RLP24

Summary

Gene Symbol: RLP24
Description: ATPase-activating ribosome biosynthesis protein
Alias: ATPase-activating ribosome biosynthesis protein
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Lo K, Li Z, Bussiere C, Bresson S, Marcotte E, Johnson A. Defining the pathway of cytoplasmic maturation of the 60S ribosomal subunit. Mol Cell. 2010;39:196-208 pubmed publisher
    ..Finally, the release of Tif6 is a prerequisite for the release of the nuclear export adaptor Nmd3. Establishing this pathway provides an important conceptual framework for understanding ribosome maturation. ..
  2. Harnpicharnchai P, Jakovljevic J, Horsey E, Miles T, Roman J, Rout M, et al. Composition and functional characterization of yeast 66S ribosome assembly intermediates. Mol Cell. 2001;8:505-15 pubmed
    ..These results demonstrate that proteomic approaches in concert with genetic tools provide powerful means to purify and characterize ribosome assembly intermediates. ..
  3. Lo K, Li Z, Wang F, Marcotte E, Johnson A. Ribosome stalk assembly requires the dual-specificity phosphatase Yvh1 for the exchange of Mrt4 with P0. J Cell Biol. 2009;186:849-62 pubmed publisher
    ..The initial assembly of the ribosome with Mrt4 may provide functional compartmentalization of ribosome assembly in addition to the spatial separation afforded by the nuclear envelope. ..
  4. Saveanu C, Bienvenu D, Namane A, Gleizes P, Gas N, Jacquier A, et al. Nog2p, a putative GTPase associated with pre-60S subunits and required for late 60S maturation steps. EMBO J. 2001;20:6475-84 pubmed
    ..These results suggest that transient, possibly GTP-dependent association of Nog2p with the pre-ribosomes might trigger late rRNA maturation steps in ribosomal large subunit biogenesis. ..
  5. Honma Y, Kitamura A, Shioda R, Maruyama H, Ozaki K, Oda Y, et al. TOR regulates late steps of ribosome maturation in the nucleoplasm via Nog1 in response to nutrients. EMBO J. 2006;25:3832-42 pubmed
    ..Nog1 formed a complex that included 60S ribosomal proteins and pre-ribosomal proteins Nop7 and Rlp24. The Nog1 complex shuttled between the nucleolus and the nucleoplasm for ribosome biogenesis, but it was tethered ..
  6. Pertschy B, Saveanu C, Zisser G, Lebreton A, Tengg M, Jacquier A, et al. Cytoplasmic recycling of 60S preribosomal factors depends on the AAA protein Drg1. Mol Cell Biol. 2007;27:6581-92 pubmed
    ..inactivation of Drg1 leads to an increased cytoplasmic localization of shuttling pre-60S maturation factors like Rlp24, Arx1, and Tif6...
  7. Lebreton A, Saveanu C, Decourty L, Jacquier A, Fromont Racine M. Nsa2 is an unstable, conserved factor required for the maturation of 27 SB pre-rRNAs. J Biol Chem. 2006;281:27099-108 pubmed
    ..Indeed, when ribosome biogenesis was blocked upstream of Nsa2, this short half-lived protein was largely depleted, suggesting that its cellular levels are tightly regulated. ..
  8. Yao Y, Demoinet E, Saveanu C, Lenormand P, Jacquier A, Fromont Racine M. Ecm1 is a new pre-ribosomal factor involved in pre-60S particle export. RNA. 2010;16:1007-17 pubmed publisher
    ..These data highlight the involvement of many factors acting together to export pre-60S particles. ..
  9. Bussiere C, Hashem Y, Arora S, Frank J, Johnson A. Integrity of the P-site is probed during maturation of the 60S ribosomal subunit. J Cell Biol. 2012;197:747-59 pubmed publisher
    ..We propose that Efl1 promotes a functional check of the integrity of the 60S subunit before its first round of translation. ..

More Information

Publications16

  1. Parnell K, Bass B. Functional redundancy of yeast proteins Reh1 and Rei1 in cytoplasmic 60S subunit maturation. Mol Cell Biol. 2009;29:4014-23 pubmed publisher
    ..We observe that strains lacking both Reh1 and Rei1 accumulate salt-labile 60S subunits, suggesting that Reh1/Rei1 is necessary for the cytoplasmic 60S subunit to adopt its mature, stable form. ..
  2. Loibl M, Klein I, Prattes M, Schmidt C, Kappel L, Zisser G, et al. The drug diazaborine blocks ribosome biogenesis by inhibiting the AAA-ATPase Drg1. J Biol Chem. 2014;289:3913-22 pubmed publisher
    ..As a consequence the physiological activity of Drg1, i.e. the release of Rlp24 from pre-60S particles, is blocked, and further progression of cytoplasmic preribosome maturation is prevented...
  3. Fuentes J, Datta K, Sullivan S, Walker A, Maddock J. In vivo functional characterization of the Saccharomyces cerevisiae 60S biogenesis GTPase Nog1. Mol Genet Genomics. 2007;278:105-23 pubmed
    ..Several conserved charge-to-alanine substitutions outside the GTPase domain display modest growth phenotypes indicating that these residues are not critical for function. ..
  4. Kappel L, Loibl M, Zisser G, Klein I, Fruhmann G, Gruber C, et al. Rlp24 activates the AAA-ATPase Drg1 to initiate cytoplasmic pre-60S maturation. J Cell Biol. 2012;199:771-82 pubmed publisher
    ..However, its exact role in ribosome biogenesis has been unknown. Here we show that the shuttling protein Rlp24 recruited Drg1 to pre-60S particles and stimulated its ATPase activity...
  5. Talkish J, Zhang J, Jakovljevic J, Horsey E, Woolford J. Hierarchical recruitment into nascent ribosomes of assembly factors required for 27SB pre-rRNA processing in Saccharomyces cerevisiae. Nucleic Acids Res. 2012;40:8646-61 pubmed
    ..Inhibition of TOR kinase decreases association of Rpf2, Spb4, Nog1 and Nog2 with pre-ribosomes. ..
  6. Occhipinti L, Chang Y, Altvater M, Menet A, Kemmler S, Panse V. Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2. Nucleic Acids Res. 2013;41:8266-79 pubmed publisher
    ..We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel. ..
  7. Lebreton A, Saveanu C, Decourty L, Rain J, Jacquier A, Fromont Racine M. A functional network involved in the recycling of nucleocytoplasmic pre-60S factors. J Cell Biol. 2006;173:349-60 pubmed
    ..We propose a model in which Rei1 is a key factor for the coordinated dissociation and recycling of the last pre-60S factors before newly synthesized large ribosomal subunits enter translation. ..