor, symportin 1 (Syo1), that facilitates synchronized coimport of the two 5S-rRNA binding proteins Rpl5 and Rpl11...
  4. Galvão F, Rossi D, Silveira W, Valentini S, Zanelli C. The deoxyhypusine synthase mutant dys1-1 reveals the association of eIF5A and Asc1 with cell wall integrity. PLoS ONE. 2013;8:e60140 pubmed publisher
    ..These data suggest a correlated role for eIF5A and Asc1 in coordinating the translational control of a subset of mRNAs associated with cell integrity. ..
  5. Yeh L, Deshmukh M, Woolford J, Lee J. Involvement of lysine 270 and lysine 271 of yeast 5S rRNA binding protein in RNA binding and ribosome assembly. Biochim Biophys Acta. 1996;1308:133-41 pubmed
    ..The in vitro RNA binding ability of the respective protein was reduced by 60% and 20%. Taken together, the present data identified K270 and K271 as important amino-acid residues in the function of the yeast ribosomal protein L1. ..
  6. Lee R, Brunette S, Puente L, Megeney L. Metacaspase Yca1 is required for clearance of insoluble protein aggregates. Proc Natl Acad Sci U S A. 2010;107:13348-53 pubmed publisher
    ..Together, our results show that Yca1 contributes to the fitness and adaptability of growing yeast through an aggregate remodeling activity. ..
  7. Gómez Herreros F, Rodríguez Galán O, Morillo Huesca M, Maya D, Arista Romero M, de la Cruz J, et al. Balanced production of ribosome components is required for proper G1/S transition in Saccharomyces cerevisiae. J Biol Chem. 2013;288:31689-700 pubmed publisher
    ..This regulatory pathway resembles the p53-dependent nucleolar-stress checkpoint response described in human cells, which indicates that this is a general control strategy extended throughout eukaryotes. ..