Genomes and Genes
Gene Symbol: RPA12
Description: DNA-directed RNA polymerase I core subunit RPA12
Alias: RRN4, DNA-directed RNA polymerase I core subunit RPA12
Species: Saccharomyces cerevisiae S288c
- Kuhn C, Geiger S, Baumli S, Gartmann M, Gerber J, Jennebach S, et al. Functional architecture of RNA polymerase I. Cell. 2007;131:1260-72 pubmed..In contrast to Pol II, Pol I has a strong intrinsic 3'-RNA cleavage activity, which requires the C-terminal domain of subunit A12.2 and, apparently, enables ribosomal RNA proofreading and 3'-end trimming. ..
- Nogi Y, Yano R, Dodd J, Carles C, Nomura M. Gene RRN4 in Saccharomyces cerevisiae encodes the A12.2 subunit of RNA polymerase I and is essential only at high temperatures. Mol Cell Biol. 1993;13:114-22 pubmed..Thus, A12.2 is a homolog of B12.6. We propose to rename the RRN4 gene RPA12. Deletion of RPA12 produces cells that are heat but not cold sensitive for growth...
- Catala M, Tremblay M, Samson E, Conconi A, Abou Elela S. Deletion of Rnt1p alters the proportion of open versus closed rRNA gene repeats in yeast. Mol Cell Biol. 2008;28:619-29 pubmed..The results demonstrate that defects in pre-rRNA processing can influence the chromatin structure of the rRNA gene arrays and reveal links among the rRNA gene chromatin, transcription, and processing. ..
- Schneider D, French S, Osheim Y, Bailey A, Vu L, Dodd J, et al. RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A. 2006;103:12707-12 pubmed..These data suggest that Spt4p, Spt5p, and, potentially, other regulators of Pol I transcription elongation play important roles in coupling rRNA transcription to its processing and ribosome assembly. ..
- Fernández Tornero C, Moreno Morcillo M, Rashid U, Taylor N, Ruiz F, Gruene T, et al. Crystal structure of the 14-subunit RNA polymerase I. Nature. 2013;502:644-9 pubmed publisher..2 in RNA cleavage and Pol I insensitivity to ?-amanitin. The A49-A34.5 heterodimer embraces subunit A135 through extended arms, thereby contacting and potentially regulating subunit A12.2. ..
- Harris B, Bose T, Lee K, Wang F, Lu S, Ross R, et al. Cohesion promotes nucleolar structure and function. Mol Biol Cell. 2014;25:337-46 pubmed publisher..Our results strongly suggest that organization of the rDNA provided by cohesion is critical for formation and function of the nucleolus. ..
- Laribee R, Hosni Ahmed A, Workman J, Chen H. Ccr4-not regulates RNA polymerase I transcription and couples nutrient signaling to the control of ribosomal RNA biogenesis. PLoS Genet. 2015;11:e1005113 pubmed publisher..Furthermore, ccr4Î” is synthetically sick when paired with rpa12Î” and the double mutant has enhanced sensitivity to transcription elongation inhibition suggesting that Ccr4-..
- Nogi Y, Vu L, Nomura M. An approach for isolation of mutants defective in 35S ribosomal RNA synthesis in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1991;88:7026-30 pubmed..We have thus isolated many mutants and identified at least nine genes (RRN1-RRN9) involved in 35S rRNA synthesis, two of which correspond to known RNA polymerase I subunit genes RPA190 and RPA135. ..
- Gerber J, Reiter A, Steinbauer R, Jakob S, Kuhn C, Cramer P, et al. Site specific phosphorylation of yeast RNA polymerase I. Nucleic Acids Res. 2008;36:793-802 pubmed..2, suggesting a role in RNA cleavage/elongation or termination. Our results suggest that individual major or constitutively phosphorylated residues contribute to non-essential Pol I-functions. ..
- Keener J, Josaitis C, Dodd J, Nomura M. Reconstitution of yeast RNA polymerase I transcription in vitro from purified components. TATA-binding protein is not required for basal transcription. J Biol Chem. 1998;273:33795-802 pubmed..The role of TBP in pol I transcription is fundamentally different from its role in pol II or pol III transcription. ..