Gene Symbol: RAS1
Description: Ras family GTPase RAS1
Alias: Ras family GTPase RAS1
Species: Saccharomyces cerevisiae S288c

Top Publications

  1. Ho J, Bretscher A. Ras regulates the polarity of the yeast actin cytoskeleton through the stress response pathway. Mol Biol Cell. 2001;12:1541-55 pubmed
    ..All the phenotypes of ras2Delta cells can be fully suppressed by expression of yeast RAS1 or RAS2 genes, human Ha-ras, or the double disruption of the stress response genes msn2Deltamsn4Delta...
  2. Yoshida S, Ichihashi R, Toh e A. Ras recruits mitotic exit regulator Lte1 to the bud cortex in budding yeast. J Cell Biol. 2003;161:889-97 pubmed
    ..These results suggest that Lte1 is a downstream effector protein of Ras in mitotic exit and that the Ras GEF domain of Lte1 is not essential for mitotic exit but required for its localization. ..
  3. Mösch H, Kübler E, Krappmann S, Fink G, Braus G. Crosstalk between the Ras2p-controlled mitogen-activated protein kinase and cAMP pathways during invasive growth of Saccharomyces cerevisiae. Mol Biol Cell. 1999;10:1325-35 pubmed
    ..Here we show that haploid invasive growth development depends on RAS2 but not RAS1. Ras1p is not sufficiently expressed to induce invasive growth...
  4. Morishita T, Mitsuzawa H, Nakafuku M, Nakamura S, Hattori S, Anraku Y. Requirement of Saccharomyces cerevisiae Ras for completion of mitosis. Science. 1995;270:1213-5 pubmed
    ..However, even when the adenosine 3',5'-monophosphate (cAMP) pathway was bypassed, the double disruption of RAS1 and RAS2 resulted in defects in growth at both low and high temperatures...
  5. Toda T, Uno I, Ishikawa T, Powers S, Kataoka T, Broek D, et al. In yeast, RAS proteins are controlling elements of adenylate cyclase. Cell. 1985;40:27-36 pubmed
    ..bcy1 suppresses lethality in ras1- ras2- yeast...
  6. Toda T, Cameron S, Sass P, Zoller M, Wigler M. Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell. 1987;50:277-87 pubmed
    ..The amino-terminal regions show no homology to each other and are heterogeneous in length. The TPK1 gene carried on a multicopy plasmid can suppress both a temperature-sensitive ras2 gene and adenylate cyclase gene. ..
  7. Mosteller R, Park W, Broek D. Analysis of interaction between Ras and CDC25 guanine nucleotide exchange factor using yeast GAL4 two-hybrid system. Methods Enzymol. 1995;255:135-48 pubmed
    ..We recommend that the two-hybrid system be employed in combination with other approaches, including molecular genetic analyses and in vitro binding experiments, for the study of Ras and CDC25-GEF interactions. ..
  8. Jungbluth M, Mösch H, Taxis C. Acetate regulation of spore formation is under the control of the Ras/cyclic AMP/protein kinase A pathway and carbon dioxide in Saccharomyces cerevisiae. Eukaryot Cell. 2012;11:1021-32 pubmed publisher
  9. Swiegers J, Pretorius I, Bauer F. Regulation of respiratory growth by Ras: the glyoxylate cycle mutant, cit2Delta, is suppressed by RAS2. Curr Genet. 2006;50:161-71 pubmed
    ..The data therefore provide new evidence for the role of Ras2p as a powerful modulator of metabolism during growth on a non-fermentable carbon source. ..

More Information


  1. Munder T, Furst P. The Saccharomyces cerevisiae CDC25 gene product binds specifically to catalytically inactive ras proteins in vivo. Mol Cell Biol. 1992;12:2091-9 pubmed
    ..guanine nucleotide exchange proteins Cdc25 and Sdc25 from Saccharomyces cerevisiae bind directly to their targets Ras1 and Ras2 in vivo...
  2. Dong J, Bai X. The membrane localization of Ras2p and the association between Cdc25p and Ras2-GTP are regulated by protein kinase A (PKA) in the yeast Saccharomyces cerevisiae. FEBS Lett. 2011;585:1127-34 pubmed publisher
    ..These results suggest that Ras2p localization and Ras2-GEF activity of Cdc25p play roles in the feedback regulation of Ras2p in the Ras-cAMP pathway. ..
  3. Broek D, Samiy N, Fasano O, Fujiyama A, Tamanoi F, Northup J, et al. Differential activation of yeast adenylate cyclase by wild-type and mutant RAS proteins. Cell. 1985;41:763-9 pubmed
    In these experiments we demonstrate that purified RAS proteins, whether derived from the yeast RAS1 or RAS2 or the human H-ras genes, activate yeast adenylate cyclase in the presence of guanine nucleotides...
  4. Wang Y, Pierce M, Schneper L, Güldal C, Zhang X, Tavazoie S, et al. Ras and Gpa2 mediate one branch of a redundant glucose signaling pathway in yeast. PLoS Biol. 2004;2:E128 pubmed
    ..These studies provide a paradigm for extracting the topology of signal transduction pathways from expression data. ..
  5. Park W, Mosteller R, Broek D. Identification of a dominant-negative mutation in the yeast CDC25 guanine nucleotide exchange factor for Ras. Oncogene. 1997;14:831-6 pubmed
    ..protein bound the mutant RAS2 protein thereby releasing the wild-type CDC25 protein for activation of the wild-type RAS1 protein...
  6. Grandin N, de Almeida A, Charbonneau M. The Cdc14 phosphatase is functionally associated with the Dbf2 protein kinase in Saccharomyces cerevisiae. Mol Gen Genet. 1998;258:104-16 pubmed
    ..2 M sorbitol to the culture medium. Our data are the first to demonstrate a functional link between Cdc14 and Dbf2 based on both biochemical and genetic information. ..
  7. Wilson R, Tatchell K. SRA5 encodes the low-Km cyclic AMP phosphodiesterase of Saccharomyces cerevisiae. Mol Cell Biol. 1988;8:505-10 pubmed
    ..Disruptions of SRA5 allowed ras1 ras2 strains to grow either on rich media supplemented with cAMP or on minimal media without exogenous cAMP...
  8. Créchet J, Cool R, Jacquet E, Lallemand J. Characterization of Saccharomyces cerevisiae Ras1p and chimaeric constructs of Ras proteins reveals the hypervariable region and farnesylation as critical elements in the adenylyl cyclase signaling pathway. Biochemistry. 2003;42:14903-12 pubmed
    ..This strongly suggests that the hypervariable region of Ras proteins can interact directly with adenylyl cyclase. ..
  9. De Vendittis E, Vitelli A, Zahn R, Fasano O. Suppression of defective RAS1 and RAS2 functions in yeast by an adenylate cyclase activated by a single amino acid change. EMBO J. 1986;5:3657-63 pubmed
    ..TS1, with the RAS2 gene replaced by mutant allele encoding a partially defective gene product, and with an inactive RAS1 gene...
  10. Sun J, Kale S, Childress A, Pinswasdi C, Jazwinski S. Divergent roles of RAS1 and RAS2 in yeast longevity. J Biol Chem. 1994;269:18638-45 pubmed
    Individual cells of the yeast Saccharomyces cerevisiae have a limited replicative life-span. The role of the genes RAS1 and RAS2 in yeast longevity was examined...
  11. Li Y, Wang Y. Ras protein/cAMP-dependent protein kinase signaling is negatively regulated by a deubiquitinating enzyme, Ubp3, in yeast. J Biol Chem. 2013;288:11358-65 pubmed publisher
    ..Together, our data indicate that Ubp3 is a new regulator of the Ras/PKA signaling pathway and suggest that Ubp3 regulates this pathway by controlling the ubiquitination of Ras GTPase-accelerating protein Ira2. ..
  12. Tanaka K, Nakafuku M, Satoh T, Marshall M, Gibbs J, Matsumoto K, et al. S. cerevisiae genes IRA1 and IRA2 encode proteins that may be functionally equivalent to mammalian ras GTPase activating protein. Cell. 1990;60:803-7 pubmed
    ..The RAS1 and RAS2 proteins overexpressed in ira mutants accumulated in the GTP-bound form, whereas in the wild-type strain ..
  13. Tatchell K, Robinson L, Breitenbach M. RAS2 of Saccharomyces cerevisiae is required for gluconeogenic growth and proper response to nutrient limitation. Proc Natl Acad Sci U S A. 1985;82:3785-9 pubmed
    ..These two genes, RAS1 and RAS2, constitute an essential gene family since spores with disruptions of both genes fail to grow...
  14. Garrett S, Broach J. Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase. Genes Dev. 1989;3:1336-48 pubmed
    The yeast Saccharomyces cerevisiae contains two functionally redundant genes RAS1 and RAS2, which are homologous to the mammalian ras gene family and are required for vegetative growth...
  15. Marshall M, Gibbs J, Scolnick E, Sigal I. Regulatory function of the Saccharomyces cerevisiae RAS C-terminus. Mol Cell Biol. 1987;7:2309-15 pubmed
    Activating mutations (valine 19 or leucine 68) were introduced into the Saccharomyces cerevisiae RAS1 and RAS2 genes...
  16. Bertazzi D, Kurtulmus B, Pereira G. The cortical protein Lte1 promotes mitotic exit by inhibiting the spindle position checkpoint kinase Kin4. J Cell Biol. 2011;193:1033-48 pubmed publisher
    ..This Lte1-mediated exclusion of Kin4 from the dSPB is essential for proper mitotic exit of cells with a correctly aligned spindle. Therefore, Lte1 promotes mitotic exit by inhibiting Kin4 activity at the dSPB. ..
  17. Feger G, De Vendittis E, Vitelli A, Masturzo P, Zahn R, Verrotti A, et al. Identification of regulatory residues of the yeast adenylyl cyclase. EMBO J. 1991;10:349-59 pubmed
    ..adenylyl cyclase-linked spontaneous mutations capable of suppressing the temperature-sensitive phenotype of ras1- ras2-ts1 strains...
  18. Fujiyama A, Tamanoi F. Processing and fatty acid acylation of RAS1 and RAS2 proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1986;83:1266-70 pubmed
    We demonstrate the pathway for the biosynthesis of RAS1 and RAS2 gene products of Saccharomyces cerevisiae leading to their localization in membranes. The primary translation products of these genes are detected in a soluble fraction...
  19. Powers S, O Neill K, Wigler M. Dominant yeast and mammalian RAS mutants that interfere with the CDC25-dependent activation of wild-type RAS in Saccharomyces cerevisiae. Mol Cell Biol. 1989;9:390-5 pubmed
    ..These results suggest that these mutant RAS genes interfere with the normal interaction of RAS and CDC25 proteins and suggest that this interaction is direct and has evolutionarily conserved features. ..
  20. Rutzler M, Reissaus A, Budzowska M, Bandlow W. SUT2 is a novel multicopy suppressor of low activity of the cAMP/protein kinase A pathway in yeast. Eur J Biochem. 2004;271:1284-91 pubmed
  21. Jin X, Starke S, Li Y, Sethupathi S, Kung G, Dodhiawala P, et al. Nitrogen Starvation-induced Phosphorylation of Ras1 Protein and Its Potential Role in Nutrient Signaling and Stress Response. J Biol Chem. 2016;291:16231-9 pubmed publisher
    b>Ras1 is a small GTPase in the budding yeast Saccharomyces cerevisiae that regulates nutrient signaling...
  22. Whistler J, Rine J. Ras2 and Ras1 protein phosphorylation in Saccharomyces cerevisiae. J Biol Chem. 1997;272:18790-800 pubmed
    ..This site, when mutated to alanine, led to promiscuous phosphorylation of Ras2 protein on nearby serine residues. A decrease in phosphorylation may lead to a decrease in signaling through the Ras-cAMP pathway. ..
  23. Leadsham J, Miller K, Ayscough K, Colombo S, Martegani E, Sudbery P, et al. Whi2p links nutritional sensing to actin-dependent Ras-cAMP-PKA regulation and apoptosis in yeast. J Cell Sci. 2009;122:706-15 pubmed publisher
    ..We also demonstrate for the first time that Whi2p-dependent regulation of cAMP-PKA signalling plays a physiological role in the differentiation of yeast colonies by facilitating elaboration of distinct zones of cell death. ..
  24. Cannon J, Tatchell K. Characterization of Saccharomyces cerevisiae genes encoding subunits of cyclic AMP-dependent protein kinase. Mol Cell Biol. 1987;7:2653-63 pubmed
    Mutations in the SRA1 or SRA3 gene eliminate the requirement for either RAS gene (RAS1 or RAS2) in Saccharomyces cerevisiae. We cloned SRA1 and SRA3 and determined their DNA sequences...
  25. Li J, Li Y, Elledge S. Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association. Mol Cell Biol. 2005;25:767-78 pubmed
    ..Hsk3 is critical for the integrity of the DASH complex because in hsk3 mutants the association of Dam1, Duo1, Spc34, and Spc19 with Ask1 is greatly diminished. We propose that Hsk3 acts to incorporate Ask1 into the DASH complex. ..
  26. Fujimura K, Tanaka K, Toh e A. A dominant interfering mutation in RAS1 of Saccharomyces cerevisiae. Mol Gen Genet. 1993;241:280-6 pubmed
    A mutant allele of RAS1 that dominantly interferes with the wild-type Ras function in the yeast Saccharomyces cerevisiae was discovered during screening of mutants that suppress an ira2 disruption mutation...
  27. Fasano O, Crechet J, De Vendittis E, Zahn R, Feger G, Vitelli A, et al. Yeast mutants temperature-sensitive for growth after random mutagenesis of the chromosomal RAS2 gene and deletion of the RAS1 gene. EMBO J. 1988;7:3375-83 pubmed
    Saccharomyces cerevisiae strains with a disrupted RAS1 gene and with an intact RAS2 gene (ras1- RAS2 strains) grew well on both fermentable and nonfermentable carbon sources...
  28. Kataoka T, Powers S, McGill C, Fasano O, Strathern J, Broach J, et al. Genetic analysis of yeast RAS1 and RAS2 genes. Cell. 1984;37:437-45 pubmed
    We present a genetic analysis of RAS1 and RAS2 of S. cerevisiae, two genes that are highly homologous to mammalian ras genes...
  29. Wei M, Fabrizio P, Hu J, Ge H, Cheng C, Li L, et al. Life span extension by calorie restriction depends on Rim15 and transcription factors downstream of Ras/PKA, Tor, and Sch9. PLoS Genet. 2008;4:e13 pubmed publisher
    ..Notably, the anti-aging effect caused by the inactivation of both pathways is much more potent than that caused by CR. ..
  30. Manandhar S, Hildebrandt E, Jacobsen W, Santangelo G, Schmidt W. Chemical inhibition of CaaX protease activity disrupts yeast Ras localization. Yeast. 2010;27:327-43 pubmed publisher
    ..Together, these results indicate that disrupting the proteolytic modification of Ras GTPases impacts their in vivo trafficking. ..