RPG1

Summary

Gene Symbol: RPG1
Description: translation initiation factor eIF3 core subunit a
Alias: TIF32, translation initiation factor eIF3 core subunit a
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. VALASEK L, Nielsen K, Hinnebusch A. Direct eIF2-eIF3 contact in the multifactor complex is important for translation initiation in vivo. EMBO J. 2002;21:5886-98 pubmed
    ..Here we incorporated affinity tags into the three largest eIF3 subunits (eIF3a/TIF32, eIF3b/PRT1 and eIF3c/NIP1) and deleted predicted binding domains in each tagged protein...
  2. VALASEK L, Hasek J, Trachsel H, Imre E, Ruis H. The Saccharomyces cerevisiae HCR1 gene encoding a homologue of the p35 subunit of human translation initiation factor 3 (eIF3) is a high copy suppressor of a temperature-sensitive mutation in the Rpg1p subunit of yeast eIF3. J Biol Chem. 1999;274:27567-72 pubmed
    ..Recently, we described the identification of RPG1 (TIF32), the p110 subunit of the eIF3 core complex in yeast...
  3. Khoshnevis S, Hauer F, Milon P, Stark H, Ficner R. Novel insights into the architecture and protein interaction network of yeast eIF3. RNA. 2012;18:2306-19 pubmed publisher
  4. Kouba T, Dányi I, Gunišová S, Munzarová V, Vlčková V, Cuchalová L, et al. Small ribosomal protein RPS0 stimulates translation initiation by mediating 40S-binding of eIF3 via its direct contact with the eIF3a/TIF32 subunit. PLoS ONE. 2012;7:e40464 pubmed publisher
    ..Among them is the interaction between the N-terminal domain (NTD) of the a/TIF32 subunit of eIF3 and the small ribosomal protein RPS0A, residing near the mRNA exit channel...
  5. Zhou M, Sandercock A, Fraser C, Ridlova G, Stephens E, Schenauer M, et al. Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proc Natl Acad Sci U S A. 2008;105:18139-44 pubmed publisher
  6. Harel Sharvit L, Eldad N, Haimovich G, Barkai O, Duek L, Choder M. RNA polymerase II subunits link transcription and mRNA decay to translation. Cell. 2010;143:552-63 pubmed publisher
    ..We propose that Rpb4/7, through its interactions at each step in the mRNA lifecycle, represents a class of factors, "mRNA coordinators," which integrate the various stages of gene expression into a system. ..
  7. Phan L, Schoenfeld L, VALASEK L, Nielsen K, Hinnebusch A. A subcomplex of three eIF3 subunits binds eIF1 and eIF5 and stimulates ribosome binding of mRNA and tRNA(i)Met. EMBO J. 2001;20:2954-65 pubmed
    Yeast translation initiation factor 3 contains five core subunits (known as TIF32, PRT1, NIP1, TIF34 and TIF35) and a less tightly associated component known as HCR1...
  8. Asano K, Clayton J, Shalev A, Hinnebusch A. A multifactor complex of eukaryotic initiation factors, eIF1, eIF2, eIF3, eIF5, and initiator tRNA(Met) is an important translation initiation intermediate in vivo. Genes Dev. 2000;14:2534-46 pubmed
    ..We propose that the multifactor complex is an important intermediate in translation initiation in vivo. ..
  9. Vornlocher H, Hanachi P, Ribeiro S, Hershey J. A 110-kilodalton subunit of translation initiation factor eIF3 and an associated 135-kilodalton protein are encoded by the Saccharomyces cerevisiae TIF32 and TIF31 genes. J Biol Chem. 1999;274:16802-12 pubmed
    ..Using partial peptide sequences generated from proteins in purified eIF3, we cloned the TIF31 and TIF32 genes encoding 135- (p135) and 110-kDa (p110) proteins...

More Information

Publications36

  1. Cuchalová L, Kouba T, Herrmannová A, Dányi I, Chiu W, Valásek L. The RNA recognition motif of eukaryotic translation initiation factor 3g (eIF3g) is required for resumption of scanning of posttermination ribosomes for reinitiation on GCN4 and together with eIF3i stimulates linear scanning. Mol Cell Biol. 2010;30:4671-86 pubmed publisher
    ..Together these results implicate g/Tif35 and i/Tif34 in stimulation of linear scanning and, specifically in the case of g/Tif35, also in proper regulation of the GCN4 reinitiation mechanism. ..
  2. Kouba T, Rutkai E, Karásková M, Valášek L. The eIF3c/NIP1 PCI domain interacts with RNA and RACK1/ASC1 and promotes assembly of translation preinitiation complexes. Nucleic Acids Res. 2012;40:2683-99 pubmed publisher
  3. VALASEK L, Trachsel H, Hasek J, Ruis H. Rpg1, the Saccharomyces cerevisiae homologue of the largest subunit of mammalian translation initiation factor 3, is required for translational activity. J Biol Chem. 1998;273:21253-60 pubmed
    ..The product of the Saccharomyces cerevisiae RPG1 gene has been described as encoding a protein required for passage through the G1 phase of the cell cycle and ..
  4. Chiu W, Wagner S, Herrmannová A, Burela L, Zhang F, Saini A, et al. The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons. Mol Cell Biol. 2010;30:4415-34 pubmed publisher
    The C-terminal domain (CTD) of the a/Tif32 subunit of budding yeast eukaryotic translation initiation factor 3 (eIF3) interacts with eIF3 subunits j/Hcr1 and b/Prt1 and can bind helices 16 to 18 of 18S rRNA, suggesting proximity to the ..
  5. Phan L, Zhang X, Asano K, Anderson J, Vornlocher H, Greenberg J, et al. Identification of a translation initiation factor 3 (eIF3) core complex, conserved in yeast and mammals, that interacts with eIF5. Mol Cell Biol. 1998;18:4935-46 pubmed
    ..Thus, eIF5 and Sui1p may be recruited to the 40S ribosomes through physical interactions with the Nip1p subunit of eIF3. ..
  6. Szamecz B, Rutkai E, Cuchalová L, Munzarová V, Herrmannová A, Nielsen K, et al. eIF3a cooperates with sequences 5' of uORF1 to promote resumption of scanning by post-termination ribosomes for reinitiation on GCN4 mRNA. Genes Dev. 2008;22:2414-25 pubmed publisher
  7. Beznosková P, Cuchalová L, Wagner S, Shoemaker C, Gunisova S, von der Haar T, et al. Translation initiation factors eIF3 and HCR1 control translation termination and stop codon read-through in yeast cells. PLoS Genet. 2013;9:e1003962 pubmed publisher
    ..Together our work characterizes novel roles of eIF3 and HCR1 in stop codon recognition, defining a communication bridge between the initiation and termination/recycling phases of translation. ..
  8. ElAntak L, Wagner S, Herrmannová A, Karásková M, Rutkai E, Lukavsky P, et al. The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection. J Mol Biol. 2010;396:1097-116 pubmed publisher
    ..Taken together, we propose that eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RRM and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition. ..
  9. VALASEK L, Mathew A, Shin B, Nielsen K, Szamecz B, Hinnebusch A. The yeast eIF3 subunits TIF32/a, NIP1/c, and eIF5 make critical connections with the 40S ribosome in vivo. Genes Dev. 2003;17:786-99 pubmed
    ..We found that the N- and C-terminal domains of NIP1/eIF3c, the N- and C-terminal domains of TIF32/eIF3a, and eIF5 have critical functions in 40S binding, with eIF5 and the TIF32-CTD performing redundant functions...
  10. VALASEK L, Phan L, Schoenfeld L, Valášková V, Hinnebusch A. Related eIF3 subunits TIF32 and HCR1 interact with an RNA recognition motif in PRT1 required for eIF3 integrity and ribosome binding. EMBO J. 2001;20:891-904 pubmed
    ..motif (RRM) of eIF3 subunit PRT1 interacted simultaneously with HCR1 and with an internal domain of eIF3 subunit TIF32 that has sequence and functional similarity to HCR1...
  11. Asano K, Phan L, Anderson J, Hinnebusch A. Complex formation by all five homologues of mammalian translation initiation factor 3 subunits from yeast Saccharomyces cerevisiae. J Biol Chem. 1998;273:18573-85 pubmed
    ..Although TIF32, NIP1, and TIF35 are homologous to subunits of human eIF3, they were not known to be components of the yeast ..
  12. Nielsen K, VALASEK L, Sykes C, Jivotovskaya A, Hinnebusch A. Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast. Mol Cell Biol. 2006;26:2984-98 pubmed
    ..initiation factor 3 (eIF3) subunit b/PRT1 (prt1-rnp1) impairs its direct interactions in vitro with both eIF3a/TIF32 and eIF3j/HCR1...
  13. Khoshnevis S, Gunisova S, Vlčková V, Kouba T, Neumann P, Beznosková P, et al. Structural integrity of the PCI domain of eIF3a/TIF32 is required for mRNA recruitment to the 43S pre-initiation complexes. Nucleic Acids Res. 2014;42:4123-39 pubmed publisher
    ..To assess the role of the second PCI domain of eIF3 present in eIF3a/TIF32, we performed its mutational analysis and identified a 10-Ala-substitution (Box37) that severely reduces amounts ..
  14. Hinnebusch A, Asano K, Olsen D, Phan L, Nielsen K, VALASEK L. Study of translational control of eukaryotic gene expression using yeast. Ann N Y Acad Sci. 2004;1038:60-74 pubmed
    ..Thus, apart from its critical role in the starvation response, GCN4 regulation is a valuable tool for dissecting the contributions of multiple translation factors in the eukaryotic initiation pathway. ..
  15. Duan R, Rhie B, Ryu H, Ahn S. The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process. Biochem Biophys Res Commun. 2013;: pubmed publisher
  16. Hasek J, Kovarik P, VALASEK L, Malinska K, Schneider J, Kohlwein S, et al. Rpg1p, the subunit of the Saccharomyces cerevisiae eIF3 core complex, is a microtubule-interacting protein. Cell Motil Cytoskeleton. 2000;45:235-46 pubmed
    The essential gene RPG1/TIF32 of Saccharomyces cerevisiae encodes the 110-kDa subunit of the translation initiation factor 3 (eIF3) core complex...
  17. Gomar Alba M, Jimenez Marti E, del Olmo M. The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress. BMC Mol Biol. 2012;13:19 pubmed publisher
  18. Hasek J, Perinka L, VALASEK L. Specification of the monoclonal antibody PK1 reactivity in Chinese hamster ovary cells. Folia Biol (Praha). 2005;51:50-1 pubmed
  19. Castelli L, Lui J, Campbell S, Rowe W, Zeef L, Holmes L, et al. Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated. Mol Biol Cell. 2011;22:3379-93 pubmed publisher
  20. Erzberger J, Stengel F, Pellarin R, Zhang S, Schaefer T, Aylett C, et al. Molecular architecture of the 40Sâ‹…eIF1â‹…eIF3 translation initiation complex. Cell. 2014;158:1123-1135 pubmed publisher
    ..The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA. ..
  21. Gao L, Bretscher A. Analysis of unregulated formin activity reveals how yeast can balance F-actin assembly between different microfilament-based organizations. Mol Biol Cell. 2008;19:1474-84 pubmed publisher
    ..Thus, cells must have a way of ensuring a proper balance between actin assembly pathways. ..
  22. Palecek J, Hasek J, Ruis H. Rpg1p/Tif32p, a subunit of translation initiation factor 3, interacts with actin-associated protein Sla2p. Biochem Biophys Res Commun. 2001;282:1244-50 pubmed
    ..Considering the essential role of Rpg1p as the large subunit of the eIF3 core complex and the association of Sla2p with the actin cytoskeleton, a putative role of the Rpg1p-Sla2p interaction in localized translation is discussed. ..
  23. Beaufour M, Godin F, Vallee B, Cadene M, Bénédetti H. Interaction proteomics suggests a new role for the Tfs1 protein in yeast. J Proteome Res. 2012;11:3211-8 pubmed publisher
    ..Integration of these results with known functional partners of Tfs1p shows that two subnetworks meet through the Tfs1p node, suggesting that it may act as a bridge between cell signaling and intermediate metabolism in yeast. ..
  24. Shalev A, VALASEK L, Pise Masison C, Radonovich M, Phan L, Clayton J, et al. Saccharomyces cerevisiae protein Pci8p and human protein eIF3e/Int-6 interact with the eIF3 core complex by binding to cognate eIF3b subunits. J Biol Chem. 2001;276:34948-57 pubmed
    ..We discuss possible dual functions of Pci8p and Int-6 in transcriptional and translational control. ..
  25. Farley A, Powell D, Weaver C, Jennings J, Link A. Assessing the components of the eIF3 complex and their phosphorylation status. J Proteome Res. 2011;10:1481-94 pubmed publisher
    ..The yeast eIF3 complex contains five core components: Rpg1, Nip1, Prt1, Tif34, and Tif35...
  26. Duan R, Rhie B, Ryu H, Ahn S. The RNA polymerase II Rpb4/7 subcomplex regulates cellular lifespan through an mRNA decay process. Biochem Biophys Res Commun. 2013;441:266-70 pubmed
  27. Grousl T, Ivanov P, Frýdlová I, Vasicová P, Janda F, Vojtova J, et al. Robust heat shock induces eIF2alpha-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae. J Cell Sci. 2009;122:2078-88 pubmed publisher
    ..We conclude that under specific stress conditions, such as robust heat shock, yeast SGs do contain eIF3 and 40S ribosomes and utilize alternative routes for their assembly. ..