RPF2

Summary

Gene Symbol: RPF2
Description: rRNA-binding ribosome biosynthesis protein RPF2
Alias: rRNA-binding ribosome biosynthesis protein RPF2
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Miles T, Jakovljevic J, Horsey E, Harnpicharnchai P, Tang L, Woolford J. Ytm1, Nop7, and Erb1 form a complex necessary for maturation of yeast 66S preribosomes. Mol Cell Biol. 2005;25:10419-32 pubmed
    ..Mutations in the WD40 motifs of Ytm1 disrupt binding to Erb1, destabilize the heterotrimer, and delay pre-rRNA processing and nuclear export of preribosomes. Nevertheless, 66S preribosomes lacking Ytm1 remain otherwise intact. ..
  2. Shimoji K, Jakovljevic J, Tsuchihashi K, Umeki Y, Wan K, Kawasaki S, et al. Ebp2 and Brx1 function cooperatively in 60S ribosomal subunit assembly in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:4574-88 pubmed publisher
  3. Wehner K, Baserga S. The sigma(70)-like motif: a eukaryotic RNA binding domain unique to a superfamily of proteins required for ribosome biogenesis. Mol Cell. 2002;9:329-39 pubmed
    ..The Imp4 superfamily is composed of five individual families (Imp4, Rpf1, Rpf2, Brx1, and Ssf) that all possess the sigma(70)-like motif, a eukaryotic RNA binding domain with prokaryotic origins...
  4. Zhang J, Harnpicharnchai P, Jakovljevic J, Tang L, Guo Y, Oeffinger M, et al. Assembly factors Rpf2 and Rrs1 recruit 5S rRNA and ribosomal proteins rpL5 and rpL11 into nascent ribosomes. Genes Dev. 2007;21:2580-92 pubmed
    ..We identified a ribonucleoprotein neighborhood in preribosomes that contains two yeast ribosome assembly factors, Rpf2 and Rrs1, two ribosomal proteins, rpL5 and rpL11, and 5S rRNA...
  5. Morita D, Miyoshi K, Matsui Y, Toh e A, Shinkawa H, Miyakawa T, et al. Rpf2p, an evolutionarily conserved protein, interacts with ribosomal protein L11 and is essential for the processing of 27 SB Pre-rRNA to 25 S rRNA and the 60 S ribosomal subunit assembly in Saccharomyces cerevisiae. J Biol Chem. 2002;277:28780-6 pubmed
    ..In two-hybrid screening, using RRS1 as bait, we have cloned YKR081c/RPF2. Rpf2p is essential for growth and is mainly localized in the nucleolus...
  6. Talkish J, Zhang J, Jakovljevic J, Horsey E, Woolford J. Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:8646-61 pubmed
    ..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes.
  7. Bogengruber E, Briza P, Doppler E, Wimmer H, Koller L, Fasiolo F, et al. Functional analysis in yeast of the Brix protein superfamily involved in the biogenesis of ribosomes. FEMS Yeast Res. 2003;3:35-43 pubmed
    ..Moreover, all four proteins closely interact functionally, because all four mutants are suppressed by the same multicopy suppressor gene. ..
  8. Matsuo Y, Granneman S, Thoms M, Manikas R, Tollervey D, Hurt E. Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export. Nature. 2014;505:112-116 pubmed publisher
    ..We conclude that Nug2 is a regulatory GTPase that monitors pre-60S maturation, with release from its placeholder site linked to recruitment of the nuclear export machinery. ..
  9. Madru C, Lebaron S, Blaud M, Delbos L, Pipoli J, Pasmant E, et al. Chaperoning 5S RNA assembly. Genes Dev. 2015;29:1432-46 pubmed publisher
    ..localization of the 5S RNP and its assembly into preribosomes are performed by a specialized complex composed of Rpf2 and Rrs1 in yeast or Bxdc1 and hRrs1 in humans...

More Information

Publications14

  1. Fuentes J, Datta K, Sullivan S, Walker A, Maddock J. In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics. 2007;278:105-23 pubmed
    ..Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function. ..
  2. Tang L, Sahasranaman A, Jakovljevic J, Schleifman E, Woolford J. Interactions among Ytm1, Erb1, and Nop7 required for assembly of the Nop7-subcomplex in yeast preribosomes. Mol Biol Cell. 2008;19:2844-56 pubmed publisher
    ..Our data suggest a model for the architecture of the Nop7-subcomplex and provide potential functions of domains of each protein. ..
  3. Dembowski J, Kuo B, Woolford J. Has1 regulates consecutive maturation and processing steps for assembly of 60S ribosomal subunits. Nucleic Acids Res. 2013;41:7889-904 pubmed publisher
    ..Thus, our data support a model in which Has1 helps to establish domain I architecture to prevent pre-rRNA turnover and couples domain I folding with consecutive pre-rRNA processing steps. ..
  4. Asano N, Kato K, Nakamura A, Komoda K, Tanaka I, Yao M. Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis. Nucleic Acids Res. 2015;43:4746-57 pubmed publisher
    Proteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation...
  5. Wan K, Kawara H, Yamamoto T, Kume K, Yabuki Y, Goshima T, et al. The essential function of Rrs1 in ribosome biogenesis is conserved in budding and fission yeasts. Yeast. 2015;32:607-14 pubmed publisher
    ..These results suggest that the essential function of Rrs1 in ribosome biogenesis may be conserved in budding and fission yeasts. ..