Gene Symbol: RDH54
Description: DNA-dependent ATPase RDH54
Alias: TID1, DNA-dependent ATPase RDH54
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Shinohara M, Sakai K, Shinohara A, Bishop D. Crossover interference in Saccharomyces cerevisiae requires a TID1/RDH54- and DMC1-dependent pathway. Genetics. 2003;163:1273-86 pubmed
    ..Two partially redundant proteins, Rad54 and Tid1/Rdh54, act as recombinase accessory factors...
  2. Chi P, Kwon Y, Seong C, Epshtein A, Lam I, Sung P, et al. Yeast recombination factor Rdh54 functionally interacts with the Rad51 recombinase and catalyzes Rad51 removal from DNA. J Biol Chem. 2006;281:26268-79 pubmed
    The Saccharomyces cerevisiae RDH54-encoded product, a member of the Swi2/Snf2 protein family, is needed for mitotic and meiotic interhomologue recombination and DNA repair...
  3. Dresser M, Ewing D, Conrad M, Dominguez A, Barstead R, Jiang H, et al. DMC1 functions in a Saccharomyces cerevisiae meiotic pathway that is largely independent of the RAD51 pathway. Genetics. 1997;147:533-44 pubmed
    ..Interestingly, the pattern on CHEF gels of chromosome fragments that result from meiotic DNA double-strand break formation is different in DMC1 mutant strains from that seen in rad50S strains. ..
  4. Petukhova G, Sung P, Klein H. Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1. Genes Dev. 2000;14:2206-15 pubmed
    The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 ..
  5. Signon L, Malkova A, Naylor M, Klein H, Haber J. Genetic requirements for RAD51- and RAD54-independent break-induced replication repair of a chromosomal double-strand break. Mol Cell Biol. 2001;21:2048-56 pubmed
    ..DSB-induced gene conversion is not significantly affected when RAD50, RAD59, TID1 (RDH54), SRS2, or SGS1 is deleted...
  6. Shah P, Zheng X, Epshtein A, Carey J, Bishop D, Klein H. Swi2/Snf2-related translocases prevent accumulation of toxic Rad51 complexes during mitotic growth. Mol Cell. 2010;39:862-72 pubmed publisher
    Purified DNA translocases Rdh54 and Rad54 can dissociate complexes formed by eukaryotic RecA-like recombinases on double-stranded DNA. Here, we show that Rad51 complexes are dissociated by these translocases in mitotic cells...
  7. Lee S, Pellicioli A, Malkova A, Foiani M, Haber J. The Saccharomyces recombination protein Tid1p is required for adaptation from G2/M arrest induced by a double-strand break. Curr Biol. 2001;11:1053-7 pubmed
    ..We suggest that Tid1p participates in monitoring the extent of single-stranded DNA produced by resection of DNA ends in a fashion that is distinct from its role in recombination. ..
  8. Holzen T, Shah P, Olivares H, Bishop D. Tid1/Rdh54 promotes dissociation of Dmc1 from nonrecombinogenic sites on meiotic chromatin. Genes Dev. 2006;20:2593-604 pubmed
    The meiosis-specific recombinase Dmc1 plays a critical role in DNA strand exchange in budding yeast. Tid1/Rdh54, a member of the Swi2/Snf2 family of DNA translocases, has been shown to stimulate Dmc1-dependent recombination...
  9. Shinohara M, Shita Yamaguchi E, Buerstedde J, Shinagawa H, Ogawa H, Shinohara A. Characterization of the roles of the Saccharomyces cerevisiae RAD54 gene and a homologue of RAD54, RDH54/TID1, in mitosis and meiosis. Genetics. 1997;147:1545-56 pubmed
    ..The yeast genome project revealed a homologue of RAD54, RDH54/TID1. Properties of the rdh54/tid1 mutant and the rad54 rdh54/tid1 double mutant are shown for mitosis and meiosis...

More Information


  1. Prasad T, Robertson R, Visnapuu M, Chi P, Sung P, Greene E. A DNA-translocating Snf2 molecular motor: Saccharomyces cerevisiae Rdh54 displays processive translocation and extrudes DNA loops. J Mol Biol. 2007;369:940-53 pubmed
    ..fluorescence microscopy (TIRFM) to investigate the characteristics of the yeast homologous recombination factor Rdh54 on DNA...
  2. Klein H. RDH54, a RAD54 homologue in Saccharomyces cerevisiae, is required for mitotic diploid-specific recombination and repair and for meiosis. Genetics. 1997;147:1533-43 pubmed
    ..The RDH54 gene is homologous to the RAD54 gene, but rdh54 mutants do not show sensitivity to methyl methanesulfonate at ..
  3. Nimonkar A, Amitani I, Baskin R, Kowalczykowski S. Single molecule imaging of Tid1/Rdh54, a Rad54 homolog that translocates on duplex DNA and can disrupt joint molecules. J Biol Chem. 2007;282:30776-84 pubmed
    The Saccharomyces cerevisiae Tid1 protein is important for the recombinational repair of double-stranded DNA breaks during meiosis. Tid1 is a member of Swi2/Snf2 family of chromatin remodeling proteins and shares homology with Rad54...
  4. Chi P, Kwon Y, Visnapuu M, Lam I, Santa Maria S, Zheng X, et al. Analyses of the yeast Rad51 recombinase A265V mutant reveal different in vivo roles of Swi2-like factors. Nucleic Acids Res. 2011;39:6511-22 pubmed publisher
    The Saccharomyces cerevisiae Swi2-like factors Rad54 and Rdh54 play multifaceted roles in homologous recombination via their DNA translocase activity...
  5. Nimonkar A, Dombrowski C, Siino J, Stasiak A, Stasiak A, Kowalczykowski S. Saccharomyces cerevisiae Dmc1 and Rad51 proteins preferentially function with Tid1 and Rad54 proteins, respectively, to promote DNA strand invasion during genetic recombination. J Biol Chem. 2012;287:28727-37 pubmed publisher
    The Saccharomyces cerevisiae Dmc1 and Tid1 proteins are required for the pairing of homologous chromosomes during meiotic recombination...
  6. Tsaponina O, Haber J. Frequent Interchromosomal Template Switches during Gene Conversion in S. cerevisiae. Mol Cell. 2014;55:615-25 pubmed publisher
    ..We identify the chromatin remodeler Rdh54 as the first protein required for template switching that does not affect simple gene conversion.
  7. Lisby M, Barlow J, Burgess R, Rothstein R. Choreography of the DNA damage response: spatiotemporal relationships among checkpoint and repair proteins. Cell. 2004;118:699-713 pubmed
  8. Xiong L, Chen X, Silver H, Ahmed N, Johnson E. Deficient SUMO attachment to Flp recombinase leads to homologous recombination-dependent hyperamplification of the yeast 2 microm circle plasmid. Mol Biol Cell. 2009;20:1241-51 pubmed publisher
    ..This work also illustrates the importance of using cir(o) strains when studying mutants that affect the yeast SUMO pathway, to avoid confusing direct functions of the SUMO pathway with secondary effects of 2 microm amplification. ..
  9. Kraus E, Leung W, Haber J. Break-induced replication: a review and an example in budding yeast. Proc Natl Acad Sci U S A. 2001;98:8255-62 pubmed
    ..Thus, the end of a linearized DNA fragment can initiate new DNA synthesis by BIR in which the newly synthesized DNA is displaced and subsequently forms circles by NHEJ. ..
  10. Kateneva A, Konovchenko A, Guacci V, Dresser M. Recombination protein Tid1p controls resolution of cohesin-dependent linkages in meiosis in Saccharomyces cerevisiae. J Cell Biol. 2005;171:241-53 pubmed
    ..We propose that Tid1p remodels Mcd1p-mediated cohesion early in meiotic prophase to facilitate interhomologue recombination and the subsequent segregation of homologous chromosomes. ..
  11. Shor E, Gangloff S, Wagner M, Weinstein J, Price G, Rothstein R. Mutations in homologous recombination genes rescue top3 slow growth in Saccharomyces cerevisiae. Genetics. 2002;162:647-62 pubmed
    ..In contrast, top3 mutants show extreme synergistic growth defects with mutations in RAD50, MRE11, XRS2, RDH54, and RAD1...
  12. Latypov V, Kozhina T, Kozhin S, Korolev V. [The Rdh54 protein role in regulation of DNA repair in yeast Saccharomyces cerevisiae]. Genetika. 2010;46:194-202 pubmed
    In this work, we present the evidences of the involvement of Rdh54 in coordination of DNA repair by several pathways...
  13. Ferrari M, Nachimuthu B, Donnianni R, Klein H, Pellicioli A. Tid1/Rdh54 translocase is phosphorylated through a Mec1- and Rad53-dependent manner in the presence of DSB lesions in budding yeast. DNA Repair (Amst). 2013;12:347-55 pubmed publisher
    ..The recombination factor Tid1/Rdh54, a member of the Swi2-like family proteins, has an ATPase activity and may contribute to the remodelling of ..
  14. Santa Maria S, Kwon Y, Sung P, Klein H. Characterization of the interaction between the Saccharomyces cerevisiae Rad51 recombinase and the DNA translocase Rdh54. J Biol Chem. 2013;288:21999-2005 pubmed publisher
    The Saccharomyces cerevisiae Rdh54 protein is a member of the Swi2/Snf2 family of DNA translocases required for meiotic and mitotic recombination and DNA repair...
  15. Kwon Y, Seong C, Chi P, Greene E, Klein H, Sung P. ATP-dependent chromatin remodeling by the Saccharomyces cerevisiae homologous recombination factor Rdh54. J Biol Chem. 2008;283:10445-52 pubmed publisher
    Saccharomyces cerevisiae RDH54 is a key member of the evolutionarily conserved RAD52 epistasis group of genes needed for homologous recombination and DNA double strand break repair...
  16. Haber J, Braberg H, Wu Q, Alexander R, Haase J, Ryan C, et al. Systematic triple-mutant analysis uncovers functional connectivity between pathways involved in chromosome regulation. Cell Rep. 2013;3:2168-78 pubmed publisher
    ..After subjecting asf1? cac1? to TMA, we found that the Swi/Snf Rdh54 protein compensates for the absence of Asf1 and Cac1...
  17. Busygina V, Gaines W, Xu Y, Kwon Y, Williams G, Lin S, et al. Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1. DNA Repair (Amst). 2013;12:707-12 pubmed publisher
    ..ScDmc1 so prepared is capable of forming D-loops and responsive to its accessory factors Rad54 and Rdh54. Negative staining electron microscopy and iterative helical real-space reconstruction revealed that the ScDmc1-..