RNT1

Summary

Gene Symbol: RNT1
Description: ribonuclease III
Alias: ribonuclease III
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Liang Y, Lavoie M, Comeau M, Abou Elela S, Ji X. Structure of a eukaryotic RNase III postcleavage complex reveals a double-ruler mechanism for substrate selection. Mol Cell. 2014;54:431-44 pubmed publisher
    b>Ribonuclease III (RNase III) enzymes are a family of double-stranded RNA (dsRNA)-specific endoribonucleases required for RNA maturation and gene regulation...
  2. Ghazal G, Ge D, Gervais Bird J, Gagnon J, Abou Elela S. Genome-wide prediction and analysis of yeast RNase III-dependent snoRNA processing signals. Mol Cell Biol. 2005;25:2981-94 pubmed
    ..The results presented here reveal a new role of yeast RNase III in the processing of intron-encoded snoRNAs that permits independent regulation of the host mRNA and its associated snoRNA. ..
  3. Rondon A, Mischo H, Kawauchi J, Proudfoot N. Fail-safe transcriptional termination for protein-coding genes in S. cerevisiae. Mol Cell. 2009;36:88-98 pubmed publisher
    ..The other termination mechanism is mediated by Rnt1 cleavage of the nascent transcript...
  4. Ge D, Lamontagne B, Elela S. RNase III-mediated silencing of a glucose-dependent repressor in yeast. Curr Biol. 2005;15:140-5 pubmed
    ..In vitro, Rnt1p cleaved Mig2p mRNA and a silent mutation that disrupts Rnt1p signals blocked Mig2p mRNA degradation. These observations reveal a new RNase III-dependent mechanism of eukaryotic mRNA degradation. ..
  5. Catala M, Tremblay M, Samson E, Conconi A, Abou Elela S. Deletion of Rnt1p alters the proportion of open versus closed rRNA gene repeats in yeast. Mol Cell Biol. 2008;28:619-29 pubmed
    ..The results demonstrate that defects in pre-rRNA processing can influence the chromatin structure of the rRNA gene arrays and reveal links among the rRNA gene chromatin, transcription, and processing. ..
  6. Lavoie M, Abou Elela S. Yeast ribonuclease III uses a network of multiple hydrogen bonds for RNA binding and cleavage. Biochemistry. 2008;47:8514-26 pubmed publisher
    ..One mode is primarily mediated by the dsRNA binding domain and leads to the formation of stable RNA/protein complex, while the other requires the presence of the nuclease and N-terminal domains and leads to RNA cleavage. ..
  7. Chanfreau G, Elela S, Ares M, Guthrie C. Alternative 3'-end processing of U5 snRNA by RNase III. Genes Dev. 1997;11:2741-51 pubmed
    ..A temperature-sensitive mutation in the RNT1 gene encoding RNase III blocks accumulation of U5L in vivo...
  8. Henras A, Sam M, Hiley S, Wu H, Hughes T, Feigon J, et al. Biochemical and genomic analysis of substrate recognition by the double-stranded RNA binding domain of yeast RNase III. RNA. 2005;11:1225-37 pubmed
    ..This study underscores the importance of a few amino acid contacts for positioning of a dsRBD onto its RNA target, and implicates the specific orientation of helix 1 on the RNA for proper positioning of the catalytic domain. ..
  9. Vasiljeva L, Buratowski S. Nrd1 interacts with the nuclear exosome for 3' processing of RNA polymerase II transcripts. Mol Cell. 2006;21:239-48 pubmed
    ..Since Nrd1 is known to bind RNA polymerase II and be important for sn/snoRNA 3' end processing, Nrd1 may link transcription and RNA 3' end formation with surveillance by the exosome. ..

More Information

Publications35

  1. Lamontagne B, Abou Elela S. Short RNA guides cleavage by eukaryotic RNase III. PLoS ONE. 2007;2:e472 pubmed
  2. Catala M, Lamontagne B, Larose S, Ghazal G, Elela S. Cell cycle-dependent nuclear localization of yeast RNase III is required for efficient cell division. Mol Biol Cell. 2004;15:3015-30 pubmed
    Members of the double-stranded RNA-specific ribonuclease III (RNase III) family were shown to affect cell division and chromosome segregation, presumably through an RNA interference-dependent mechanism...
  3. Ghazal G, Gagnon J, Jacques P, Landry J, Robert F, Elela S. Yeast RNase III triggers polyadenylation-independent transcription termination. Mol Cell. 2009;36:99-109 pubmed publisher
    ..Here we show that the yeast ortholog of the dsRNA-specific ribonuclease III (Rnt1p) may trigger Rat1p-dependent termination of RNA transcripts that fail to terminate near ..
  4. Lebars I, Lamontagne B, Yoshizawa S, Aboul Elela S, Fourmy D. Solution structure of conserved AGNN tetraloops: insights into Rnt1p RNA processing. EMBO J. 2001;20:7250-8 pubmed
    ..These results indicate that yeast RNase III recognizes the fold of a conserved single-stranded tetraloop to direct specific dsRNA cleavage. ..
  5. Catala M, Aksouh L, Abou Elela S. RNA-dependent regulation of the cell wall stress response. Nucleic Acids Res. 2012;40:7507-17 pubmed publisher
    ..More specifically, the deletion of the yeast nuclear dsRNA-specific ribonuclease III (Rnt1p) increased the expression of the mRNAs associated with both the morphogenesis checkpoint and the cell ..
  6. Allmang C, Kufel J, Chanfreau G, Mitchell P, Petfalski E, Tollervey D. Functions of the exosome in rRNA, snoRNA and snRNA synthesis. EMBO J. 1999;18:5399-410 pubmed
    ..We conclude that the exosome is involved in the processing of many RNA substrates and that different components can have distinct functions. ..
  7. Egecioglu D, Henras A, Chanfreau G. Contributions of Trf4p- and Trf5p-dependent polyadenylation to the processing and degradative functions of the yeast nuclear exosome. RNA. 2006;12:26-32 pubmed
    ..These results suggest that polyadenylation of RNA processing intermediates plays a functional role in RNA processing pathways and is not limited to RNA surveillance functions. ..
  8. Larose S, Laterreur N, Ghazal G, Gagnon J, Wellinger R, Elela S. RNase III-dependent regulation of yeast telomerase. J Biol Chem. 2007;282:4373-81 pubmed
    ..Deletion or inactivation of RNT1 induced the expression of Est1, Est2, Est3, and Tlc1 RNAs and increased telomerase activity, leading to elongation ..
  9. Lee A, Henras A, Chanfreau G. Multiple RNA surveillance pathways limit aberrant expression of iron uptake mRNAs and prevent iron toxicity in S. cerevisiae. Mol Cell. 2005;19:39-51 pubmed
  10. Ursic D, Chinchilla K, Finkel J, Culbertson M. Multiple protein/protein and protein/RNA interactions suggest roles for yeast DNA/RNA helicase Sen1p in transcription, transcription-coupled DNA repair and RNA processing. Nucleic Acids Res. 2004;32:2441-52 pubmed
    ..The protein-protein and protein-RNA interactions reported here suggest that the DNA/RNA helicase activity of Sen1p is utilized for several different purposes in multiple gene expression pathways. ..
  11. Zer C, Chanfreau G. Regulation and surveillance of normal and 3'-extended forms of the yeast aci-reductone dioxygenase mRNA by RNase III cleavage and exonucleolytic degradation. J Biol Chem. 2005;280:28997-9003 pubmed
  12. Gagnon J, Lavoie M, Catala M, Malenfant F, Elela S. Transcriptome wide annotation of eukaryotic RNase III reactivity and degradation signals. PLoS Genet. 2015;11:e1005000 pubmed publisher
    ..genomic and biochemical annotations of the signals required for RNA degradation by the dsRNA specific ribonuclease III (Rnt1p) and examine its impact on transcriptome expression...
  13. Giorgi C, Fatica A, Nagel R, Bozzoni I. Release of U18 snoRNA from its host intron requires interaction of Nop1p with the Rnt1p endonuclease. EMBO J. 2001;20:6856-65 pubmed
    ..These data indicate that cleavage of U18, snR38 and possibly other intron-encoded snoRNAs is a regulated process, since the stem is cleaved by the Rnt1p endonuclease only when snoRNP assembly has occurred. ..
  14. Chinchilla K, RodrĂ­guez Molina J, Ursic D, Finkel J, Ansari A, Culbertson M. Interactions of Sen1, Nrd1, and Nab3 with multiple phosphorylated forms of the Rpb1 C-terminal domain in Saccharomyces cerevisiae. Eukaryot Cell. 2012;11:417-29 pubmed publisher
  15. Lamontagne B, Tremblay A, Abou Elela S. The N-terminal domain that distinguishes yeast from bacterial RNase III contains a dimerization signal required for efficient double-stranded RNA cleavage. Mol Cell Biol. 2000;20:1104-15 pubmed
    Yeast Rnt1 is a member of the double-stranded RNA (dsRNA)-specific RNase III family identified by conserved dsRNA binding (dsRBD) and nuclease domains...
  16. Leulliot N, Quevillon Cheruel S, Graille M, van Tilbeurgh H, Leeper T, Godin K, et al. A new alpha-helical extension promotes RNA binding by the dsRBD of Rnt1p RNAse III. EMBO J. 2004;23:2468-77 pubmed
    b>Rnt1 endoribonuclease, the yeast homolog of RNAse III, plays an important role in the maturation of a diverse set of RNAs...
  17. Hartman E, Wang Z, Zhang Q, Roy K, Chanfreau G, Feigon J. Intrinsic dynamics of an extended hydrophobic core in the S. cerevisiae RNase III dsRBD contributes to recognition of specific RNA binding sites. J Mol Biol. 2013;425:546-62 pubmed publisher
    ..These results reveal that dynamics in the extended hydrophobic core are important for binding site selection by the Rnt1p dsRBD. ..
  18. Singh V, Azad G, Sariki S, Tomar R. Flocculation in Saccharomyces cerevisiae is regulated by RNA/DNA helicase Sen1p. FEBS Lett. 2015;589:3165-74 pubmed publisher
    ..Furthermore, a deletion mutant of the RNA processing machinery (RNT1), and SEN1 mutants that are unable to interact with Rnt1p, exhibit a flocculation phenotype...
  19. Yang P, Rotondo G, Porras T, Legrain P, Chanfreau G. The Shq1p.Naf1p complex is required for box H/ACA small nucleolar ribonucleoprotein particle biogenesis. J Biol Chem. 2002;277:45235-42 pubmed
    ..Shq1p and Naf1p form a complex, but they are not strongly associated with box H/ACA snoRNPs. We propose that Shq1p and Naf1p are involved in the early biogenesis steps of box H/ACA snoRNP assembly. ..
  20. Tremblay A, Lamontagne B, Catala M, Yam Y, Larose S, Good L, et al. A physical interaction between Gar1p and Rnt1pi is required for the nuclear import of H/ACA small nucleolar RNA-associated proteins. Mol Cell Biol. 2002;22:4792-802 pubmed
    ..These results demonstrate colocalization of various components of the rRNA maturation complex and suggest a mechanism that links rRNA pseudouridylation and cleavage factors. ..
  21. Biver S, Portetelle D, Vandenbol M. Multicopy suppression screen in a Saccharomyces cerevisiae strain lacking the Rab GTPase-activating protein Msb3p. Biotechnol Lett. 2011;33:123-9 pubmed publisher
    ..Three genes (ADH1, RNT1, and SUI1) were found to suppress the CdCl(2) sensitivity of the msb3 strain and three others (YAP6, ZEO1, and SLM1)..
  22. Faber A, Vos J, Vos H, Ghazal G, Elela S, Raué H. The RNA catabolic enzymes Rex4p, Rnt1p, and Dbr1p show genetic interaction with trans-acting factors involved in processing of ITS1 in Saccharomyces cerevisiae pre-rRNA. RNA. 2004;10:1946-56 pubmed
    ..Moreover, inactivation of the DBR1 gene in rrp2-1, or the RNT1 gene in rrp5-Delta3 mutant cells also negates the effects of the original mutation on pre-rRNA processing...
  23. Roy K, Chanfreau G. Stress-induced nuclear RNA degradation pathways regulate yeast bromodomain factor 2 to promote cell survival. PLoS Genet. 2014;10:e1004661 pubmed publisher
  24. Sam M, Henras A, Chanfreau G. A conserved major groove antideterminant for Saccharomyces cerevisiae RNase III recognition. Biochemistry. 2005;44:4181-7 pubmed
    ..cerevisiae RNase III activity, and suggest a rationale for their apparent counter selection in RNA processing sites. ..
  25. Li W, Selvam K, Rahman S, Li S. Sen1, the yeast homolog of human senataxin, plays a more direct role than Rad26 in transcription coupled DNA repair. Nucleic Acids Res. 2016;44:6794-802 pubmed publisher
    ..Our results indicate that Sen1 plays a more direct role than Rad26 in TCR. ..
  26. Song H, Fang X, Jin L, Shaw G, Wang Y, Ji X. The Functional Cycle of Rnt1p: Five Consecutive Steps of Double-Stranded RNA Processing by a Eukaryotic RNase III. Structure. 2017;25:353-363 pubmed publisher
    ..Overall, our results indicate that substrate selection is achieved independent of cleavage, allowing the recognition of substrates with different structures while preserving the basic mechanism of cleavage. ..