RPC31

Summary

Gene Symbol: RPC31
Description: DNA-directed RNA polymerase III subunit C31
Alias: ACP2, RPC8, DNA-directed RNA polymerase III subunit C31
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Chiannilkulchai N, Stalder R, Riva M, Carles C, Werner M, Sentenac A. RPC82 encodes the highly conserved, third-largest subunit of RNA polymerase C (III) from Saccharomyces cerevisiae. Mol Cell Biol. 1992;12:4433-40 pubmed
    ..Of eight RNA polymerase C genes tested, only the RPC31 gene on a multicopy plasmid was capable of suppressing the rpc82(Ts) defect, suggesting an interaction between the ..
  2. Hermann Le Denmat S, Werner M, Sentenac A, Thuriaux P. Suppression of yeast RNA polymerase III mutations by FHL1, a gene coding for a fork head protein involved in rRNA processing. Mol Cell Biol. 1994;14:2905-13 pubmed
    ..This accounts for the isolation of FHL1 as a dosage-dependent suppressor and suggests that rRNA processing depends on a still-unidentified RNA polymerase III transcript. ..
  3. Stettler S, Chiannilkulchai N, Hermann Le Denmat S, Lalo D, Lacroute F, Sentenac A, et al. A general suppressor of RNA polymerase I, II and III mutations in Saccharomyces cerevisiae. Mol Gen Genet. 1993;239:169-76 pubmed
    ..FL100) was screened for its ability to suppress conditionally defective mutations altering the 31 kDa subunit (rpc31-236) or the 53 kDa subunit (rpc53-254/424) of RNA polymerase III...
  4. Brun I, Sentenac A, Werner M. Dual role of the C34 subunit of RNA polymerase III in transcription initiation. EMBO J. 1997;16:5730-41 pubmed
  5. Rubbi L, Labarre Mariotte S, Chedin S, Thuriaux P. Functional characterization of ABC10alpha, an essential polypeptide shared by all three forms of eukaryotic DNA-dependent RNA polymerases. J Biol Chem. 1999;274:31485-92 pubmed
    ..This interaction is presumably a major rate-limiting step in assembly, since diploid cells containing only one functional gene copy for ABC10alpha have a partial growth defect. ..
  6. Vernekar D, Bhargava P. Yeast Bud27 modulates the biogenesis of Rpc128 and Rpc160 subunits and the assembly of RNA polymerase III. Biochim Biophys Acta. 2015;1849:1340-53 pubmed publisher
    ..Our results show that Bud27 is required in multiple activities responsible for pol III biogenesis and activity. ..
  7. Mosrin C, Riva M, Beltrame M, Cassar E, Sentenac A, Thuriaux P. The RPC31 gene of Saccharomyces cerevisiae encodes a subunit of RNA polymerase C (III) with an acidic tail. Mol Cell Biol. 1990;10:4737-43 pubmed
    The RPC31 gene encoding the C31 subunit of Saccharomyces cerevisiae RNA polymerase C (III) has been isolated, starting from a C-terminal fragment cloned on a lambda gt11 library...
  8. Thuillier V, Stettler S, Sentenac A, Thuriaux P, Werner M. A mutation in the C31 subunit of Saccharomyces cerevisiae RNA polymerase III affects transcription initiation. EMBO J. 1995;14:351-9 pubmed
    ..Interestingly, the C-terminal deletion was also suppressed by a high gene dosage of the DED1 gene encoding a putative helicase. ..
  9. Cieśla M, Towpik J, Graczyk D, Oficjalska Pham D, Harismendy O, Suleau A, et al. Maf1 is involved in coupling carbon metabolism to RNA polymerase III transcription. Mol Cell Biol. 2007;27:7693-702 pubmed
    ..Moreover, in cells grown in a nonfermentable carbon source, Maf1 regulates the levels of different tRNAs to various extents. The differences in regulation may contribute to the physiological role of Maf1. ..

More Information

Publications13

  1. Vannini A, Ringel R, Kusser A, Berninghausen O, Kassavetis G, Cramer P. Molecular basis of RNA polymerase III transcription repression by Maf1. Cell. 2010;143:59-70 pubmed publisher
    ..These results explain how Maf1 specifically represses transcription initiation from Pol III promoters and indicate that Maf1 also prevents reinitiation by binding Pol III during transcription elongation. ..
  2. Huet J, Riva M, Sentenac A, Fromageot P. Yeast RNA polymerase C and its subunits. Specific antibodies as structural and functional probes. J Biol Chem. 1985;260:15304-10 pubmed
    ..These results are discussed in terms of the participation of these polypeptides to the active enzyme molecule, and of their possible role in DNA binding or transcription factor recognition. ..
  3. Ferri M, Peyroche G, Siaut M, Lefebvre O, Carles C, Conesa C, et al. A novel subunit of yeast RNA polymerase III interacts with the TFIIB-related domain of TFIIIB70. Mol Cell Biol. 2000;20:488-95 pubmed
    ..The data indicate that C17 is a novel specific subunit of Pol III which participates together with C34 in the recruitment of Pol III by the preinitiation complex. ..
  4. Briand J, Navarro F, Rematier P, Boschiero C, Labarre S, Werner M, et al. Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III. Mol Cell Biol. 2001;21:6056-65 pubmed
    ..A ygr089-Delta null mutant has no detectable growth defect but aggravates the conditional growth defect of rpb8 mutants, suggesting that the interaction with Rpb8p may be physiologically relevant. ..