RHO1

Summary

Gene Symbol: RHO1
Description: Rho family GTPase RHO1
Alias: Rho family GTPase RHO1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Fitch P, Gammie A, Lee D, de Candal V, Rose M. Lrg1p Is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast. Genetics. 2004;168:733-46 pubmed
    ..We conclude that Lrg1p is a Rho1p-GAP involved in cell fusion and speculate that it acts to locally inhibit cell wall synthesis to aid in the close apposition of the plasma membranes of mating cells. ..
  2. Madden K, Snyder M. Cell polarity and morphogenesis in budding yeast. Annu Rev Microbiol. 1998;52:687-744 pubmed
    ..Since many of the components important for polarized cell growth are conserved in other organisms, the basic mechanisms mediating polarized cell growth are likely to be universal among eukaryotes. ..
  3. deHart A, Schnell J, Allen D, Tsai J, Hicke L. Receptor internalization in yeast requires the Tor2-Rho1 signaling pathway. Mol Biol Cell. 2003;14:4676-84 pubmed
    ..Yeast Tor2 has an additional role in regulating the integrity of the cell wall by activating the Rho1 guanine nucleotide exchange factor Rom2...
  4. Mack D, Nishimura K, Dennehey B, Arbogast T, Parkinson J, Toh e A, et al. Identification of the bud emergence gene BEM4 and its interactions with rho-type GTPases in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4387-95 pubmed
    ..Models for the role of Bem4p include that it serves as a chaperone or modulates the interaction of these GTPases with one or more of their targets or regulators. ..
  5. Lorberg A, Schmitz H, Jacoby J, Heinisch J. Lrg1p functions as a putative GTPase-activating protein in the Pkc1p-mediated cell integrity pathway in Saccharomyces cerevisiae. Mol Genet Genomics. 2001;266:514-26 pubmed
    ..We therefore suggest that Lrg1p acts as a negative regulator of the Pkc1p pathway in conjunction with its known homologue Sac7p. ..
  6. Yoshida S, Bartolini S, Pellman D. Mechanisms for concentrating Rho1 during cytokinesis. Genes Dev. 2009;23:810-23 pubmed publisher
    The small GTP-binding protein, Rho1/RhoA plays a central role in cytokinetic actomyosin ring (CAR) assembly and cytokinesis...
  7. Verna J, Lodder A, Lee K, Vagts A, Ballester R. A family of genes required for maintenance of cell wall integrity and for the stress response in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1997;94:13804-9 pubmed
    ..The functional characteristics and cellular localization of WSC suggest that they may mediate intracellular responses to environmental stress in yeast. ..
  8. Philip B, Levin D. Wsc1 and Mid2 are cell surface sensors for cell wall integrity signaling that act through Rom2, a guanine nucleotide exchange factor for Rho1. Mol Cell Biol. 2001;21:271-80 pubmed
    ..are required for activation of the cell wall integrity signaling pathway that consists of the small G-protein Rho1, protein kinase C (Pkc1), and a mitogen-activated protein kinase cascade...
  9. Guo W, Tamanoi F, Novick P. Spatial regulation of the exocyst complex by Rho1 GTPase. Nat Cell Biol. 2001;3:353-60 pubmed
    ..Here we report that certain rho1 mutant alleles specifically affect the localization of the exocyst proteins...

More Information

Publications76

  1. Kamada Y, Qadota H, Python C, Anraku Y, Ohya Y, Levin D. Activation of yeast protein kinase C by Rho1 GTPase. J Biol Chem. 1996;271:9193-6 pubmed
    We have investigated the role of the essential Rho1 GTPase in cell integrity signaling in budding yeast...
  2. Qadota H, Python C, Inoue S, Arisawa M, Anraku Y, Zheng Y, et al. Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science. 1996;272:279-81 pubmed
    ..Both proteins were localized predominantly at sites of cell wall remodeling. Therefore, it appears that Rho1p is a regulatory subunit of beta(1-->3)glucan synthase. ..
  3. Eitzen G, Thorngren N, Wickner W. Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking. EMBO J. 2001;20:5650-6 pubmed
    ..Thus, multiple GTPases act in a defined sequence to regulate the docking steps of vacuole fusion. ..
  4. Zhang X, Bi E, Novick P, Du L, Kozminski K, Lipschutz J, et al. Cdc42 interacts with the exocyst and regulates polarized secretion. J Biol Chem. 2001;276:46745-50 pubmed
    ..These results have revealed a role for Cdc42 in exocytosis. We propose that Cdc42 coordinates the vesicle docking machinery and the actin cytoskeleton for polarized secretion. ..
  5. Watanabe D, Abe M, Ohya Y. Yeast Lrg1p acts as a specialized RhoGAP regulating 1,3-beta-glucan synthesis. Yeast. 2001;18:943-51 pubmed
    ..in the LRG1 gene restore impaired 1,3-beta-glucan synthesis in the fks1-1154 Deltafks2 mutant as well as that in rho1-2, a temperature-sensitive mutant of Rho-type GTPase that functions as a regulatory subunit of GS...
  6. Sekiya Kawasaki M, Abe M, Saka A, Watanabe D, Kono K, Minemura Asakawa M, et al. Dissection of upstream regulatory components of the Rho1p effector, 1,3-beta-glucan synthase, in Saccharomyces cerevisiae. Genetics. 2002;162:663-76 pubmed
    ..Yeast GS is composed of a putative catalytic subunit encoded by FKS1 and FKS2 and a regulatory subunit encoded by RHO1. A combination of amino acid alterations in the putative catalytic domain of Fks1p was found to result in a loss of ..
  7. Yoshida S, Kono K, Lowery D, Bartolini S, Yaffe M, Ohya Y, et al. Polo-like kinase Cdc5 controls the local activation of Rho1 to promote cytokinesis. Science. 2006;313:108-11 pubmed
    ..We found that budding yeast Polo-like kinase Cdc5 controls the targeting and activation of Rho1 (RhoA) at the division site via Rho1 guanine nucleotide exchange factors...
  8. Andrews P, Stark M. Dynamic, Rho1p-dependent localization of Pkc1p to sites of polarized growth. J Cell Sci. 2000;113 ( Pt 15):2685-93 pubmed
    ..These results suggest that the roles of Pkc1p in both polarized growth and the response to cell wall stress are mediated by dynamic changes in its localization, and suggest an additional potential role in cytokinesis. ..
  9. Kono K, Nogami S, Abe M, Nishizawa M, Morishita S, Pellman D, et al. G1/S cyclin-dependent kinase regulates small GTPase Rho1p through phosphorylation of RhoGEF Tus1p in Saccharomyces cerevisiae. Mol Biol Cell. 2008;19:1763-71 pubmed publisher
    ..We propose that Cln2p/Cdc28p-dependent phosphorylation of Tus1p is required for appropriate temporal and spatial activation of Rho1p at the G1/S transition. ..
  10. McCaffrey M, Johnson J, Goud B, Myers A, Rossier J, Popoff M, et al. The small GTP-binding protein Rho1p is localized on the Golgi apparatus and post-Golgi vesicles in Saccharomyces cerevisiae. J Cell Biol. 1991;115:309-19 pubmed
    ..Thus, in S. cerevisiae Rho1p resides primarily in the Golgi apparatus, and also in vesicles that are likely to be early post-Golgi vesicles. ..
  11. Mazur P, Baginsky W. In vitro activity of 1,3-beta-D-glucan synthase requires the GTP-binding protein Rho1. J Biol Chem. 1996;271:14604-9 pubmed
    ..The regulation of 1,3-beta-D-glucan synthase activity by RHO1 is consistent with its observed role in morphogenetic control and osmotic integrity.
  12. Kohno H, Tanaka K, Mino A, Umikawa M, Imamura H, Fujiwara T, et al. Bni1p implicated in cytoskeletal control is a putative target of Rho1p small GTP binding protein in Saccharomyces cerevisiae. EMBO J. 1996;15:6060-8 pubmed
    The RHO1 gene encodes a homolog of mammalian RhoA small GTP binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth sites, including the bud tip and the cytokinesis site, and is required for bud formation...
  13. Fujiwara T, Tanaka K, Mino A, Kikyo M, Takahashi K, Shimizu K, et al. Rho1p-Bni1p-Spa2p interactions: implication in localization of Bni1p at the bud site and regulation of the actin cytoskeleton in Saccharomyces cerevisiae. Mol Biol Cell. 1998;9:1221-33 pubmed
    ..These results indicate that both Rho1p and Spa2p are involved in the localization of Bni1p at the growth sites where Rho1p regulates reorganization of the actin cytoskeleton through Bni1p. ..
  14. Gao X, Caviston J, Tcheperegine S, Bi E. Pxl1p, a paxillin-like protein in Saccharomyces cerevisiae, may coordinate Cdc42p and Rho1p functions during polarized growth. Mol Biol Cell. 2004;15:3977-85 pubmed
    ..However, Pxl1p directly binds to Rho1p-GDP in vitro, and inhibits the growth of rho1-2 and rho1-3 mutants in a dosage-dependent manner...
  15. Yamochi W, Tanaka K, Nonaka H, Maeda A, Musha T, Takai Y. Growth site localization of Rho1 small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae. J Cell Biol. 1994;125:1077-93 pubmed
    ..In the yeast Saccharomyces cerevisiae, there is a homologue of mammalian RhoA, RHO1, which is essential for vegetative growth of yeast cells...
  16. Drgonova J, Drgon T, Tanaka K, Kollar R, Chen G, Ford R, et al. Rho1p, a yeast protein at the interface between cell polarization and morphogenesis. Science. 1996;272:277-9 pubmed
    ..The GTP binding protein was identified as Rho1p. The rho1 mutants were defective in GTP stimulation of glucan synthase, and the defect was corrected by addition of purified ..
  17. Ozaki K, Tanaka K, Imamura H, Hihara T, Kameyama T, Nonaka H, et al. Rom1p and Rom2p are GDP/GTP exchange proteins (GEPs) for the Rho1p small GTP binding protein in Saccharomyces cerevisiae. EMBO J. 1996;15:2196-207 pubmed
    The RHO1 gene encodes a homolog of the mammalian RhoA small GTP binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth site and is required for bud formation...
  18. Kono K, Saeki Y, Yoshida S, Tanaka K, Pellman D. Proteasomal degradation resolves competition between cell polarization and cellular wound healing. Cell. 2012;150:151-64 pubmed publisher
    ..Mechanisms to overcome competition from a pre-existing polarized cytoskeleton may be a general feature of effective wound healing in polarized cells. ..
  19. Nonaka H, Tanaka K, Hirano H, Fujiwara T, Kohno H, Umikawa M, et al. A downstream target of RHO1 small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae. EMBO J. 1995;14:5931-8 pubmed
    The RHO1 gene in Saccharomyces cerevisiae encodes a homolog of the mammalian RhoA small GTP-binding protein, which is implicated in various actin cytoskeleton-dependent cell functions. In yeast, Rho1p is involved in bud formation...
  20. Ketela T, Green R, Bussey H. Saccharomyces cerevisiae mid2p is a potential cell wall stress sensor and upstream activator of the PKC1-MPK1 cell integrity pathway. J Bacteriol. 1999;181:3330-40 pubmed
    ..mutants can be suppressed by overexpression of upstream elements of the cell integrity pathway, including PKC1, RHO1, WSC1, and WSC2...
  21. Liao Y, He F, Gong T, Bi E, Gao X. Msb1 interacts with Cdc42, Boi1, and Boi2 and may coordinate Cdc42 and Rho1 functions during early stage of bud development in budding yeast. PLoS ONE. 2013;8:e66321 pubmed publisher
    ..We also show that Msb1 interacts with Rho1 in the cells and Msb1 overproduction inhibits the growth of rho1-104 and rho1-3 but not rho1-2 cells...
  22. Liou L, Ren Q, Gao Q, Zhang Z. Sro7 and Sro77, the yeast homologues of the Drosophila lethal giant larvae (Lgl), regulate cell proliferation via the Rho1-Tor1 pathway. Microbiology. 2014;160:2208-14 pubmed publisher
    ..Overexpression of RHO1, one of the central regulators of cell polarity and CWI, fully recovered the sro7?/sro77? phenotype...
  23. Kim Y, Francisco L, Chen G, Marcotte E, Chan C. Control of cellular morphogenesis by the Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J Cell Biol. 1994;127:1381-94 pubmed
    ..The Rho1 and Rho2 GTPases are the likely in vivo targets of Bem2p because bem2 mutant phenotypes can be partially suppressed ..
  24. Cardon C, Beck T, Hall M, Rutter J. PAS kinase promotes cell survival and growth through activation of Rho1. Sci Signal. 2012;5:ra9 pubmed publisher
    ..phosphorylation of the metabolic enzyme Ugp1 on serine 11, this resulted in the formation of a complex that induced Rho1 activation, rather than required the glucose partitioning function of Ugp1...
  25. Tcheperegine S, Gao X, Bi E. Regulation of cell polarity by interactions of Msb3 and Msb4 with Cdc42 and polarisome components. Mol Cell Biol. 2005;25:8567-80 pubmed
    ..We also show that Msb3 and Msb4 bind specifically to Cdc42-GDP and Rho1-GDP in vitro and that Msb3 and Rho GDP dissociation inhibitor act independently but oppositely on Cdc42...
  26. Ohya Y, Caplin B, Qadota H, Tibbetts M, Anraku Y, Pringle J, et al. Mutational analysis of the beta-subunit of yeast geranylgeranyl transferase I. Mol Gen Genet. 1996;252:1-10 pubmed
    ..Thus, several phenotypic differences are observed among the cal1/cdc43 mutations, possibly due to alterations in substrate specificity caused by the mutations. ..
  27. Logan M, Jones L, Forsberg D, Bodman A, Baier A, Eitzen G. Functional analysis of RhoGDI inhibitory activity on vacuole membrane fusion. Biochem J. 2011;434:445-57 pubmed publisher
    ..Furthermore, Rdi1p inhibited membrane fusion through the binding of Rho GTPases independent from its extraction activity. ..
  28. Fadri M, Daquinag A, Wang S, Xue T, Kunz J. The pleckstrin homology domain proteins Slm1 and Slm2 are required for actin cytoskeleton organization in yeast and bind phosphatidylinositol-4,5-bisphosphate and TORC2. Mol Biol Cell. 2005;16:1883-900 pubmed
  29. Li X, Ohmori T, Irie K, Kimura Y, Suda Y, Mizuno T, et al. Different Regulations of ROM2 and LRG1 Expression by Ccr4, Pop2, and Dhh1 in the Saccharomyces cerevisiae Cell Wall Integrity Pathway. mSphere. 2016;1: pubmed
    ..Ccr4 and Khd1 modulate a signal from Rho1 small GTPase in the CWI pathway by regulating the expression of ROM2 mRNA and LRG1 mRNA, encoding a ..
  30. Hatakeyama R, Kono K, Yoshida S. Ypk1 and Ypk2 kinases maintain Rho1 at the plasma membrane by flippase-dependent lipid remodeling after membrane stresses. J Cell Sci. 2017;130:1169-1178 pubmed publisher
    ..kinase cascade plays a crucial role in PM stress responses by reorganizing the actin cytoskeleton via Rho1 GTPase. However, the molecular mechanism by which TORC2-Ypk1/Ypk2 regulates Rho1 is not well defined...
  31. Marelli M, Smith J, Jung S, Yi E, Nesvizhskii A, Christmas R, et al. Quantitative mass spectrometry reveals a role for the GTPase Rho1p in actin organization on the peroxisome membrane. J Cell Biol. 2004;167:1099-112 pubmed
    ..Rho1p regulates the assembly state of actin on the peroxisome membrane, thereby controlling peroxisome membrane dynamics and biogenesis. ..
  32. Xu H, Wickner W. Bem1p is a positive regulator of the homotypic fusion of yeast vacuoles. J Biol Chem. 2006;281:27158-66 pubmed
    ..2005) Genes Dev. 19, 2606-2618), we did not find phosphorylation of Bem1p at Ser-72 to be required for Bem1p-stimulated fusion. Taken together, Bem1p is a positive regulator of lipid mixing during vacuole hemifusion and fusion. ..
  33. Schmitz H, Lorberg A, Heinisch J. Regulation of yeast protein kinase C activity by interaction with the small GTPase Rho1p through its amino-terminal HR1 domain. Mol Microbiol. 2002;44:829-40 pubmed
    ..The intragenic lethality of mutants affected in both HR1A and the C1 domain reported in this work implies an essential role for Rho1p-Pkc1p interaction in yeast. ..
  34. Roumanie O, Weinachter C, Larrieu I, Crouzet M, Doignon F. Functional characterization of the Bag7, Lrg1 and Rgd2 RhoGAP proteins from Saccharomyces cerevisiae. FEBS Lett. 2001;506:149-56 pubmed
    ..and then using an in vitro assay, we have demonstrated that the Bag7 protein stimulated the GTPase activity of the Rho1 protein, Lrg1p acted on the Cdc42 and Rho2 GTPases and we showed that Rgd2p has a GAP activity on both Cdc42p and ..
  35. Martin Yken H, Dagkessamanskaia A, de Groot P, Ram A, Klis F, Francois J. Saccharomyces cerevisiae YCRO17c/CWH43 encodes a putative sensor/transporter protein upstream of the BCK2 branch of the PKC1-dependent cell wall integrity pathway. Yeast. 2001;18:827-40 pubmed
  36. Guo S, Shen X, Yan G, Ma D, Bai X, Li S, et al. A MAP kinase dependent feedback mechanism controls Rho1 GTPase and actin distribution in yeast. PLoS ONE. 2009;4:e6089 pubmed publisher
    In the yeast Saccharomyces cerevisiae the guanosine triphosphatase (GTPase) Rho1 controls actin polarization and cell wall expansion...
  37. Koch G, Tanaka K, Masuda T, Yamochi W, Nonaka H, Takai Y. Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor (Rho GDI) in Saccharomyces cerevisiae. Oncogene. 1997;15:417-22 pubmed
    ..These results indicate that yeast Rho GDI possesses properties similar to those of mammalian Rho GDI, and that there is a cytosolic factor which functionally substitutes for Rho GDI in yeast. ..
  38. Schmidt A, Bickle M, Beck T, Hall M. The yeast phosphatidylinositol kinase homolog TOR2 activates RHO1 and RHO2 via the exchange factor ROM2. Cell. 1997;88:531-42 pubmed
    ..Overexpression of RHO1 or RHO2, encoding Rho-like GTPases, or ROM2, encoding a GDP/GTP exchange factor for RHO1 and RHO2, suppresses a ..
  39. Kikuchi Y, Mizuuchi E, Nogami S, Morishita S, Ohya Y. Involvement of Rho-type GTPase in control of cell size in Saccharomyces cerevisiae. FEMS Yeast Res. 2007;7:569-78 pubmed
    ..One group of rho1 mutants (rho1A) underwent premature entry into mitosis, leading to the birth of abnormally small cells...
  40. Yamashita M, Kurokawa K, Sato Y, Yamagata A, Mimura H, Yoshikawa A, et al. Structural basis for the Rho- and phosphoinositide-dependent localization of the exocyst subunit Sec3. Nat Struct Mol Biol. 2010;17:180-6 pubmed publisher
    ..yeast exocyst subunit Sec3 binds to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) and the small GTPases Rho1 and Cdc42 via its N-terminal domain (Sec3-N), and these interactions target Sec3 to the plasma membrane...
  41. Yan G, Lai Y, Jiang Y. TOR under stress: targeting TORC1 by Rho1 GTPase. Cell Cycle. 2012;11:3384-8 pubmed publisher
    In the yeast Saccharomyces cerevisiae, small GTPase Rho1 controls polarized actin distribution and cell wall expansion in response to many different environmental and intracellular stimuli...
  42. Bourbonnais Y, Faucher N, Pallotta D, Larouche C. Multiple cellular processes affected by the absence of the Rpb4 subunit of RNA polymerase II contribute to the deficiency in the stress response of the yeast rpb4(delta) mutant. Mol Gen Genet. 2001;264:763-72 pubmed
    ..An increase dosage of ZDS1, or of genes involved in cell wall assembly and in secretion (RHO1 and SR077, respectively), partially suppresses the sensitivity of rpb4delta cells to high temperature, heat shock ..
  43. Boscheron C, Caudron F, Loeillet S, Peloso C, Mugnier M, Kurzawa L, et al. A role for the yeast CLIP170 ortholog, the plus-end-tracking protein Bik1, and the Rho1 GTPase in Snc1 trafficking. J Cell Sci. 2016;129:3332-41 pubmed publisher
    ..Finally, constitutively active Rho1 rescued both Bik1 localization at the microtubule plus-ends in tub1-Glu strain and a correct Snc1 trafficking in a ..
  44. Mizunuma M, Hirata D, Miyakawa T. Implication of Pkc1p protein kinase C in sustaining Cln2p level and polarized bud growth in response to calcium signaling in Saccharomyces cerevisiae. J Cell Sci. 2005;118:4219-29 pubmed
    ..This phenotype was caused by a decreased expression level of the G1 cyclin Cln2p. The Rho1 small G protein molecular switch was suggested to be involved in the novel Pkc1p function...
  45. Xu S, Zhang G, Zhang H, Kitajima T, Nakanishi H, Gao X. Effects of Rho1, a small GTPase on the production of recombinant glycoproteins in Saccharomyces cerevisiae. Microb Cell Fact. 2016;15:179 pubmed
    ..In this study, overexpression of RHO1, which encodes the Rho1p small GTPase, is confirmed to partially recover the growth defect of Saccharomyces ..
  46. Schmidt A, Schmelzle T, Hall M. The RHO1-GAPs SAC7, BEM2 and BAG7 control distinct RHO1 functions in Saccharomyces cerevisiae. Mol Microbiol. 2002;45:1433-41 pubmed
    In Saccharomyces cerevisiae, the small GTPase RHO1 plays an essential role in the control of cell wall synthesis and organization of the actin cytoskeleton...
  47. Nomura W, Inoue Y. Contribution of phosphatidylserine to Rho1- and Pkc1-related repolarization of the actin cytoskeleton under stressed conditions in Saccharomyces cerevisiae. Small Gtpases. 2017;:1-7 pubmed publisher
    ..b>Rho1, one of the Rho-type GTPases in S...
  48. Alfaro G, Johansen J, Dighe S, Duamel G, Kozminski K, Beh C. The sterol-binding protein Kes1/Osh4p is a regulator of polarized exocytosis. Traffic. 2011;12:1521-36 pubmed publisher
    ..These results support a model in which Osh4p acts as a sterol-dependent regulator of polarized vesicle transport, as opposed to being a sterol-transfer protein. ..
  49. Prosser D, Pannunzio A, Brodsky J, Thorner J, Wendland B, O Donnell A. α-Arrestins participate in cargo selection for both clathrin-independent and clathrin-mediated endocytosis. J Cell Sci. 2015;128:4220-34 pubmed publisher
    ..cerevisiae, a widely used model for studying CME, was recently shown to have a CIE pathway that requires the GTPase Rho1, the formin Bni1, and their regulators...
  50. Meitinger F, Richter H, Heisel S, Hub B, Seufert W, Pereira G. A safeguard mechanism regulates Rho GTPases to coordinate cytokinesis with the establishment of cell polarity. PLoS Biol. 2013;11:e1001495 pubmed publisher
    ..protein Gps1 (GTPase-mediated polarity switch 1) establishes a novel polarity cue that concomitantly sustains Rho1-dependent polarization and inhibits premature Cdc42 activation at the site of cytokinesis...
  51. Bar E, Ellicott A, Stone D. Gbetagamma recruits Rho1 to the site of polarized growth during mating in budding yeast. J Biol Chem. 2003;278:21798-804 pubmed
    ..We identified RHO1 in a screen for allele-specific high-copy suppressors of Gbetagamma overexpression, suggesting that Rho1 binds ..
  52. Queralt E, Igual J. Functional connection between the Clb5 cyclin, the protein kinase C pathway and the Swi4 transcription factor in Saccharomyces cerevisiae. Genetics. 2005;171:1485-98 pubmed
    ..In summary, we report numerous genetic interactions and phenotypic descriptions supporting a close functional relationship between the Clb5 cyclin, the PKC pathway, and the Swi4 transcription factor. ..
  53. Alberts A, Bouquin N, Johnston L, Treisman R. Analysis of RhoA-binding proteins reveals an interaction domain conserved in heterotrimeric G protein beta subunits and the yeast response regulator protein Skn7. J Biol Chem. 1998;273:8616-22 pubmed
    ..We show that beta2 and Skn7 can interact with mammalian RhoA and Cdc42 and yeast Rho1, both in vivo and in vitro...
  54. Logan M, Jones L, Eitzen G. Cdc42p and Rho1p are sequentially activated and mechanistically linked to vacuole membrane fusion. Biochem Biophys Res Commun. 2010;394:64-9 pubmed publisher
    ..These results define unique activation mechanisms for Cdc42p and Rho1p, which may be linked to the vacuole membrane fusion mechanism. ..
  55. Ho H, Lee H, Liao H, Chen M. Involvement of Saccharomyces cerevisiae Avo3p/Tsc11p in maintaining TOR complex 2 integrity and coupling to downstream signaling. Eukaryot Cell. 2008;7:1328-43 pubmed publisher
    ..Our results also suggest that Avo2p/Slm1p-mediated signaling, but not Avo1p-mediated signaling, links to Rho1p activation specifically through the Rho1p-guanine nucleotide exchange factor Tus1p. ..
  56. Onishi M, Ko N, Nishihama R, Pringle J. Distinct roles of Rho1, Cdc42, and Cyk3 in septum formation and abscission during yeast cytokinesis. J Cell Biol. 2013;202:311-29 pubmed publisher
    In yeast and animal cytokinesis, the small guanosine triphosphatase (GTPase) Rho1/RhoA has an established role in formation of the contractile actomyosin ring, but its role, if any, during cleavage-furrow ingression and abscission is ..
  57. Helliwell S, Schmidt A, Ohya Y, Hall M. The Rho1 effector Pkc1, but not Bni1, mediates signalling from Tor2 to the actin cytoskeleton. Curr Biol. 1998;8:1211-4 pubmed
    ..Tor2 controls the cell-cycle-dependent organisation of the actin cytoskeleton by activating the small GTPase Rho1 via the exchange factor Rom2 [1,2]...
  58. Juanes M, Queralt E, Bañó M, Igual J. Rot1 plays an antagonistic role to Clb2 in actin cytoskeleton dynamics throughout the cell cycle. J Cell Sci. 2007;120:2390-401 pubmed
    ..Several genetic interactions suggest a link between Rot1 and the ubiquitin-proteasome system and we show that the Clb2 cyclin is not properly degraded in rot1 cells. ..
  59. de Bettignies G, Thoraval D, Morel C, Peypouquet M, Crouzet M. Overactivation of the protein kinase C-signaling pathway suppresses the defects of cells lacking the Rho3/Rho4-GAP Rgd1p in Saccharomyces cerevisiae. Genetics. 2001;159:1435-48 pubmed
    ..b>RHO1 and RHO2 encoding two small GTPases, MKK1 encoding one of the MAP-kinase kinases in the protein kinase C (PKC) ..
  60. Qadota H, Ishii I, Fujiyama A, Ohya Y, Anraku Y. RHO gene products, putative small GTP-binding proteins, are important for activation of the CAL1/CDC43 gene product, a protein geranylgeranyltransferase in Saccharomyces cerevisiae. Yeast. 1992;8:735-41 pubmed
    ..They are identical to the yeast RHO1 and RHO2 genes, which encode putative small GTP-binding proteins...
  61. Merlini L, Bolognesi A, Juanes M, Vandermoere F, Courtellemont T, Pascolutti R, et al. Rho1- and Pkc1-dependent phosphorylation of the F-BAR protein Syp1 contributes to septin ring assembly. Mol Biol Cell. 2015;26:3245-62 pubmed publisher
    ..Through a genetic screen, we uncovered an unanticipated link between the conserved Rho1 GTPase and its effector protein kinase C (Pkc1) with septin ring stability in yeast...
  62. Kozminski K, Alfaro G, Dighe S, Beh C. Homologues of oxysterol-binding proteins affect Cdc42p- and Rho1p-mediated cell polarization in Saccharomyces cerevisiae. Traffic. 2006;7:1224-42 pubmed
    ..Disruption of all OSH gene function caused specific defects in polarized exocytosis, indicating that the Osh proteins are collectively required for a secretory pathway implicated in the maintenance of polarized growth. ..
  63. Anastasia S, Nguyen D, Thai V, Meloy M, Macdonough T, Kellogg D. A link between mitotic entry and membrane growth suggests a novel model for cell size control. J Cell Biol. 2012;197:89-104 pubmed publisher
    ..Checkpoint signals are relayed by the Rho1 GTPase, protein kinase C (Pkc1), and a specific form of protein phosphatase 2A (PP2A(Cdc55))...
  64. Peterson J, Zheng Y, Bender L, Myers A, Cerione R, Bender A. Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast. J Cell Biol. 1994;127:1395-406 pubmed
    ..Also, these findings raise the possibility that Bem1p may regulate or be a target of action of one or more of these GTPases. ..
  65. Hirano H, Tanaka K, Ozaki K, Imamura H, Kohno H, Hihara T, et al. ROM7/BEM4 encodes a novel protein that interacts with the Rho1p small GTP-binding protein in Saccharomyces cerevisiae. Mol Cell Biol. 1996;16:4396-403 pubmed
    The RHO1 gene encodes a homolog of the mammalian RhoA small GTP-binding protein in the yeast Saccharomyces cerevisiae. Rho1p is localized at the growth site and is required for bud formation...
  66. Prosser D, Drivas T, Maldonado Baez L, Wendland B. Existence of a novel clathrin-independent endocytic pathway in yeast that depends on Rho1 and formin. J Cell Biol. 2011;195:657-71 pubmed publisher
    ..proteins Ent1, Ent2, Yap1801, and Yap1802, we identify a second endocytic pathway that depends on the GTPase Rho1, the downstream formin Bni1, and the Bni1 cofactors Bud6 and Spa2...
  67. Yan G, Lai Y, Jiang Y. The TOR complex 1 is a direct target of Rho1 GTPase. Mol Cell. 2012;45:743-53 pubmed publisher
    ..In this study, we show that stresses affect TORC1 function through Rho1, a member of Rho family GTPases...