RPA190

Summary

Gene Symbol: RPA190
Description: DNA-directed RNA polymerase I core subunit RPA190
Alias: RRN1, DNA-directed RNA polymerase I core subunit RPA190
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Nogi Y, Yano R, Nomura M. Synthesis of large rRNAs by RNA polymerase II in mutants of Saccharomyces cerevisiae defective in RNA polymerase I. Proc Natl Acad Sci U S A. 1991;88:3962-6 pubmed
  2. Garcia Lopez M, Pelechano V, Mirón García M, Garrido Godino A, Garcia A, Calvo O, et al. The conserved foot domain of RNA pol II associates with proteins involved in transcriptional initiation and/or early elongation. Genetics. 2011;189:1235-48 pubmed publisher
  3. Keener J, Josaitis C, Dodd J, Nomura M. Reconstitution of yeast RNA polymerase I transcription in vitro from purified components. TATA-binding protein is not required for basal transcription. J Biol Chem. 1998;273:33795-802 pubmed
    ..The role of TBP in pol I transcription is fundamentally different from its role in pol II or pol III transcription. ..
  4. Flores A, Briand J, Gadal O, Andrau J, Rubbi L, Van Mullem V, et al. A protein-protein interaction map of yeast RNA polymerase III. Proc Natl Acad Sci U S A. 1999;96:7815-20 pubmed
    ..Together with parallel interaction studies based on dosage-dependent suppression of conditional mutants, our data suggest a model of the pol III preinitiation complex. ..
  5. Briand J, Navarro F, Rematier P, Boschiero C, Labarre S, Werner M, et al. Partners of Rpb8p, a small subunit shared by yeast RNA polymerases I, II and III. Mol Cell Biol. 2001;21:6056-65 pubmed
    ..I.KP.LW.GKQ) facing the GGLLM motif of Rpb8p. An RNA polymerase I mutant (rpa190-G728D) at the invariant glycyl of P.I.KP.LW.GKQ provokes a temperature-sensitive defect...
  6. Kuhn C, Geiger S, Baumli S, Gartmann M, Gerber J, Jennebach S, et al. Functional architecture of RNA polymerase I. Cell. 2007;131:1260-72 pubmed
    ..In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming. ..
  7. Wittekind M, Dodd J, Vu L, Kolb J, Buhler J, Sentenac A, et al. Isolation and characterization of temperature-sensitive mutations in RPA190, the gene encoding the largest subunit of RNA polymerase I from Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:3997-4008 pubmed
    ..A plasmid carrying RPA190, the gene encoding the largest subunit of the enzyme, was subjected to in vitro mutagenesis with hydroxylamine...
  8. Zaros C, Briand J, Boulard Y, Labarre Mariotte S, Garcia Lopez M, Thuriaux P, et al. Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases. Nucleic Acids Res. 2007;35:634-47 pubmed
  9. Soutourina J, Bordas Le Floch V, Gendrel G, Flores A, Ducrot C, Dumay Odelot H, et al. Rsc4 connects the chromatin remodeler RSC to RNA polymerases. Mol Cell Biol. 2006;26:4920-33 pubmed

More Information

Publications38

  1. Albert B, Léger Silvestre I, Normand C, Ostermaier M, Pérez Fernández J, Panov K, et al. RNA polymerase I-specific subunits promote polymerase clustering to enhance the rRNA gene transcription cycle. J Cell Biol. 2011;192:277-93 pubmed publisher
    ..Together our data suggest that localized rRNA production results in spatially constrained rRNA production, which is instrumental for nucleolar assembly. ..
  2. Huber A, Bodenmiller B, Uotila A, Stahl M, Wanka S, Gerrits B, et al. Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev. 2009;23:1929-43 pubmed publisher
    ..This demonstrates that Sch9 is a master regulator of protein synthesis. ..
  3. Van Mullem V, Landrieux E, Vandenhaute J, Thuriaux P. Rpa12p, a conserved RNA polymerase I subunit with two functional domains. Mol Microbiol. 2002;43:1105-13 pubmed
    ..Thus, the N-terminal zinc domain of Rpa12p determines its anchoring to RNA polymerase I and is the only critical part of that subunit in vivo. ..
  4. Schneider D, French S, Osheim Y, Bailey A, Vu L, Dodd J, et al. RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A. 2006;103:12707-12 pubmed
    ..These data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly. ..
  5. Yano R, Nomura M. Suppressor analysis of temperature-sensitive mutations of the largest subunit of RNA polymerase I in Saccharomyces cerevisiae: a suppressor gene encodes the second-largest subunit of RNA polymerase I. Mol Cell Biol. 1991;11:754-64 pubmed
    ..From these results, it is suggested that this putative zinc-binding domain is in physical proximity to and interacts with the putative zinc-binding domain of the A190 subunit. ..
  6. Guillen Ahlers H, Rao P, Levenstein M, Kennedy Darling J, Perumalla D, Jadhav A, et al. HyCCAPP as a tool to characterize promoter DNA-protein interactions in Saccharomyces cerevisiae. Genomics. 2016;107:267-73 pubmed publisher
  7. Engel K, French S, Viktorovskaya O, Beyer A, Schneider D. Spt6 Is Essential for rRNA Synthesis by RNA Polymerase I. Mol Cell Biol. 2015;35:2321-31 pubmed publisher
    ..The findings presented here identify an unexpected, essential role for Spt6 in synthesis of rRNA. ..
  8. McCusker J, Yamagishi M, Kolb J, Nomura M. Suppressor analysis of temperature-sensitive RNA polymerase I mutations in Saccharomyces cerevisiae: suppression of mutations in a zinc-binding motif by transposed mutant genes. Mol Cell Biol. 1991;11:746-53 pubmed
    Starting with two temperature-sensitive mutants (rpa190-1 and rpa190-5) of Saccharomyces cerevisiae, both of which are amino acid substitutions in the putative zinc-binding domain of the largest subunit (A190) of RNA polymerase I, we have ..
  9. Laribee R, Hosni Ahmed A, Workman J, Chen H. Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis. PLoS Genet. 2015;11:e1005113 pubmed publisher
    ..Collectively, these data demonstrate a novel role for Ccr4-Not in Pol I transcriptional regulation that is required for bridging mTORC1 signaling to ribosomal RNA synthesis. ..
  10. Viktorovskaya O, Appling F, Schneider D. Yeast transcription elongation factor Spt5 associates with RNA polymerase I and RNA polymerase II directly. J Biol Chem. 2011;286:18825-33 pubmed publisher
    ..Based on these data, we propose a model in which Spt5 is recruited to the rDNA early in transcription and propose that it plays an important role in ribosomal RNA synthesis through direct binding to the Pol I complex. ..
  11. Bouchoux C, Hautbergue G, Grenetier S, Carles C, Riva M, Goguel V. CTD kinase I is involved in RNA polymerase I transcription. Nucleic Acids Res. 2004;32:5851-60 pubmed
    ..The results suggest that CTDK-I might participate in the regulation of distinct nuclear transcriptional machineries, thus playing a role in the adaptation of the global transcriptional response to growth signalling. ..
  12. Schneider D, Nomura M. RNA polymerase I remains intact without subunit exchange through multiple rounds of transcription in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 2004;101:15112-7 pubmed
    ..Thus, Pol I is not a dynamic protein complex but rather a stable enzyme. ..
  13. Huang J, Moazed D. Association of the RENT complex with nontranscribed and coding regions of rDNA and a regional requirement for the replication fork block protein Fob1 in rDNA silencing. Genes Dev. 2003;17:2162-76 pubmed
    ..Furthermore, the Fob1-dependent associations of Net1 and Sir2 with the recombination hotspot region strongly suggest that Sir2 acts directly at this region to carry out its inhibitory effect on rDNA recombination and accelerated aging. ..
  14. Zhang Y, French S, Beyer A, Schneider D. The Transcription Factor THO Promotes Transcription Initiation and Elongation by RNA Polymerase I. J Biol Chem. 2016;291:3010-8 pubmed publisher
    ..Together, these data show that perturbation of THO function impairs transcription initiation and elongation by Pol I, identifying a new cellular target for the conserved THO complex. ..
  15. Harris B, Bose T, Lee K, Wang F, Lu S, Ross R, et al. Cohesion promotes nucleolar structure and function. Mol Biol Cell. 2014;25:337-46 pubmed publisher
    ..Our results strongly suggest that organization of the rDNA provided by cohesion is critical for formation and function of the nucleolus. ..
  16. Milkereit P, Tschochner H. A specialized form of RNA polymerase I, essential for initiation and growth-dependent regulation of rRNA synthesis, is disrupted during transcription. EMBO J. 1998;17:3692-703 pubmed
    ..We propose that the formation and disruption of the pol I-Rrn3p complex reflects a molecular switch for regulating rRNA synthesis and its growth rate-dependent regulation. ..
  17. Minaker S, Filiatrault M, Ben Aroya S, Hieter P, Stirling P. Biogenesis of RNA polymerases II and III requires the conserved GPN small GTPases in Saccharomyces cerevisiae. Genetics. 2013;193:853-64 pubmed publisher
    ..We propose that the three GPN proteins execute a common, and likely essential, function in RNA polymerase assembly and transport. ..
  18. Alonso B, Beraud C, Meguellati S, Chen S, Pellequer J, Armengaud J, et al. Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN. Cell Cycle. 2013;12:463-72 pubmed publisher
    ..These results suggest that all three GPN proteins act at the molecular level in sister chromatid cohesion mechanism as a GPN|GPN complex reminiscent of the homodimeric structure of PAB0955, an archaeal member of GPN-loop GTPase. ..
  19. Fernández Tornero C, Moreno Morcillo M, Rashid U, Taylor N, Ruiz F, Gruene T, et al. Crystal structure of the 14-subunit RNA polymerase I. Nature. 2013;502:644-9 pubmed publisher
    ..2 in RNA cleavage and Pol I insensitivity to ?-amanitin. The A49-A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2. ..
  20. Fath S, Milkereit P, Podtelejnikov A, Bischler N, Schultz P, Bier M, et al. Association of yeast RNA polymerase I with a nucleolar substructure active in rRNA synthesis and processing. J Cell Biol. 2000;149:575-90 pubmed
    ..Our results support the idea that a functional nucleolar subdomain formed independently of the state of rDNA transcription may serve as a scaffold for coordinated rRNA synthesis and processing. ..
  21. Cieśla M, Makała E, Płonka M, Bazan R, Gewartowski K, Dziembowski A, et al. Rbs1, a new protein implicated in RNA polymerase III biogenesis in yeast Saccharomyces cerevisiae. Mol Cell Biol. 2015;35:1169-81 pubmed publisher
    ..Additionally, Rbs1 interacts with the Crm1 exportin and shuttles between the cytoplasm and nucleus. We postulate that Rbs1 binds to the Pol III complex or subcomplex and facilitates its translocation to the nucleus. ..
  22. Nogi Y, Yano R, Dodd J, Carles C, Nomura M. Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures. Mol Cell Biol. 1993;13:114-22 pubmed
    ..In addition, the temperature-sensitive phenotype of the rpa12 null mutants can be partially suppressed by RPA190 (the gene for A190) on multicopy plasmids. These results suggest that A12...
  23. Tsang C, Zheng X. Opposing role of condensin and radiation-sensitive gene RAD52 in ribosomal DNA stability regulation. J Biol Chem. 2009;284:21908-19 pubmed publisher
    ..In contrast, deletion of RAD52 restores cell viability under the same conditions. These results reveal an opposing role of condensin and Rad52 in the control of rDNA stability under nutrient starvation conditions. ..
  24. Torreira E, Louro J, Pazos I, González Polo N, Gil Cartón D, Duran A, et al. The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription. elife. 2017;6: pubmed publisher
  25. Chen H, Fan M, Pfeffer L, Laribee R. The histone H3 lysine 56 acetylation pathway is regulated by target of rapamycin (TOR) signaling and functions directly in ribosomal RNA biogenesis. Nucleic Acids Res. 2012;40:6534-46 pubmed publisher
    ..Overall, these studies identify a novel chromatin regulatory role for TOR signaling and support a specific function for H3K56ac in ribosomal DNA (rDNA) gene transcription and nascent rRNA processing essential for cell growth. ..
  26. Leporé N, Lafontaine D. A functional interface at the rDNA connects rRNA synthesis, pre-rRNA processing and nucleolar surveillance in budding yeast. PLoS ONE. 2011;6:e24962 pubmed publisher
    ..We report in budding yeast that, in vivo, the interaction between the transcription elongation factor Spt5 and Rpa190, the largest subunit of RNA polymerase (Pol) I, requires the Spt5 C-terminal region (CTR), a conserved and highly ..
  27. Mirón García M, Garrido Godino A, García Molinero V, Hernández Torres F, Rodriguez Navarro S, Navarro F. The prefoldin bud27 mediates the assembly of the eukaryotic RNA polymerases in an rpb5-dependent manner. PLoS Genet. 2013;9:e1003297 pubmed publisher
    ..Finally, the role of URI seems to be conserved in humans, suggesting conserved mechanisms in RNA pols biogenesis. ..
  28. Shou W, Sakamoto K, Keener J, Morimoto K, Traverso E, Azzam R, et al. Net1 stimulates RNA polymerase I transcription and regulates nucleolar structure independently of controlling mitotic exit. Mol Cell. 2001;8:45-55 pubmed
    ..The independent functions of Net1 link a key event in the cell cycle to nucleolar processes that are fundamental to cell growth. ..
  29. Gerber J, Reiter A, Steinbauer R, Jakob S, Kuhn C, Cramer P, et al. Site specific phosphorylation of yeast RNA polymerase I. Nucleic Acids Res. 2008;36:793-802 pubmed
    ..2, suggesting a role in RNA cleavage/elongation or termination. Our results suggest that individual major or constitutively phosphorylated residues contribute to non-essential Pol I-functions. ..