Gene Symbol: RIP1
Description: ubiquinol--cytochrome-c reductase catalytic subunit RIP1
Alias: ubiquinol--cytochrome-c reductase catalytic subunit RIP1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Hunte C, Koepke J, Lange C, Rossmanith T, Michel H. Structure at 2.3 A resolution of the cytochrome bc(1) complex from the yeast Saccharomyces cerevisiae co-crystallized with an antibody Fv fragment. Structure. 2000;8:669-84 pubmed
    ..Each 'monomer' of the refined model includes 2178 amino acid residues of subunits COR1, QCR2, COB, CYT1, RIP1, QCR6, QCR7, QCR8 and QCR9 of the cytochrome bc(1) complex and of the polypeptides V(H) and V(L) of the Fv fragment,..
  2. Mick D, Wagner K, van der Laan M, Frazier A, Perschil I, Pawlas M, et al. Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly. EMBO J. 2007;26:4347-58 pubmed
    ..We suggest that Shy1 links Cox1 translational regulation to complex IV assembly and supercomplex formation. ..
  3. Mokranjac D, Sichting M, Popov Celeketic D, Mapa K, Gevorkyan Airapetov L, Zohary K, et al. Role of Tim50 in the transfer of precursor proteins from the outer to the inner membrane of mitochondria. Mol Biol Cell. 2009;20:1400-7 pubmed publisher
    ..This function of Tim50 depends on its association with TIM23. We conclude that the efficient transfer of precursors between TOM and TIM23 complexes requires the concerted action of Tim50 with Tim23. ..
  4. Hildenbeutel M, Hegg E, Stephan K, Gruschke S, Meunier B, Ott M. Assembly factors monitor sequential hemylation of cytochrome b to regulate mitochondrial translation. J Cell Biol. 2014;205:511-24 pubmed publisher
    ..Failure to hemylate cytochrome b sequesters the Cbp3-Cbp6 complex in early assembly intermediates, thereby causing a reduction in cytochrome b synthesis via a feedback loop that senses hemylation of cytochrome b. ..
  5. Levchenko M, Wuttke J, Römpler K, Schmidt B, Neifer K, Juris L, et al. Cox26 is a novel stoichiometric subunit of the yeast cytochrome c oxidase. Biochim Biophys Acta. 2016;1863:1624-32 pubmed publisher
    ..Our analyses reveal Cox26 as a novel stoichiometric structural subunit of the cytochrome c oxidase. A loss of Cox26 affects cytochrome c oxidase activity and respirasome organization. ..
  6. Stutz F, Kantor J, Zhang D, McCarthy T, Neville M, Rosbash M. The yeast nucleoporin rip1p contributes to multiple export pathways with no essential role for its FG-repeat region. Genes Dev. 1997;11:2857-68 pubmed
    The FG-repeat domain of the yeast Rip1 protein (Rip1p) was identified initially as a possible target for the nuclear export signal (NES) of the HIV-1 Rev protein in a yeast two-hybrid assay...
  7. Mick D, Vukotic M, Piechura H, Meyer H, Warscheid B, Deckers M, et al. Coa3 and Cox14 are essential for negative feedback regulation of COX1 translation in mitochondria. J Cell Biol. 2010;191:141-54 pubmed publisher
    ..Our data indicate that Coa1 binding to sequestered Mss51 in complex with Cox14, Coa3, and Cox1 is essential for full inactivation. ..
  8. Beckmann J, Ljungdahl P, Lopez J, Trumpower B. Isolation and characterization of the nuclear gene encoding the Rieske iron-sulfur protein (RIP1) from Saccharomyces cerevisiae. J Biol Chem. 1987;262:8901-9 pubmed
    ..Amino acid sequence analysis of the amino-terminal end of the mature iron-sulfur protein agreed exactly with that predicted by the nucleotide sequence starting at Lys-31.(ABSTRACT TRUNCATED AT 400 WORDS) ..
  9. Conte A, Papa B, Ferramosca A, Zara V. The dimerization of the yeast cytochrome bc1 complex is an early event and is independent of Rip1. Biochim Biophys Acta. 2015;1853:987-95 pubmed publisher
    ..Among them, the Rieske iron-sulfur protein (Rip1), besides its catalytic role in electron transfer, may be implicated in the bc1 complex dimerization...

More Information


  1. Deckers M, Balleininger M, Vukotic M, Römpler K, Bareth B, Juris L, et al. Aim24 stabilizes respiratory chain supercomplexes and is required for efficient respiration. FEBS Lett. 2014;588:2985-92 pubmed publisher
    ..Deletion of AIM24 strongly affects activity of the respiratory chain and induces a growth defect on non-fermentable medium. Our data indicate that Aim24 has a function in stabilizing the respiratory chain supercomplexes. ..
  2. Chen Y, Taylor E, Dephoure N, Heo J, Tonhato A, Papandreou I, et al. Identification of a protein mediating respiratory supercomplex stability. Cell Metab. 2012;15:348-60 pubmed publisher
    ..We suggest that Rcf1 is a member of an evolutionarily conserved protein family that acts to promote respiratory supercomplex assembly and activity. ..
  3. Strogolova V, Furness A, Robb McGrath M, Garlich J, Stuart R. Rcf1 and Rcf2, members of the hypoxia-induced gene 1 protein family, are critical components of the mitochondrial cytochrome bc1-cytochrome c oxidase supercomplex. Mol Cell Biol. 2012;32:1363-73 pubmed publisher
    ..Based on our findings, we propose a model where the Hig1 proteins regulate the COX enzyme activity through Cox3 and associated Cox12 protein, in a manner that may be influenced by the neighboring AAC proteins. ..
  4. Wagener N, Ackermann M, Funes S, Neupert W. A pathway of protein translocation in mitochondria mediated by the AAA-ATPase Bcs1. Mol Cell. 2011;44:191-202 pubmed publisher
    ..Bcs1 mediates topogenesis of the Rieske protein, Rip1, a component of respiratory chains in bacteria, mitochondria, and chloroplasts...
  5. Atkinson A, Smith P, Fox J, Cui T, Khalimonchuk O, Winge D. The LYR protein Mzm1 functions in the insertion of the Rieske Fe/S protein in yeast mitochondria. Mol Cell Biol. 2011;31:3988-96 pubmed publisher
    ..Formation of bc(1) is stalled in mzm1? cells at a late assembly intermediate lacking the Rieske iron-sulfur protein Rip1. Rip1 levels are markedly attenuated in mzm1? cells at elevated temperatures...
  6. Brandina I, Graham J, Lemaitre Guillier C, Entelis N, Krasheninnikov I, Sweetlove L, et al. Enolase takes part in a macromolecular complex associated to mitochondria in yeast. Biochim Biophys Acta. 2006;1757:1217-28 pubmed
    ..This suggests an unsuspected novel function for this complex in tRNA mitochondrial import. ..
  7. Lee J, Hwang G, Naganuma A. Rip1 enhances methylmercury toxicity through production of reactive oxygen species (ROS) in budding yeast. J Toxicol Sci. 2009;34:715-7 pubmed
    ..Resistance to methylmercury was exhibited only by yeast that lacked Rip1, a component of electron transport system complex III...
  8. Kronekova Z, Rödel G. Organization of assembly factors Cbp3p and Cbp4p and their effect on bc(1) complex assembly in Saccharomyces cerevisiae. Curr Genet. 2005;47:203-12 pubmed
  9. Cui T, Smith P, Fox J, Khalimonchuk O, Winge D. Late-stage maturation of the Rieske Fe/S protein: Mzm1 stabilizes Rip1 but does not facilitate its translocation by the AAA ATPase Bcs1. Mol Cell Biol. 2012;32:4400-9 pubmed publisher
    ..of the ubiquinol-cytochrome c reductase or bc(1) complex involves the insertion of the Rieske Fe/S cluster protein, Rip1. Maturation of Rip1 occurs within the mitochondrial matrix prior to its translocation across the inner membrane (IM)..
  10. Estruch F, Hodge C, Rodriguez Navarro S, Cole C. Physical and genetic interactions link the yeast protein Zds1p with mRNA nuclear export. J Biol Chem. 2005;280:9691-7 pubmed
    ..We proposed that Zds1p associates with the complex formed by Dbp5p, Gfd1p, and nucleoporins at the cytosolic fibrils of the nuclear pore complex and is required for optimal mRNA export. ..
  11. Murphy R, Watkins J, Wente S. GLE2, a Saccharomyces cerevisiae homologue of the Schizosaccharomyces pombe export factor RAE1, is required for nuclear pore complex structure and function. Mol Biol Cell. 1996;7:1921-37 pubmed
    ..We propose that Gle2p has a novel role in mediating nuclear transport. ..
  12. Murphy R, Wente S. An RNA-export mediator with an essential nuclear export signal. Nature. 1996;383:357-60 pubmed
    ..pore complex and requires a nuclear export signal (NES) and interaction with a cellular cofactor, the protein Rip1. Endogenous RNA export mediators that interact with Rip1 and harbour NESs are thought to exist but have yet to be ..
  13. Bareth B, Nikolov M, Lorenzi I, Hildenbeutel M, Mick D, Helbig C, et al. Oms1 associates with cytochrome c oxidase assembly intermediates to stabilize newly synthesized Cox1. Mol Biol Cell. 2016;27:1570-80 pubmed publisher
    ..Our analyses show that Oms1 participates in cytochrome c oxidase assembly by stabilizing newly synthesized Cox1. ..
  14. Römpler K, Müller T, Juris L, Wissel M, Vukotic M, Hofmann K, et al. Overlapping Role of Respiratory Supercomplex Factor Rcf2 and Its N-terminal Homolog Rcf3 in Saccharomyces cerevisiae. J Biol Chem. 2016;291:23769-23778 pubmed
    ..Furthermore, our data suggest an association of all three Rcf proteins with the bc1 complex in the absence of a functional cytochrome c oxidase and identify a supercomplex independent interaction network of the Rcf proteins. ..
  15. Gebert M, Schrempp S, Mehnert C, Heißwolf A, Oeljeklaus S, Ieva R, et al. Mgr2 promotes coupling of the mitochondrial presequence translocase to partner complexes. J Cell Biol. 2012;197:595-604 pubmed publisher
    ..We conclude that Mgr2 is a coupling factor of the presequence translocase crucial for cell growth at elevated temperature and for efficient protein import. ..
  16. Cui T, Conte A, Fox J, Zara V, Winge D. Modulation of the respiratory supercomplexes in yeast: enhanced formation of cytochrome oxidase increases the stability and abundance of respiratory supercomplexes. J Biol Chem. 2014;289:6133-41 pubmed publisher
    Yeast cells deficient in the Rieske iron-sulfur subunit (Rip1) of ubiquinol-cytochrome c reductase (bc1) accumulate a late core assembly intermediate, which weakly associates with cytochrome oxidase (CcO) in a respiratory supercomplex...
  17. Ljungdahl P, Beckmann J, Trumpower B. Mutational analysis of the mitochondrial Rieske iron-sulfur protein of Saccharomyces cerevisiae. II. Biochemical characterization of temperature-sensitive RIP1- mutations. J Biol Chem. 1989;264:3723-31 pubmed
    ..D., Ljungdahl, P. O., and Trumpower, B. L. (1989) J. Biol. Chem. 264, 3713-3722). Each of the five ts-rip1- mutants exhibited pleiotropic effects...