Gene Symbol: RMI1
Description: Rmi1p
Alias: NCE4, Rmi1p
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Mullen J, Nallaseth F, Lan Y, Slagle C, Brill S. Yeast Rmi1/Nce4 controls genome stability as a subunit of the Sgs1-Top3 complex. Mol Cell Biol. 2005;25:4476-87 pubmed
    ..RMI1/NCE4 was identified as a potential intermediate in the SGS1-TOP3 pathway, based on the observation that strains lacking ..
  2. Chang M, Bellaoui M, Zhang C, Desai R, Morozov P, Delgado Cruzata L, et al. RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. EMBO J. 2005;24:2024-33 pubmed
    ..revealed that the genetic interaction profile of the gene RMI1 (RecQ-mediated genome instability, also known as NCE4 and YPL024W) was highly similar to that of SGS1 and TOP3, suggesting a functional relationship between Rmi1 and the ..
  3. Chen C, Brill S. Binding and activation of DNA topoisomerase III by the Rmi1 subunit. J Biol Chem. 2007;282:28971-9 pubmed
    b>Rmi1 is a conserved oligonucleotide and oligosaccharide binding-fold protein that is associated with RecQ DNA helicase complexes from humans (BLM-TOP3 alpha) and yeast (Sgs1-Top3)...
  4. Bonner J, Choi K, Xue X, Torres N, Szakal B, Wei L, et al. Smc5/6 Mediated Sumoylation of the Sgs1-Top3-Rmi1 Complex Promotes Removal of Recombination Intermediates. Cell Rep. 2016;16:368-378 pubmed publisher
    ..The DNA helicase-topoisomerase complex, Sgs1-Top3-Rmi1 (STR), is the major pathway for processing these intermediates to generate conservative products...
  5. Tang S, Wu M, Zhang R, Hunter N. Pervasive and essential roles of the Top3-Rmi1 decatenase orchestrate recombination and facilitate chromosome segregation in meiosis. Mol Cell. 2015;57:607-21 pubmed publisher
    ..Sgs1 can associate with type-I topoisomerase Top3 and its accessory factor Rmi1 to form a conserved complex best known for its unique ability to decatenate double-Holliday junctions...
  6. Cejka P, Cannavo E, Polaczek P, Masuda Sasa T, Pokharel S, Campbell J, et al. DNA end resection by Dna2-Sgs1-RPA and its stimulation by Top3-Rmi1 and Mre11-Rad50-Xrs2. Nature. 2010;467:112-6 pubmed publisher
    ..In addition to this core machinery, we establish that both the topoisomerase 3 (Top3) and Rmi1 complex and the Mre11-Rad50-Xrs2 complex (MRX) have important roles as stimulatory components...
  7. Chen C, Brill S. An essential DNA strand-exchange activity is conserved in the divergent N-termini of BLM orthologs. EMBO J. 2010;29:1713-25 pubmed publisher
    ..is highly conserved and all BLM orthologs, including budding yeast Sgs1, have a large N-terminus that binds Top3-Rmi1 but has no known catalytic activity...
  8. Lai M, Seki M, Ui A, Enomoto T. Rmi1, a member of the Sgs1-Top3 complex in budding yeast, contributes to sister chromatid cohesion. EMBO Rep. 2007;8:685-90 pubmed
    The Saccharomyces cerevisiae RecQ-mediated genome instability (Rmi1) protein was recently identified as the third member of the slow growth suppressor 1-DNA topoisomerase III (Sgs1-Top3) complex, which is required for maintaining genomic ..
  9. Cejka P, Plank J, Dombrowski C, Kowalczykowski S. Decatenation of DNA by the S. cerevisiae Sgs1-Top3-Rmi1 and RPA complex: a mechanism for disentangling chromosomes. Mol Cell. 2012;47:886-96 pubmed publisher
    Genetic evidence indicates that Saccharomyces cerevisiae Sgs1, Top3, and Rmi1 resolve topologically linked intermediates arising from DNA replication and recombination...

More Information


  1. Lai M, Seki M, Tada S, Enomoto T. Rmi1 functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1. Biochem Biophys Res Commun. 2012;427:682-6 pubmed publisher
    ..cerevisiae RecQ helicase (Sgs1) combines with DNA topoisomerase III (Top3) and RecQ-mediated genome instability 1 (Rmi1) to form an evolutionarily conserved complex that is required for processing homologous recombination intermediates ..
  2. Bernstein K, Juanchich A, Sunjevaric I, Rothstein R. The Shu complex regulates Rad52 localization during rDNA repair. DNA Repair (Amst). 2013;12:786-90 pubmed publisher
    ..Our results suggest that in the absence of UAF30, the Shu complex plays a central role in Rad52 rDNA localization as long as Rad52 can be sumoylated. This discrimination is important for rDNA copy number homeostasis. ..
  3. Fasching C, Cejka P, Kowalczykowski S, Heyer W. Top3-Rmi1 dissolve Rad51-mediated D loops by a topoisomerase-based mechanism. Mol Cell. 2015;57:595-606 pubmed publisher
    ..Also, the human Topoisomerase IIIa-RMI1-RMI2 complex is capable of dissolving D loops...
  4. Bernstein K, Shor E, Sunjevaric I, Fumasoni M, Burgess R, Foiani M, et al. Sgs1 function in the repair of DNA replication intermediates is separable from its role in homologous recombinational repair. EMBO J. 2009;28:915-25 pubmed publisher
    ..The budding yeast Saccharomyces cerevisiae has one RecQ helicase, Sgs1, which functions with Top3 and Rmi1 in DNA repair...
  5. Smith J, Chen Q, Yatsunyk L, Nicoludis J, Garcia M, Kranaster R, et al. Rudimentary G-quadruplex-based telomere capping in Saccharomyces cerevisiae. Nat Struct Mol Biol. 2011;18:478-85 pubmed publisher
    ..These findings demonstrate that, at least in the absence of full natural capping, G4 DNA can play a positive role at telomeres in vivo. ..
  6. Ii M, Ii T, Mironova L, Brill S. Epistasis analysis between homologous recombination genes in Saccharomyces cerevisiae identifies multiple repair pathways for Sgs1, Mus81-Mms4 and RNase H2. Mutat Res. 2011;714:33-43 pubmed publisher
    ..We conclude that SGS1 and MUS81 act in parallel pathways downstream of RAD51 and RAD52, respectively. The data further indicate these pathways share common components and display complex interactions. ..
  7. Alzu A, Bermejo R, Begnis M, Lucca C, Piccini D, Carotenuto W, et al. Senataxin associates with replication forks to protect fork integrity across RNA-polymerase-II-transcribed genes. Cell. 2012;151:835-846 pubmed publisher
  8. Ashton T, Mankouri H, Heidenblut A, McHugh P, Hickson I. Pathways for Holliday junction processing during homologous recombination in Saccharomyces cerevisiae. Mol Cell Biol. 2011;31:1921-33 pubmed publisher
    The Saccharomyces cerevisiae Rmi1 protein is a component of the highly conserved Sgs1-Top3-Rmi1 complex...
  9. Glineburg M, Chavez A, Agrawal V, Brill S, Johnson F. Resolution by unassisted Top3 points to template switch recombination intermediates during DNA replication. J Biol Chem. 2013;288:33193-204 pubmed publisher
    The evolutionarily conserved Sgs1/Top3/Rmi1 (STR) complex plays vital roles in DNA replication and repair...
  10. Kaur H, De Muyt A, Lichten M. Top3-Rmi1 DNA single-strand decatenase is integral to the formation and resolution of meiotic recombination intermediates. Mol Cell. 2015;57:583-594 pubmed publisher
    The topoisomerase III (Top3)-Rmi1 heterodimer, which catalyzes DNA single-strand passage, forms a conserved complex with the Bloom's helicase (BLM, Sgs1 in budding yeast)...
  11. Mankouri H, Ngo H, Hickson I. Shu proteins promote the formation of homologous recombination intermediates that are processed by Sgs1-Rmi1-Top3. Mol Biol Cell. 2007;18:4062-73 pubmed
    ..Similar findings were also observed in shu1 cells in which Rmi1 or Top3 function was impaired...
  12. Menolfi D, Delamarre A, Lengronne A, Pasero P, Branzei D. Essential Roles of the Smc5/6 Complex in Replication through Natural Pausing Sites and Endogenous DNA Damage Tolerance. Mol Cell. 2015;60:835-46 pubmed publisher