RPA43

Summary

Gene Symbol: RPA43
Description: DNA-directed RNA polymerase I subunit RPA43
Alias: DNA-directed RNA polymerase I subunit RPA43
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Milkereit P, Tschochner H. A specialized form of RNA polymerase I, essential for initiation and growth-dependent regulation of rRNA synthesis, is disrupted during transcription. EMBO J. 1998;17:3692-703 pubmed
    ..We propose that the formation and disruption of the pol I-Rrn3p complex reflects a molecular switch for regulating rRNA synthesis and its growth rate-dependent regulation. ..
  2. Albert B, Léger Silvestre I, Normand C, Ostermaier M, Pérez Fernández J, Panov K, et al. RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. J Cell Biol. 2011;192:277-93 pubmed publisher
    ..Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly. ..
  3. Kuhn C, Geiger S, Baumli S, Gartmann M, Gerber J, Jennebach S, et al. Functional architecture of RNA polymerase I. Cell. 2007;131:1260-72 pubmed
    ..In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming. ..
  4. Beckouët F, Labarre Mariotte S, Albert B, Imazawa Y, Werner M, Gadal O, et al. Two RNA polymerase I subunits control the binding and release of Rrn3 during transcription. Mol Cell Biol. 2008;28:1596-605 pubmed
    ..impaired its recruitment to the rDNA promoter, an effect that was bypassed by an N-terminal deletion of the Rpa43 subunit encoded by rpa43-35,326, and they strongly reduced the release of the Rrn3 initiation factor during ..
  5. Keener J, Josaitis C, Dodd J, Nomura M. Reconstitution of yeast RNA polymerase I transcription in vitro from purified components. TATA-binding protein is not required for basal transcription. J Biol Chem. 1998;273:33795-802 pubmed
    ..The role of TBP in pol I transcription is fundamentally different from its role in pol II or pol III transcription. ..
  6. Peyroche G, Milkereit P, Bischler N, Tschochner H, Schultz P, Sentenac A, et al. The recruitment of RNA polymerase I on rDNA is mediated by the interaction of the A43 subunit with Rrn3. EMBO J. 2000;19:5473-82 pubmed
    ..The existence of mammalian orthologues of A43 and Rrn3 suggests evolutionary conservation of the molecular mechanisms underlying rDNA transcription in eukaryotes. ..
  7. Harris B, Bose T, Lee K, Wang F, Lu S, Ross R, et al. Cohesion promotes nucleolar structure and function. Mol Biol Cell. 2014;25:337-46 pubmed publisher
    ..Our results strongly suggest that organization of the rDNA provided by cohesion is critical for formation and function of the nucleolus. ..
  8. Peyroche G, Levillain E, Siaut M, Callebaut I, Schultz P, Sentenac A, et al. The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits. Proc Natl Acad Sci U S A. 2002;99:14670-5 pubmed
    ..This hypothesis, combined with our structural data, suggests a new localization of Rpb7-Rpb4 subunits in the three-dimensional structure of yeast pol II. ..
  9. Zhou L, Le Roux G, Ducrot C, Chédin S, Labarre J, Riva M, et al. Repression of class I transcription by cadmium is mediated by the protein phosphatase 2A. Nucleic Acids Res. 2013;41:6087-97 pubmed publisher
    ..Taken together, our results point to a central role for the Pol I-Rrn3 complex as molecular switch for regulating Pol I transcription in response to toxic metals. ..

More Information

Publications22

  1. Stepanchick A, Zhi H, Cavanaugh A, Rothblum K, Schneider D, Rothblum L. DNA binding by the ribosomal DNA transcription factor rrn3 is essential for ribosomal DNA transcription. J Biol Chem. 2013;288:9135-44 pubmed publisher
    ..Control experiments demonstrated that these mutant Rrn3 constructs were capable of interacting with both rpa43 and SL1, two other activities demonstrated to be essential for Rrn3 function...
  2. Briand J, Navarro F, Rematier P, Boschiero C, Labarre S, Werner M, et al. Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III. Mol Cell Biol. 2001;21:6056-65 pubmed
    ..A ygr089-Delta null mutant has no detectable growth defect but aggravates the conditional growth defect of rpb8 mutants, suggesting that the interaction with Rpb8p may be physiologically relevant. ..
  3. Torreira E, Louro J, Pazos I, González Polo N, Gil Cartón D, Duran A, et al. The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription. elife. 2017;6: pubmed publisher
  4. Beckouët F, Mariotte Labarre S, Peyroche G, Nogi Y, Thuriaux P. Rpa43 and its partners in the yeast RNA polymerase I transcription complex. FEBS Lett. 2011;585:3355-9 pubmed publisher
    An Rpa43/Rpa14 stalk protrudes from RNA polymerase I (RNAPI), with homology to Rpb7/Rpb4 (RNAPII), Rpc25/Rpc17 (RNAPIII) and RpoE/RpoF (archaea)...
  5. Geiger S, Kuhn C, Leidig C, Renkawitz J, Cramer P. Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regions. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64:413-8 pubmed publisher
    ..This strategy should enable the crystallization of other proteins and subcomplexes with multiple flexibilities, as required for hybrid structure solution of large macromolecular assemblies. ..
  6. Gadal O, Labarre S, Boschiero C, Thuriaux P. Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system. EMBO J. 2002;21:5498-507 pubmed
    ..Conversely, rpa34-Delta mutants lacking Rpa34, a non-essential subunit associated with Rpa49, are lethal in top1-Delta but not in top3-Delta. ..
  7. Van Mullem V, Landrieux E, Vandenhaute J, Thuriaux P. Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Mol Microbiol. 2002;43:1105-13 pubmed
    ..Thus, the N-terminal zinc domain of Rpa12p determines its anchoring to RNA polymerase I and is the only critical part of that subunit in vivo. ..
  8. Fath S, Milkereit P, Podtelejnikov A, Bischler N, Schultz P, Bier M, et al. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. J Cell Biol. 2000;149:575-90 pubmed
    ..Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing. ..
  9. Schneider D, Nomura M. RNA polymerase I remains intact without subunit exchange through multiple rounds of transcription in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004;101:15112-7 pubmed
    ..Thus, Pol I is not a dynamic protein complex but rather a stable enzyme. ..
  10. Fernández Tornero C, Moreno Morcillo M, Rashid U, Taylor N, Ruiz F, Gruene T, et al. Crystal structure of the 14-subunit RNA polymerase I. Nature. 2013;502:644-9 pubmed publisher
    ..2 in RNA cleavage and Pol I insensitivity to ?-amanitin. The A49-A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2. ..
  11. Iacovella M, Golfieri C, Massari L, Busnelli S, Pagliuca C, Dal Maschio M, et al. Rio1 promotes rDNA stability and downregulates RNA polymerase I to ensure rDNA segregation. Nat Commun. 2015;6:6643 pubmed publisher
    ..During anaphase, Rio1 downregulates PolI by targeting its subunit Rpa43, causing PolI to dissociate from the rDNA...
  12. Engel K, French S, Viktorovskaya O, Beyer A, Schneider D. Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I. Mol Cell Biol. 2015;35:2321-31 pubmed publisher
    ..First, Spt6 physically associates with a Pol I subunit (Rpa43). Second, Spt6 interacts physically and genetically with Spt4/5, which directly affects Pol I transcription...
  13. Thuriaux P, Mariotte S, Buhler J, Sentenac A, Vu L, Lee B, et al. Gene RPA43 in Saccharomyces cerevisiae encodes an essential subunit of RNA polymerase I. J Biol Chem. 1995;270:24252-7 pubmed
    ..The corresponding gene, RPA43, encodes a 326-amino acid polypeptide matching the peptidic sequence of two tryptic fragments isolated from A43...